SRIANI SUJIPRIHATI
Departemen Agronomi dan Hortikultura, Institut Pertanian Bogor, Jl. Meranti, Kampus IPB Darmaga, Bogor 16680

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KETAHANAN BIOKIMIA TANAMAN CABAI TERHADAP BEGOMOVIRUS PENYEBAB PENYAKIT DAUN KERITING KUNING Faizah, Rokhana; Sujiprihati, Sriani; Syukur, Muhamad; Hidayat, Sri Hendrastuti
Jurnal Fitopatologi Indonesia Vol 8 No 5 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.548 KB) | DOI: 10.14692/jfi.8.5.138

Abstract

Plant biochemical resistance towards virus infection can be evaluated through measurement of salicylic acid accumulation and peroxidase concentration. A research was conducted to study biochemical resistance of chillipepper to infection of Pepper yellow leaf curl Begomovirus (PYLCV) the causal agent of pepper yellow leaf curl disease. Virus inoculation was done using insect vector, Bemisia tabaci. Salicylic acid accumulation and peroxidase concentration on 6 chillipepper genotypes (35C2, IPB C15, IPB C26, IPB C10, IPB C14, dan IPB C12) was measured 120 h after inoculation. The results showed that salicylic acid accumulation and peroxidase concentration were higher on plants after virus infection. Susceptible genotypes (35C2, IPB C15, IPB C26) tend to have higher salicylic acid accumulation and peroxidase concentration than those of resistant genotype (IPB C12). Positive correlation was observed between biochemical resistance (salicylic acid accumulation) and structural resistance (length of palisade meshophyll cell).Key words: Begomovirus, Pepper yellow leaf curl virus, peroxide enzyme, resistance mechanism, salicylic acid
PENGARUH PERLAKUAN ETHYL METHANE SULFONATE PADA TANAMAN CABAI (CAPSICUM ANNUUM L.) DAN KETAHANANNYA TERHADAP CHILLI VEINAL MOTTLE VIRUS (CHIVMV) Manzila, Ifa; Hidayat, Sri Hendrastuti; Mariska, Ika; Sujiprihati, Sriani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 3 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.474 KB) | DOI: 10.24831/jai.v38i3.14250

Abstract

Ethyl Methane Sulfonate (EMS) may induce mutation leading to somaclonal variation if it is used at the appropriate combination of EMS concentration and exposure time. Variation in somaclonal might be valuable as a source of resistance to plant pathogens including plant viruses. This study was aimed 1) to determine the optimum EMS concentration and incubation time that may induce somaclonal variation in chilli pepper; and 2) to evaluate the resistance of the somaclone to ChiVMV infection. Shoot-tip explants of fi ve chilli pepper genotypes (Jatilaba, ICPN 12#4, PBC495, Helem, and Gelora) were treated with EMS at combination of different concentrations (0.25%, 0.5% 1.0% and control), and incubation time (15, 30, 60 min). Subsequently, each explant was grown in multiplication media (MS media + 5 mg L -1 BAP + 0.5 mg L-1 TDZ), rooting media (MS media + 1 mg L-1 NAA), and acclimatization media (mixture of soil : sand : compost 2:1:1 w/w). Our results showed that the higher EMS concentration and the longer incubation period the smaller the number of survive explants. The highest survival rate 20.4 % was achieved with 0.5% EMS in combination with 60 min incubation period. This treatment combination also showed induction of phenotypic variation. Two somaclonal plants derived from Gelora genotype, designated as somaclones K1 and K2, survived until fruit development and maturation. A total of 245 progenies of K1 and 243 progenies of K2, respectively were evaluated for their resistance to ChiVMV infection through mechanical inoculation using ChiVMV-Cikabayan isolate. Following the detection of ChiVMV using DAS-ELISA, it was confi rmed that 4.09% of the somaclonal progenies were resistance to ChiVMV. Keywords: Capsicum annuum L., ChiVMV, ethyl methane sulfonate, induce mutation, resistance
METODE PENGUSANGAN CEPAT UNTUK PENGUJIAN VIGOR DAYA SIMPAN BENIH CABAI (CAPSICUM ANNUUML.) Ekowahyuni, Luluk Prihastuti; Sutjahjo, Surjono Hadi; Sujiprihati, Sriani; Suhartanto, Mohamad Rahmad; Syukur, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.268 KB) | DOI: 10.24831/jai.v40i2.6374

