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PEMBERIAN INSEKTISIDA ALAMI DARI EKSTRAK NANOEMULSI DAUN KETUMPANG (TRIDAX PROCUMBENS L.) UNTUK PENGENDALIAN PERILAKU DAN KEMATIAN ULAT KROP (CROCIDOLOMIA PAVONANA F.) PADATANAMAN SAWI Safrida, Safrida; Wulandari, Noor Aisah Riski; Supriatno, Supriatno
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.2.199

Abstract

Ketumpang (Tridax procumbens L.) contains bioactive compounds that can be used as natural insecticides. To reduce particle size, the extracts were prepared using nanoemulsion techniques. This study aims to determine the effect of nanoemulsion of intercropping leaf extract on behavior and mortality of crop caterpillars on mustard plants. This study used an experimental method with a completely randomized design (CRD) consisting of seven treatments and four replications. The treatments consisted of, K- = Administration of nanoemulsion extract at a concentration of 0% (K-), P1 = Administration of nanoemulsion extract at a concentration of 5%, P2 = Administration of nanoemulsion extract at a concentration of 10%, P3 = Administration of nanoemulsion extract at a concentration of 15 %, P4 = Administration of nanoemulsion extract at a concentration of 20%, P5 = Administration of nanoemulsion extract at a concentration of 25%, and K + = Administration of the chemical insecticide deltamethrin at a concentration of 0.04% as a positive control. Data were analyzed using descriptive and analysis of variance, as well as further tests at ?=0.05. The results showed that administration of nanoemulsion ketumpang leaf (Tridax procumbens L.) extract caused behavioral changes (eating, resting, and moving) of caterpillar crop which became inactive and increased mortality of crop caterpillar. Nanoemulsion extracts of overlap leaves could be used to control crop caterpillars. Keywords: crop caterpillars, ketumpang leaf, mustard, nanonemulsion
In vivo gene therapy with plasmid DNA-Tuberous sclerosis complex-2 (TSC-2) in an oral squamous cell carcinoma Supriatno, Supriatno
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 37, No 02 (2005)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Tuberous sclerosis complex (TSC) is an inherited syndrome in which affected individuals are at increased risk for developing benign tumors. Mutation of the TSC-2 gene encoding tuberin on chromo-some 16p13.3 give rise to the clinical disorder of tuberous sclerosis characterized by the development of hamartomas.Objectives: In the present study, I conducted to examine whether overexpression of TSC-2 can affect the growth of human oral squamous cell carcinoma (OSCC) which have different expression level of p27KP. Methods: I constructed an expression vector containing sense-oriented rat TSC-2 cDNA with pcDNA3.1 (Invitrogen), and transfected to oral squamous cell carcinoma (B88) to regulate the expression of TSC-2 gene in each transfectant. Western blot analysis was carried out to determine the protein level of TSC-2. Therefore, Tumor volume and body weight of nude mice was examined using tumorigenicity assay.Results: Overexpression of TSC-2 exerted the growth inhibitory effect of oral squamous cell carcinoma. Sense-oriented B88-TSC-2 cancer cells, have high expression of p27KP1. Moreover, tumor induced by TSC2 up-regulated transfectans become much smaller than those of control cells.Conclusion: overexpression of TSC-2 may exert the antitumor effect on oral squamous cell carcinoma through p27KP induction. Key word: TSC-2 - transfection - oral squamous cell carcinoma - pcDNA3.1 - mutation. 
PERAN METABOLIT SEKUNDER DARI DAUN RASAMALA (ALTINGIA EXCELSA NORNHA) SEBAGAI PENGHAMBAT SIKLUS SEL DAN INDUKSI APOPTOSIS SEL KANKER LIDAH MANUSIA IN VITRO Anwar, Risyandi; Setiawan, Arlette; Supratman, Unang; Supriatno, Supriatno
Indonesian Journal of Applied Sciences Vol 5, No 2 (2015)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijas.v5i2.16662

