Articles

7-HIDROKSI-6-METOKSI KUMARIN (SKOPOLETIN) DARI KULIT BATANG CHISOCHETON CELEBICUS (MELIACEAE) Katja, Dewa G.; Sonda, Andre A.; Huspa, Desi Harneti P.; Mayanti, Tri; Supratman, Unang
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

7-hydroxy-6-methoxy coumarin (scopoletin) has been isolated from the bark of Chisocheton celebicus (Meliacee) using several chromatographic techniques.  The chemical structure of 7-hydroxy-6-methoxy coumarin was identified on the basis of spectroscopic data including UV, IR, 1D-NMR, 2D-NMR and mass along with by comparison with those spectral data previously reported. The discovery of scopoletin from the bark C. celebicus reported for the first time in this study.
AN ISOFLOVONOID, WARANGALONE FROM THE STEM BARK OF DADAP AYAM (ERYTHRINA VARIEGATA) Herlina, Tati; Nasrudin, Nasrudin; Supratman, Unang; Subarnas, Anas; Sutardjo, Supriyatna; Hayashi, Hideo
Jurnal ILMU DASAR Vol 9 No 1 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

In the course of our continuing search for novel paralytic compound from Indonesian plants, the methanol extract of the stem bark of Erythrina variegata (Leguminosae) showed significant paralytic activity against the third instar larvae of silkworm (Bombyx mori). The purposes of this research were isolation and structural elucidation of paralytic compound from the stem bark of E. variegata. Using the paralytic activity following the separation, the methanol extract was separated by combination of column chromatography to yield prenylisoflavone, warangalone. The chemical structure of warangalone was identified based on spectroscopic evidence and comparison with the previous reported. The paralytic activity of warangalone showed weak activity against the third instar larvae of silkworm (B. mori).
SENYAWA FENOLIK DARI DAUN TANAMAN KALANCHOE PROLIFERA (CRASSULACEAE) Yun, Yenny Febriani; Aisyah, Lilis Siti; Saputra, Tri Reksa; Hakim, Arif Rahman; Purbaya, Sari; Herlina, Tati; Julaeha, Euis; Zainuddin, Achmad; Supratman, Unang
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 3, No. 1, Mei 2017
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (582.388 KB) | DOI: 10.15408/jkv.v3i1.5037

Abstract

Phenolic compounds such as kaempferol (1), quercetin (2), and methyl caffeate (3) have been isolated from the ethyl acetate extract of Kalanchoe prolifera (Crassulaceae). The chemical structure of isolated compounds 1-3 were elucidated on the basis of spectroscopic evidence (UV, IR, NMR) and comparison with those compound previouly reported.DOI: http://dx.doi.org/10.15408/jkv.v0i0.5037 
STIGMAST-5(6)-EN-3(6)-OL DARI HERBA TUMBUHAN KROKOT (LYGODIUM MICROPHYLLUM) Kuncoro, Hadi; Farabi, Kindi; Julaeha, Euis; Rijai, Laode; Supratman, Unang
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 1, No. 1, Mei 2015
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

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Abstract

A steroid, stigma-5(6)-en-3?-ol or ?-sitosterol (1) had been isolated from a n-hexane extract of the Krokot plant herb (Lygodium microphyllum). The chemical structure of 1 was identified on the basis of spectroscopic evidences and by comparison with those of spectra data previously reported. The investigation of steroid, stigma-5(6)-en-3?-ol was from L. microphyllum plant was reported inthis research for first time.DOI :http://dx.doi.org/10.15408/jkv.v0i0.3152
CYTOTOXIC STEROIDS FROM THE STEMBAK OF CHISOCHETON CELEBICUS KOORD Katja, Dewa Gede; Harneti, Desi; Mayanti, Tri; Nurlelasari, Nurlelasari; Maharani, Rani; Shiono, Yoshihito; Supratman, Unang
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 5, No. 2, November 2019
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.577 KB) | DOI: 10.15408/jkv.v5i2.12132

