Articles

Taksiran Kerugian Ekonomi Penyakit Kluron Menular (Brucellosis) pada Populasi Ternak di Indonesia (THE ESTIMATION OF ECONOMIC LOSSES CAUSED BY BRUCELLOSIS IN LIVESTOCK POPULATION IN INDONESIA) Basri, Chaerul; Sumiarto, Bambang
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.269 KB) | DOI: 10.19087/jveteriner.2017.18.4.547

Abstract

Brucellosis is one of the constraints in development of animal husbandry in Indonesia and is thought to have a huge economic impact. This study aimed to calculate the national economic losses cause by brucellosis in livestock in Indonesia. The method of calculating the estimated economic losses used two ways, by calculating the direct losses and by calculating the price of economic externalities. Source of secondary data for the calculation derived from scientific publications, government data, expert opinions, market data, and assumptions that are based on expert discussions. The results showed that by the direct calculation method it is estimated the economic losses at approximately Rp. 3,516,401,986,082 per year whilst by the second method the losses is estimated at Rp. 3,637,773,925,000 per year. Both methods of calculation used give relative similar economic losses estimation. The total loss due to brucellosis is estimated to reach 1.8% of the total value of livestock assets in Indonesia.
Deteksi Produksi Toksin Stx-1 dan Stx-2 dari Escherichia coli O157:H7 Isolat Lokal Hasil Isolasi Feses dan Daging Sapi Suardana, I Wayan; Krisna Erawan, I Gusti Made; Sumiarto, Bambang; Widaya Lukman, Denny
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Shiga toxin produced by Escherichia coli O157:H7 can cause outbreaks and sporadic cases of serioushuman diseases. The diseases are indicated by hemorrhagic colitis and hemolytic uremic syndrome. Meatand meat products have been identified as vehicles of food borne disease caused by E.coli O157:H7. Themain aim of this research was to identify the correlation between the level of E.coli O157:H7 contaminationand the presence of Shiga toxin (Stx1 and Stx2) by applying method of Vero toxin Escherichia coli-ReversePassive Agglutination Test (VTEC-RPLA). The results showed that 3 of 7 isolates and 1 of 4 isolatesisolated from feces of cattle and beef, respectively produced Stx 1 (VT1). In the detection of Stx 2 (VT2), 4of 7 isolates and 1 of 4 isolates, isolated from the same samples were found to produce this toxin.According to all isolates, in this research showed, 1 isolate was found to produce VT2, 4 isolates to produceboth VT1 and VT2, while 6 isolates showed negative results either to VT1 or VT2.
Pembelian Ternak dan Kelembaban Tinggi Merupakan Faktor Risiko Leptospirosis pada Sapi di Girimulyo, Kulon Progo, Jogjakarta (PURCHASE OF CATTLE AND HIGH HUMIDITY WERE RISK FACTORS OF BOVINE LEPTOSPIROSIS IN GIRIMULYO, KULON PROGO DISTRICT) Mulyani, Guntari Titik; Sumiarto, Bambang; ., Yuriati
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Leptospirosis is an acute infectious disease could to attack humans and animals such as dogs, cats,cows, pigs, and wild animals such as rats, weasels and squirrels. The death rate of human leptospirosis inIndonesia reached 2.5 to 16.5%. Leptospirosis attacked approximately 274 residents, 18 fatalities atKulonprogo disctrict, and designated as Extraordinary Events in 2011. The purpose of this reasearch is todetermine risk factors affecting the prevalence of leptospirosis in cattle in the area of human leptospirosisin Girimulyo, Kulon Progo. This study used 132 samples of cattle blood. Methods of examination withMicro Aglutination Test (MAT) conducted at the Center for Veterinary Research, Bogor. Micro AglutinationTest is done by creating a serum dilution with PBS in stages then added antigen in the form of various cultures leptospira serovar. Subsequently incubated at 28-30 ° C for 2 hours, and was reading the resultsunder a microscope dark field / phase contrast. Endpoint reading was 50% agglutination, highest finalserum dilution in serum-antigen mixtures that showed 50% agglutination is called the titer. Serum witha titer of 1:100 or more against one or more tested positive for serovar. Micro Aglutination Test result wasthe dependent variable (Y), while the independent variables (X) were factors of farmers, animal factors,enclosure factors, and feed factors. Univariate analysis was done to see descriptively each variable studied.Association between the prevalence of leptospirosis and risk factors was analyzed by Chi Square.Multivariate Logistic Regression Analysis was used to build models of leptospirosis disease to investigatethe risk factors associated with leptospirosis. Variables that influence the prevalence of leptospirosiswere measured magnitude of association by Odd Ratio. The results of study that the prevalence of bovineleptospirosis in Girimulyo subdistrict, Kulonprogo district was 7.6%. Risk factors for the prevalence ofleptospirosis in cattle in Girimulyo subdistrict, Kulonprogo district were livestock purchased factor (OR5.49) and a high humidity factor (OR 1.65). These results can be concluded that leptospirosis infection incattle is high (7.6%) in the area of human leptospirosis. The possibility cattle as a source of infection inhumans still needs further research.
Isolasi and Identification of Bacteria from the Urine Fluid Organic Fertilizer (POC)Ettawa Crossbred (PE) in The Sleman Regency Suwito, Widodo; Wahyuni, A.E.T.H.; Nugroho, Widagdo Sri; Sumiarto, Bambang; Bekti, Utomo Bimo
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.569 KB) | DOI: 10.22146/jsv.3781

