Articles

PENGARUH PENCUCIAN SEL TERHADAP PERTUMBUHAN DAN NILAI NUTRISI CHAETOCEROS GRACILIS Chilmawati, Diana; Suminto, Suminto
Buletin Oseanografi Marina Vol 1, No 2 (2012): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.669 KB) | DOI: 10.14710/buloma.v1i2.11222

Abstract

Kuantitas dan kualitas sel diatom, Chaetoceros gracilis menjadi pembatas utama dalam produksi perikanan di hatchery.  Diduga kultur diatom mengalami pertumbuhan yang tidak stabil karena terkontaminasi oleh bakteri atau mikroorganisme lain.  Sel diatom tersebut akan mengalami penurunan produksi dan nilai nutrisinya dan apabila digunakan sebagai pakan alami akan menghambat pertumbuhan dan perkembangan larva.  Salah satu cara untuk memecahkan masalah tersebut adalah dengan pencucian sel bibit / inokulan diatom yang dibudidayakan. Tujuan dari penelitian ini adalah mengkaji pengaruh pencucian sel terhadap pola pertumbuhan sel diatom (C. gracilis) dan nilai nutrisi atau kandungan protein sel diatom tersebut pada fase kepadatan maksimum. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan yaitu tanpa pencucian sel; satu kali pencucian sel; dua kali pencucian sel dan tiga kali pencucian sel. Hasil penelitian menunjukkan bahwa pencucian sel berpengaruh nyata (p<0,05) terhadap pola pertumbuhan sel diatom (C. gracilis) dimana dapat mempersingkat durasi waktu adaptasi, memperpanjang fase stasioner dan menghasilkan kepadatan sel maksimum lebih tinggi.  Pencucian sel juga dapat meningkatkan nilai nutrisi atau kandungan protein sel diatom (C. gracilis) pada fase kepadatan maksimum.   KATA-KATA KUNCI : pencucian sel diatom, Chaetoceros gracilis, pertumbuhan dan nilai nutrisi
DIFFERENT PERCENTAGES OF GONAD MATURITY ON THE FERTILITY AND HACHING RATE IN ARTIFICIAL BREEDING OF ABALONE (HALIOTIS ASININA) Suminto, Suminto; Sani, Dyah Anggun Permana; Susilowati, Titik
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 5, No 2 (2010): Jurnal Saintek Perikanan
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.882 KB) | DOI: 10.14710/ijfst.5.2.54-60

Abstract

Abalone is one of the marine mollusk that has high economic value. Mass abalone cultivation requires the mature gonads broodstock to produce seeds, but the maturity of the gonads of male and female often can not be simultaneously. Artificial insemination using a solution of ammonia is one solution to produce abalone seed in the hatchery. Ammonia solution can increase motility, fertilization rate of eggs and abalone seed production, so the provision of seeds for cultivation of abalone is independent from nature. The research of artificial insemination was carried out at various levels of gonadal maturation dams abalone (H. asinina) to determine the percentage of fertility and hatching rate of eggs from each Gonad Maturity Level. In stadia gonadal recovery, no fertilization occurs, whereas in the gonads maturing stadia had fertilization and hatching rate 24.33 ± 2.08% and 22.12 ± 2.18%, respectively at ripe stadia, gonads had been fertilization and hatching rate were 94.67% ± 1.53% and 82.82% ± 3.58%, wasn?t recorded in the stadia spent fertilization did not occur because the eggs were released in culture media. Gonad Maturity Level, thus can be used in artificial insemination was the ripe gonadal stadia, which had the highest percentage of both levels of fertilization and hatching rate with a diameter ranging from 189.6-252.8 ?m. Key Words: Abalone (H. asinine); Artificial breeding; Gonad Maturity Level; Hatching Rate
PSYCHOLOGICAL PRINCIPLE AND ITS IMPLICATIONS IN ISLAMIC EDUCATION HASAN LANGGULUNG'S PERSPECTIVE Suminto, Suminto
AL-HAYAT: Journal of Islamic Education Vol 3 No 2 (2019): AL-HAYAT: Journal of Islamic Education
Publisher : AL-HAYAT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35723/ajie.v3i2.79