Abstract

In the course of our continuing search for anticancer compounds from Chisocheton species, three steroids, stigmast-5-en-3?-ol (1), stigmast-5-en-3?-ol-3-O-?-D-glucopyranoside (2) and stigmast-5,22-dien-3?-ol-3-O-?-D-glucopyranoside (3), were obtained from the stembark of Chisocheton celebicus. The structures of compound 1-3 were identified with spectroscopic data including IR, 1D-NMR, 2D-NMR and TOF-MS, as well as by comparing with those spectral data previously. Compounds 1-3, were evaluated for their cytotoxic effects against P-388 murine leukemia cells and displayed the cytotoxicity activity with IC50 values of 12.45 ± 0.050, 52.27 ± 0.031 and 62.52 ± 0.076 µg/mL, respectively.
SINTESIS PEPTIDA P251-9 BERGUGUS PELINDUNG DENGAN METODE SINTESIS PEPTIDA FASA PADAT FMOC/TBU MENGGUNAKAN ADITIF OKSIMA Hardianto, Ari; Subroto, Toto; Supratman, Unang
Jurnal Sains dan Terapan Kimia Vol 5, No 2 (2011)
Publisher : Jurnal Sains dan Terapan Kimia

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Abstract

Peptida P25 merupakan epitop yang dapat dengan mudah mengaktivasisel T penolong. Sel T penolong teraktivasi berperan dalam aktivasi sel Buntuk membentuk antibodi. Peptida bahkan protein dapat dibuat denganmetode sintesis peptida fasa padat Fmoc/tBu menggunakan aditif HOBt.Namun sayangnya HOBt memiliki karakter yang mudah meledak. Padapenelitian ini telah berhasil disintesis bagian epitop P25 (peptida P251-9bergugus pelindung) yang memiliki tingkat kemurnian tinggi dan perolehan36,4% dengan metode sintesis peptida fasa padat Fmoc/tBu menggunakanaditif oksima pengganti HOBt.Kata kunci: Epitop, P251-9, sintesis peptida fasa padat Fmoc/tBu, oksima.
INSECTICIDAL BUFADIENOLIDES FROM THE LEAVES OF KALANCHOE DAIGREMONTIANA (CRASSULACEAE) Hermawan, Wawan; Maharani, Rani; Fajriah, Sofa; Hardiawan, Revan; Supratman, Unang
Jurnal ILMU DASAR Vol 11 No 2 (2010)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Kalanchoe is the biggest genera of Crassulaceae family and distributed in tropical and subtropical regions. This genera is found to be a rich source of biologically active natural products such as triterpenes, flavonoids and steroids. As a part of our continuing search for novel insecticidal compounds from Indonesian Kalanchoe plants, we examined Kalanchoe daigremontiana collected from Bandung region, West Java, Indonesia. The methanolic extract of the dried leaves of K. daigremontiana was concentrated and extracted with methylene chloride The methylene chloride extract exhibited an insecticidal activity toward silkworms. The methylene chloride extract was partitioned between n-hexane and methanol containing 10% water. The active lower layer was extracted with ethyl acetate. By using the insecticidal activity to follow the separations, the ethyl acetate fraction was separated by combination of column chromatography on Kieselgel 60 and ODS to afford two insecticidal bufadienolides 1 and 2. The structures of these compounds were elucidated based on spectroscopic analysis (UV, IR, NMR, 2D-NMR) and comparison with those related data previously reported. In this paper, the isolation, structural elucidation, and insecticidal activities against the third instar larvae of silkworm will be described.
SENYAWA DAMARA-20,24-DIEN-3-ON YANG BERAKTIVITAS SITOTOKSIS TERHADAP SEL MCF-7 DARI KULIT BATANG CHISOCHETON MARCROPHYLLUS (MELIACEAE) Nurlelasari, Nurlelasari; Ferdiana, Nurabi; Mauluddin, Subekti; Harneti, Desi; Moektiwardoyo, Moelyono; Abdullah, Rizky; Supratman, Unang; Awang, Khalijah
Indonesian Journal of Applied Sciences Vol 5, No 3 (2015)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijas.v5i3.15062