Abstract

Urine is a by product from Ettawa crossbred (PE) goat farm in Sleman regency. In addition, the manure urine goats can be used for fluid organic fertilizer (POC). Recently, organic farming is more interesting for people because it does not use any chemical fertilizers. Salmonella sp and E. coli O157:H7 are pathogenic bacteria that can contaminate agricultural products and dangerous for public health. One of sources of contamination in agricultural products is due to the organic fertilizer. Therefore, the aim of the present study was to determine pathogenic bacteria from POC urine PE goats in Sleman regency. A total of 8 POC samples were collected from PE goat urine in Sleman regency. These samples were analyzed for Salmonella sp, E. coli O157:H7 and other pathogenic bacteria by using biochemical reactions. All samples are negative for Salmonella sp. and E. coli O157:H7 as well. Whereas, 85% of 8 samples are positive for Pseudomonas sp. The present study showed that POC from PE goats farm in Sleman regency are negative for pathogenic bacteria.  
Isolasi dan Sensitifitas Antibiotika terhadap Streptococcus spp dari Kambing PE Mastitis Subklinis Kronis Suwito, Widodo; Wahyuni, AETH; Nugroho, Widagdo Sri; Sumiarto, Bambang
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.714 KB) | DOI: 10.29244/avi.6.1.8-15