Abstract

The psychological principle of education can be understood as truth which is the basis or basis for thinking, both at the planning, implementation and evaluation stages of knowledge based on theories from psychology. Therefore, it becomes essential in understanding education, that education also has principles as a place and foothold that is upright, upright in material, upright in interaction, upright in innovation, or honest in its ideals. The type of research used is library research. The content analysis is used as an analysis method in this study. The results of this study can be concluded that: (1) The concept of psychological principles in Islamic education according to Hasan Langgulung is by emphasizing the theory of the learning process and the idea of human creation according to the Islamic view, which includes the nature of creation, essential human potential, cognitive and psychological growth and development and human spirit, so that it can be understood that human beings are creatures consisting of elements of Islamic and spiritual. (2) The implication of the concept of psychological principles in Islamic education is to view learning as a process in preparing the young generation so that they can act as the next generation, transferring knowledge and Islamic values ??so that they are aligned with the purpose of the human being created, as well as in their daily behaviour. Therefore, in the process of education must pay attention to the development of the soul, as well as the growth of the body of students by referring to the basis, objectives, curriculum, material, and evaluation that carry human functions as abid and khalifatullah which are decorated with righteous deeds.
PENGARUH TEPUNG TELUR AYAM AFKIR PADA PAKAN BUATAN YANG BERPROBIOTIK TERHADAP EFISIENSI PEMANFAATAN PAKAN, PERTUMBUHAN DAN KELULUSHIDUPAN LELE DUMBO (CLARIAS GARIEPINUS). (THE EFFECT OF CHICKEN EGGS REJECTS POWDER IN ARTIFICIAL FEED WITH ADDED PROBIOTIC ON EFFICIENCY FEED UTILIZATION, GROWTH AND SURVIVAL RATE OF DUMBO CATFISH (CLARIAS GARIEPINUS)) Suminto, Suminto; Susilowati, Titik; Wibowo, Bambang Argo; Chilmawati, Diana
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 13, No 2 (2018): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.778 KB) | DOI: 10.14710/ijfst.13.2.111-118

