Suwandi Sugandi
Department of Urology, Faculty of Medicine/Padjadjaran University, Hasan Sadikin Hospital. Jl. Pasteur No. 38 Bandung

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EFFECT OF TESTICULAR TORSION ON SPERMATOZOA IN CONTRALATERAL EPIDIDYMIS Pramod, Sawkar Vijay; Sugandi, Suwandi; Sihombing, Aaron Tigor; Tan, Marselina
Indonesian Journal of Urology Vol 19 No 2 (2012)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v19i2.62

Abstract

Objective: To determine the abnormality of spermatozoa in the contralateral epididymis after unilateral testicular torsion. Material & method: Twenty wistar rats were divided into two groups i.e. Group B (sham procedure) Group A (torsio and orchiectomy 24 hours later), and contralateral epididymectomy was performed a month later. Spermatozoa in the contralateral epididymis are extracted and analyzed by an experienced biologist. Data were analyzed using Chi-square or Fischer exact test. Results: Sperm morphology changes in group B is higher than Group A (6,6% vs 0,5%, p = 0,009). Conclusion: Unilateral testicular torsion causes sperm abnormal morphology in the contralateral epididymis. Keywords: Unilateral testicular torsion, contralateral epididimal spermatozoa.
HUBUNGAN STATUS INSTABILITAS MIKROSATELIT DAN EKSPRESI P53 DENGAN ETIOPATOLOGI ADENOKARSINOMA KOLOREKTAL PADA ORANG INDONESIA DI KELOMPOK USIA KURANG DARI 40 TAHUN Lukman, Kiki; Dewayani, Brigitta M.; Hernowo, Bethy S.; Hanafi, Basrul; Achmad, Tri Hanggono; Sugandi, Suwandi
Indonesian Journal of Applied Sciences Vol 2, No 1 (2012)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8084.818 KB) | DOI: 10.24198/ijas.v2i1.2730

Abstract

Epidemiologically, the percentage of colorectal adenocarcinoma (CRC) in the age group ≤40 years old in Indonesia is higher than in the West European, North American and other developed Asian countries with different clinico-pathological features. In the afromentioned countries, the carcinogenesis pathway of this group is hereditary which show high microsatellite instability (MSI), whereas the age group >40 years old is sporadic of which show chromosomal instability (CIN) with the mutation of p53 gene. To elucidate the carcinogenesis pathway with its molecular characteristics of this group among Indonesians, a cross sectional study was conducted by performing the immunohistochemical detection of MLH1 and MSH2 expression (MSI status), and mutated p53 gene expression (CIN) in the CRC group ≤40 years old and >40 years old at Dr. Hasan Sadikin Hospital, Bandung, from May 2008 until November 2009. Thirty nine CRC patients, consisting of 22 patients of  ≤40 years old and 17patients >40 years old, were eligible for this study. The CRC patients of ≤40 years old showed 4 MSI high,  1 MSI lowand 17 MSS (microsatellite stable), associated with 10 p53 positive and 12 p53 negative status. In the CRC group of >40 years old, there was no MSI high, but 4 MSI low and 13 MSS were found, associated with 11 p53positive and 6 p53negative status. There was no significant difference with regards to the association between MSI status and p53 expression in both groups (p MSI=0.95, p p53=0.23).
ULTRASONOGRAFI UNTUK PREDIKSI LASERASI PARENKIM GINJAL DAN HEMATOMA PERIRENAL PADA PASIEN TRAUMA TUMPUL ABDOMEN DENGAN KECURIGAAN TRAUMA GINJAL Richata, Galuh; Sugandi, Suwandi; Haroen, Zulhardi; E, Mumuh M; Noegroho, Bambang S; Tjahjodjati, Tjahjodjati; Safriadi, Ferry
Indonesian Journal of Urology Vol 15 No 1 (2008)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v15i1.366

