C. SUMANTRI
Departemen Ilmu Produksi dan Teknologi Peternakan, Institut Pertanian Bogor

Published : 55 Documents
Articles

PENDUGAAN BOBOT BADAN MELALUI ANALISIS MORFOMETRIK DENGAN PENDEKATAN REGRESI TERBAIK BEST - SUBSET PADA DOMBA GARUT TIPE PEDAGING, TANGKAS DAN PERSILANGANNYA GUNAWAN, A.; JAMAL, K.; SUMANTRI, C.
Majalah Ilmiah Peternakan Vol 11 No 1 (2008)
Publisher : Fakultas Peternakan Universitas Udayana

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Abstract

ABSTRACT ESTIMATE OF BODY WEIGHT FIGHTING AND MEAT GARUT SHEEP AND CROSSBREED WITH MERFOMETRIC ANALYSIS APPROACH The aims of this study were to estimate body weight and to determine the best equation which can be used to estimate body weight of fighting and meat Garut sheep and its crossbreed type at district and outdistrict Garut. This study used primary data of Garut sheep from Garut district consisting of Margawati, Wanaraja and Sukawening. For outdistrict Garut, it was used Garut sheep from Bogor consisting of Ciomas and Cinagara. The total number of sheep collected were 531 heads. The differences of body measurements locations were analyzed by t-test, Correlation, and Regression Multiple Linear Regression and Polynomial Regression (Linear, Quadratic, and Cubic) Analyses. The results showed that body weight of fighting and meat rams and ewes from Wanaraja were the highset than another Garut sheep and crossbreed. The highest body measurements were found from Wanaraja (fighting and meat type) and Sukawening (fighting type) sheep and the lowest body measurements were found from Margawati and Cinagara. Whilst that some body measurements of rams were not different (P>0.05). Correlation coefficient between body weight and various body measurements body lenght, weither height, chest deep and chest circumference of Garut Sheep were highest at district and outdistrict of Garut. The best regression equations to describe the relation ship between body weight and body measurements for rams and ewes Garut sheep at district and outdistrict Garut were multiple regression equations. The highest determination coefficient (100%) was shown for meat type from Wanaraja and the lowest one (93.93%) was shown of fighting type from Sukawening. ABSTRAK Penelitian ini bertujuan untuk menduga bobot badan dan mencari model terbaik dalam menduga bobot badan domba Garut tangkas, pedaging dan persilangannya di daerah Garut dan luar Garut. Total domba yang digunakan pada penelitian ini sebanyak 531 ekor domba Garut dari beberapa lokasi di kabupaten Garut (Margawati, Wanaraja, Sukawening) dan di Luar Garut (Ciomas dan Cinagara). Data dari hasil penelitian dianalisis dengan menggunakan Uji-t, Korelasi, dan Regresi meliputi Analisis Regresi Linier Ganda dan Analisis Regresi Polinomial (Linier, Kuadratik, dan Kubik). Hasil Uji-t menunjukkan, bahwa rataan bobot badan domba tangkas Garut baik jantan maupun betina dari Wanaraja memiliki bobot badan paling besar dibandingkan dengan domba Garut lain dan persilangannya. Ukuran tubuh tertinggi pada domba Garut ditunjukkan pada domba tangkas dan pedaging Wanaraja serta tangkas Sukawening dan ukuran tubuh terkecil ditunjukkan pada domba Garut di Margawati dan Cinagara. Koefisen Korelasi tertinggi antara bobot badan dan ukuran-ukuran tubuh pada domba Garut tangkas, pedaging da persilanganya ditunjukkan antara bobot badan dengan panjang badan, tinggi pundak, dalam dada dan lingkar dada. Persamaan regresi yang paling baik digunakan menduga bobot badan berdasarkan ukuran-ukuran tubuh pada domba Garut tangkas, pedaging dan persilangannya adalah Persamaan Regresi Linier Ganda dengan tingkat akurasi berkisar antara 93,3%-100%. Tingkat akuras tertinggi ditunjukkan pada domba tangkas Wanaraja dengan tingkat akurasi 100% dan terendah ditunjukkan pada domba tangkas di Sukawening dengan tingkat akurasi 93,93%.
Novel variant in the leptin receptor (LEPR) gene and its association with fat quality, odour and flavour in sheep Gunawan, A.; Pramukti, F. W.; Listyarini, K.; Abuzahra, M. A.; Jakaria, J.; Sumantri, C.; Inounu, I.; Uddin, M. J.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