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Penelitian ini dilakukan untuk isolasi dan sensitifitas antibiotika terhadap Streptococcus spp dari susu kambing PE mastitis subklinis kronis di Kabupaten Sleman, Yogyakarta. Sebanyak 10 sampel susu kambing PE mastitis subklinis kronis dari Kabupaten Sleman digunakan dalam penelitian ini. Kriteria kambing PE mastitis subklinis kronis dengan uji California Mastitis Test (CMT) dua kali yaitu saat laktasi dan laktasi berikutnya dengan hasil positif (++) atau (+++) yang diikuti dengan penghitungan jumlah sel somatik (JSS) dengan metode Breed. Isolasi Streptococcus spp., dengan kultur dalam media blood agar selanjutnya di inkubasikan pada suhu 37°C selama 24 jam. Sensitifitas antibiotika terhadap Streptococcus spp., dengan agar difusi menggunakan kertas cakram antibiotika yang sudah diketahui konsentrasinya. Saat uji CMT pertama terdapat dua sampel positif (++) dan delapan sampel positif (+++) dengan rataan JSS masing-masing 2.400.000 sel/ml dan 4.475.000 sel/ml, sedangkan uji CMT kedua terdapat empat sampel positif (++) dan enam sampel positif (+++) dengan rataan JSS masing-masing 2.775.000 sel/ml dan 4.550.000 sel/ml. Susu kambing PE mastitis subklinis kronis dari Kabupaten Sleman dapat diisolasi Streptococcus spp., sebanyak 8 isolat dengan tipe hemolitik α, β, dan γ masing-masing 2, 1, dan 5 isolat. Streptococcus spp., resisten terhadap penisilin sedangkan terhadap tetrasiklin, ampisilin, eritromisin, dan sulfonamide masih sensitif. Penelitian ini menunjukkan bahwa Kambing PE mastitis subklinis kronis dari Kabupaten Sleman disebabkan oleh Streptococcus spp., dengan penisilin resisten, sedangkan tetrasiklin, ampisilin, eritromisin, dan sulfonamide masih sensitif.
Diagnosis and Molecular Marker Analysis of Bali’s Rabies Virus Isolates (DIAGNOSIS DAN ANALISIS PENANDA MOLEKULER VIRUS RABIES ISOLAT BALI) Dibia, I Nyoman; Sumiarto, Bambang; Susetya, Heru; Putra, Anak Agung Gde; Mahardika, I Gusti Ngurah Kade; Scott-Orr, Helen
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The direct fluorescent antibody test (dFAT) was recommended by both World Health Organization(WHO) and Office International des Epizooties (OIE) as a standard diagnostic technique for rabies. Sincethe outbreak of rabies in Bali, it was ascertain the importance to develop a reverse transcriptase-polymerasechain reaction (RT-PCR) technique with specific primers as an alternative diagnostic method. The aim ofthis study was to develop a RT-PCR technique for rabies diagnosis in animals and find out the molecularmarker of Bali’s rabies virus (BRV) isolates based on the sequence of nucleoprotein (N) gene. Brainsamples were obtained during 2009 from 14 suspected rabid dogs and one cattle, where rabies viruseswere isolated. The dFAT was used to detect the presence of rabies viral antigen. Ribonucleic acid (RNA) ofrabies viruses was extracted with TRIzol reagent. Fragment of N gene was amplified using one-step RTPCRmethod with specifically-designed primer pairs and sequenced using ABI automatic sequencer. Multiplealignment of nucleotide and deduced amino acid sequences were analyzed using ClustalW of MEGA 4.0program. This study found that twelve out of fifteen animal brain samples confirmed as rabies by dFAT.Similarly, a single band of 1215 bp PCR product for rabies virus was also detected in twelve out of twelve(100%) dFAT rabies positive samples. It is therefore evident that alternative diagnostic of rabies inanimals can be established using RT-PCR technique. The results showed that the RT-PCR has a very highagreement with dFAT. Polymorphic sites of N gene of twelve BRV isolates were identified at the position186, 501, 801, 840, 1068 and 1153. Bali’s rabies virus isolates have conserved amino acid (isoleucine)alterations at position 308 (open reading frame). Isoleucine distinguished between all Bali’s isolates andthe all of isolates from other area of Indonesia and other part of the world. This finding significantlydifferent as compared to other rabies virus isolates from other part of Indonesia or the world documentedon the GenBank. Accordingly it is proposed that it can be used as molecular marker and believed to be thefirst study of molecular marker of rabies virus in Indonesia.
Penilaian Risiko Kualitatif Masuk dan Tersebarnya Rabies pada Anjing ke Kota Sorong Provinsi Papua Barat Kurniawan, Muchammad Taufik; Susetya, Heru; Sumiarto, Bambang
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13116.929 KB) | DOI: 10.22146/jsv.39538