Abstract

AbstrakKanker lidah merupakan suatu keganasan yang sering terjadi pada rongga mulut dan  merupakan penyakit dengan karakteristik pertumbuhan sel yang agresif, dengan prognosis buruk, dan seringkali menimbulkan kematian. Oleh karena itu, diperlukan perawatan yang cepat, tepat, efektif dengan efek samping minimal. Pengobatan herbal merupakan salah satu jawabannya karena metabolit sekunder dari bahan alam telah diakui mempunyai potensi sebagai antikanker. Tumbuhan famili Hammamedaceae sudah banyak diteliti dan menghasilkan senyawa yang berpotensi sebagai antikanker. Pada penelitian ini metabolit sekunder tumbuhan Rasamala (Altingia excelsa Nornha) yang merupakan salah satu tumbuhan famili Hammamelidaceae diinkubasi dengan kanker lidah manusia (SP-C1). Penelitian bertujuan untuk mengungkapkan metabolit sekunder dari daun Rasamala, menguji aktivitasnya sebagai penghambat siklus sel melalui hambatan siklus sel, penekanan ekspresi proto-onkogen c-myc, dan induksi apoptosis dengan jalan meningkatkan aktivitas proteolitik kaspase-8 dan kaspase-9.  Penelitian dilakukan dengan cara eksperimental laboratorik menggunakan sel kanker lidah manusia SP-C1. Daun kering Rasamala diekstraksi dengan metanol pada temperatur kamar dan ekstrak metanol yang diperoleh diuapkan pelarutnya pada tekanan rendah dihasilkan ekstrak metanol pekat (254 g). Ekstrak metanol pekat dilarutkan dengan air dan dipartisi berturutturut dengan n-heksana dan etil asetat. Semua ekstrak yang diperoleh diuji aktivitas antiproliferasi terhadap sel kanker lidah SPC1 secara in vitro, dan ekstrak etil asetat menunjukkan aktivitas anti proliferasi yang tertinggi. Ekstrak etil asetat dipisahkan senyawa metabolit sekundernya dengan berbagai teknik kromatografi yang dipandu dengan uji antiproliferasi dihasilkan senyawa 1-5. Struktur kimia senyawa 1-5 diidentifikasikan berdasarkan metode spektroskopi meliputi UV-Vis, IR, 1H NMR, dan 13C NMR dan diidentifikasikan sebagai asam-3,4-dihidroksi benzoat (1), asam galat (2), apigenin (3), kaempferol (4), dan kuersetin (5). Senyawa 1-5 diuji aktivitas antiproliferasi terhadap sel kanker lidah SP-C1 dan menunjukkan nilai IC50 berturutturut  100,  100,  100, 0,72 dan 0,70 mg/mL. Analisis pertumbuhan sel menggunakan MTT assay dilakukan untuk menguji pengaruh metabolit sekunder dari daun Rasamala terhadap pertumbuhan sel SP-C1. Analisis Flowcytometri dilakukan untuk melihat hambatan siklus sel SP-C1 yang diberi perlakuan kaempferol (4) dan kuersetin (5). Pengujian apoptosis dilakukan melalui analisis aktivitas kaspase-8 dan kaspase-9.  Penekanan ekspresi protein c-myc dilakukan melalui analisis Western blotting. Analisis data menggunakan uji ANACOVA, Tukey HSD dengan tingkat kemaknaan ?=0,05. Kaempferol (4) dan kuersetin (5) menghambat siklus sel pada fase G0-G1 dengan cara menekan ekspresi c-myc dan meningkatkan apoptosis dengan cara meningkatkan aktivitas kaspase-8 dan kaspase-9. Kesimpulan dari penelitian ini adalah metabolit sekunder dari daun Rasamala, yaitu kaempferol (4) dan kuersetin (5) memiliki aktivitas sebagai antikanker lidah manusia SP-C1 melalui hambatan siklus sel, menekan ekspresi c-myc dan menginduksi apoptosis...Kata kunci: Altingia excelsa, siklus sel, sel kanker lidah SP-C1, apoptosis, kaempferol, kuersetin.AbstractTongue cancer is a common malignant in the oral cavity and it has characterized with the aggressive cell growth, poor prognosis and often treated of life. For that reason, effective treatment with minimum side effect, accurate and easy to be found are needed. Herbal medicine is one of the answer because its secondary matabolities are believed to have an anticancer activity. Anticancer activity of Hammamelidaceae family has been reported. In the present study, secondary matabolities of Rasamala leaves (Altingia excelsa Nornha) is one of plant belongs to Hammamelidaceae family, was incubated within the human oral tongue cancer cell (SP-C1). The aims of study were to identify the secondary matabolities of Rasamala leaves, to examine the secondary