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The aim of this study was to investigate the association and expression of LEPR with fat quality, flavour and odour in sheep. A total of 47 rams were used in this study. Fatty acids composition, BCFA (Branch Chanin Fatty Acids; MNA, MP, MOA, and EOA) and skatole (MI) representing fat quality, flavour and odour in sheep were measured at ages of 10-12 months old. Identification of novel variant and expression analysis of LEPR gene with different fat quality, odour and flavour were performed by PCR RFLP and qRT-PCR, respectively. A SNP in genomic region g.40854778 A>C of the LEPR gene was associated (P<0.05) with fat quality of saturated fatty acids (SFA) including [tricosanoic acid (C23:0) and tetracosanoic acid (C24:0)] and poly unsaturated fatty acid (PUFA) [docosahexaenoic acid (C22:6n3)]. However, the novel variant of LEPR was not significantly associated (P<0.05) with fat content, odour and flavour (MNA, MP, MOA, EOA, and MI). The mRNA expression analysis showed that LEPR mRNA expression was higher (P<0.01) in sheep found at the AA genotype. It could be concluded that variant SNP in the LEPR may contribute to fatty acid composition without influencing odour and flavour traits in sheep.
ANALYSIS OF PROLACTIN GENE EXON 4 DIVERSITY IN PEKING, WHITE MOJOSARI, AND PEKING WHITE MOJOSARI CROSSBREED Indriati, M.; Sumantri, C.; Susanti, T.
Media Peternakan Vol. 39 No. 1 (2016): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.476 KB) | DOI: 10.5398/medpet.2016.39.1.14

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Genetic marker linked to loci reproductive traits could be used to increase an effectiveness of improvement in animal breeding. Association between DNA polymorphism and a trait could be considered as candidate genetic marker for marker assisted selection (MAS) programs. Prolactin (PRL) is one of polypeptide hormones secreted by anterior pituitary gland in vertebrates. PRL plays an important role in onset of poultry incubation and brooding behavior. The aim of this study was to investigate the diversity of prolactin gene and to characterize the type of mutation in partial intron 3, intron 4 and exon 4 of duck prolactin gene. Blood extraction was collected from 168 ducks consisted of 19 Peking, 36 Mojosari, and 113 Peking White Mojosari (Peking Mojosari putih) ducks. Polymerase chain reaction of fragment prolactin gene exon 4 and partial intron 3 and 4 have been successfully amplified with length of base pair were 496 bp. A total of 30 µL PCR product from each sample were sequenced for forward sequence using BIOTRACE 3730 by First Base Company, Malaysia. Alignment analysis found six SNP consisted of g.3941T&gt;G, g.3975C&gt;A, g.4110T&gt;C, INDEL 3724A,  INDEL 34031, and INDEL 3939A. Analysis of SNP frequency result indicated mutation of INDEL 3724A, g.3941T&gt;G, g.3975C&gt;A, INDEL 4031A and g.4110T&gt;A in duck sample were polymorphic and INDEL 3939A were monomorphic.
Polymorphism of stearoyl-CoA desaturase (SCD1) gene in Indonesian local cattle Hilmia, N.; Noor, R. R.; Sumantri, C.; Gurnadi, R. E.; Priyanto, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 1 (2017): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.456 KB) | DOI: 10.14710/jitaa.42.1.1-5

