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Journal : Indonesian Aquaculture Journal

NONSPECIFIC IMMUNE RESPONSE AND RESISTANCE OF Litopenaeus vannamei FED WITH NUCLEOTIDE, β-GLUCAN, AND PROTAGEN DIETS Manoppo, Henky; Sukenda, Sukenda; Djokosetiyanto, Daniel; Sukadi, Mochamad Fatuchri; Harris, Enang
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.141 KB) | DOI: 10.15578/iaj.5.1.2010.37-44

Abstract

The objective of this research was to evaluate the nonspecific immune response and resistance of Litopenaeus vannamei fed with nucleotide, β–glucan, and protagen diets. Shrimp juveniles with an average weight of 5.39±0.56 g were reared in glass aquaria at a density of 15 shrimps/aquarium. Shrimps were fed three times a day for four weeks at a feeding rate of 3%/bw/day. Treatment diets consisted of A: basal diet (without immunostimulant), B: β–glucan, C: protagen, and D: nucleotide, each with three replicates. At the end of feeding period, the shrimps were intramuscularly injected with Vibrio harveyi 0.1 x 106 cfu.shrimp-1. Total haemocyte count (THC) of shrimp fed with nucleotide-diet was significantly different compared to that of control shrimp (p=0.01), but not different compared to shrimp fed with protagen-diet. PO activity also increased significantly in shrimp fed with nucleotide-diet (p=0.02). β–glucan diet could also increase THC and PO activity, but compared to the control, the increase was not significantly different. Overall, PO activity of shrimp fed with nucleotide, β–glucan, and protagen diets was high (>0.35). Oral administration of nucleotide, β–glucan, and protagen for four consecutive weeks significantly increased resistance of shrimp to disease (<0.01) where the highest resistance rate was observed on shrimp fed with nucleotide-diet. Growth of shrimp fed with nucleotide-diet was significantly different compared to that of control shrimp (p<0.01), as well as to β–glucan, and protagen-treated shrimp. As a conclusion, supplementation of nucleotide into shrimp pellet enhanced nonspecific immune response and growth performance better than β-glucan, and protagen.
CLONING OF ProAV PROMOTER ISOLATED FROM TIGER PRAWN Penaeus monodon Parenrengi, Andi; Alimuddin, Alimuddin; Sukenda, Sukenda; Sumantadinata, Komar; Yamin, Muhammad; Tenriulo, Andi
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.086 KB) | DOI: 10.15578/iaj.4.1.2009.1-7

Abstract

Promoter is a specific DNA sequence involved in the transcription of a particular gene. It is usually located in the upstream of the gene they regulate. Isolation and characterization of promoter is essentially needed in order to establish the sequence analysis and transcription factor that are used in the regulation of gene expression. The research was conducted to analyze the characteristics of Penaeus monodon anti viral gene promoter (ProAV) towards generation of auto-transgenic tiger prawn, P. monodon. ProAV promoter was isolated by PCR (Polymerase Chain Reaction) method and the purified DNA fragment was cloned into pGEM-T Easy cloning vector. The promoter sequence was characterized by using BLAST-N and Genetyx version 7 softwares. The results showed the success in isolating a promoter from tiger prawn of 368 bp in length. BLAST-N analysis showed that the sequence of isolated promoter has high similarity (95%-98%) compared to the other promoters in the GeneBank. The study revealed the existence of important transcription factors (TATA box, MRE, TCF-1, and other potential regulatory elements) are identified in the promoter sequence.
CARBON TO NITROGEN RATIO AND NITROGENOUS WASTE ACCUMULATION IN THE INTENSIVE CATFISH (Clarias gariepinus) CULTURE Gunadi, Bambang; Harris, Enang; Supriyono, Eddy; Sukenda, Sukenda; Budiardi, Tatag
Indonesian Aquaculture Journal Vol 6, No 1 (2011): (June 2011)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.145 KB) | DOI: 10.15578/iaj.6.1.2011.19-29