Abstract

The ability of seed to maintain seed quality during storage is called seed vigor. Accurate method for seed vigor testing of pepper seeds is necessary to accurately determine seed storability during seed distribution. The aim of this research was to determine the best accelerated aging method (AAM) of pepper seed. Freshly harvested seeds of Capsicum annuumL., IPB C9 genotype were used in this study. The experiment used randomized complete block design (RCBD) with three replications. The experimental treatments were seed vigor testing methods i.e. natural deterioration test at room temperature in controlled humidity (RH 90-95%), and four AAM (hot water 60 ºC, methanol 20%, ethanol 20%, and high temperature at 40 ºC). The best method was selected using analysis of variance, coeficient of variance, regresion dan t student analysis. AAM using 20% methanol at periods of 0, 2, 4, 6 and 8 hours was the best method of seed vigor testing for pepper seed. Keywords: accelerated aging, deterioration, ethanol, methanol, high temperature
SELECTION METHOD IS ONE OF THE MOST IMPORTANT FACTORS IN DETERMINING THE SUCCESS OF PEPPER BREEDING PROGRAMS. SELECTION METHOD WILL BE EFFECTIVE IF IT IS SUPPORTED BY A COMPLETE KNOWLEDGE OF GENETIC CHARACTER INHERITANCE. THE AIM OF THIS RESEARCH WAS TO INVESTIGATE THE INHERITANCE PATTERNS TO QUANTITATIVE CHARACTERS USING LARGE PEPPER (P1 (IPB C5)), CURLY PEPPER (P2 (IPB C105)), F1, F1R, BCP, BCP2 AND F2. THE RESULT SHOWED THAT DICHOTOMOUS HEIGHT, DAYS TO HARVEST AND WEIGHT PER FRUIT CHARACTERS Arif, Abdullah Bin; Sujiprihati, Sriani; Syukur, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v40i2.6359

Abstract

Selection method is one of the most important factors in determining the success of pepper breeding programs. Selection method will be effective if it is supported by a complete knowledge of genetic character inheritance. The aim of this research was to investigate the inheritance patterns to quantitative characters using large pepper (P1 (IPB C5)), curly pepper (P2 (IPB C105)), F1, F1R, BCP, BCP2 and F2. The result showed that dichotomous height, days to harvest and weight per fruit characters were not maternally inherited. The additive-dominant genetic model was the most suitable for dichotomous height character. The additive-dominant with influence of interaction additive-dominant and interaction dominant-dominant genetic model was suitable for days to harvest character. The additive-dominant with influence of interaction additive-additive and interaction dominant-dominant genetic model was suitable for the weight of fruit character. Broad-sense and narrow-sense heritabilities range medium for dichotomous height and weight per fruit characters. Heritability of the days to harvest character was high in a broad-sense but was low in the narrow-sense. Keywords: genetic model, heritability, inheritance
ANALISIS KEDEKATAN HUBUNGAN ANTAR GENOTIPE PEPAYA BERDASARKAN KARAKTER MORFOLOGI DAN BUAH Suketi, Ketty; Poerwanto, Roedhy; Sujiprihati, Sriani; Sobir, ,; Widodo, Winarso D.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.631 KB) | DOI: 10.24831/jai.v38i2.1797