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Stearoyl-Coa desaturase (SCD1) gene is one of genes that involves in converting saturated fatty acids to unsaturated fatty acids. SNP at exon 5 in SCD1 gene that changes amino acid valine to alanine (V293A) has an influence to meat fatty acid composition. The aim of this research was to analyze SCD1 gene polymorphisms based on SNP V293A at exon 5 of three Indonesian local cattle. The identification of SCD1 gene polymorphisms was done by using 98, 20 and 7 DNA sample from Ciamis, Bali/Banteng, and Ongole Grade (PO) cattle, respectively. PCR_RFLP method with AciI enzim was carried out to identify SNP Val293Ala. Allelic frequencies and heterozygosity value were analyzed by using POPGENE32. The result showed that SCD1 gene at Ciamis local cattle and PO cattle were polymorphic. Their frequencies were 74.5% and 71.4% for T and 25.5% and 28.6% for C, respectively. There were three genotypes on Ciamis local cattle i.e TT, CT and CC with their frequencies were 52%, 44.9% and 3.10%, respectively. There were two genotypes on PO cattle i.e TT and CT with their frequencies were 42.9% and 57.1%, respectively. Meanwhile, SCD1 gene in Bali cattle was monomorphic. Heterozygosity value of SCD1 gene in Ciamis and PO cattle were 0.38 and 0.44, respectively. Their heterozigosities were categorized as medium. 
Peningkatan produktifitas lebah madu melalui penerapan sistem integrasi dengan kebun kopi Saepudin, R.; Fuah, A. Fuah M.; Sumantri, C.; Abdullah, L.; Hadisoesilo, S.
Jurnal Ilmu-Ilmu Peternakan Vol 21, No 1 (2011)
Publisher : Fakultas Peternakan Universitas Brawijaya

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Abstract : The study of relationship between the honey productivity and honey bee-coffee plantation integration was conducted in Kepahiang, the Province of Bengkulu. The objective of this study was to evaluate the application of Apis cerana-coffee plant integration system on honey production and coffee bean as well.. The experiment was arranged in a completely randomized design with two treatments and ten replications. The result showed that honey production was higher by 114% than that outside the plantation. Similar to the honey productionn, coffee been production at honeybee-coffee plantation integration was significantly higher by 10,55 % than that was unpollinated by Apis cerana.Key words: cerana, coffee, integration. production
Verification for the control of kappa-casein gene on milk protein yield of friesian-holstein in dairy central region of West Java Anggraeni, A.; Sumantri, C.; Farajallah, A.; Andreas, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.262 KB) | DOI: 10.14334/jitv.v14i2.354

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Genotipe of BB kappa kasein (ĸ-kasein) gene positively influences high milk protein yield. The aim of this research was to verify the use of the к-casein gene as marker of assisted selecion (MAS) of high protein yield in domestic Holstein-Friesian (HF) cattle. Genotyping blood samples was conducted for lactating cows from BPPT-SP Cikole (82 head) and a number of small holders in KPSBU Lembang (190 head). Genotyping the к-casein gene was also conducted for active and non active AI bulls (HF) from BIB Lembang (25 head) and BBIB Singosari (32 head). Varian genotipes were identified trough DNA migration, whereas frequencies of alleles were calculated by Nei Method (1987). The effects of variant genotypes on protein yield and other milk component yields were studied in lactating cows (56 head in BPPT-SP Cikole and 111 head in KPSBU Lembang) by applying Least Square Means (LSM) method of the General Linear Model (GLM). Genotyping the ĸ-kasein gene resulted in three genotypes, namely AA, AB and BB, with two alleles, namely A and B. It was identified that a very limited number of lactating cows with BB genotype (0-9%). These cows produced higher protein yield around 3.37-3.84% than that of AA cows, while AB cows produced protein yield in between, though these differences were not statistically significant (P&gt;0,05). In contrast, AA cows tended to produced higher milk fat yield than BB cows. Cows BB and AB seemingly produced higher dry matter, while both specific density and pH were not affected by variant genotypes of the к-casein gene.  The results showed that BB genotype of the к-casein gene tended to be quitely consistent in controlling high protein yield, so it would be a good oppurtinity to be used as MAS of milk protein yield in HF domestic. Key words: Protein Yield, Dairy Cattle (HF), к-Casein Gene, Genotype BB
Polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) Gene at Locus (Pit-1-Hinf1) and its effects on dam body weight and milk production of local sheeps Sumantri, C.; Herdiana, D.; Farajallah, A.; Rahmat, D.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.36 KB) | DOI: 10.14334/jitv.v14i3.344

