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Journal : Jurnal Kedokteran Brawijaya

EKSPRESI CYTOSOLIC ASPARTATE-SPECIFIC CYSTEINE PROTEASE-3 (CASPASE-3) PADA JARINGAN HATI RATTUS NORVEGICUS (WISTAR) SETELAH PEMBERIAN SUBKRONIK AFLATOKSIN B1 (AFB1) Maritha, Indah Dina; Supranowo, Supranowo; Lyrawati, Diana
Jurnal Kedokteran Brawijaya Vol 22, No 3 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2006.022.03.4

Abstract

Aflatoksin B1(AFB1) is one of the toxic agents produced by Aspergillus flavus that frequently contaminates foods not properly stored. AFB1 undergoes biotransformation which may result in the production of reactive oxygenspecies (ROS) hazardous to liver cells. Following a cascade of oxidative reaction, ROS will cause the mitochondria torelease cytochrome c which subsequently activates caspase-3, leading to apoptosis. In this present study we evaluated the effect of aflatoxin B1 on the expression of caspase-3 in the liver. AFB1 was administered per oral, at different dosage and length of exposure, subchronically. This study was carried out as a factorial designed experiment with  two factors. The first was dosage factor i.e 0, 10, 15 and 20 µg (0; 0,05; 0,075; 0,1 µg/g BW) and the second was exposure time factor i.e. 12, 16 and 20 weeks. Rattus norvegicus strain Wistar aged approximately eight weeks old and weighed 180-200 g were used as the experimental animals. The expression of  caspase-3 was examined by using immunohistochemistry. The results showed that the expression of caspase-3 increased significantly (p = 0,000) with the escalation of AFB1 in dosage and/or exposure time (p = 0,001). In the interaction between dose and exposure time of AFB1an increase in the expression of caspase-3 was also observed (p = 0,000). Interestingly, these studies also revealed thatin the liver tissues there was a limitation in the expression of caspase-3, where the raising of further AFB1 dosage and length of exposure were not followed by further increase of the caspase-3.
EKSTRAK ROSELA MENURUNKAN PERLEMAKAN DAN EKSPRESI ADMA HEPAR AKIBAT DIET ATEROGENIK PADA TIKUS Fathoni, Zuhrotun Ulya; Indra, Rasjad; Supranowo, Supranowo
Jurnal Kedokteran Brawijaya Vol 28, No 1 (2014)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2014.028.01.2

Abstract

Rosela memiliki anthocyanin sebagai antioksidan polifenol yang berpotensi mencegah progresivitas penyakit akibat stres oksidasi. Diet aterogenik dan stres oksidasi memicu serangan radikal bebas pada komponen lemak dan protein sehingga terjadi perlemakan hepar dan peningkatan ekspresi ADMA (Asymmetric dimethylarginine). Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak rosela strain Hibiscus sabdariffa terhadap perlemakan hepar dan ekspresi ADMA hepar pada tikus Rattus norvegicus yang diberi diet aterogenik. Studi eksperimental menggunakan post test only control group pada Rattus norvegicus strain Wistar jantan yang dibagi dalam empat kelompok, yaitu kelompok kontrol positif (diet aterogenik), kelompok1 (ekstrak rosela 150mg/kgBB/hari + diet aterogenik), kelompok 2 (ekstrak rosela 300mg/kgBB/hari + diet aterogenik), dan kelompok kontrol negatif (tanpa pemberian diet aterogenik dan ekstrak rosela). Hasil penelitian menunjukkan bahwa ekstrak rosela mampu menurunkan perlemakan hepar yang berdasar pemeriksaan histopatologi berupa jumlah sel yang mengalami perlemakan lebih sedikit (p=0,000). Ekspresi ADMA dari gambaran imunohistokimia menunjukkan ekspresi yang lebih sedikit pada kelompok dengan diberi ekstrak rosela (p=0,000). Dapat disimpulkan pemberian ekstrak rosela menghambat perlemakan hepar akibat diet aterogenik dan ekspresi ADMA akibat diet aterogenik pada tikus, dengan dosis optimum rosela 300 mg/kgBB/hari. Kata Kunci: Diet aterogenik, ekspresi ADMA hepar, perlemakan hepar, rosela