Abstract

Pengembangan budidaya ikan lele, Clarias gariepinus sering terjadi kendala dengan biaya pakan yang terlalu tinggi (60-70% dari biaya produksi). Barangkali, penggunaan bahan baku lokal seperti pemanfaatan tepung telur ayam yang nilai nutrisinya tinggi, mudah didapat, dan murah harganya merupakan salah satu solusinya. Tujuan penelitian ini adalah untuk mengkaji dan mengetahui pengaruh penambahan tepung telur ayam afkir dalam pakan buatan yang berprobiotik terhadap efisiensi pemanfaatan pakan, pertumbuhan dan kelulushidupan ikan lele dumbo (C. gariepinus). Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap (RAL) yang menggunakan  4 perlakuan dan masing-masing 3 kali kali ulangan. Perlakuan itu adalah pakan yang tanpa menggunakan tepung telur ayam afkir (Perlakuan A), pakan yang menggunakan tepung telur afkir masing-masing sebanyak 15%, 30%, dan 45% sebagai Perlakuan B, C, dan D. Ikan uji  bibit lele dumbo (C. gariepinus) yang ditebar mempunyai bobot rerata 2.04±0.05 g dengan kepadatan 1 ekor/L yang dipelihara selama 42 hari. Variabel data yang diukur meliputi Tingkat Konsumsi Pakan (TKP), Efisiensi Pemanfaatan Pakan (EPP), Feed Convertion Ratio (FCR), Protein Efficiency Ratio (PER), Relative Growth Rate (RGR), dan Survival Rate (SR). Hasil penelitian ditunjukkan bahwa penggunaan tepung telur ayam afkir pada pakan buatan memberikan pengaruh yang nyata (p < 0.05) terhadap TKP, EPP, FCR, PER, dan RGR, tetapi tidak berpengaruh nyata (P?0,05) terhadap SR. Perlakuan dengan penambahan tepung telur ayam afkir sebanyak 30% (Perlakuan C) memberikan nilai terbaik untuik TKP sebesar 146,87%, EPP sebesar 88,77%, PER sebesar 2,61% dan RGR sebesar 7,65%/hari dari perlakuan lainnya. Monitoring nilai kualitas air pada media pemeliharaan telah menunjukkan bahwa pada kisaran yang layak untuk pemeliharaan ikan uji. Catfish cultivation development, Clarias gariepinus often occur constraints with the high cost of feed  (60-70% of the production cost). Perhaps, the use of local raw materials such as the utilization of rejected chicken egg with high nutritional value, easy to obtain, and cheap price is one of the solution. The purpose of this research was to know the effect of addition of rejected chicken egg  powder inprobiotized artificial feed on the efficiency of feed utilization, growth and survival rate of catfish, C. gariepinus. This study was used an experimental method with completely randomized design (RAL) by using 4 treatments and each of them 3 replications. The treatments were feed without use of rejected chicken egg powder  (Treatment A), feed using 15%, 30%, and 45% of powder  meals of rejected chicken egg  as treatment B, C, and D, respectively. Catfish, C. gariepinus seeds were stocked with a mean weight of 2.04 ± 0.05 g with a density of 1 tail / L and cultured  for 42 days. The data variables measured were  Total of Feed Consumption (TFC), Feed Utilization Efficiency (FUE), Feed Conversion Ratio (FCR), Protein Efficiency Ratio (PER), Relative Growth Rate (RGR), and Survival Rate (SR). The results showed that the use of chicken egg starch in artificial feed gave a real effect (p <0.05) on TFC, FUE, FCR, PER, and RGR, but no significant effect (P?0,05) on SR. Treatment with the addition of 30% chicken meal (Treatment C) had the best value for TFC at 146.87%, the FUE of 88.77%, the PER of 2.61%  and the RGR of 7.65% / day than of the another treatments. Monitoring of  water quality values on maintenance media has shown that at a reasonable range for the maintenance of catfish culture.
EFFECT OF BAKERS YEAST, VITAMIN B12, AND VITAMIN C AS NUTRITIONAL IMPROVEMENT OF FOOD ON THE DENSITY PRODUCTION OF BRACHIONUS PLICATILIS Chilmawati, Diana; Suminto, Suminto
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 5, No 2 (2010): Jurnal Saintek Perikanan
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.9 KB) | DOI: 10.14710/ijfst.5.2.47-53

Abstract

The important factor of seedling production is live food with good quality. One way to improve quqlity of live food, Brachionus plicatilis, to gain a high quality of larvae in seedling production, is through implementing food enrichment. Chlorella sp, which is its live food, can be enriched by combination of baker?s yeast, vitamin B12 and vitamin C, which then given, in order to increase Branchionus plicatilis growth and nutrition content. Purpose of this study is to acknowledge comparative population growth and improvement of Brachionus plicatilis with different kinds of foods enrichment, to understand the right food enrichment composition for Brachionus plicatilis growth. This research are laboratory experimental, with 10 ind/ml early spread density on 5 litres capacity container. The experiment using a Completely Randomize Design with 4 treatment and 3 repetitions. That four treatments included A (Chlorella sp), B (Chlorella sp + baker?s yeast), C (Chlorella sp + baker?s yeast + vitamin B12), D (Chlorella sp + baker?s yeast + vitamin B12 + vitamin C). Result from the research shows that implementation of food enrichment, which using baker?s yeast, vitamin B12 and vitamin C, brings about a great significant differences (p<0,01) in each treatment to population growth. D is the best treatment with specific growth rate of 0,661, maximal density of 530,4 ind/ml and final density of 381,7 ind/ml. Key Words: Elements of Food Enrichment, Population, Brachionus plicatilis
USING THE LIVE FOOD ENRICHMENT WITH THE SQUID EGG EXTRACT ON THE QUANTITY AND QUALITY OF ROTIFER, BRACHIONUS PLICATILIS O.F. MULLER Suminto, Suminto
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 3, No 2 (2008): Jurnal Saintek Perikanan
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.167 KB) | DOI: 10.14710/ijfst.3.2.64-73

