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PREPARASI DAN KARAKTERISASI KATALIS MO-NI/HZ DENGAN METODE IMPREGNASI UNTUK CRACKING KATALITIK MINYAK LIMBAH CAIR PENGOLAHAN KELAPA SAWIT MENJADI BAHAN BAKAR NABATI Lestari, Shinta; Sundaryono, Agus; Elvia, Rina
Alotrop Vol 3, No 1 (2019)
Publisher : FKIP University of Bengkulu

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This study aims to determine the characteristics of Mo-Ni/HZ catalysts and test the ability of Mo-Ni/HZ catalysts in converting vegetable oils into biofuels through catalytic cracking reactions.Catalyst preparation is carried out by the impregnation method which begins with activation of natural zeolite.Impregnation is carried out by dissolution, reflux, filtration, drying and calcination.Catalyst characterization included the determination of the distribution of metals in zeolites using IR spectroscopic analysis and total acidity of the catalyst by ammonia adsorption gravimetric method.Preparation of samples prior to cracking is heating, degumming and bleaching.Catalytic cracking was carried out at 280oC using Mo-Ni/HZ catalyst for 100 minutes.The constituent components of the cracking results were analyzed by GC-MS and IR spectroscopy.The results obtained from the catalytic cracking process were then determined by physical characteristics which included density, viscosity, fog point and pour point test.Based on the shift of wave number in IR spectroscopy, it can be concluded that the Ni and Mo metals are embedded in the HZ.This Mo-Ni/HZ catalyst has total acidity of 8.39 mmol/gammonia.The results of the study prove that the Mo-Ni/HZ experiment proved to be able to crack oil frompalm oil processing wastewater by 91.46% to composition C=O ester CH aliphatic.The physical characteristics of cracking productshave parameters density of 0.93 g/cm3, a viscosity of 26.60 cSt, a fog point of 15oC and pour point of 11.67oC.
UJI FRAKSI ETIL ASETAT DAUN PERONEMA CANESCENS TERHADAP PLASMODIUM BERGHEI PADA MUS MUSCULUS Ramadenti, Fuji; Sundaryono, Agus; Handayani, Dewi
Alotrop Vol 1, No 2 (2017)
Publisher : FKIP University of Bengkulu

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[DETERMINATION OF ETHYL ACETATE FRACTION FROM Peronema canescens LEAF EXTRACT  AGAINST Plasmodium berghei  INFECTED  Mus musculus] This study was conducted to identify secondary metabolite compounds contained in ethanol extract and ethyl acetate fraction from Peronema canescens leaf (sungkai) and to measure the effect of ethyl acetate fraction on anti plasmodium  activity on Mus musculus infected by  Plasmodium berghei. The ethyl acetate extract was obtained by maceration of leaves with 96% ethanol solution in3 days followed by fractionation by ethyl acetate for further test. 25 of male white  M. musculus  with the weight 20-40 g and 6-8 weeks old , divided into 5 groups with 5 head / group. Groups 3, 4 and 5 are treated and groups 1 and 2 are controls. The negative control will be given food and drink only, and for positive control group will be given chloroquine with dose  0,028,  0,056, and  0,084 g / KgBW.  Development of levels of parasitemia were calculated by by observing a thin blood smear with giemsa staining under a microscope , observed until the parasitemia in the blood had reached 20-30% to be given a 3-day follow-up treatment, and then for the next 7 days after it was given treatment.. Anti-plasmodium activity test is determined by the number of parasitemia, percent growth and resistance of parasitemia in the blood. Data were analyzed by using one way anova method (? = 0,05 and 0,01). The results showed that the secondary metabolite compounds found in the ethyl acetate fraction of  P.canescens leaf, namely alkaloids, flavonoids, tannins and phenolics and at a dose of 0.056 g / KgBW  ethyl acetate fraction  can inhibit the growth of parasitemia in the blood of  M. musculus by 50 , 89% , with greater percentage of parasitemic inhibition compared with positive and negative control group.
UJI BIOFUEL HASIL PERENGKAHAN METIL ESTER DARI LIMBAH CAIR PABRIK MINYAK KELAPA SAWIT DENGAN KATALIS MONI/HZ Sari, Dian Kartika; Sundaryono, Agus; Handayani, Dewi
Alotrop Vol 1, No 2 (2017)
Publisher : FKIP University of Bengkulu