Abstract

Objective: To evaluate the sensitivity and the specificity of the ultrasound in predicting renal parenchyma laceration and perirenal hematoma in patients with abdominal blunt trauma with suspicion of renal trauma, as confirmed by operative findings in the explorative laparotomy. Material & methods: From March 2005 to March 2006, we found 28 patients admitted to the emergency department with abdominal blunt trauma with suspicion of renal trauma. Every patient was scanned sonografically to detect the presence of the renal parenchyma laceration and perirenal hematoma. All those patients underwent laparotomy for any certain indication, but not because of ultrasound findings. The ultrasound findings then were checked with the operative findings. Results: The sensitivity of the ultrasound in predicting renal parenchyma laceration is 53.8%, while its sensitivity in predicting perirenal hematoma is 75%. The specificity of ultrasound in predicting both renal parenchyma laceration and perirenal hematoma is 100%. Conclusion: Ultrasound for kidney trauma is sensitive in predicting the presence of perirenal hematoma, but not sensitive in predicting the presence of a renal parenchyma laceration. Ultrasound is very specific in predicting if both perirenal hematoma and renal parenchyma laceration are present.
PENGETAHUAN, TINGKAH LAKU, DAN TINDAKAN KLINIS DOKTER UMUM PADA PENATALAKSANAAN BPH Adi, Kuncoro; Safriadi, Ferry; Sugandi, Suwandi; Haroen, Zulhardi; Noegroho, Bambang S; Tjahjodjati, Tjahjodjati
Indonesian Journal of Urology Vol 15 No 1 (2008)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v15i1.362

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Objective: To evaluate the Primary Care Physician’s (PCPs) knowledge, attitude and clinical behavior in managing patients with BPH, as an input in the review of BPH guideline of Indonesian Urology Association. Material & method: The mailing included a cover letter, questioner consist of 10 questions survey, survey instrument and a post-paid envelope. A 10-questions survey were validated according to BPH guideline by Indonesian Urology Association (IUA). PCPs who work in district health offices and registered in district health office of Indonesian Health Ministry in Bandung, were selected as respondents. Results: 75% of sampled primary care physician working in 71 district health offices responded. 83% of respondent did not know the BPH guideline that had been published by Indonesian Urology Association. Although 88% of PCP’s knew about the symptoms of BPH, only 32% heard about of International Prostate Symptoms score. However, only 13 % were reported applying the symptoms score. Regarding to mandatory examination by the guideline, 64% of PCPs reported performing digital rectal examination, and 47% reported ordering urinalysis test. The recommended test such as PSA and serum creatinine level only asked 24% and 60% by PCPs. Only 21% of PCPs knew about the uroflowmetry but never ordering the test. 47% of PCPs reported using α blockers and 2% using finasteride for treating BPH. The referral was conducted by PCPs mostly due to urinary retention and hematuria. 70% of PCPs would refer the patients to urologist and the others to general surgeon and general internist. Conclusion: Although PCPs play role up to certain extent in diagnosing and managing of BPH, most of them are not interested in looking for reference.
LITOTRIPSI LASER HOLMIUM YAG UNTUK TERAPI BATU URETER Adi, Kuncoro; Safriadi, Ferry; Sugandi, Suwandi; Haroen, Zulhardi; Noegroho, Bambang S; Tjahjodjati, Tjahjodjati
Indonesian Journal of Urology Vol 15 No 2 (2008)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v15i2.352

Abstract

Objective: To assess the efficacy of pulsed holmium:YAG laser lithotripsy for ureteral stone therapy. Material and method: Ninety-one patients (70 males and 21 females) with age range 20 – 76 years underwent 93 ureteroscopic procedures for 101 ureteral stones. A preoperative diagnosis was established by ultrasound scanning and intravenous urography. An 8 F rigid Karl-Storz ureteroscope was used for a holmium:YAG laser (OmniPulse MaxTM 80 watt Holmium Laser System Model 1210-VHP, Trimedyne,Inc. Irvine CA, USA) as energy source for laser lithotripsy. The stone localization was mostly at the lower third of the ureter. The mean stone size was 9,6 mm, mean duration of procedure was 48,1 minutes, and the mean hospital stay was 2,1 days. Results: The overall stone clearance rate was 95,6% with the best results for stones in the middle third of the ureter (100%). At follow up we noted two patients with residual stones and two patients with a perforation of the ureteral wall. Conclusion: The Holmium:YAG laser is effective for ureteral stone treatment with a high success rate. Special attention and care should be afforded in impacted ureteral stones. 
ESTROGEN EFFECT ON WISTAR'S VAGINAL EPITHELIUM Surur, Abu; Sugandi, Suwandi; Haroen, Zulhardi; Noegroho, Bambang S; Tjahjodjati, Tjahdjojati; Safriadi, Ferry; Siregar, Safendra; Adriansjah, Ricky; Adi, Kuncoro; Hernowo, Betthy S
Indonesian Journal of Urology Vol 17 No 2 (2010)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v17i2.349

