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Activity of Trigonella foenum-graecum on Some Cell Lines. Agustini, Kurnia; Sumaryono, Wahono; Widyanto, R Micho
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Trigonella  foenum-graecum  (TFG)  is  one  of  medicinal  plants  contains  some steroidal sapogenin  such as diosgenin, yamogenin, gitogenin, tigogenin and trigoneoside, also  alkaloid  trigonellin,  which  is  have  many  activity  as  antidiabetic,  estrogenic  and  also as  anti  cancer.    This  experiment  was  done  to  explore  the  activity  of  some  extract  of TFG on some cell lines such as MCF7 (Human Breast Cancer Cell-line), T47D (Human Breast  Cancer  Cell-line),  PC3  (Human  Prostate  Cell-line)  and  SKOV  (Human  Ovarian Carcinoma Cell-line).  This assay was done using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium)  methods.    Results  showed  that  ethyl  acetate  fraction  gives  the lowest  IC50  than  another  extracts.  IC50  for  PC3  is  66.24  ppm,  IC50  for  MCF7  is  41.81 ppm,  IC50  for  T47D  is  58.63  ppm.    These  datas  can  be  used  for  further  research  to isolate the active compound from TFG. Keywords: Trigonella foenum-graecum, MCF-7, T47D, SKOV, PC3.
Isolation and structure elucidation of main compound originated from sponge Axynissa aplysinoides Sumaryono, Wahono; Wibowo, Agung Eru; ., Chaidir
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 4, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.647 KB) | DOI: 10.14499/indonesianjpharm0iss0pp186-191

Abstract

Isolation of the main compound of sponge Axynissa aplysinoides collected from Lombok seawater, has been conducted. Methanol extract of dried sponge was fractionated into nonpolar, semipolar and polar fractions using hexane, ethyl acetate and buthanol. Identification and final purification of the main compound was done using column chromatography and high performance liquid chromatography (HPLC). Structure elucidation of the main compound was performed using mass spectrometry (EIMS), 1H- and 13C-NMR spectroscopies. The main compound was identified as (E)-(4-hydroxystyryl) trimethylammonium.Key words : isolation, structure elucidation, Axynissa aplysinoides
CHARACTERIZATION OF TRYPSIN-LIKE PROTEASE OF LACTOBACILLUS PLANTARUM FNCC 0270 Margono, Trismilah; Sumaryono, Wahono; Malik, Amarila; Sadikin, Mohamad
HAYATI Journal of Biosciences Vol. 21 No. 2 (2014): June 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1468.621 KB) | DOI: 10.4308/hjb.21.2.87

Abstract

Trypsin is an enzyme that has a unique mechanism of cutting peptide bonds specifically at the carboxyl side of lysine or arginine amino acids, with another amino acid. This study aims to analyze a trypsin-like protease (TLP) found in Lactobacillus plantarum FNCC 0270,  by performing partial proteomic tests, i.e. MALDI-TOF/TOF, and standard bioinformatics tools. SDS-PAGE analysis showed 4 protein bands. Two bands of the (P1 and P2) showed molecular weights equivalent to 47.35 and 38.42 kD, each generating 8 and 11 peptide fragments respectively. According to information in www.ncbi.nlm.nih.gov/genbank/structures, the structure  of serine protease HtrA (subs. plantarum L. plantarum ST?III) consists of three domains. Using Clone Manager® software by aligning two sequences we obtained eleven. The Lactobacillus produces of the trypsin-like serine protease has 40-90% similarity. Using the Clustal W2 software we passed the 11 sequences through multiple alignments, and found that the isolate L. plantarum is closely related to L. buchneri, L. brevis, and L. malefermentans on the phylogenetic tree. Alignment analysis results showed that all 8 peptide fragments of band 1 and 11 peptide fragments of band 2,  of the SDS-PAGE, were located in the active domain region of  the fourth trypsin-like serine protease producing Lactobacilli.
Cassava Pulp as a Biofuel Feedstock of an Enzymatic Hydrolysis Proces Djuma’ali, Djuma’ali; Soewarno, Nonot; Sumarno, Sumarno; Primarini, Dyah; Sumaryono, Wahono
Makara Journal of Technology Vol 15, No 2 (2011)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.983 KB) | DOI: 10.7454/mst.v15i2.221