Abstract

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> A study was conducted to determine the variation and relationships among  papaya genotypes based on morphological and fruit characteristics  in order to produce  high quality papaya fruits. Fruit characterization study is very useful for genotype improvement and genotype classi?  cation of papaya. In this study the morphological characters of 36 genotypes were analyzed to determine their phenotypic variabilities.  The relationships between genotypes based on all of the morphological and fruit characteristics were tested by subjecting the data to multivariate principal component analysis and to cluster analysis. Based on the dendrogram generated from vegetative and generative characters, the 36 genotypes could be grouped into 11 clusters on a threshold of 1.6 and formed 6 clusters on a threshold of 1.8.  The dendrogram was able to explain the close relationship between IPB 5 x IPB 1 and IPB 5 x IPB 4, IPB 2 and IPB 7, IPB 1 and IPB 3 genotype. The scattered diagram of generative variable divided the papaya genotypes into three groups based on fruit sizes i.e small group (IPB 1, IPB 3, IPB 4, IPB 3 x IPB 4, IPB 1 x IPB 9), medium group (IPB 5, IPB 7, IPB 8, IPB 9) and big group (IPB 2, IPB 10).  The IPB 1, IPB 3 and IPB 4 were different from IPB 2 in fruit shapes, petal length of male ?  owers, in? orescence size and fruit length. The hybrid plants obtained from crossings with IPB 10 were different from the other genotypes in the colours of female-, hermaphrodite-, and male ?  ower-lobes. Subsequently the scatter diagrams also revealed that several genotypes i.e. IPB 2 x IPB 6, IPB 1 x IPB 5,  IPB 1 x IPB 9, IPB 5 x IPB 1 and IPB 5 x IPB 2 had superior characters  ideotype similar to IPB 1, IPB 3 and IPB 8 genotypes. Keywords: Carica papaya, hermaphrodite, female, dendrogram, scatter diagram, ideotype
KRITERIA SELEKSI UNTUK PERAKITAN VARIETAS CABAI TAHAN PHYTOPHTHORA CAPSICI LEONIAN Yunianti, Rahmi; Sastrosumarjo, Sarsidi; Sujiprihati, Sriani; Surahman, Memen; Hidayat, Sri Hendrastuti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.721 KB) | DOI: 10.24831/jai.v38i2.1796

Abstract

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Selection criteria for developing Phytophthora blight-resistant varieties of pepper had been done in both laboratory and ?  eld conditions. Resistance screening were conducted on 28-days-old pepper plants grown in 72-cell ?  ats by inoculating 5 mL of inoculum (contain 105 zoospore mL-1) to the base of each plant. Phytophthora capsici isolate used in this experiment was TG01, which then identi?  ed as race-3. The evaluation of pepper genotypes characteristics were conducted in ?  eld.  Eight characters demonstrated a wide genetic variability; those characters were resistance to Phytophthora blight, infection period, dicotomous height, canopy width, fruit weight, fruit length, fruit diameter and production. These characters along with fruit wall thickness, chlorophyll-a, chlorophyll-b, total chlorophyll had high heritability values. Using path analysis infection period, dicotomous height and fruit diameter are recommended to be simultaneously used as selection criteria for developing Phytophthora blight resistant pepper varieties. Keywords : capsicum, Phytophthora capsici, selection criteria, path analysis
HETEROSIS DAN DAYA GABUNG KARAKTER AGRONOMI CABAI (CAPSICUM ANNUUM L.) HASIL PERSILANGAN HALF DIALLEL Daryanto, Ady; Sujiprihati, Sriani; Syukur, Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.704 KB) | DOI: 10.24831/jai.v38i2.1795

Abstract

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> The objective of this research was to study heterosis and heterobeltiosis effects of ?  fteen chili genotypes (Capsicum annuum L.), the general combining ability (GCA) and speci?  c combining ability (SCA) of six chili inbred lines through half diallel crosses. The experiment was conducted from November 2008 to June 2009 using a Randomized Complete Block Design with three replications at IPB experimental ?  eld, Leuwikopo, Darmaga. Heterosis values were predicted based on the average values of their parents whereas heterobeltiosis were predicted based on the average values of the highest parents. Analyses GCA and SCA were based on the Grif?  ng’s ? xed model of diallel design method II. Genotype IPB C2 had  the highest GCA for fruit weight and fruit length. Genotype IPB C15 had the highest GCA for yield per plant and percentage of marketable fruit. Hybrid IPB C2 x IPB C14 and IPB C9 x IPB C14 had the highest heterosis, heterobeltiosis, and SCA for all fruit characters and yield per plant. Keywords: chili, hybrid, heterobeltiosis, yield.
EVALUASI DAYA HASIL CABAI HIBRIDA DAN DAYA ADAPTASINYA DI EMPAT LOKASI DALAM DUA TAHUN Syukur, Muhamad; Sujiprihati, Sriani; Yunianti, Rahmi; Kusumah, Darmawan Asta
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.75 KB) | DOI: 10.24831/jai.v38i1.1679