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[CONVERSION OF LIQUID WASTE OIL PALM OIL FACTORY BECOME BIOFUEL WITH MONi / HZ CATALYST] This study aims to produce biofuel from liquid waste oil PMKS with worditik cracking using catalystMoNi / HZ. This study involves the preparation of a catalyst performed by means of a separate impregnation, this impregnation lasting for 12 hours. The next stage of sample preparation includes heating, filtering, degumming, bleaching and ALB determination, followed by the production of methyl esters through esterification and transesterification processes. Then the obtained methyl ester was cracked using a catalyst for 2 hours at 380 °C and followed by distillation of the product for 2 hours at 380 °C, followed by distillation step at 380 °C to obtain methyl ester. The physical characteristics of biofuel resulting from the catalytic cracking reaction of the methyl ester using the MoNi / HZ catalyst of the PMKS wastewater include the acid number 1.92 mg KOH / g, density 0.803 g/cm3, kinematic viscosity 1,113 cSt, a pour poin of 0oC and fog poin at 1,43 oC. Biofuel resulting from catalytic cracking using this MoNi / HZ catalyst of very high biofuel acid numbers can be used in areas that have a cold climate. the biofuel viscosity of the experimental results has not matched the viscosity of gasoline or kerosene, so it can not be used as fuel equivalent to gasoline and kerosene with the composition of 100% biofuel density from biofuel is still slightly above gasoline and kerosene
PROFIL FITOKIMIA DAN PENGARUH EKSTRAK TANGKAI DAUN TALAS KEMUMU (COLOCASIA GIGANTEA HOOK.F ) TERHADAP JUMLAH LEUKOSIT MUS MUSCULUS Asri, Rofiqa; Handayani, Dewi; Sundaryono, Agus
Alotrop Vol 3, No 1 (2019)
Publisher : FKIP University of Bengkulu

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This research aims to identify secondary metabolite compounds in Talas kemumu leaf stalk (Colocasia gigantea Hook.f) and to determine the effect of extract on the number of male Mus musculus leukocytes that have been induced aspirin. Aspirin is used to increase the number of M.musculus leucocytes.The method used in this research is the method of extraction by maceration to attract secondary metabolites of active compounds  C.gigantea leaf stalk and the measurement of blood leukocyte counts are calculated using a hemocytometer (improved neubauer). Test of the effect of the leaf stalk of C.giganteaextract on the number leukocytes of M.musculus  was performed by dividing into 5 groups with 5 replications according to the Federer formula, namely K (-) were given distilled water, K (+) were given aspirin, P1, P2 and P3 are each given a dose of extract of  C.gigantea leaf stalk of 0,028 g/KgBB, 0,056 g/KgBB and 0,084 g/KgBB. The results showed that the identification of phytochemical profile in C.gigantea leaf stalk contains secondary metabolite compounds such as alkaloids, flavonoids, terpenoids, tannins and saponins. The results shows that giving of C.gigantea leaf stalk extract can reduce the number of leukocytes M.musculus which have induced aspirin. The giving of C.gigantea leaf stalk extract can decrease the number of aspirin-induced M.musculus leukocytes. This is evident from the results of One Way Anova analysis and the Smallest Real Difference Test (BNT) because in each treatment group has a significant influence. The effective dose that has the greatest activity in decreasing the number of M.musculus leukocytes found in the P1 treatment of 0,028 g /KgBB can decrease the blood leukocyte count by 6,830 / mm3.
SINTESIS METIL ESTER DARI LIMBAH KELAPA SAWIT DENGAN SONOCHEMISTRY DAN CRACKING MENJADI BIOFUEL DENGAN KATALIS CRMO/HZ Dhani, Jertadi Putri; Sundaryono, Agus; Handayani, Dewi
Alotrop Vol 3, No 2 (2019)
Publisher : FKIP University of Bengkulu

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This study aims to calculate the percentage methyl ester converted to biofuel through catalytic cracking reaction,analyzes component and determine characteristics using catalyst CrMo/HZ. This research begins with impregnation sample preparation which includes heating oil,degumming,bleaching and determination free fatty acid (FFA). Methyl ester obtained from liquid waste conversion crude palm oil through an esterification and transesterification reaction with H2SO4 and NaOH catalyst with sonochemistry methode is a method that utilizes ultrasonic waves origanating from a device is ultrasonic cleaner for one hour at temperature 60oC. The methyl ester obtained was cracked with a CrMo/HZ catalyst which was carried out for 2 hours at temperatur 380oC. To find out the components of compounds formed in methyl ester, an analysis was performed using GCMS then characteristics of biofuel are determined from the result of catalytic cracking obtained through acid number testing,density,viscosity,pour point and fog point. The result obtained from the conversion of methyl ester to biofuel with Crmo/HZ Catalyst Is 60,17% And For Testing The Physical Characteristics Of Biofuel Namely The Acid Number 1,87 Mg KOH/G, density 0,804 g/cm3, viscosity 1,162 Cst, pour point 0oC and fog point 1,4oC. The result of cracking produce a consituent component of biofuel namely gasoline 33,67%, kerosene 16,26%, gas oil 10,24% beside that, there are still methyl ester as much as21,89%.
PEMBUATAN NANOPARTIKEL-PERAK EKSTRAK DAUN UBI JALAR ORANGE (IPOMOEA BATATAS L.) DENGAN METODE BIOREDUKSI DAN UJI AKTIVITAS TERHADAP JUMLAH TROMBOSIT MUS MUSCULUS Trinanda, Ririn; Sundaryono, Agus; Handayani, Dewi
Alotrop Vol 3, No 1 (2019)
Publisher : FKIP University of Bengkulu