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Pituitary-Specific Transcription Factor-1 (Pit-1) is a transcription factor with critical role in the transcriptional regulation of multiple genes in the pituitary. The objective of this research was to identify polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) gene at Locus (Pit-1-Hinf1) and to investigate any possible associations of Pit-1 genotypes on dam body weight, milk production and milk quality in local sheep at the Jonggol Animal Science Teaching and Research Unit (JASTRU), Fact. Anim. Sci. Bogor. Agric. Univ. A total number of 161 blood samples were collected from 3 local sheep, namely Garut from Wanaraja (55 hd), Garut from Margawati (23 hd) and lactating ewes (83 hd) from JASTRU  farm in Bogor. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products were digested with a Hinf1 enzyme restriction. Fragments of Pit-1gene at locus Pit-1-Hinf1 was detected by a silver-staining method. A length of 637 base pairs (bps) of the Pit-1 gene of local sheep was successfully amplified. The Hinf1 restriction enzyme cut the PCR product into three different length of fragments succesively at 345, 137, and 115   bps designated as A allele; whilst B allele had four fragments at 283, 137, 115, and 62 bps respectively. The locus of Pit-1-Hinf1 was polymorphic in local sheep from Jonggol, however it was monomorfic in Garut sheep. The frequencies of A and B alleles were 0,806 and 0,194 respectively. Pit-1 genotypes had no significant effect on dam body weight and milk production. This result is indicating that the use of single locus Pit-1-Hinf1 in Pit-1 gen is less effective to be used as a candidate in selecting dam body weight and milk production in these three local sheep. Key words: Local Sheep, Pit-1 Gene, Polymorphism, Dam, Milk
κ-CASEIN GENE POLYMORPHISMS IN RIVERINE AND SWAMP BUFFALO IN INDONESIA Rini, A.O.; Sumantri, C.; Damayanthi, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 1 (2014): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.492 KB) | DOI: 10.14710/jitaa.39.1.1-9

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Kappa-casein (?-casein) gene is known as a gene that plays a role in controlling milk protein andalso play a crucial role in the coagulation and curdling of milk. This study was aimed to identifypolymorphisms of the ?-Casein gene of local buffaloes in Indonesia. A total number of 40 heads ofriverine buffalo and 250 heads of swamp buffalo. This study used PCR-RFLP method, whichamplification of the ?-Casein gene resulted an amplicon with length of 157 bp, located in exon 4. Theamplified fragment were digested with EcoRV restriction enzyme, which cut the ?-Casein gene in exon4 at nucleotides of GAT|ATC, revealed the presence of one polymorphism at the base position of 23 bpthat occurs with a substitution of Ile (ATC) of the T genetic variant into Thr (ACC) of the C geneticvariant. Genotyping ?-Casein gene in riverine buffalo produced two types of allele, namely C allele (157bp) and T allele (136 and 21 bp). These two alleles resulted in three types of genotypes, namely CC, CT,and TT. Frequency of the C allele was dominant to T allele. ?-Casein gene in swamp buffalo wasmonomorphic with one allele, namely C allele. Heterozygosity value of riverine and swamp buffaloeswere low. PIC value in riverine and swamp buffalo ranged 0.000-0.288. Fixation index of ?-Casein genein riverine buffalo was low (Siborong-borong SBBC = -0,0036; Deli Serdang = -0.025), but in swampbuffalo was in fixation. This study showed that ?-Casein|EcoRV were polymorphic in riverine buffaloand monomorphic in swamp buffalo.
PRODUCTIVITY AND GENETIC DIVERSITY OF LOCAL CATTLE IN CIAMIS-WEST JAVA Hilmia, N.; Noor, R.R.; Sumantri, C.; Gurnadi, R.E.; Priyanto, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 1 (2013): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.858 KB) | DOI: 10.14710/jitaa.38.1.10-19