Abstract

The aim of the experiment was to study the performance of yield components of  eight pepper hybrids and their adaptation at four locations in two years (2008 and 2009). The experimental design used was Randomized Complete Block Design (RCBD), three replications as a block was nested in the location. Plant materials were eight hybrids (IPB CH1, IPB CH2, IPB CH3, IPB CH25, and four commercial hybrids were Adipati, Biola, Gada, and Hot Beauty). The IPB CH3 hybrid produced higher fruit width, fruit length, fruit weight, fruit flesh density and yield per plant compared to commercial hybrids. Days to flowering and days to harvesting of IPB CH3 was earlier than that of Hot Beauty. The performance of yield characters in Boyolali, Rembang and Subang was better than that in Bogor. The IPB CH3 hybrid was specifically adapted to Subang and Rembang, while IPB CH1, IPB CH2, IPB CH25 were suitable for Boyolali.   Keywords: fruit, yield components, genotype x location interaction, Capsicum annuum L.
KARAKTER FISIK DAN KIMIA BUAH PEPAYA PADA STADIA KEMATANGAN BERBEDA Suketi, Ketty; Poerwanto, Roedhy; Sujiprihati, Sriani; Sobir, ,; Widodo, Winarso Drajad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.04 KB) | DOI: 10.24831/jai.v38i1.1678

Abstract

The objective of the experiment was to investigate the physical and chemical characteristics of three stadia of maturity based on a range of peel color from green to yellow or based on percentage of the yellow area of fruit peel (stadium 1 = 25-49 % yellow, stadium 2 = 50-74 % yellow, and stadium 3 = above 75 % yellow) on six genotypes of papaya. Each genotype exhibited different days to maturity for each stadium. The fruits of stadium 1, 2 and 3 for IPB 1 were picked at 130, 135, and 140 days after anthesis (DAA); IPB 10A at 160, 165, and 170 DAA;  IPB 1 x PB 174 at 135, 140 and 145 DAA; while PB 174,  IPB 1 x IPB 10A and IPB 10A x PB 174 were picked at 140, 145 and 150 DAA, respectively. The results indicated that peel firmness was affected by maturity stage on female fruit of IPB 10A. Maturity stage affected chemical characteristics of papaya included total soluble solids (TSS) content (IPB 10A, female fruit of  PB 174, female fruit of IPB 1 x IPB 10A, and female fruit of IPB 1 x PB 174), vitamin C content (hermaphrodite fruit of 10 A, female fruit of IPB 1 x IPB 10A) and juice pH (hermaphrodite fruit of  IPB 1).  IPB 1 genotype can be harvested at all stadia of maturity stage. Hermaphrodite and female fruit of IPB 10 A, female fruit of  PB 174, female fruit of  IPB 1 x IPB 10A and female fruit of IPB 1 x  PB 174 genotype would be better harvested at stadium 3 of maturity stage.   Keywords: Carica papaya, papaya genotype, hermaphrodite fruit, female fruit, fruit quality, fruit maturity stage
DEVELOPMENT OF SCAR MARKER FOR DETECTION OF SEX EXPRESSION IN PAPAYA (CARICA PAPAYA L.) FROM SEVERAL GENETIC BACKGROUNDS Sobir, ,; Sujiprihati, Sriani; Pandia, Evalina C.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.875 KB) | DOI: 10.24831/jai.v36i3.1382

Abstract

Papaya plants are hermaphrodite, pistillate, or staminate. Sex inheritance in papaya is determined by a single gene locus with three alleles of M which is dominant for maleness, MH for hermaphrodites and m which is recessive for femaleness. Only fruits from hermaphrodite plants are marketed since they have the necessary commercial characteristics, i.e., they are pear-shaped and have thicker flesh and a smaller internal cavity. Increased papaya yield has been limited mainly by the ratio of female to hermaphrodite (1: 2) plants normally occurring in orchards. This ratio causes great losses to papaya producers. Identification of seedlings sex during nursery stage is of prime iportance. In order to obtain simple DNA markers to identify sex expression in papaya, five SCAR markers of 20-21 primers were utilized. Examination of these markers into 24 genotypes of papaya from 12 populations of different genetic background revealed that pair of primer PKBT-5 had successfully differentiated male and hermaphrodite plants from female plants. Hence, PKBT-5 pair of primer can be utilized as DNA marker for sex expression character identification in papayas.   Key words: Papaya, sex expression, SCAR marker