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This study aims to make silver nanoparticles (NPP) of orange Sweet potato extract (Ipomoea batatas L.) using bioreduction method and test the effect of giving silver nanoparticles - leaf extract of Ipomoea batatas L. orange (Ipomoea batatas L.) to the number of thrombocyte from male of  Mus musculus induced by aspirin. The precursor used is 1 mM AgNO3 solution and as bioreductor is an antioxidant compound in orange sweet potato extract (Ipomoea batatas L.). The ratio of AgNO3 1 mM solution volume and orange sweet potato leaf extract is 9: 1 with 2 hours time. The characteristic test again obtained NPP is done using FTIR and Particle Size Analyzer (PSA) using Dynamyc Light Scattering method (DLS). The results showed that from the PSA analysis is known the average size of NPP ? I. Batatas, L leaf extract  is an average of 86.0 nm with a polydispersity value (PDI) of 0.17 < 1 that indicates that the  NPP-leaf extract particle size distribution It is less homogeneous and are less stable.The results show that the administration of silver nanoparticles - orange sweet potato extract (Ipomoea batatas L.) can increase the platelet count of male mice significantly Dose of 0.014 g / Kgbb of 259,600 / mm3. 
UJI FITOKIMIA DAN TOKSISITAS EKSTRAK UMBI HYDNOPHYTUM SP. TERHADAP ARTEMIA SALINA LEACH Khasanah, Nur Wakidatul; Karyadi, Bhakti; Sundaryono, Agus
PendIPA Journal of Science Education Vol 4, No 1 (2020): FEBRUARY
Publisher : University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.4.1.47-53

Abstract

The goal of this research was to determine the content of secondary metabolites and the most active fraction of Simbagh Utak (Hydnophytum sp.) Tuber extracts. The content of secondary metabolites is determined by using phytochemical tests, and toxicity tests are carried out by the Brine Shrimp Lethality Test (BSLT) method. Hydnophytum bulbs from Jukung Village, Lubuklinggau City, South Sumatra were macerated using 96% ethanol and then evaporated until thick extracts were obtained. This thick extract was then tested for secondary metabolite content, fractionated using n-hexane, and ethyl acetate. The results of the fractionation were then carried out a toxicity test using the BSLT method. Secondary metabolite test results showed ethanol extract containing flavonoids, alkaloids, tannins, saponins, terpenoids, and phenolics. The result of fractionation was obtained by the tuber extract of Hydnophytum n-hexane fraction, ethyl acetate fraction, and ethanol fraction. Toxicity test results obtained LC50 values of n-hexane fraction of 52.3 ppm, LC50 of ethyl acetate fraction of 45.9 ppm, and LC50 of ethanol fraction of 99 ppm. All Hydnophytum tuber extract fractions were categorized as toxic and have potential as anti-bacterial or anti-oxidant. The fraction that has the lowest LC50 price is the most toxic fraction. The most toxic fraction is the most active fraction. The ethyl acetate fraction was the most active fraction because it has the lowest LC50.
Development of The Stem of Jatropha multifida Linn as A New Antimalarial through Erythrocytes Test on Mus musculus Infected by Plasmodium berghei Sundaryono, Agus; Ruyani, Aceng; Sari, Rika Partika
Journal of Biomedicine and Translational Research Vol 1, No 1 (2015): April 2015
Publisher : Faculty of Medicine, Diponegoro University