Abstract

AbstrakSenyawa triterpenoid damaran, damara-20,24-dien-3-on telah diisolasi dari kulit batang Chisocheton Marcrophyllus (Meliaceae). Struktur kimia kedua senyawa tersebut telah ditetapkan berdasarkan data spektroskopi dan perbandingan data spektra yang diperoleh dari literatur. Senyawa damara-20,24-dien-3-on menunjukkan aktivitas sitotoksik terhadap sel kanker payudara manusia MCF-7 dengan nilai IC50 28 mM.Kata Kunci : aktivitas sitotoksik, sel MCF-7, triterpenoid damaran, Chisocheton Marcrophyllus,  MeliaceaeAbstractDammarane triterpenoid, damara-20,24-dien-3-on has been isolated from the bark of Chisocheton Marcrophyllus (Meliaceae). Chemical Structure of compound was dtermined on the basic of spektroscopic data and comparison with those spectra data previously reported. Compound damara-20,24-dien-3-on exhibited cytotoxic activity againts MCF-7 human breast cancer cell lines with IC50 value 28 mM respectively.Kata Kunci : cytotoxic activity, MCF-7 cell, dammarane triterpenoid, Chisocheton Marcrophyllus,  Meliaceae
PERAN METABOLIT SEKUNDER DARI DAUN RASAMALA (ALTINGIA EXCELSA NORNHA) SEBAGAI PENGHAMBAT SIKLUS SEL DAN INDUKSI APOPTOSIS SEL KANKER LIDAH MANUSIA IN VITRO Anwar, Risyandi; Setiawan, Arlette; Supratman, Unang; Supriatno, Supriatno
Indonesian Journal of Applied Sciences Vol 5, No 2 (2015)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijas.v5i2.16662