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The objectives of this study were to identify the productivity and genetic diversity of local cattle inCiamis West Java based on DNA microsatellite, in order to provide the basic information for its rearing,conservation and development. Eighteen of local cattle were kept for 58 days by feeding concentratesand rice straw. The measured parameters were weekly body weight as well as carcass percentage. Thepercentage of comparing carcass was taken from PO, Bali and crossbred cattle from local cattle withLimousin, Simmental, Brahman and Angus cattle. Data were analyzed using Excel software and SAS(2004). Blood samples were taken from two subpopulations i.e., 46 and 52 samples from Tambaksariand Cijulang areas, respectively. Other DNA samples were collected from Bali, PO, Limousin, Simental,Brahman and Angus cattle. Genetic diversity and phylogenetic analysis were analyzed using 3microsatellite loci, that were INRA35, HEL9 and BM2113. The genotyping data were analyzed usingPOPGENE.32 program, while phylogenetic trees were analyzed by MEGA 4. Average daily gain (ADG)of local cattle in Ciamis was 0.62±0.23 kg/head/day with a carcass percentage was 51.62±1.80%. Thecarcasses percentage of local cattle in Ciamis was not significantly different from those of Bali, PO, andcrossbred cattle. The genetic diversity of local cattle in Ciamis was categorized in the medium level, thediversity of local cattle in Tambaksari subpopulation was higher than in Cijulang subpopulation. Localcattle in Ciamis had a closer genetic distance with the PO cattle.
IDENTIFICATION OF POLYMORPHISM OF FSH BETA-SUBUNIT GENE AS SPERM QUALITY MARKER IN BALI CATTLE USING PCR-RFLP Ishak, A.B.L.; Sumantri, C.; Noor, R.R.; Arifiantini, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 4 (2011): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.823 KB) | DOI: 10.14710/jitaa.36.4.221-227

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The aim of study was to identify the association of FSH beta-subunit gene polymorphisms withsperm quality traits. A total of 470 samples of normal mature bull from several breeds were used forpopulation study and 127 bulls from National and Regional AI centre of Indonesia for association study.To amplify, a PCR-RFLP method was used and digested with Pst1 restriction enzyme. The allelefrequency of the A and B in Bali cattle were (0.000) and (1.000), respectively. The absence of otherallele A suggested that the Bali cattle was monomorphic, while Brahman, FH, Simmental and Limousinewere polymorphic. The highest observed heterozygosity were found in Limousine (0.318) and thehighest expected heterozygosity were in Simmental (0.420). The higher incident of percentage of spermabnormalities were found in Simmental, Limousin, Brahman compared to Bali and FH. Among all typesof sperm abnormalities, the abaxial and microcephalus were found in highest number.
Co-Authors A. Anggraeni A. B. L. Ishak, A. B. L. A. Farajallah A. Fuah M. Fuah A. GUNAWAN A. Mardiastuti A. Muktiani A. T. Pratiwanggana, A. T. A.B.L. Ishak A.O. Rini Abuzahra, M. A. Afnan, R. Ahmadi, J. Anneke Anggraeni Ardiyana, M. Ardiyana, M. Arief, I. I. Arif, A. A. Aryani, A. Asnath M. Fuah Bambang Ngaji Utomo Basril, S. Y. Bilyaro, W. Budiman, C. C. Talib D. A. Astuti D. Herdiana D. Rahmat D.T.H. Sihombing Darmati, S. Dewi, M. K. Dudi . E. Andreas E. Damayanthi E. Handiwirawan E.D. Gurnadi F. Saputra, F. Fastawa, R. Furqon, A. Furqon, A. Galib, I. Gurnadi, R. E. H. Hidayati, H. H. Martojo H. Nuraini Habiburahman, R. Habiburahman, R. Hardiyanto, D. Hasibuan, H. Hendraningsih, L. I Supriatna I. Arifiantini I. Inounu Indriastuti, R. J. Jakaria Jakaria Jakaria Jodiansyah, S. K. JAMAL Khaeruddin Khaeruddin Khaerunnisa, I. L. Abdullah L. Praharani Listyarini, K. Lukmanudin, M. M. Imron M. Indriati, M. M. Muladno M. Yamin Mulyono, R. H. Musyaddad, T. N. Hilmia P. D. M. H. Karti Permatasari, P. Pramukti, F. W. R. I. Arifiantini R. Priyanto R. R. Noor, R. R. R. Saepudin R.E. Gurnadi R.R. Noor Rahmadani, R. P. Restu Misrianti Rosyada, Z. N. A. Rukmiasih Rusdin, M. Rustama Saepudin S. Darwati S. Hadisoesilo S. Murtini S. Rahayu, S. S. Subandriyo S. Sutikno Sari, S. A. Setiadi, D. R. Solihin, D. D. Sopian, Y. Suhita, R. L. Sulistyanto, B. Supriyanto, J. Syaikhullah, G. T. Sartika T. Suryati T. Susanti Uddin, M. J. Ulupi, N. Ulupi, N. Widiono, Widiono Yanthi, N. D. Zainatha, F. M. Zamanti, D.