Yuhara Sukra
Bagian Anatomi, Histologi, dan Embriologi, Departemen Anatomi, Fisiologi, dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor

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PRODUKSI EMBRIO KUCING SECARA IN VITRO DARI SPERMATOZOA HASIL PRESERVASI MELALUI FERTILISASI MIKRO Eriani, Kartini; Sukra, Yuhara; Boediono, Arief; Djuwita, Ita; Sumarsono, Sony Heru
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.563

Abstract

Penelitian ini bertujuan mengetahui motilitas dan kemampuan memfertilisasi sperma kucing yang berasal dari epididimis yang disimpan pada suhu 4° C. Epididimis disimpan dalam media phosphate buffer saline (PBS) pada suhu 4? C selama 1, 3, dan 6 hari. Viabilitas spermatozoa diamati dengan pewarnaan hoechst-propidium iodine. Fungsi biologis spermatozoa dievaluasi melalui teknik kultur in vitro dengan fertilisasi mikro dan perkembangan embrio di dalam media kultur CR1aa. Hasil penelitian menunjukkan persentase spermatozoa hidup pada hari ke-1, 3, dan 6 penyimpanan masing-masing adalah 81,0; 71,7; dan 70,7% (duktus deferens), 84,0; 81,2; dan 63,2% (kauda epididimis), 84,0%; 75,0; dan 74,7% (korpus epididimis). Persentase pronukleus (PN) yang didapat dengan teknik intra cytoplasmic sperm injection (ICSI) menggunakan spermatozoa epididimis pada hari ke-1, 3, dan 6 hari penyimpanan masing-masing adalah 8,0; 10,0; dan 5,9%. Preservasi epididimis pada suhu 4? C dalam PBS dapat digunakan untuk fertilisasi dan menghasilkan embrio kucing secara in vitro.
THE INFLUENCE OF HOMOLOGOUS AND HETEROLOGOUS SERA IN CULTURE MEDIUM ON IN VITRO MATURATION AND FERTILIZATION OF SHEEP OOCYTES Djuwita, Ita; Rusyiyantono, Yohan; Muhamad, Kusdiantoro; Purwantara, Bambang; Sukra, Yuhara
Media Veteriner Vol. 5 No. 1 (1998): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.792 KB)

Abstract

The influence of various types of sera i.e. Fetal Calf Serum (FCS), Bovine Post Estrous Serum (SPES) as heterolog sera; and Ewes Fetal Serum (EFS) and Ewes Serum (ES) as homologue sera on in vitro maturation and fertilization of sheep oocytes was investigated using Tissue Culture Medium 199TM (TCM 199. Gibco). Sovine sera were collected from cow at seven days post-estrus, while ewes sera were collected during estrous (ES-HO) and six days post estrous (ES-H6). The result shows that the maturation rate of oocytes cultured in medium supplemented with EFS, ES-HO and ES-H6 at  concentrations of 32.9%, 68.7%, and 67.6% respectively is better than those supplemented with FCS and SPES at concentrations of 22.2% and 28.9%, respectively. In vitro fertilization rate were significantly higher in medium supplemented with ES-HO and ES-H6 at concentration of 65.4% and 65.8% (P < 0.05) than that supplemented with SPES at concentration of 31.7%. In conclusion, ewes sera could be used in culture medium for promoting both in vitro maturation and fertilization rate of sheep oocytes.
THE EFFECT OF SUBLETHAL CONCENTRATION OF PHOSPHAMIDON ON TISSUE DAMAGE OF NILE FISH (OREOCHROMIS NILOTICLIS TREW.) Efrizal, T; Setijanto, Heru; Lumbanbatu, Djamar Tumpal F.; Sukra, Yuhara
Media Veteriner Vol. 5 No. 4 (1998): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (997.932 KB)

Abstract

The effect of sublethal concentrations of phosphamidon on tissue damage of nile fish (Oreochromis niloticus Trew.) were studied at the Laboratory of Aquatic Animal Physiology Faculty of Fisheries and at the Laboratory of Anatomy F aculty of Veterinary Medicine Institut Pertanian Bogor. Renewal test method with 40 % of the media renewed everyday was used for the examination of the sublethal toxicity. Each test consisted of 30 nile fish in 15 1 water. The concentrations of phosphamidon used in this study was 0 mg/l (control group), 3.7 mg/l, 7.4 mg/l, dan 11.0 mg/l, respectively, and all groups were repeated three times. Histopathologically, the gill yielded secondary lamella damages, necrosis, hipertrophy, fusion lamella and degeneration of cartilaginous supportive tissue. The intestine showed necrosis of mucous cells, hipertrophy, bleeding in the lamina propria, and proliferation of connective tissue. In the hepatopancreas organ there were bleeding, infiltration of leucocytes and proliferation of connective tissue.
THE EXPERIMENTS WERE CARRIED OUT TO STUDY THE INFLUENCE OF DIFFERENT SERA ON IN VITRO MATURATION AND EARLY DEVELOPMENT OF OVINE EMBRYOS. SERA USED IN THIS STUDY WERE FLS (FETAL LAMB SERUM), EWE SERUM COLLECTED ON DAY 0 (ES-DO) AND DAY 6 (ES-D6) OF OESTRUS CYCLES. OVINE OOCYTE WERE MATURED AND CULTURED IN TCM-199 SUPPLEMENTED WITH 10 % OF DIFFERENT SERA. RESULTS OF THIS EXPERIMENT SHOWED THAT SUPPLEMENTATION OF ES-DO OR ES-D6 COULD SUPPORT MATURATION RATE (METAPHASE-II) AT 68.7% AND 67.6%, RESPEC Rusiyanto, Yohan; Purwantara, Bambang; Sukra, Yuhara
Media Veteriner Vol. 7 No. 1 (2000): Media Veteriner
Publisher : Media Veteriner

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Abstract

The experiments were carried out to study the influence of different sera on in vitro maturation and early development of ovine embryos. Sera used in this study were FLS (Fetal Lamb Serum), ewe serum collected on Day 0 (ES-DO) and Day 6 (ES-D6) of oestrus cycles. Ovine oocyte were matured and cultured in TCM-199 supplemented with 10 % of different sera. Results of this experiment showed that supplementation of ES-DO or ES-D6 could support maturation rate (Metaphase-II) at 68.7% and 67.6%, respectively better than FLS (32.9%). The fertilization rate was significantly higher (p
RESPONSES TO DIFFERENT DOSES OF PMSG ON EMBRYO COLLECTION IN RATS Poernomo, Bambang; Sukra, Yuhara
Hemera Zoa Vol. 75 No. 2 (1992): Jurnal Hemera Zoa
Publisher : Hemera Zoa

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Abstract

The development ability of embryos recovered from LMR - Strain rats (age 3 to 4 months with an average 120 g). embryos recovered from treated animals with 10 and 20 IU PMSG, respectively, were compared with those from control (0 IU). Embryos were collected from uteri on day 4 post coital.Treated groups tended to show an increase willingness to mate comfored to control groups, but a high number of embryos that have degenerated in rats treated with 20 IU PMSG were observed. The experiment suggested, that the dose of PMSG was an important source of variation in embryo production.