Abstract

AbstrakKanker lidah merupakan suatu keganasan yang sering terjadi pada rongga mulut dan  merupakan penyakit dengan karakteristik pertumbuhan sel yang agresif, dengan prognosis buruk, dan seringkali menimbulkan kematian. Oleh karena itu, diperlukan perawatan yang cepat, tepat, efektif dengan efek samping minimal. Pengobatan herbal merupakan salah satu jawabannya karena metabolit sekunder dari bahan alam telah diakui mempunyai potensi sebagai antikanker. Tumbuhan famili Hammamedaceae sudah banyak diteliti dan menghasilkan senyawa yang berpotensi sebagai antikanker. Pada penelitian ini metabolit sekunder tumbuhan Rasamala (Altingia excelsa Nornha) yang merupakan salah satu tumbuhan famili Hammamelidaceae diinkubasi dengan kanker lidah manusia (SP-C1). Penelitian bertujuan untuk mengungkapkan metabolit sekunder dari daun Rasamala, menguji aktivitasnya sebagai penghambat siklus sel melalui hambatan siklus sel, penekanan ekspresi proto-onkogen c-myc, dan induksi apoptosis dengan jalan meningkatkan aktivitas proteolitik kaspase-8 dan kaspase-9.  Penelitian dilakukan dengan cara eksperimental laboratorik menggunakan sel kanker lidah manusia SP-C1. Daun kering Rasamala diekstraksi dengan metanol pada temperatur kamar dan ekstrak metanol yang diperoleh diuapkan pelarutnya pada tekanan rendah dihasilkan ekstrak metanol pekat (254 g). Ekstrak metanol pekat dilarutkan dengan air dan dipartisi berturutturut dengan n-heksana dan etil asetat. Semua ekstrak yang diperoleh diuji aktivitas antiproliferasi terhadap sel kanker lidah SPC1 secara in vitro, dan ekstrak etil asetat menunjukkan aktivitas anti proliferasi yang tertinggi. Ekstrak etil asetat dipisahkan senyawa metabolit sekundernya dengan berbagai teknik kromatografi yang dipandu dengan uji antiproliferasi dihasilkan senyawa 1-5. Struktur kimia senyawa 1-5 diidentifikasikan berdasarkan metode spektroskopi meliputi UV-Vis, IR, 1H NMR, dan 13C NMR dan diidentifikasikan sebagai asam-3,4-dihidroksi benzoat (1), asam galat (2), apigenin (3), kaempferol (4), dan kuersetin (5). Senyawa 1-5 diuji aktivitas antiproliferasi terhadap sel kanker lidah SP-C1 dan menunjukkan nilai IC50 berturutturut  100,  100,  100, 0,72 dan 0,70 mg/mL. Analisis pertumbuhan sel menggunakan MTT assay dilakukan untuk menguji pengaruh metabolit sekunder dari daun Rasamala terhadap pertumbuhan sel SP-C1. Analisis Flowcytometri dilakukan untuk melihat hambatan siklus sel SP-C1 yang diberi perlakuan kaempferol (4) dan kuersetin (5). Pengujian apoptosis dilakukan melalui analisis aktivitas kaspase-8 dan kaspase-9.  Penekanan ekspresi protein c-myc dilakukan melalui analisis Western blotting. Analisis data menggunakan uji ANACOVA, Tukey HSD dengan tingkat kemaknaan ?=0,05. Kaempferol (4) dan kuersetin (5) menghambat siklus sel pada fase G0-G1 dengan cara menekan ekspresi c-myc dan meningkatkan apoptosis dengan cara meningkatkan aktivitas kaspase-8 dan kaspase-9. Kesimpulan dari penelitian ini adalah metabolit sekunder dari daun Rasamala, yaitu kaempferol (4) dan kuersetin (5) memiliki aktivitas sebagai antikanker lidah manusia SP-C1 melalui hambatan siklus sel, menekan ekspresi c-myc dan menginduksi apoptosis...Kata kunci: Altingia excelsa, siklus sel, sel kanker lidah SP-C1, apoptosis, kaempferol, kuersetin.AbstractTongue cancer is a common malignant in the oral cavity and it has characterized with the aggressive cell growth, poor prognosis and often treated of life. For that reason, effective treatment with minimum side effect, accurate and easy to be found are needed. Herbal medicine is one of the answer because its secondary matabolities are believed to have an anticancer activity. Anticancer activity of Hammamelidaceae family has been reported. In the present study, secondary matabolities of Rasamala leaves (Altingia excelsa Nornha) is one of plant belongs to Hammamelidaceae family, was incubated within the human oral tongue cancer cell (SP-C1). The aims of study were to identify the secondary matabolities of Rasamala leaves, to examine the secondary matabolities of Rasamala leaves toward SP-C1 cell growth inhibition through analysis of cell cycle arrest, suppression of proto-oncogen c-myc expression, and induction of apoptosis via increased proteolityc activity of caspase-8 and -9. True experimental laboratories design using SP-C1 oral tongue cancer cell line was used. The dried leaves of Rasamala was extracted with methanol at room temperature and evaporated at reduced pressure resulting concentrated methanol extract (254 g). The concentrated methanol extract was dissolved in water and successively partitioned with n-hexane and ethyl acetate. All of the extracts was tested on their antiproliferative activity against tongue cancer cells SP-C1 in vitro, and the ethyl acetate extracts showed strongest antiproliferative activity. The ethyl acetate extract was separated their secondary metabolites by various chromatographic techniques guided by antiproliferasi activity to yield compounds 1-5. The chemical structure of compounds 1-5 were identified by spectroscopic methods including UV-Vis, IR, 1H NMR and 13C NMR and identified as 3,4-dihydroxy benzoic acid (1), gallic acid (2), apigenin (3), kaempferol (4), and quercetin (5). Compounds 1-5 were tested on their antiproliferative activity against cancer cells tongue SP-C1 and showed IC50 values of > 100, > 100, > 100, 0.72 and 0.70 mg/mL, respectively. Cell growth analysis using MTT assay was performed to examine the effect of  secondary metabolities of Rasamala leaves toward SPC1 cell growth. Flowcytometry analysis was delivered to test the cell cycle arrest of SP-C1 cell treated by kaempferol (4) and quercetin (5). Apoptosis assay was confirmed by caspase-8 and -9. The suppression of c-myc protein was done by Western blotting analysis. Data analysis using ANACOVA assay, Tukey HSD with the level of significance ?=0.05. Kaempferol (4) and quercetine (5) were markedly induced the cell cycle arrest in G0-G1 phase through the suppression of c-myc expression and increased apoptosis via up-regulation of caspase-8 and -9. In conclusion, secondary metabolites of Rasamala leaves, kaempferol (4) and quercetin (5) have a strong anticancer activity in human oral tongue cancer SP-C1 through induction of cell cycle arrest, suppression of c-mcy protein expression and induction of apoptosis.Keywords: Altingia excelsa, cell cycle, human oral tongue cancer SP-C1, apoptosis, kaempferol, quercetin.
SENYAWA BUFADIENOLIDA YANG BERSIFAT INSEKTISIDA, DAIGREMONTIANIN DARI DAUN COCOR BEBEK (KALANCHOE DAIGREMONTIANA) Mayanti, Tri; Huspa, Desi Harneti Putri; Nurlelasari, Nurlelasari; Safari, Agus; Supratman, Unang
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.2, Mei 2011
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.131 KB) | DOI: 10.15408/jkv.v2i2.200