Abstract

The rabies outbreaks of Indonesia in these two decades tends to spread faster to other islands/regions. Trade trafficking and people's habits of carrying dogs between islands are contributing factor that triggers rabies cases in Provinces previously free of rabies. This study aims to qualitative risk assessment entry and spread of rabies in dogs into Sorong City of West Papua Province. Methods of collecting primary data obtained from expert opinions, questionnaires, interviews, and direct observation in the field. Secondary data is taken through search of scientific publications, surveillance results, unpublished data in the form of reports, and documents from authorized agencies. The results of study showed that release assessment was high, the incidence of rabies in dogs was 52%, and 180 cases of rabies in humans (lyssa) in Sulawesi, Maluku and North Maluku. The exposure assessment was high based on the presence of rabies transmite animal (RTA) traffic from endemic areas, 58% dogs, 38% cats, and 4% apes from Java 68% (Surabaya 50%, Jakarta 18%), Sulawesi 10% (Manado and Bitung), Maluku 14% (Ambon) and North Maluku 8% (Ternate). The consequence assessment is high because there is a single impact that is categorized as nationally significant. The estimated risk of getting into rabies is high. The potential pathways used in RTA traffic to Sorong City is by sea at 89.3% and air at 10.7%. The results of the study concluded that the qualitative risk assessment of entry and spread of rabies in dogs to the Sorong City of West Papua Province was high. All risk assessments have low uncertainty.
The Prevalence and Risk Factors of White Spot Syndrome Virus in Tiger Shrimp at Traditional Ponds (PREVALENSI DAN FAKTOR RISIKO WHITE SPOT SYNDROME VIRUS PADA TAMBAK UDANG WINDU TRADISIONAL) Taslihan, Arief; Sumiarto, Bambang; Nitimulyo, Kamiso H.
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A research has been conducted to determine the status of the White Spot Syndrome Virus (WSSV)epidemic on traditional shrimp ponds in Demak regency. A cross-sectional research method was selected,by calculating samples using statistical calculation based on the assumption of 70% prevalence rate anderror (p<10%). Additionally, this research also determined the risk factors in the onset of WSSV shrimppond. Surveyed areas included Sayung and Bonang, subdistrict in Demak district, because the area areactively engaged with shrimp farming. A total of 90 traditional tiger shrimp ponds were sampled in thisstudy. The results showed that the prevalence of WSSV infected farms was 65.6% (Confident Interval,CI=55.7%-75.4%). Factors that affected high prevalence of WSSV were screening of seed (Coefficient=1.517,P<0.049), pond size (coefficient=0.0002, P<0.0089) and drying ponds (coefficient=3.756, P<0.001). Factorsthat affected the production of shrimp in ponds were analysed using multivariate regression analysis.Based on the analysis, some factors had an important role in production, i.e. : stadia of shrimp seed(coefficient=19.091, P<0.19), water depth in pond (coefficient=1.343, P<0.0024) and WSSV-infected shrimp(coefficient=-44.369, P<0.001). It was concluded that WSSV prevalence in studied area was 65.6%, and itconsidered as hyperendemic. Factors affected the WSSV prevalence were selection of seed, pond’s size anddrying ponds. Risk factors affected shrimp production in the pond were stocking seed stadia, whereasWSSV-infection of shrimp was negatively affected factor.
PENGOPTIMALAN METODE KROMATOGRAFI CAIR KINERJA TINGGI DALAM ANALISIS SENYAWA DELTAMETHRIN SEBAGAI RESIDU DALAM PRODUK ASAL HEWAN Satria, R. Gagak Donny; Sumiarto, Bambang; Trisyono, Andi; Wijayanti, Dwi
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.933 KB) | DOI: 10.21157/j.ked.hewan.v8i1.1264