Abstract

This experiment was carried out to determine the optimum C/N ratio for heterotrophic bacteria (biofloc) growth in order to control nitrogenous waste accumulation in the catfish (Clarias gariepinus) culture. Twenty fish with an initial individual size of about 50 g were stocked in fiberglass tanks which were filled with 200 L of water. Fish were fed with commercial floating fish feed with a protein level of 31%-33% (manufacturer label). The daily feeding rate was 2.5% of the fish biomass. The inoculation of commercial Bacillus sp. isolates was applied in the first day of the experiment after fish stocking in order to obtain a bacterial density in water of 106 cfu/L. Molases was suplemented daily to the tanks to adjust C/N ratio in water. Four C/N ratios, i.e. 0, 7, 14, and 21, were applied as treatments in this experiment. The results showed that molasses suplementation up to C/N ratio 14 to 21 were able to support the growth of heterotrophic bacteria and to inhibit the accumulation of total ammonia nitrogen (TAN) and nitrite in water therefore increase water quality for better growth of cultured catfish.
SPECIFICITY OF Haemolysin AND Gyrase GENE MARKER FOR RAPID DETECTION OF VIBRIOSIS ON PENAEID SHRIMP Kadriah, Ince Ayu Khairana; Sukenda, Sukenda; Yuhana, Munti; Susianingsih, Endang; Atmomarsono, Muharijadi
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (918.882 KB) | DOI: 10.15578/iaj.8.1.2013.47-54

Abstract

Pathogenic Vibrio spp. have been implicated as being one of the major causes of the disease problems in penaeid shrimp culture. For many pathogens, the outcome of the interaction between host and bacterium is strongly affected by the bacterial population size. Efforts for rapid molecular detection is to isolate specific genes which was owned by the pathogenic luminescent Vibrio and used it as the marker molecular in the rapid diagnosis of the disease. Haemolysin and gyrase gene known as one of the specific genes possessed pathogenic bacteria including Vibrio. The objectives of this study were to determine the specificity of designed specific primer from local isolate. Specificity test was performed for haemolysin primer (IAVhF1 and IAVhR1), gyrase primer (IAGyrF1 and IAGyr2) and commercial detection kit. The specificity test comparing the PCR result of pathogenic Vibrio DNA template as positive control, and DNA template from non pathogenic luminescent Vibrio, as well as pathogenic non Vibrios. The result showed that at annealing temperature of 60°C, haemolysin primer was more specific in detecting pathogenic Vibrio for penaeid shrimp than that of gyrase primer and commercial detection kit.
Co-Authors Achmad Suhermanto Aditya, Tiya Widi Alimuddin Alimuddin Amrullah Amrullah Andi Parenrengi Andi Tenriulo ANGELA MARIANA LUSIASTUTI Atmomarsono, Muharijadi Avarre, Jean-Christophe Ayi Santika Azis Azis, Azis Bambang Gunadi Brite, Margie Daniel Djokosetiyanto Dedi Herdiansah Sujaya, Dedi Herdiansah Dendi Hidayatullah, Dendi DIAH AYU SATYARI UTAMI, DIAH AYU SATYARI Dinamella Wahjuningrum Eddy Supriyono Enang Harris Enang Harris Surawidjaja, Enang Harris Esti Handayani Hardi Fachriyan Hasmi Pasaribu Firdausi, Amalia Putri Gustilatov, Muhamad Henky Manoppo Ince Ayu Khairana Kadriah Jasmanindar, Yudiana Junior, Muhamad Zairin Kadriah, Ince Ayu Khairani Komar Sumantadinata Kukuh Nirmala Listiyowati, Nunuk Mia Setiawati Mochamad Fatuchri Sukadi Muchtar, Muthahharah Muh. Aris Muh. Fatuhcri Sukadi Muhammad Yamin MUHAMMAD ZAIRIN JUNIOR Mulyani, Rahma Munti Yuhana Nafiqoh, Nunak Nainggolan, Ronald Kriston Sauttua Nissa, Khairun Nugrahawati, Anis Nur Bambang Priyo Utomo ODANG CARMAN Priadi Setyawan, Priadi Puadi, Evan Farhan Wahyu Rahman Rahman Rajab, Fahmi Ramadhani, Dian Eka Retno Damayanti Soejoedono Ridwan Affandi Rudi, Mad Sariati, Woro Nur Endang Septiani, Ghita Ryan Setyawan, Agung Cahyo Siti Subaidah Sri Nuryati Sugiani, Desy Susianingsih, Endang Susianingsih, Endang Tatag Budiardi Tepu, I. Tito Hardiyanto, Tito Tuti Sumiati, Tuti Umbara, Uba Utomo, Nur Bambang Priyo Wesly Pasaribu Widanarni Widanarni Wiyoto Wiyoto, Wiyoto Yani Hadiroseyani Yosmaniar, Yosmaniar Yumaidawati, Nurfitriani Siti Zahra, Aminatul Zairin Jr., Muhammad