Abstract

Cassava pulp, a low cost solid byproduct of cassava starch industry, has been proposed as a high potential ethanolic fermentation substrate due to its high residual starch level, low ash content and small particle size of the lignocellulosic fibers. As the economic feasibility depends on complete degradation of the polysaccharides to fermentable glucose, the comparative hydrolytic potential of cassava pulp by six commercial enzymes were studied. Raw cassava pulp (12% w/v, particle size <320 μm) hydrolyzed by both commercial pectinolytic (1) and amylolytic (2) enzymes cocktail, yielded 70.06% DE. Hydrothermal treatment of cassava pulp enhanced its susceptibility to enzymatic cleavageas compared to non-hydrothermal treatment raw cassava pulp. Hydrothermal pretreatment has shown that a glucoamylase (3) was the most effective enzyme for hydrolysis process of cassava pulp at temperature 65 °C or 95 °C for 10 min and yielded approximately 86.22% and 90.18% DE, respectively. Enzymatic pretreatment increased cassava pulp vulnerability to cellulase attacks. The optimum conditions for enzymatic pretreatment of 30% (w/v) cassava pulp by a potent cellulolytic/ hemicellulolytic enzyme (4) was achieves at 50 °C for 3, meanwhile for liquefaction and saccharification by a thermo-stable α-amylase (5) was achieved at 95 °C for 1 and a glucoamylase (3) at 50 °C for 24 hours, respectively, yielded a reducing sugar level up to 94,1% DE. The high yield of glucose indicates the potential use of enzymatic-hydrothermally treated cassava pulp as a cheap substrate for ethanol production.
PERANAN RIZOBAKTERI DAN FUNGI MIKORIZA ARBUSKULAR DALAM MENINGKATKAN EFISIENSI PENYERAPAN HARA SORGUM MANIS (SORGHUM BICOLOR L. MOENCH) Rupaedah, Bedah; Anas, Iswandi; Santosa, Dwi Andreas; Sumaryono, Wahono; Budi, Sri Wilarso
Jurnal Ilmu Tanah dan Lingkungan Vol 16 No 2 (2014): Jurnal Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (628.403 KB) | DOI: 10.29244/jitl.16.2.45-52

Abstract

Efisiensi pemakaian pupuk kimia dapat didefinisikan berdasarkan aspek agronomi. Secara umum, semakin banyak hara yang diserap tanaman untuk meningkatkan hasil panen, maka tingkat efisiensi semakin tinggi. Pelacakan jumlah hara yang dapat diserap tanaman dalam meningkatkan produksi adalah komponen kunci untuk mengukur efisiensi hara. Percobaan lapangan dilakukan untuk menilai peranan rizobakteri dan fungi mikoriza arbuskular (FMA) pada berbagai konsentrasi pupuk kimia pada budidaya sorgum manis (Sorghum bicolor L. Moench). Dua galur rizobakteri (Mycobacterium senegalense 73LR dan Bacillus firmus 80JR) serta dua galur FMA (Gigaspora sp. 40MDL dan Glomus sp. 38MDL) digunakan sebagai inokulan dengan penambahan beberapa variasi konsentrasi pupuk kimia. Bobot biomasa, kandungan gula, serapan fosfor, kalium dan nitrogen dianalisis. Inokulasi rizobakteri mampu meningkatkan tinggi tanaman dan kandungan P pada taraf p<0.01, sedangkan bobot biomassa, kandungan gula, serapan P dan K pada taraf p<0.05. Inokulasi FMA saja berpengaruh nyata terhadap bobot biomassa dan kandungan N pada taraf p<0.01, sedangkan kandungan gula pada taraf p<0.05. Sementara itu, interaksi rizobakteri dan FMA mampu meningkatkan bobot biomassa, kandungan dan serapan K, serta efisiensi penyerapan hara N. Secara keseluruhan pengaruh inokulasi rizobakteri, FMA dan pupuk kimia serta interaksi faktor-faktor tersebut dapat meningkatkan bobot biomasa, kandungan gula, serapan P dan K, serta efisiensi penyerapan hara N oleh tanaman sorgum manis. Dengan demikian, pemakaian rizobakteri dan FMA berpotensi meningkatkan efisiensi penggunaan pupuk kimia, sehingga diharapkan dapat mengurangi biaya produksi budidaya sorgum manis.
Peranan rizobakteri dan fungi mikoriza arbuskular dalam proses fotosintesis dan produksi gula sorgum manis (Sorghum bicolor L. Moench) Role of rhizobacteria and arbuscular mycorrhizal fungi in increasing photosynthesis process and sugar production of sweet sorghum (Sorghum bicolor L. Moench) RUPAEDAH, Bedah; ANAS, Iswandi; SANTOSA, Dwi Andreas; SUMARYONO, Wahono; BUDI, Sri Wilarso
E-Journal Menara Perkebunan Vol 83, No 1: Juni 2015
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.72 KB) | DOI: 10.22302/iribb.jur.mp.v83i1.13

