Articles

Found 28 Documents
Search

PENGGUNAAN XILANASE STREPTOMYCES SP. 45 I-3 AMOBIL UNTUK HIDROLISIS XILAN TONGKOL JAGUNG [IMMOBILIZATION OF EXTRACELLULAR XYLANASE FROM STREPTOMYCES SP. 45 I-3 FOR HYDROLYSIS OF CORNCOB XYLAN ] Sunarti, Titi Candra; Mutia, Ferry; Gusmawati, Niken Financia; Lestari, Yulin; Meryandini, Anja
Jurnal Teknologi dan Industri Pangan Vol. 20 No. 1 (2009): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.721 KB)

Abstract

Xylan extraction from corncob is done by using alkaline as solvent. Xylan extraction from corncob could give the yields as 10.9%. One percent of corncob xylan is used as substrate to produce the xylanase, compared to oatspelt xylan. Immobilization of xylanase was performed using 1% EudragitTM S100 solution (w/v), with 5:1 volume ratio of xylanase and 1 % EudragitTM S100 (w/v). Activity of the immobilized xylanase was decreased to 23.97% compared with free xylanase. Immobilized xylanase have optimum pH and temperature at 6.0 and 40°C  respectively, have also thermal stability at 30?40°C for an hour. Immobilized xylanase could be reused, but its activity decreased to 52.38% after 3 times application.
PENAMBAHAN BAKTERI ASAM LAKTAT TERENKAPSULASI UNTUK MENEKAN PERTUMBUHAN BAKTERI PATOGEN PADA PROSES PRODUKSI TAPIOKA Rembulan, Glisina Dwinoor; Sunarti, Titi Candra; Meryandini, Anja
Jurnal Teknologi dan Industri Pangan Vol. 26 No. 1 (2015): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.973 KB) | DOI: 10.6066/jtip.2015.26.1.34

Abstract

Lactic acid bacteria (LAB) produce organic acids and active compounds which can inhibit the growth of pathogenic bacteria. Lactic acid bacteria potentially can be introduced to inhibit pathogenic bacteria in the tapioca production at the extraction stage, especially during the settling process since there is possibility of starch slurry to be contaminated by pathogenic bacteria from water. The objectives of this research were to design a solid starter of LAB through encapsulation by using modified starch includes sour cassava starch, lintnerized cassava starch and nanocrystalline starch, utilize the starter for suppressing the growth of pathogenic bacteria in the production process of tapioca and characterize the functional properties of tapioca. The encapsulation of lactic acid bacteria was conducted by freeze drying at a temperature of -50°C for 48 hours. The viability of LAB after freeze drying with sour cassava starch matrix was 92% of the liquid starter, with lintnerized cassava starch matrix was 93%, while that with nanocrystalline matrix was 96%. After application of the LAB culture during settling process for tapioca extraction and the tapioca was stored at room temperature for 6 months, it was shown that E. coli, Salmonella and Shigella were  detected in the native tapioca starch (without treatment) while the starch added with lactic acid bacteria starter was not absent for the pathogenic bacteria. The addition of lactic acid bacteria in extraction process can suppress the growth of pathogenic bacteria in tapioca. The results showed that lintnerized cassava starch matrix is the best matrix because after 6 months it still contained lactic acid bacteria as compared to liquid starter and that encapsulated with other matrixes.
Evaluation of Growth and Physiological Responses of Three Rice (Oryza sativa L.) Varieties to Elevated Temperatures Kurniawan, Dede Yudo; Junaedi, Ahmad; Lubis, Iskandar; Sunarti, Titi Candra
Journal of Tropical Crop Science Vol 6 No 01 (2019): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.944 KB)

Abstract

Temperature is a primary factor that affects the rate of plant development and has great impacts on plant growth, metabolism, and yield. A study was conducted to analyze the effects of elevated temperature on rice morphological and the physiological growth. The research was arranged in a nested randomized block design consisting of two factors, temperatures and rice varieties. Elevated temperatures were provided through the uses of different materials of plastic roof and walls to have an average and maximum temperature of 27.6 °C and 41.6 °C (T1); 28.1°C and 43.8 °C (T2), and 29.5°C and 47.1 C (T3), respectively. The study used three varieties of rice, “Ciasem”, “Ciherang”, and “IR64”. All rice varieties showed signifi cant increases in tiller number per hill and shoot dry weight, but had a decrease in the stomatal conductance, transpiration rate, and SPAD values at grain fi lling stage with the increasing temperatures. The number of tiller per hill increased when temperature was elevated from 27.6 to 28.1 and 29.5°C by about 29.9 and 21.3%, respectively.
Rekayasa Proses Hidrolisis Pati dan Serat Ubi Kayu (Manihot utilissima) untuk Produksi Bioetanol Susmiati, Yuana; Setyaningsih, Dwi; Sunarti, Titi Candra
Agritech Vol 31, No 4 (2011)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.652 KB) | DOI: 10.22146/agritech.9648

