C Sumantri
Fakultas Peternakan Institut Pertanian Bogor

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GHRH|HAEIII GENE POLYMORPHISM IN DAIRY AND BEEF CATTLE AT NATIONAL LIVESTOCK BREEDING CENTERS Rini, A O; Sumantri, C; Anggraini, A
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1164.214 KB) | DOI: 10.5398/medpet.2013.36.3.185

Abstract

This study was aimed to identify polymorphism of growth hormone releasing hormone (GHRH) gene in 89 heads of Holstein-Friesian (HF) dairy cattle from Lembang Artificial Insemination Center/LAIC (17 bulls), Singosari Artificial Insemination Center/SAIC (32 bulls), and Cipelang Livestock Embryo Center/CLEC (40 cows); as well as in 4 breeds of female beef cattle from CLEC for comparison, providing Simmental (13 cows), Limousin (14 cows), Brahman (5 cows), and Angus (5 cows). This study used PCR-RFLP method by using HaeIII restriction enzyme. The enzyme cut the GHRH gene at nucleotides of GG|CC at the base positions of 118, 312, and 406 and produced 4 fragments of 118, 194, 94, and 45 bp respectively.  Genotyping the GHRH gene produced two types of allele, namely A (312, 94, and 45 bp) and B (194, 118, 94, and 45 bp). These two alleles resulted in three types of genotype, namely AA (312, 94, and 45 bp), AB (312, 194, 118, 94, and 45 bp), and BB (194, 118, 94, and 45 bp).  Frequency of the B allele was dominant to the A allele. Chi-Square analysis showed that all of HF dairy and beef cattle observed were in Hardy-Weinberg equilibrium (X2 < X2(0.05)). The highest heterozygosity value was 0.471 for HF bulls in LAIC, while the lowest one was for HF bulls in SAIC. Heterozygosity values in Simmental and Limousin cattles were higher than that of in HF cattle. The GHRH gene in HF and beef cattle was polymorphic, the exception was for Brahman with the only B allele. This result will improve the understanding of the polymorphism of GHRH gene in dairy and beef cattle.
ESTIMASI JARAK GENETIK ANTAR DOMBA GARUT TIPE TANGKAS DENGAN TIPE PEDAGING Mansjoer, S S; Kertanugraha, T; Sumantri, C
Media Peternakan Vol. 30 No. 2 (2007): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

The objectives of the present study were to estimate the genetic distance and to determine the discriminant variables among fighting and meat types sheep of Garut (Garut sheep). A total of 413 heads of Garut sheep from five sub-populations were used in this study. Data were collected from the Regional Animal Research Center (BPPTD) as follows: 102 heads of common type sheep from Margawati (M), 69 heads from meat type sheep from Wanaraja (P), 72 heads of meat type from Sukawening (D), 81 heads of fighting sheep from Wanaraja (T) and 89 heads of fighting sheep from Sukawening (A). Data obtained were analyzed by simple discriminant with SAS package program version 7.0 and MEGA2 program to get the construction of phenogram tree. The results showed that in general fighting sheep had body weights and body measurements significantly higher (P < 0.05) than meat type sheep. However they were almost the same as common type sheep Margawati. The length and width of ears, tail width and chest width were the most discriminant variables to determine the differences of fighting type and meat type sheep. The highest similarities between individuals inside the group were obtained from Margawati common type sheep (71.57%), followed by Wanaraja meat type sheep (69.57%), Sukawening meat type sheep (69.44%), Wanaraja fighting type sheep (69.14%) and Sukawening fighting type sheep (59.55%). The Wanaraja fighting type sheep (T) had the closest genetic distance (1.16) to the Sukawening fighting type sheep (A), while the longest genetic distance (6.17) was between the Wanaraja meat type sheep and the Margawati common type sheep. The phenogram tree showed that the Margawati common type sheep was outside Wanaraja and Sukawening fighting type and meat type sheep, but the genetic distances were closer for the Wanaraja and Sukawening fighting type sheep. Key words: Garut sheep, genetic distance, body measurements
IDENTIFIKASI GEN MX1 (GEN RESISTEN TERHADAP VIRUS INFLUENSA) PADA PBAC PUSTAKA GENOM BABI Sumantri, C; Morozumi, T; Hamashima, N
Media Peternakan Vol. 24 No. 2 (2001): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Identifikasi Gen MX1 (Gen Resisten terhadap Virus Influensa) pada pBAC Pustaka Genom Babi
PEMBENTUKAN BLASTOSIT SECARA FERTILISASI IN VITRO PADA SAPI DARI BANGSA DAN KONSENTRASI SPERMA YANG BERBEDA Sugiono, Sugiono; Wijayanto, T; Sumantri, C
Media Peternakan Vol. 24 No. 3 (2001): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Pembentukan Blastosit Secara Fertilisasi In Vitro pada Sapi dari Bangsa dan Konsentrasi Sperma yang Berbeda
IDENTIFICATION OF SINGLE NUCLEOTIDE POLYMORPHISM ON GROWTH HORMONE GENE IN ACEH CATTLE Sari, E M; Noor, R R; Sumantri, C; Yunus, M; L, Han J; Muladno, .
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (492.152 KB) | DOI: 10.5398/medpet.2013.36.1.21