Abstract

AbstractDual inoculation effects of rhizobacteria andAMF as well as the addition of chemical fertilizerson photosynthesis were studied by analyzingprocess of CO2 gas exchange, chlorophyll content,nutrient uptake, sugar content and growth of sweetsorghum. AMF inoculation as a single cultureincreased carbon assimilation, stomatal conductance,intercellular CO2 concentration, phosphoruscontent, chlorophyll content, sugar content, plantheight, shoot weight and mycorrhizal colonization.Interaction of AMF and rhizobacteria increasedsugar content of sweet sorghum stems andpotassium content of sweet sorghum leaves,whereas its interaction with chemical fertilizerssignificantly increased chlorophyll content, sugarcontent and mycorrhizal colonization of sorghumplant roots. Interaction of AMF, rhizobacteria andchemical fertilizers increased sugar content, rootweight and mycorrhizal colonization. The use ofAMF solely or its interaction with rhizobacteriaand chemical fertilizers had a great potential inimproving photosynthesis process of sweetsorghum. The process is associated with increasingcrop productivity, such as sugar content ofsorghum which is potential as a source ofrenewable energy.AbstrakPengaruh inokulasi ganda rizobakteri danFMA dengan penambahan pupuk kimia dipelajaridengan cara menganalisis proses pertukaran gas CO2, kandungan klorofil, kandungan hara dan gula,pertumbuhan dan produktivitas sorgum manis.Inokulasi FMA sebagai kultur tunggal dapatmeningkatkan asimilasi karbon, konduktansistomata, konsentrasi CO2 interselular, kandunganfosfor, kandungan klorofil, kandungan gula, tinggitanaman, berat batang dan derajat kolonisasimikoriza. Interaksi antara rizobakteri dan FMAdapat meningkatkan kandungan gula batang dankalium daun sorgum manis, sedangkan interaksinyadengan pupuk kimia dapat meningkatkan kandunganklorofil dan gula serta derajat kolonisasimikoriza pada perakaran sorgum manis. Sementaraitu, interaksi FMA, rizobakteri dan pupuk kimiadapat meningkatkan kandungan gula batang, beratakar dan derajat kolonisasi mikoriza pada perakaransorgum manis. Penggunaan FMA baik sendirimaupun interaksinya dengan rizobakteri dan pupukkimia memiliki potensi besar dalam meningkatkanproses fotosintesis sorgum manis. Proses tersebutberkaitan dengan peningkatan produktivitas sorgummanis dalam hal ini kandungan gula sorgum manisyang berpotensi sebagai sumber energi terbarukan.
TRANSFORMASI PLASMID PTRLI DENGAN TEKNIK ELEKTROPORASI PADA ASPERGILLUS TERREUS DAN UJI STABILITAS TRANSFORMAN hardianto, dudi; gusnidar, tutus; singgih, marlia; musadad, amir; sumaryono, wahono
Jurnal Sains dan Teknologi Indonesia Vol 14, No 1 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.551 KB) | DOI: 10.29122/jsti.v14i1.901