Abstract

Ethanol production from cassava (Manihot utilissima) usually uses enzymatic process for starch hydrolysis. Enzymatichydrolysis by α-amylase and amyloglucosidase enzymes are not able to convert cassava fibers into sugars. Dilute acid hydrolysis is applied to convert both starch and fibers, which will increase the yield of simple sugars as fermentable sugars and resulting in high ethanol production. In this research there are two steps of dilute acid hydrolysis, first for starch hydrolysis at H SO concentration of 0.1-0.5 M, 5-15 minutes and second for fiber hydrolysis at 0.5-1.0 24M H SO , 10-20 minutes, at the same temperature of 121-127 oC and pressure of 1.0-1.5 atm. The disadvantage of acid hydrolysis is the formation of toxic compounds such as hydroxymethyl furfural (HMF) which is inhibited yeast fermentation. Therefore, acid hydrolyzates were detoxified with NH OH before use as fermentation substrate. The best starch hydrolysis condition was obtained at 0.4 M H SO  for 10 minutes which gave 257.37 g/l of total sugars, 24229.38 g/l of reducing sugars, 89.59 of dextrose equivalent (DE) and 0.57 g/l of HMF. While the best fiber hydrolysis performed at 1.0 M H SO  solution for 20 minutes which gave 79.74 g/l of total sugars, 70.88 g/l of reducing sugars, 2488.99 of DE and 0.0142 g/l of HMF. Single direct acid hydrolysis was the most suitable substrate for yeast fermentationwith the ethanol concentration of 5.7 % (w/v) and 30.5 % (w/w) of ethanol yield. This result is comparable with enzymatic hydrolysis which gave ethanol yield of 30 % (w/w).ABSTRAKProduksi etanol dari ubi kayu biasanya menggunakan enzim untuk menghidrolisis pati. Hidrolisis secara enzimatismenggunakan enzim α-amilase dan amiloglukosidase tidak mampu mengkonversi serat menjadi gula. Hidrolisis asam  berkonsentrasi  rendah  dilakukan  untuk  mengkonversi  pati  dan  serat,  sehingga  gula-gula  sederhana  yang dapat difermentasi meningkat dan menghasilkan produksi etanol tinggi. Pada penelitian ini ada dua tahap hidrolisis menggunakan asam berkonsentrasi rendah, yaitu tahap pertama untuk menghidrolisis pati dengan konsentrasi H SO 0,1-0,5 M selama 5-15 menit dan tahap kedua untuk menghidrolisis serat dengan kensentrasi H SO  0,5-1,0 M selama 2410-20 menit pada suhu dan tekanan sama, yaitu 121-127 oC dan 1,0-1,5 atm. Kekurangan pada hidrolisis asam adalahterbentuknya senyawa toksik seperti hidroksimetil furfural (HMF) yang mengganggu fermentasi khamir. Oleh karena itu hidrolisat asam didetoksifikasi menggunakan NH OH sebelum digunakan sebagai substrat fermentasi. Kondisi terbaik hidrolisis pati diperoleh pada konsentrasi H SO  0,4 M selama 10 menit dengan nilai total gula 257,37 g/l, 24gula pereduksi 229,38 g/l, dextrose equivalent (DE) 89,59 dan HMF 0,57 g/l. Selain itu kondisi terbaik hidrolisis serat diperoleh pada konsentrasi H SO  1,0 M selama 20 menit dengan nilai total gula 79,74 g/l, gula pereduksi 70,88 g/l, 24DE 88,99 dan HMF 0,0142 g/l. Hidrolisat asam yang paling sesuai digunakan sebagai substrat fermentasi adalah darihidrolisis satu tahap tanpa pemisahan serat yang menghasilkan etanol dengan konsentrasi 5,7 % (b/v) dan rendemen etanol 30,5 (b/b). Hasil tersebut tidak berbeda jauh dengan hidrolisis enzimatis yang mengasilkan rendemen etanol 30% (b/b).
PRODUCTION AND CHARACTERISTICS OF YEAST DEXTRANASE FROM SOIL Hijah, Vestika Iskawati Wahidul; Sunarti, Titi Candra; Meryandini, Anja
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (543.647 KB) | DOI: 10.4308/hjb.26.1.26