Abstract

This research was aimed to identify the changes of nucleotide (Single Nucleotide Polymorphism) growth hormone gene in the population of Aceh cattle. There were 44 samples of DNA sequenced, and a few samples from Gen Bank (M57764). Based on the analysis using MEGA program, it was identified one new mutation on exon five on 2230 bp in which C nucleotide turned into T nucleotide, and this was called Silent Mutation (Leusine-Leusine/ CTC-CTT). The frequency of Single Nucleotide Polymorphism (SNP) genotype on 2230 bp (C/T) was CC (0.36), TT (0.14) and CT (0.50). The genotype TT was not possessed by Aceh cattle from Saree, but possessed by those from Banda Aceh and Indrapuri. Chi-square test showed not significant differences in allele frequencies for three population. The frequency of genotype SNP on 2291 bp (A/C) was AC (0.11) and CC (0.89). The frequency of allele C was higher than allele A and T.
PENDUGAAN JARAK GENETIK PADA ITIK CIHATEUP, CIREBON DAN MOJOSARI Muzani, A; Brahmantiyo, B; Sumantri, C; Tapyadi, A
Media Peternakan Vol. 28 No. 3 (2005): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

A study on morphological body conformation of Cihateup, Cirebon and Mojosari ducks was carried out to determine the genetic distance and discriminant variables. The research was done at PT Dwi Mitra Daq, Gunung Sindur, Bogor, using 30 Cihateup, 30 Cirebon and 30 Mojosari ducks, from September to November 2004. Length of neck, wing, circumference of chest, deep chest, body weight, circumference of tarsometatarsus, length of chest, third digit, tarsometatarsus, tibia, femur and maxilla were measured to observe their body sizes to determine the genetic distance and discriminant variables. Data obtained were analyzed by using GLM (General Linear Model) and simple discriminant analysis with SAS package program version 6.12 and program MEGA2 to make the construction of tree fenograms. Cihateup ducks had larger body size (i.e. length of neck, wing, circumference of chest, deep chest, length of third digit and femur) than Cirebon and Mojosari ducks, but were smaller on length of tarsometatarsus than Mojosari ducks. Results from canonical analysis showed that the most discriminant variables were obtained from the length of third digit tarsometatarsus, tibia and femur. Cihateup ducks were mixed with Cirebon ducks (36,67%), Cirebon ducks were mixed with Cihateup ducks (43,33%) and Mojosari ducks (3,33%), Mojosari ducks were mixed with Cihateup ducks (3,33%) and Cirebon ducks (13,33%). Mahalanobis genetic distance showed that Cihateup and Cirebon ducks had a genetic distance of 0,880, Cihateup and Mojosari, and, Cirebon and Mojosari had 1,786. Key words : duck, morphologycal, genetic distance, body conformation
DISTRIBUSI DAN FREKUENSI GEN KEJADIAN DELESI DAN MUTASI PADA EXON-14 GEN MX1 (GEN RESISTEN TERHADAP VIRUS INFLUENSA) PADA BERBAGAI BANGSA BABI Sumantri, C
Media Peternakan Vol. 24 No. 2 (2001): Media Peternakan (Supplement Edition: Animal Production)
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Abstract

Distribusi dan frekuensi gen kejadian delesi dan mutasi pada exon-14 gen MX1 (Gen resisten terhadap virus influensa) pada berbagai bangsa babi
ANALISIS HUBUNGAN KERAGAMAN GEN SCD1 (STEAROYL COA DESATURASE) DENGAN KOMPOSISI ASAM LEMAK DAGING SAPI LOKAL DI CIAMIS Hilmia, Nena; Noor, R.R; Sumantri, C; Priyanto, R; E, Gurnadi
Jurnal Ilmu Ternak Universitas Padjadjaran Vol 16, No 2 (2016)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v16i2.11573