Abstract

The Objective of the research was to know the effect of live food enrichment of rotifer with squid egg extract on the growth patterns and quality of rotifer, especially the EPA and DHA contents. The experiment method was employed in this research and it carried out in the aquaculture laboratory, Faculty of Fisheries and Marine Sciences, Diponegoro University. The research planning was a completely randomized design with five treatments and three replicates, respectively. The recording data was analyzed to get the variables of rotifer growth patterns and the most of rotifer samples were analyzed EPA and DHA contents in the chemical and biochemical laboratory center, Gajah Mada University. The results showed that the addition of squid egg extract in the culture media of rotifer was highly significant effect (p<0.01) on the specific growth rate, the maximal density, and the final density of rotifer culture. However, there was also highly significsnt effect (p<0.01) on the nutrition quality through EPA and DHA contents. To increase the quantity and quality of rotifer, B. plicatilis O.F. Muller in the culture was addition of squid egg extract of 1,2 g/1 million ind. Rotifers during twelve two hours in the rotifer culture. Key word : Rotifer, Brachionus plicatilis O.F. Muller, enrichment, quantity and quality, EPA dan DHA
PERTUMBUHAN BAKTERI PROBIOTIK ALKALIGENUS SP. DAN FLAVOBACTERIUM SP. YANG DIISOLASI DARI USUS UDANG PADA MEDIA KULTUR MOLASE DAN KAOLIN Suminto, Suminto
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 4, No 1 (2008): Jurnal Saintek Perikanan
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.578 KB) | DOI: 10.14710/ijfst.4.1.21-27

Abstract

Penelitian ini bertujuan untuk mengetahui konsentrasi tetes tebu (molase) dan tepung kaolin yang tepat untuk media kultur massal bakteri Alkaligenes sp. dan Flavobacterium sp. pada kondisi lingkungan (suhu, salinitas, dan pH) yang terbaik bagi pertumbuhan. Metoda yang digunakan pada penelitian ini adalah eksperimen dengan   3 (tiga) macam perlakuan dan masing-masing dilakukan pengulangan sebanyak 3 (tiga) kali. Perlakuan perbedaan  konsentrasi  molase yaitu M1 (16 gr/mL), M2 (32 gr/mL), dan  M3 (48gr/mL). Sedangkan perlakuan perbedaan konsentrasi tepung kaolin adalah K1 (8 gr/mL), K2 (16 gr/mL), dan K2 (32 gr/mL). Data yang dikumpulkan dalam penelitian ini adalah kelimpahan bakteri yang diukur melalui Opical Density pada spectrofotometer.  Analisa statistik uji varian, uji wilayah  ganda duncan telah digunakan dalam analisa data penelitian ini untuk mengetahui pengaruh perbedaan berbagai kondisi lingkungan media kultur. Hasil penelitian menunjukkan bahwa perlakuan perbedaan konsentrasi molase berpengaruh sangat nyata (p<0,01) terhadap pertumbuhan bakteri Alkaligenes sp. dan Flavobacterium sp. Pada perlakuan perbedaan konsentrasi tepung kaolin menunjukkan adanya pengaruh yang sangat nyata (P<0,01) terhadap pertumbuhan bakteri Alkaligenes sp., tetapi tidak berpengaruh nyata terhadap pertumbuhan bakteri Flavobacterium sp.   Kata Kunci : Bakteri probiotik, pertumbuhan, jenis media, kondisi lingkungan
PERFORMA PERTUMBUHAN DAN PRODUKSI TELUR OITHONA SIMILIS DENGAN PAKAN FITOPLANKTON BERBEDA PADA SALINITAS MEDIA KULTUR OPTIMUM (GROWTH PERFORMANCE AND EGG PRODUCTION OF OITHONA SIMILIS WITH DIFFERENT DIET OF PHYTOPLANKTON CELLS IN CULTURE MEDIUM OPTIMUM) Chilmawati, Diana; Hutabarat, Johannes; Anggoro, Sutrisno; Suminto, Suminto
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 16, No 1 (2020): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.16.1.39-44