Abstract

Penelitian ini bertujuan mendapatkan prosedur atau metode kromatografi cair kinerja tinggi (KCKT) yang valid dan optimal dalam analisis deltamethrin sebagai senyawa yang berpotensi menjadi residu dalam produk hewan. Alat utama yang digunakan dalam penelitian ini adalah satu set KCKT Shimadzu 6.1, dengan kolom C-18 (30° C), panjang gelombang detektor UV-vis 236 nm. Fase gerak yang digunakan dalam penelitian ini adalah asetonitril 80% dalam akuabides yang dialirkan dengan laju 1,25 ml/menit. Hasil penelitian menghasilkan kromatogram yang terlihat menunjukkan peak area yang nyata terpisah dari senyawa lain. Batas deteksi diketahui pada konsentrasi 0,1 μg/ml, sedangkan batas kuantifikasi pada konsentrasi 0,5 μg/ml. Rerata luas area untuk konsentrasi 0,5; 1; 1,5; 2; 5; dan 10 μg/ml masing-masing adalah 18.255,33; 47.142,00; 55.587,00; 64.181,33; 204.269,00; dan 395.918,00 dengan persamaan garis linier y= 39.866x-1.719,5 (R= 0,99). Hasil analisis juga menunjukkan presisi dan akurasi hasil yang baik. Hasil penelitian dapat disimpulkan bahwa metode yang dikembangkan pada penelitian ini mempunyai validitas yang baik dan optimal untuk analisis deltamethrin, yang merupakan senyawa potensial menjadi residu pada produk asal hewan.
Tingkat dan Faktor Risiko Kekebalan Protektif terhadap Rabies pada Anjing di Kota Makassar (RATE AND RISK FACTORS OF PROTECTIVE IMMUNITY AGAINST RABIES ON DOGS IN THE CITY OF MAKASSAR) Utami, Sri; Sumiarto, Bambang
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The rate and risk factors on protective immunity against rabies were conducted in Makassar city. Theaims of this study were to determine the level of immunity of dog and find out the association betweenmanagement factors and the level of maintenance of the dog immunity against rabies. Two hundred andforty-six serum samples were collected for measurement of dog immunity level with a method of enzymelinked immunosorbent assay (ELISA). Maintenance management data were gathered throughquestionnaires and direct observation. Analysis of data obtained by the level of immunity was donedescriptively. The significance association between the risk factors and the strength of immunity level wascalculated by using Chi square (X2) and odds ratios. The results showed only 12.2% of dogs had a protectiveantibody against rabies at > 0.5 IU/ml. Factors affecting the level of protective immunity against rabieswere the first vaccination at 1-6 months age (OR = 18.6), post-vaccination after 1-6 months (OR = 6.0), themaintenance location of dogs in urban areas (OR = 4.5), dog caring management in house (OR = 3.8), andowner income above Rp. 2.000.000/month (OR = 3.0). It is concluded that the low level of protective immunityagainst rabies and the dog bites reported each year indicates that Makassar, as endemic areal of rabies,is a threat to the outbreak of the disease in animals and humans to the surrounding areas.
Co-Authors A.E.T.H. Wahyuni Adiana Mutamsari Witaningrum, Adiana Mutamsari Aeth Wahyuni AETH Wahyuni Wahyuni, AETH Wahyuni Agnesia Endang Tri Hastuti Wahyuni Anak Agung Gde Putra Andi Trisyono Arief Taslihan Asmarani Kusumawati Callinan, Richard Chaerul Basri Charles Rangga Tabbu Denny Widaya Lukman Doddi Yudhabuntara Dwi Wijayanti Dyah Ayu Widiasih Effendi, Mustofa Helmi Eko Sutarti Ekowati Handharyani Erwan Agus Purwanto Estu Widodo, Estu F.A. Sudjadi Guntari Titik Mulyani Helen Scott-Orr Henricus Roby Cahya Putra, Henricus Roby Cahya Heru Susetya Heru Susetyo I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Nyoman Dibia I Wayan Suardana Ismadi, Joko Juwari J, Juwari Kamiso H Nitimulyo Kamiso H. Nitimulyo KAMISO HANDOYO NITIMULYO Kurniasihl . Kurniawan, Muchammad Taufik NI WAYAN KURNIANI KARJA Nugroho, Widagdo Sri Nugroho Sri Permatasari, Dian Ayu Purwaningsih Purwaningsih Purwaningsih, . Putri, M. Th. Khrisdiana R. Daru Maya, F. Lintang R. Gagak Donny Satria Retno Damajanti Soejoedono Roza Azizah Primatika, Roza Azizah Samkhan Samkhan, Samkhan Setyawan Budiharta Setyo Budhi Siti Khairul Saadah Hanif Sivalingham, Prisha Lini Sophia Setyawati Sri Hartati Sri Utami Subronto Prodjoharjono Sugiwinarsih S, Sugiwinarsih Surya Agus Prihatno Susan M. Noor Torribio, Jenny-Ann Tri Anggraeni Kusumastuti Tri Untari Utomo Bimo Bekti Wardiarto Darjoprawito, Wardiarto Wayan Tunas Artama Wibisono, Freshinta Jellia Widagdo Sri Nugroho Widagdo Sri Nugroho Widodo Suwito Widya Asmara Yatri Drastini Yuriati .