Abstract

Aspergillus terreus is a Saprophyte fungus that produces several secondary metabolites as lovastatin (anti-cholesterol drug) and itaconic acid (a polymer material). Lovastatin is one of the statin class of drugs that have efficacy as antihypercholesterolemic. Plasmid transformation is the introduction and incorporation of exogenous plasmid into cells orprotoplast. In this study, pTRLI plasmid (pTRI inserts containing lovE gene as a regulator gene in the biosynthesis of lovastatin) will be transformed by electroporation transformation. The purpose of this research is transformation of pTRLI plasmid into protoplasts of Aspergillus terreus by electroporation and obtain stable transformants. The research was initiated by isolation of pTRLI plasmid. Then pTRLI plasmid was determined purity and concentration by nanodrop. Furthermore, Protoplasts of Aspergillus terreus were isolated enzymatically by adding an enzyme which can degrade the cell wall of Aspergillus terreus which contains chitin and cellulose. PTRLI plasmid were transformed into protoplasts of Aspergillus terreus by electroporation. These transformants were grown in Czapek-Dox medium containing pyrithiamine agar and the number of transformants mg-1 of pTRLI plasmid was calculated. Transformants were selected to grow in Czapek-Dox medium containing piritiamin 1 mg l-1. The number of transformants produced 187 transformants mg-1 of PTRLI plasmid. Transformants are stable up to five generations by growing the transformants in Czapek-Dox medium agar containing piritiamin 1 mg l-1. The success of the transformation indicated by ptrA gene in transformants that can be amplified by PCR. The size of fragment DNA is 801 bp.
Analisis Urea-Kreatinin Tikus Putih pasca Pemberian Ekstrak Buah Mahkota Dewa dan Herba Pegagan SUMARYONO, WAHONO; WIBOWO, AGUNG ERU; NINGSIH, SRI; AGUSTINI, KURNIA; SUMARNY, ROS; AMRI, FITRIANIAR; WINARNO, HENDIG
JURNAL ILMU KEFARMASIAN INDONESIA Vol 6 No 1 (2008): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

The evaluation of toxicity of a mixed herbal extract containing mahkota dewa fruits (Phaleria macrocarpa Scheff. Boerl) and pegagan leaves (Centella asiatica L. Urban) on Wistar-strain rats had been carried out by the determination of the urea and creatinine content in urine and plasma after feeding. Oral doses of 100 mg, 500 mg, and 2500 mg of the mixed extract/kg body Weight were administered for 16 consecutive weeks to three groups of rats. Each treated group consisted of 15 males and 15 females, and the control group was represented by 10 males and 10 females. Samples of urine and plasma of the treated groups were taken at the time right before treatment (Week zero) and at Sm, 16th, 18th Week, while those of the control were taken at zero Week, 8th, and 16th Week, respectively. The result showed that theurea and creatinine contents among the treated and control groups were not signihcantly different. It could be concluded that oral administration ofthe mixed extract by a dose up to 2500 mg/kg body weight for 16 Weeks did not influence the urea and creatinine contents both in urine and plasma of the treated animals. Based on this result, it could be assumed that the use of the mixed extract is safe.
Transformasi Plasmid pTRLI ke dalam Protoplas Aspergillus terreus dengan Penambahan Polietilenglikol HARDIANTO, DUDI; SINGGIH, MARLIA; MUSADAD, AMIR; SUMARYONO, WAHONO; GUSDINAR, TUTUS
JURNAL ILMU KEFARMASIAN INDONESIA Vol 10 No 1 (2012): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