Abstract

Objective: To evaluate the effect of estrogen on wistar’s vaginal epithelium. Material & method: We divided 30 female wistars into three groups, ten wistars underwent bilateral ovariectomy, 10 wistars underwent bilateral ovariectomy, and estrogen replacement and 10 wistars as control. The second group received estradiol 1 mg/kg/day, directly after ovariectomy. After 4 weeks the vagina was then harvested and stained with hematoxylin-eosin to evaluate the thickness of epithelial layer of vagina. A pair T-test was use for statistical analysis. Results: Estrogen ablation decreased the thickness of epithelial layer in wistar’s vagina. The mean thickness of epithelial layer in vagina were 3, 5, and 10 for each group respectively. The mean epithelial layer of the first group as well as the second group showed significant difference compared to control group (p>0,05), while the first group showed insignificant difference compared to the second group. Conclusion: Estrogen ablation seems to decrease the thickness of vaginal epithelium, while estrogen replacement revealed insignificant effect.
ANGIOTENSIN II TYPE-1 RECEPTOR (AT1R) DISTRIBUTION IN BPH, HIGH GRADE PIN AND ADENOCARCINOMA OF THE PROSTATE Septiadi, Isdianto; Sugandi, Suwandi; Noegroho, Bambang S; Tjahjodjati, Tjahjodjati; Safriadi, Ferry; Siregar, Safendra; Adriansjah, Ricky; Adi, Kuncoro; Sihombing, Aaron T; Pramod, Sawkar V; Nasution, Ramlan; Hernowo, Betthy S
Indonesian Journal of Urology Vol 17 No 2 (2010)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v17i2.348

Abstract

Objective: The aim of the study was to detect the differences in number and the distribution of angiotensin II type-1 receptor (AT1R) in BPH, high grade PIN, and adenocarcinoma of the prostate. Material & method: A prospective study was performed in RSHS, in collaboration with the Department of Anatomical Pathology. Prostate samples were taken by TUR of the prostate, and then divided into 5 groups. They were BPH, high grade PIN, adenocarcinoma of the prostate in 3 difference grades (well, moderate, and poorly differentiated). Kidney tissue for control. Immunohistochemical staining was done to determine the angiotensin II type-1 (AT1R) receptor distribution as primary antibody used was mouse monoclonal antibody AT1 (TONI-1): sc-57036, Santa Cruz Biotechnology Inc., CA. Results: Angiotensin II type-1 receptor was found in material of BPH, high grade PIN and adenocarcinoma of the prostate. The number and distribution of the receptors were not different. Conclusion: There are no significant differences in number and distribution of angiotensin II type-1 receptor on BPH, high grade PIN, and adenocarcinoma of the prostate.
ATHEROSCLEROTIC PLAQUE OF RENAL VASA RECTA ON PATIENT WITH CALCIUM STONE Pramod, Sawkar Vijay; Sugandi, Suwandi; Sihombing, Aaron T.; Makmuri, Makmuri
Indonesian Journal of Urology Vol 20 No 1 (2013)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v20i1.45