Abstract

SCD1 merupakan kandidat gen yang berperan dalam mengkonversi asam lemak jenuhmenjadi  tidak  jenuh,  dan  rasio  keduanya  menentukan  kualitas  daging.  Single  NucleotidePolymorphism (SNP) V293A pada gen SCD1 yang   mengubah asam amino Valina menjadiAlaninaberpengaruh terhadap komposisi asam lemak daging. Penelitian ini bertujuan mengidentifikasi keragaman gen SCD1 berdasarkan SNP V293A, dan hubungannya dengan kandungan  asam lemak pada sapi lokal  di Ciamis. Penelitian ini menggunakan 14  sampel daging  dan  DNA  dengan  genotipe  yang  berbeda.  Metode  PCR-RFLPdengan  enzim  Aci1digunakan untuk mendeteksi SNP V293A pada exon 5.   Hubungan keragaman gen SCD1 dengan komposisi asam lemak daging dianalisis dengan uji T. Hasil penelitian ini menunjukkan gen SCD1 pada sapi lokal Ciamis polimorfik, terdapat dua alel, yaitu alel T dan C dengan tiga genotipe,   yaitu TT, CT  dan CC.  SNP V293A pada gen SCD1 tidak  berpengaruh terhadap komposisi asam lemak daging sapi lokal di Ciamis Jawa Barat.Kata kunci : asam lemak daging, PCR-RFLP, Sapi lokal Ciamis, SCD1.
GROWTH HORMONE GENE POLYMORPHISM AND ITS ASSOCIATION WITH PARTIAL CUMULATIVE MILK YIELDS OF HOLSTEIN FRIESIAN DAIRY CATTLE Misrianti, R; Anggraeni, A; Andreas, E; Sumantri, C
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.573 KB) | DOI: 10.5398/medpet.2012.35.3.145

Abstract

Growth hormone gene (GH gene) plays an important role in regulating body growth and in developing mammary gland, similar with its interaction to specific receptors. The GH gene has been considered as one of candidate gene associated with selection on lactation trait and milk production. This study was aimed to determine genetic polymorphism of the GH-AluI gene and to associate its genotype variants on various 15-d partial cumulative milk yields in Holstein Friesian (HF) dairy cows. A number of 370 blood samples were collected from six HF populations, respectively from small dairy farmer under the supervision of the North Bandung Milk Cooperation (NBMC) in Cilumber (98) and Pasir Kemis village (96), Dairy Cattle Breeding and Improvement Station (Cikole DCBIS) Cikole (88), Lembang Artificial Insemination Center (Lembang AIC) (17), Singosari Artificial Insemination Center (Singosari AIC (32), and Cipelang Livestock Embryo Center (Cipelang LEC) (40). A polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method was used to identify variant genotypes of the GH gene using AluI restriction enzyme. Genotyping results produced only two genotypes, i.e. LL and LV genotypes, without VV genotype. Frequency of the former was dominant, whilst that was low for the latter (89% vs. 11%); leading to the frequency of L allele was very high (94%) compared to that of V allele (6%). No significant association between variant genotypes (LL and LV) and various 15-d partial cumulative milk yields.
Performances and phylogenic relationships among local sheep in Indonesia by morphological analysis Sumantri, C; Einstiana, A; Salamena, J.F; Inounu, I
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.297 KB) | DOI: 10.14334/jitv.v12i1.563

Abstract

The morphological discriminant and canonical analysis were carried out to estimate the phylogenic relationship and determine the discriminant variables between Indonesian local sheep of thin tail sheep from Jonggol (Bogor) and Garut and fat tail sheep from Indramayu (originated from East Java), Donggala, Madura, Kisar, Rote and Sumbawa. The number of sheep used  was totally 818 heads collected from 8 populations, were Jonggol (185), Garut (74), Indramayu (100), Donggala (60), Madura (86) Kisar (231), Rote (52), and Sumbawa (30). Discriminant analysis used for body weight and body measurements were body length, wither height, chest width, chest depth, chest circumference, skull length, skull width, skull height, tail lenght, tail width, ear length and ear width. SAS package program was used to analyze the data. The results from analysis variant showed that the body weight and body measurenment of Garut sheep almost the same with sheep from Indramayu and significantly higher (P&lt;0,05) compared to another breeds. Whereas, Kisar and Rote have body weight and body measurenment significantly lower (P&lt;0,05) compared to another breeds. The results from Mahalanobis distance (phenogram tree) and canonical analyses showed that breeds were divided into five groups, the first group is Garut, the second is thin-tail sheep which existed in Jonggol, the third was fat-tailed sheep which existed in Kisar, Rote, and Sumbawa, the fourth is fat-tail sheep which existed in Donggala, and the fiveth was fat-tailed sheep which existed in Indramayu and Madura. The groups of sheep between Kisar and Rote had the smallest genetic distance value equal to 1,623 and groups between Donggala and Garut had the biggest genetic distance value equal to 7,994. The highest similarity between individual inside the group was obtained from Sumbawa (100%) and the lowest similarity was from Rote (72,41%). The results from canonical analyses showed that tail lenght, ear width, ear lenght, skull lenght, body lenght, and tail width were the most discriminant variables to determine the diferences between breeds. Key Words: Sheep, Genetic Distance, Analysis Discriminant and Cannonica