Abstract

Kondisi optimum media kultur dan diet pakan yang sesuai dengan kebutuhan hidup (ekofisiologis) Oithona similis sangat penting agar dapat tumbuh dan berkembang secara maksimum. Proses bioenergetika dalam upaya pemanfaatan energi pakan untuk pertumbuhan, erat hubungannya dengan proses osmoregulasi organisme air yang dipengaruhi oleh salinitas media kulturnya. Tujuan penelitian ini adalah mengkaji pengaruh perbedaan diet fitoplankton dan menentukan jenis fitoplankton yang tepat yang memberikan performa  pertumbuhan dan produksi telur O. similis terbaik pada kondisi salinitas media kultur yang optimum (19,4 permil). Penelitian ini menggunakan metode eksperimental dengan  Rancangan Acak Lengkap yang terdiri dari 4 perlakuan dengan masing-masing 5 kali ulangan, yaitu: kultur O. Similis dengan diet fitoplankton A. Chlorella vulgaris; B. Nannochloropsis oculata; C. Isochrysis galbana; dan D. Chaetoceros calcitrans. Hasil penelitian menunjukkan bahwa perbedaan diet mikroalga berpengaruh terhadap pertumbuhan dan produksi telur O. similis pada kondisi salinitas media kultur optimum. Pemberian sel diatom Chaetoceros calcitrans memberikan kepadatan total (16,88±0,32 ind./ml), laju pertumbuhan populasi (0,141±0,001/hari) dan produksi telur (24,50±0,58 telur/ind.) O. similis terbaik.
PENGARUH PROBIOTIK KOMERSIAL PADA PAKAN BUATAN TERHADAP PERTUMBUHAN, EFISIENSI PEMANFAATAN PAKAN, DAN KELULUSHIDUPAN BENIH IKAN GURAMI (OSPHRONEMUS GOURAMY) D35-D75 THE EFFECTS OF COMMERCIAL PROBIOTIC IN ARTIFICIAL FEED ON THE GROWTH, FEED UTILIZATION Suminto, Suminto; Chilmawati, Diana
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 11, No 1 (2015): JURNAL SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.578 KB) | DOI: 10.14710/ijfst.11.1.11-16