Abstract

  The existence of dextran in sugar cane juice is a major problem in the sugar industry, causing substantial losses. Treatment of dextran through enzymatic hydrolysis using dextranase is highly recommended as the most suitable method at this time because this is more effective and more economical. This study investigated the production and characterization of dextranase from local isolate yeast to degrade dextran on sugar cane juice. The selected yeast was identified on the basis of molecular identification. Dextranase was produced from the culture with the best carbon and nitrogen sources then was characterized. Application of enzyme was also evaluated. As a selected isolate, F4 had the closest relationship with Pichia kudriavzevii. The highest production of dextranase was induced by the supplementation of glucose and combination of yeast extract and peptone. The enzyme had optimum working condition at pH 7, temperature at 30°C and it is more stable at 4°C of storage temperature. The cation Na+ played key role as co-factor while K+ and Ca2+ were detected as inhibitor of the enzyme. Dextranase from F4 isolate can hydrolyze dextran both in pure and in mixed dextran substrate, but with a lower hydrolysis rate.
SIFAT FUNGSIONAL PATI GARUT HASIL MODIFIKASI HIDROKSIPROPILASI DAN TAUT SILANG [FUNCTIONAL PROPERTIES OF HYDROXYPROPYLATED AND CROSSLINKED ARROWROOT STARCH] Maulani, Rijanti Rahaju; Fardiaz, Dedi; Kusnandar, Feri; Sunarti, Titi Candra
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (662.141 KB) | DOI: 10.6066/jtip.2013.24.1.60

Abstract

 Dual-modified arrowroot starch using hydroxypropylation and cross-linking methods was carried out to overcome the deficiency in of native arrowroot starches for food processing application. The modification applied the combination concentration of propylene oxide (8, 10, and 12%) and ratio of sodium trimetaphosphate (STMP):sodium tripolyphosphate (STPP) (1%:4%, 2%:5%, and 3%:6%). The resulting dual-modified arrowroot starches had lower gelatinization temperature (68.45?70.00ºC) than that of native arrowroot starch (72.85ºC). The modified arrowroot starches also had a higher peak viscosity (>5500 cp) than that of native arrowroot starch (4209 cP). Breakdown and setback viscosity of modified arrowroot starch was higher values than the native. At acidic pH, the viscosity decreased at different levels of temperature changes as compared to that of normal pH. Modified starch made with 8% propylene oxide and ratio of STMP: STPP 2%:5% and 3%:6%; as well as that made with 10% propylene oxide and 1% STMP:4% STPP had the lowest syneresis tendency. Decrease in the paste clarity occured with increasing concentration of STMP:STPP. The sedimentation volume of the modified starch was higher (29.17-35.83%) than  that of native starch (28.08%), except for those made with 1% STMP: 4% STPP at concentration of propylene oxide 8% and 12%. The gel strength increased (61.77-78.97 gf) at 8% propylene oxide, but decreased (66.50-47.77 gf) at higher concentrations.  
KARAKTERISTIK ISOLAT BAKTERI PENGHASIL DEKSTRAN DARI BATANG TEBU (SACCHARUM OFFICINARUM L.) Akram, Sitti Rahbiah; Sunarti, Titi Candra; Meryandini, Anja
Jurnal Ilmu Pertanian Indonesia Vol. 24 No. 2 (2019): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.871 KB) | DOI: 10.18343/jipi.24.2.160

Abstract

Dextran is a long chain of carbohydrate polymers synthesized by dextransucrase enzyme with sucrose as a substrate. The physical, chemical conditions, temperature, and the concentration of sucrose in the isolate bacterial growth media play an important role in dextransucrase production. This study aims to isolate and characterize the dextransucrase producing bacteria. The bacteria were isolated from sugar cane. TBT 3.2 Isolate which had the highest viscosity (1.48 cP) and crude dextran (7.87 g/L) production was selected. TBT 3.2 Isolate was identified as Paenibacillus polymyxa with 99% similar identity. The Paenibacillus polymyxa TBT 3.2 was characterized based on their ability to produce dextran and cell number. The bacteria had the optimum growth conditions on the media for producing dextran at pH 7, temperature 30°C, and concentration of 20% sucrose. The highest dextransucrase activity and the highest specific activity were obtained after 25 minutes of incubation, with the activities of 29.41 mU/mL and 0.46 U/mg. Based on the results of this study, the Paenibacillus polymyxa TBT 3.2 can be used as a dextran producing bacteria.
KEMASAN ANTIMIKROB UNTUK MEMPERPANJANG UMUR SIMPAN BAKSO IKAN Warsiki, Endang; Sunarti, Titi Candra; Nurmala, Lala
Jurnal Ilmu Pertanian Indonesia Vol. 18 No. 2 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.454 KB)