Transformasi plasmid merupakan proses masuknya plasmid ke dalam protoplas atau sel inang yang mengakibatkan perubahan materi genetika. Tujuan dari penelitian untuk mentransformasi plasmid pTRLI ke dalam protoplas Aspergillus terreus dan menentukan stabilitas transforman. Penelitian diawali dengan isolasi plasmid pTRLI, penentuan kemurnian dan konsentrasi plasmid pTRLI dengan menggunakan serapan pada panjang gelombang 260 dan 280 nm dari alat nanodrop. Kemudian, protoplas A. terreus diisolasi secara enzimatik dengan enzim kitinase, selulase, dan maserosim. pTRLI ditransformasi ke dalam protoplas A. terreus dengan penambahan kalsium klorida dan polietilenglikol. Transforman ditumbuhkan dalam media Czapek Dox agar yang mengandung piritiamin 1 mg/L. Jumlah transforman yang dapat tumbuh antara 12 sampai 19 transforman/µg plasmid pTRLI. Transforman dideteksi dengan mengamplilikasi gen ptrA yang berukuran 801 pb. Dari penelitian ini, dapat disimpulkan bahwa PEG dapat digunakan untuk mentransformasi plasmid pTRLI ke dalam protoplas A. terreus dan transforman stabil sampai generasi ke S.
Evaluasi Penerapan CDOB sebagai Sistem Penjaminan Mutu pada Sejumlah PBF di Surabaya AGUSTINI, VANNINA; UTAMI, WAHYU; SUMARYONO, WAHONO; ATHIYAH, UMI; RAHEM, ABDUL
JURNAL ILMU KEFARMASIAN INDONESIA Vol 15 No 1 (2017): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

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Abstract

Cara Distribusi Obat yang Baik (CDOB) adalah sebuah pedoman yang bertujuan untuk memastikan mutu sepanjang jalur distribusi sesuai persyaratan dan tujuan penggunaannya. Pedagang Besar Farmasi (PBF) dalam menjalankan pendistribusian obat wajib menerapkan 9 aspek CDOB. Tujuan dari penelitian ini adalah untuk mengetahui sejauh mana PBF telah menerapkan 9 aspek tersebut. Penelitian ini merupakan penelitian deskriptif. Sampel penelitian adalah 41 PBF di Surabaya yang dipilih menggunakan teknik random sampling dan menggunakan instrumen penelitian berupa kuisioner. Data diambil pada bulan Agustus hingga September tahun 2015, dimana pada saat itu PBF belum diharuskan memiliki sertifi kat CDOB. Berdasarkan hasil penelitian dapat dilihat bahwa 40 (97,56%) PBF belum memiliki sertifi kat CDOB. Dari 41 PBF yang menjadi responden, 18 (43,90%) PBF telah memenuhi ≥80% CDOB , 16 (39,02%) PBF telah memenuhi ≥65 - <80% CDOB , 6 (14,63%) PBF memenuhi ≥50 - <65% CDOB dan 1 (2,44%) PBF memenuhi <50% CDOB. Satu (2,44%) PBF tidak pernah melakukan kajian manajemen mutu, 4 (9,76%) Apoteker Penanggung Jawab (APJ) tidak pernah mengikuti pelatihan CDOB, 5 (12,20%) PBF yang semua personelnya belum pernah mendapatkan pelatihan CDOB, 5 (12,20%) PBF tidak pernah melakukan pemeriksaan NIE (Nomor Ijin Edar), 2 (4,88%) PBF jarang menyimpan obat sesuai suhu penyimpanan yang tertera pada kemasan. Dari hasil penelitian ini dapat disimpulkan bahwa belum seluruh PBF di Surabaya menerapkan 9 aspek CDOB, sehingga dapat secara langsung maupun tidak langsung berpengaruh terhadap mutu obat yang didistribusikan oleh PBF tersebut. Keharusan PBF memiliki sertifi kat CDOB baru ditetapkan pemerintah pada akhir November 2017.