Abstract

ABSTRAK   Ikan gurami (Osphronemus gouramy) merupakan komoditas ikan air tawar yang bernilai ekonomis penting, tetapi mempunyai kendala didalam budidaya, misalnya pertumbuhannya lambat  dan pemanfaatan pakan yang kurang efisien.  Salah satu pemecahan masalahnya  adalah dengan pemanfaatan probiotik pada pakan  didalam kultur, khususnya pada stadia larva gurame (Osphronemus gouramy). Penelitian ini bertujuan untuk mengetahui dan mengkaji pengaruh penambahan probiotik komersil terhadap pertumbuhan, efisiensi pemanfaatan pakan dan kelulushidupan larva ikan gurame (Osphronemus gouramy). Materi yang digunakan adalah beberapa  produk probiotik komersial yang dicampurkan pada pakan buatan ikan dengan cara disemprotkan dan setelah didiamkan selama 24 jam diberikan sebagai pakan gurame. Metoda eksperimen dan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 4 kali pengulangan telah dikembangkan pada penelitian ini. Perlakuan yang diujikan adalah tanpa pemberian probiotik pada pakan (A); pemberian probiotik 1 (B); pemberian probiotik 2 (C); dan perlakuan D dengan pemberian probiotik 3 pada pakan. Berat benih gurame (Osphronemus gouramy) per individu 1,86±0,97 g dan panjang 4,11±0,41 cm. Padat penebaran benih gurame pada perlakuan yaitu 2 ekor/liter. Benih gurame diberi makan 3 kali sehari sebanyak 5% dari bobot biomassa. Hasil penelitian menunjukkan bahwa penambahan probiotik pada pakan memberikan pengaruh nyata (P<0,05) terhadap pertumbuhan dan efisiensi pemanfaatan pakan, tetapi tidak berpengaruh nyata (P>0,05) terhadap kelulushidupan benih ikan gurame. Hasil terbaik diperoleh pada pemberian probiotik 1 dengan nilai tingkat pertumbuhan spesifik (SGR) sebesar 1,67±0,42 %,  efisiensi pemanfaatan pakan (22,2±2,6%) dan kelulushidupan (85,0±5,6%). Kriteria kualitas air media kultur masih baik untuk mendukung proses pertumbuhan dan pemanfaatan pakan.   Kata kunci : Probiotik komersial, pakan buatan, gurame (Osphronemus gouramy), dan pertumbuhan   ABSTRACT   Gouramy (Osphronemus gouramy) is  freshwater fish commodity that have high economic value, but it have problems in the culture such as the slow growth and inefficiency of feed utilization.  One of the problem solves is utilization of probiotic bacteria in the artificial feed, especially for fish larvae culture. The purpose of this study was to know and observe the effects of adding commercial probiotics for growth, feed utilization eficiency and survival rate of gouramy (Osphronemus gouramy) from day 35  to day 75. Some commercial probiotics used were sprayed onto artificial feed and after incubated during 24 hours, then give as fish feed in the culture. The experimental methods with completely randomized design (CRD) were employed in this research. The design consisted of 4 treatments and 3 replication, respectively. The treatments tested were without  probiotic added (treatment A), with probiotic 1 (B), with probiotic 2 (C), and the treatment D with added by probiotic 3. The initial density of  fish larvae were 2 inds/liter of water medium with average weight of 1,86 ± 0,27 g and length of 4,15±0,35 cm.  Gouramy fish were fed three times every day with 5% from biomass weight. The  research results shown that the difference adding probiotics in the artificial feed were significantly effects (P<0.05) on the growth and feed utilization efficiency but no significantly effect (P>0,05) on the survival rate of  gouramy (Osphronemus gouramy) in the culture. The best result was in the treatment B (with added by probiotic 1), where  the  best SGR of 1,67±0,42 %,  feed utilization efficiency of 22,2 ± 2,6% and survival rate of  85,0 ± 5,6%.  The water quality criteria of  culture medium were the good supported to the growth, feed  utilization, and survival rate of Gouramy (Osphronemus gouramy) in the culture.  Keywords : Commercial probiotic, artificial feed, Gouramy (Osphronemus gouramy), and growth 
THE ADDITION OF AMMONIA SOLUTION WITH DIFFERENT CONCENTRATIONS IN THE SPERMS ON THE PERCENTAGE OVULATION EGG OF ABALONE, HALIOTIS ASININA Suminto, Suminto; Nuriman, Agus Archan; Chilmawati, Diana
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 5, No 1 (2009): Jurnal Saintek Perikanan
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.172 KB) | DOI: 10.14710/ijfst.5.1.31-37

Abstract

The aim of this research was to know the impact of addition of ammoniak in different concentration in the sperm on the percentage of egg ovulation. The research method was carried out by completely randomized design with four treatments and three replicates, respectively. Those treatments were the addition of ammoniac solution with concentration of 0% (treatment A), 0.005% (treatment B), 0.010% (treatment C), and 0.015% (treatment D). The data was analyzed by using ANOVA and Duncan?s Multiple Range Test to know the effect of between the other treatments. The results showed that was the highly significant difference effect (p<0.01) on the egg ovulation of Abalone (H. asinina). The highest egg ovulation rate was obtained in treatment B (94.67% ). The ovulation rate was significantly higher than those in treatments C, D, and A, respectively. According to the results, the best concentration of the ammonia solution to the abalone sperm added was 0.005%. Furthermore, the regression test also showed a positive, linear relationship between spermatozoa motility rate and egg ovulation rate. However, the analysis of orthogonal polymonial provided a ammonia solution with the highest ovulation rate of 0.0057%.  Keywords: Concentration, ammonia solution, ovulation egg, abalone, H. asinina