Abstract

Dalam penelitian berkelanjutan untuk pencarian senyawa-senyawa yang bersifat insektisida daritanaman Kalanchoe (Cocor bebek) Indonesia, diperoleh hasil bahwa ekstrak metanol daun Cocorbebek (Kalanchoe daigremontiana) memberikan aktivitas insektisida yang kuat terhadap instar ke tigalarva ulat sutera (Bombyx mori). Ekstrak metanol yang diperoleh selanjutnya dipisahkan senyawasenyawanyamenggunakan partisi dengan pelarut organik dan kombinasi kolom kromatografi padasilika gel dan ODS sehingga dihasilkan satu senyawa aktif insektisida. Struktur kimia senyawa yangbersifat insektisida tersebut ditentukan dengan metoda spektroskopi dan perbandingan data spektrasenyawa analog yang telah diperoleh dari penelitian sebelumnya sehingga senyawa aktif insektisidatersebut diidentifikasikan sebagai senyawa turunan bufadienolida, daigremontianin. Daegremontianinmenunjukkan aktivitas insektisidal yang kuat terhadap instar ke tiga ulat sutera (Bombyx mori) denganLD50 0,9 ?g/g diet.
Co-Authors . Susianti Abdullah, Rizky Ace Tatang Hidayat Achmad Zainuddin Ade Kania Ningsih, Ade Kania Adel Zamri Agus Safari Amir M Suruwaky Anas Subarnas Anas Urbanas Andre A. Sonda, Andre A. Anwar, Risyandi Apriani, Riza Ari Hardianto Ari Widiyantoro Arif Rahman Hakim Arlette Setiawan, Arlette Awang, Khadijah Azmi, Mohamad Nurul Budiyansyah, Teddy CHRISTOFORA HANNY WIJAYA Dadan Sumiarsa Danar Dono Darwati Darwati Desi Harneti P. Huspa, Desi Harneti P. Desi Harneti Putri Huspa Destiarti, Lia Dewa G. Katja Dewa Gede Katja Dikdik Kurnia DONDIN SAJUTHI Edi Suanto, Edi Edi Sukmana Elvi Rusmiyanto, Elvi Euis Julaeha Faizal Hermanto, Faizal Fajar Fauzi Abdullah Fajar, Mohamad Fajar, Mohamad Farabi, Kindi Farabi, Kindi Farida, Ida Nur Ferdiana, Nurabi Goenawan, Hana Hadi Kuncoro Haikal, Kansy Haikal, Kansy Haraswati, Nayla Harlia, Harlia Harnet, Desi Harneti, Desi Harneti, Desi Hendra, Rudi Hideo Hayashi Horizon, . Husein Hernandi Bahti Indriyani, Indri Indriyani, Indri Inne Suherna Sasmita Kadarusman, Kadarusman Khalijah Awang Krismayanti, Elisabeth Laode Rijai Lesmana, Ronny Lilis Siti Aisyah, Lilis Siti Lindung Tri Puspasari Mardianingrum, Richa Mardianingrum, Richa Marpaung, Christina Maryati Maryati Mauluddin, Subekti Miftah, Nur Muhammad Milawati, Hersa Milawati, Hersa Moelyono Moektiwardoyo, Moelyono Murtihapsari Murtihapsari Nasrudin Nasrudin Nishinarizki, Vicki Noor Rain Abdullah Nunung Kurniasih, Nunung Nurfarida, Ida Nurlelasari Nurlelasari Octavia, Siska Mulya Paramita, Hedi Paramita, Hedi PH, Desi Harneti Ph.D, Rani Maharani Ponis Tarigan Pujiastuti, Betry Purbaya, Sari Puspita Sari Putri Huspa, Desi Harneti Putri Huspa, Desi Harneti Rahim, Andi Rahmawati Rahmawati Rahmi, Jihan Mudrika Rani Maharani Ratu Safitri Rayapratiwi, Witriany Revan Hardiawan RIKA MELIANSYAH Rizky Abdulah, Rizky Rizqullah, Herdanu Roosje Rosita Oewen, Roosje Rosita Rymond J. Rumampuk S. Supriatno, S. Satwika Nandiwardhana Setiawan Setiawan Shabarni Gaffar, Shabarni Shiono, Yoshihito Shiono, Yoshihito Soedjanaatmadja, M. S. Sofa Fajriah Sukmawati, Winda Supriatno Supriatno Supriyatna Supriyatna Supriyatna Sutardjo Supriyatna, - Suseno Amien Tati Herlina Taylor, W.C. Thufaila, Nadya Toto Subroto Tri Mayanti Tri Reksa Saputra, Tri Reksa Tsamrotul, Alya Vita Murniati Tarawan Wahyuni, Aneu Wahyuni, Aneu Wawan Hermawan Wismandanu, Okta Yenny Febriani Yun, Yenny Febriani Yum Eryanti Yusup Hidayat