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Introduction : Jatropha multifida Linne is known as Betadin plant by the Bengkulunese. The stem extract of J. multifida has antibacterial activity. This study examined the potential of stem extract of J. multifida to be developed as an anti-malarial drugs through trials in M. musculus whose erythrocytes infected by Plasmodium berghei.Methods : Phytochemical test of the stem of J. multifida to test the flavonoids, alkaloids, tannins, saponins, terpenoids and steroids. The stem extract of J. multifida obtained by maceration with 96% ethanol. 30 M. musculus divided 6 groups, each consist of 5 animals. P0 is the negative control groups that were not infected by P berghei. P1 is the positive control groups that were only infected by P berghei, P2 is a comparison groups that were infected with P berghei and chloroquine, groups P3, P4, and P5 were infected by P berghei and treated with stem extract of J multifida with consecutive doses 0.028 g / kgbw, 0.056 g / kgbw and 0.084 g / kgbw. After 24 hours, the number of erythrocytes was observed with hemocytometer then was counted the number of uninfected erythrocytes by P berghei using a microscope at 1000x magnification.Results :The stem extract of J. multifida at doses of 0.028 g/kgbw, 0.056 g/kgbw and a dose of 0.084 g/kgbw were able to increase the number of uninfected erythrocytes by P berghei in M. musculus, respectively an average of 9.135million cell eritrosit/mm3, 7.618 millioncell eritrosit/mm3, and 9.856 millioncell eritrosit/mm3. The ability of stem extract of J. multifida in increasing the number of erythrocytes uninfected with P berghei was much higher than the malaria drug chloroquine diposphat. On one way ANOVA analysis of Fcount ( 13,2 ) &gt; Ftable (2.76 ) , with (α = 0.05), there are noticeable differences in the provision of treatment. The increasing number of uninfected erythrocytes by P berghei was due to the content of flavanol glycosides in J multifida stem. Flavanol glycosides was expected to form Flvanolglycosides-heme complex that could inhibit the formation of P berghei parasites HemazoinConclusion:The stem extract of J. multifida could be developed as a potential anti-malarial drugs since it could increase the number of uninfected erythrocytes by P berghei in M. musculus. Its ability was much higher than the malaria drug chloroquine diposphat
ENVIRONMENTAL CHEMISTRY MODULES DEVELOPMENT TO ENHANCE STUDENTS' CREATIVE THINKING ABILITIES IN HEAVY METAL WATER POLLUTION MATERIALS Apriyoanda, Hadi; Sundaryono, Agus; Firdaus, M. Lutfi
PendIPA Journal of Science Education Vol 4, No 1 (2020): FEBRUARY
Publisher : University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.4.1.1-6

Abstract

Development of teaching material based on laboratory research is one way that can be done to improve students' creative thinking abilities in meeting the challenges of 21st century learning. The aims of this study are to determine the feasibility of the developed environmental chemistry modules, to improve of students' thinking abilities and determine the students? responses to the use of modules in environmental chemistry lectures. Module development by R and D methods 4D model, namely Define, Design, Develop and Disseminate. Module quality is determined by expert validation test. Based on the results of expert validation, the module developed obtained 90.91% percentage of eligibility with a very good category. Based on the N-Gain value obtained, an increase in students' creative thinking abilities on the aspect of  flexibility was 0.47, and the aspect of elaboration was 0.64 with medium category. The student responses to the use of modules in learning enviromental chemistry obtained a percentage of 83.82% with very good category.
UJI AKTIVITAS ANTIPLASMODIUM FRAKSI N-HEKSANA DAUN Peronema canescens TERHADAP Mus musculus Andriani, Fenny; Sundaryono, Agus; Nurhamidah, Nurhamidah
Alotrop Vol 1, No 1 (2017)
Publisher : FKIP University of Bengkulu

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Peronema canescens (Sungkai) has been used in Bengkulu as raw herbal remedy to reduce the fever, some people are using as a malaria drug. P.  canescens leaves contain alkaloids, flavonoids, tannins and terpenoids – steroids .This study aims to analyze the effect of P.  canescens leaves  n-hexane fraction.  against paracetemias in Mus musculus infected with Plasmodium berghei, then to prove whether the . P.  canescens leaves n-hexane fraction  has potential as alternative medicine for malaria .   To make P. canescens extract , leaves was macerated using EtOH (96%), then filtrate was evaporated using a rotary evaporator, then fractionated with n-hexane. The 25 healthy  M.musculus  weighing 20-40g each, infected with P.berghei, grouped into 5 ie group 1 (K-) was treated orally with aquades, group 2 (K +) was treated orally with 0.42 mg of Chloroquine, Group 3 (P1) Group 4 (P2), group 5 (P3) was treated orally with a P.  canescens leaves n-hexane fraction  each at a dose of 0.028 ,  0.056 and 0.084 g / kgBW. After 3 days of digestion for each treatment, the amount of erythrocytes was calculated under a microscope. : The giving of the with dose 0,028 g / kgBW able to inhibit paracetemia 33,49%,  0,056 g / kgBW able to  57.91% and  0,084 g / kgBW able to  61.69%   The conclusion is the P. canescens leaves n-hexane fraction  at a dose of 0.028 , 0.056 , and 0.084 g / kgBW orally wil be decreased the amount of paracetemia in M.musculus, and the higher the dose given the higher percent of the inhibition.