Abstract

Objective: We would like to identify whether atherosclerotic plaque inside renal vasa recta correlates with the formation of calcium stone. Material & Method: Samples are taken from kidney tissue removed by nephrectomy due to stone and non-stone disease. Histopathological examination with specific staining was performed by a single pathologist to find the atherosclerotic plaques inside the renal vasa recta. The result of each group was compared, and then analyzed using the Chi square test/Fischer exact test (SPSS ver.16.0; p < 0,05). Results: We found that the risk of calcium lithiasis is two-fold higher in the group with atherosclerotic plaques inside the renal vasa recta. (PR = 2,15; p < 0,05). Conclusion: Calcium stone are more likely to occur on patients with renal vasa recta atherosclerotic plaque, as possible sequelae of vascular injury.Keywords: Atherosclerotic plaque, vasa recta, calcium renal stone.
COMPARISON BETWEEN GLYCOGEN LEVEL OF KIDNEY TUBULAR CELLS IN DIABETIC KIDNEY STONE PATIENTS AND NON DIABETIC KIDNEY STONE PATIENTS Ramadhan, Ken; Sugandi, Suwandi; Noegroho, Bambang S; Tjahjodjati, Tjahjodjati; Safriadi, Ferry; Siregar, Safendra; Adriansjah, Ricky; Adi, Kuncoro; Sihombing, Aaron Tigor; Yusuf, Makmuri
Indonesian Journal of Urology Vol 18 No 1 (2011)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v18i1.76

Abstract

Objective: To compare glycogen level of kidney tubular cells in diabetic kidney stone patients with nondiabetic kidney stone patients. Material & method: We reviewed kidney stone patients who underwent nephrectomy in Hasan Sadikin Hospital from February 2008 to January 2009. Age, gender, type of DM, and urine pH were recorded. Glycogen level of kidney tubular cells were evaluated histochemically using HE, PAS, and PAS diastase. The results were categorized into 3 grades, based on the staining appearance compared to liver cells as controls. The results of diabetic group were compared to non diabetic patients. Results: There were 30 patients eligible for this study, 15 in diabetic group and 15 non diabetic patients. In the diabetic group, there were 10 patients with urine pH < 6 and 5 patients with a pH of 6,5. In the non diabetic group all urine pH were > 6,5. Examination revealed that glycogen level of kidney tubular cells in diabetic group was higher than in non diabetic group. Conclusion: Glycogen level of kidney tubular cells in diabetic kidney stone patients was higher than in non diabetic kidney stone patients. Keywords: Tubular cell, kidney stone, diabetes mellitus.
THE EFFECT OF PREDNISONE ON SERTOLI CELL OF CONTRALATERAL TESTICULAR TORSION Tanuwidjaja, Dandy; Sugandi, Suwandi; Sihombing, Aaron Tigor Tigor; Yantisetiasti, Anglita; Hernowo, Betthy S.
Indonesian Journal of Urology Vol 19 No 2 (2012)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v19i2.58

Abstract

Objective: To evaluate the effect of prednisone in unilateral testicular torsion on Sertoli cell quality of contralateral testis. Material &amp; Method: Thirty Wistar rats were divided into three groups i.e. group A (sham procedure), group B (unilateral torsion + orchiectomy after 6 hours), and group C (unilateral torsion + orchiectomy after 24 hours). Group B and C were further divided into subgroup with and without Prednisone administration. Prednisone was given orally once a day an hour after torsion, continued till one month later. Orchiectomy of contralateral testis was performed one month later. Those testes were examined by a pathologist. Results: In group A, no abnormality on Sertoli cell quality was found. There is significant difference among groups in Sertoli cell quality (p = 0,01). Ischemic time is associated with Sertoli cell quality (2 rats with severe damage in group C and none in group B). In group B and C, prednisone administration inhibited Sertoli cell damage. Prednisone administration in 6 hours group gave better results than 24 hours group (3 rats vs 1 rat with good Sertoli cell quality). Conclusion: Sertoli cell in contralateral testis is significantly affected by unilateral testicular torsion. Prednisone inhibit Sertoli cells damage. Ischemic time affected Sertoli cell quality of contralateral testis and respond to prednisone.Keywords: Prednisone, unilateral testicular torsion, sertoli cell.