Abstract

Active packaging is a technique that can be developed to prolong shelf-life or enhance safety or sensory properties, while maintaining the quality of fresh products inside. This experiment produced active packaging of antimicrobial (AM) film in which an antimicrobial (AM) agent was added into the film (instead of food) and then used it to coat the food product. The AM agents slowly release into the product surrounds, inhibit the growth of microbes thus packing and preserving were done in a single step. Carragenan was chosen as a matrix film with garlic extract as an AM agent. Fish balls were chosen as a product to be coated by this AM film. The study consisted of 2 steps i.e.: (i) prepared AM film, and (ii) coated the fish balls with AM film and then tested the quality during storage. In general, the quality of fish balls before and after storage still meet the SNI 0-3819-1995. In the application, the fish balls with AM film has longer shelf life than that without AM. Further more, the result of organoleptic test on appearance of odor and color showed that the panelists prefered coated fish balls than the uncoated one. The panelist prefered coated fish ball which is stored at 5 and 15°C. 
PERUBAHAN STRUKTUR PATI GARUT (MARANTA ARUNDINACEAE) SEBAGAI AKIBAT MODIFIKASI HIDROLISIS ASAM, PEMOTONGAN TITIK PERCABANGAN DAN SIKLUS PEMANASAN-PENDINGINAN [STRUCTURE CHANGES OF ARROWROOT (MARANTA ARUNDINACEAE) STARCH AS INFLUENCED B Faridah, Didah Nur; Fardiaz, Dedi; Andarwulan, Nuri; Sunarti, Titi Candra
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 2 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (691.346 KB)

Abstract

The effects of lintnerization (2.2 N HCl, 2 hours), debranching with pullulanase (1.3 U/g and 10.4 U/g starch) and/or three-auctoclaving-cooling cycles at 121oC for 15 minutes on the changes of arrowroot starch structures were studied. The structural modifications of amylose and amylopectin were measured by Gel Permiation Chromatography (GPC), and the distribution of degree of polimerization (DP) was analyzed by Fluorophore-Assisted Capillary Electrophoresis (FACE). The GPC profile of native starch using Toyopearl HW-65S gel gave mainly two fractions. Fraction I (Fr. I) was a high molecular weight component and Fraction II (Fr. II) was a low molecular weight component. After acid modification, the carbohydrate content of Fr. II increased while that of Fr. I decreased. The amount of  DP of 6 to 8 increased in all modified arrowroot starches. The GPC and FACE analyses showed that all starch modification techniques caused the structural changes of amylopectin molecules to form short chain amyloses.
Thermal Properties, Crystallinity, and Oxygen Permeability of Na-montmorillonite Reinforced Plasticized Poly(lactic acid) Film Yuniarto, Kurniawan; Purwanto, Yohanes Aris; Purwanto, Setyo; Welt, Bruce A.; Purwadaria, Hadi Karia; Sunarti, Titi Candra
Makara Journal of Technology Vol 20, No 1 (2016)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.08 KB) | DOI: 10.7454/mst.v20i1.3105

Abstract

Introducing unmodified organically clay/Na-montmorillonite (Na-MMT) was applied into plasticized poly(lactic acid) PLA  to  produce  film  composites  by direct  casting.  Film  composite  structure,  the  crystallinity  degree  and  form, and thermal  properties  were  carried  out  using  X-ray  diffraction  and differential  scanning  calorimetry.  The effect  of NaMMT  to  the  tortuous path  and  the  crystallinity  degree  in  the  plasticized  film  composites  were calculated  in oxygen barrier  properties.  Chromatogram  film  composites resulted  in  an  intercalated  structure  that  showed  peak diffraction angle shift at about 0.2o. Then, a peak diffraction pattern was indicated in  Î±-form crystal structure. Plasticized PLA has a crystallinity degree at 34%, and the addition of  Na-MMT increased to 52%. Glass transition temperature improved from 53 °C to 57 °C, and melting temperature remained stable at 167 °C. Filling Na-MMT into plasticized PLA caused to enhance a tortuous path about 28% and improved the oxygen permeability to 80%. As a result, the addition of NaMMT of 3% into plasticized PLA during  film composite preparation using the mixing method resulted in balancing properties related to the crystallinity degree, thermal properties, and oxygen barrier properties.