matabolities of Rasamala leaves toward SP-C1 cell growth inhibition through analysis of cell cycle arrest, suppression of proto-oncogen c-myc expression, and induction of apoptosis via increased proteolityc activity of caspase-8 and -9. True experimental laboratories design using SP-C1 oral tongue cancer cell line was used. The dried leaves of Rasamala was extracted with methanol at room temperature and evaporated at reduced pressure resulting concentrated methanol extract (254 g). The concentrated methanol extract was dissolved in water and successively partitioned with n-hexane and ethyl acetate. All of the extracts was tested on their antiproliferative activity against tongue cancer cells SP-C1 in vitro, and the ethyl acetate extracts showed strongest antiproliferative activity. The ethyl acetate extract was separated their secondary metabolites by various chromatographic techniques guided by antiproliferasi activity to yield compounds 1-5. The chemical structure of compounds 1-5 were identified by spectroscopic methods including UV-Vis, IR, 1H NMR and 13C NMR and identified as 3,4-dihydroxy benzoic acid (1), gallic acid (2), apigenin (3), kaempferol (4), and quercetin (5). Compounds 1-5 were tested on their antiproliferative activity against cancer cells tongue SP-C1 and showed IC50 values of > 100, > 100, > 100, 0.72 and 0.70 mg/mL, respectively. Cell growth analysis using MTT assay was performed to examine the effect of  secondary metabolities of Rasamala leaves toward SPC1 cell growth. Flowcytometry analysis was delivered to test the cell cycle arrest of SP-C1 cell treated by kaempferol (4) and quercetin (5). Apoptosis assay was confirmed by caspase-8 and -9. The suppression of c-myc protein was done by Western blotting analysis. Data analysis using ANACOVA assay, Tukey HSD with the level of significance ?=0.05. Kaempferol (4) and quercetine (5) were markedly induced the cell cycle arrest in G0-G1 phase through the suppression of c-myc expression and increased apoptosis via up-regulation of caspase-8 and -9. In conclusion, secondary metabolites of Rasamala leaves, kaempferol (4) and quercetin (5) have a strong anticancer activity in human oral tongue cancer SP-C1 through induction of cell cycle arrest, suppression of c-mcy protein expression and induction of apoptosis.Keywords: Altingia excelsa, cell cycle, human oral tongue cancer SP-C1, apoptosis, kaempferol, quercetin.
Docetaxel Hidrat Menghambat Proliferasi dan Metastasis Sel Kanker Oral SP-C1 melalui Induksi Protein Maspin Supriatno, Supriatno
Journal of Dentistry Indonesia Vol 15, No 1 (2008): April
Publisher : Faculty of Dentistry, University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.609 KB) | DOI: 10.14693/jdi.v15i1.77

Abstract

Human oral tongue cancer (SP-C1) is thought to be a high grade malignancy. Despite advances in surgery, radiotherapy, chemotherapy and combination therapy, prognosis and survival of patients with human tongue cancer have not significantly improved over the past several decades. Treatment options for recurrent or refractory tongue cancer are limited. Therefore, as a strategy for refractory cancer, anti-mitotic chemotherapy and its mechanisms are of considerable interest, including those using docetaxel hydrate for inducing maspin protein. In the current study, the mechanisms responsible for growth suppression and metastasis of SP-C1 by docetaxel hydrate through induction of maspin regulation were investigated. To evaluate in vitro cell proliferation and cell metastasis, MTT and out-growth assays were performed, respectively. Furthermore, the expression of maspin mediated by docetaxel hydrate was analysed by Western blotting. The results showed that treatment with 50 g/ml docetaxel hydrate significantly suppressed SP-C1 cell growth from day 1. Strong inhibition of metastasis of SP-C1 cells was also shown by treatment with 50 g/ml of docetaxel hydrate. Moreover, a significant induction of maspin regulation was detected in cells treated with 10 and 50 g/ml of docetaxel hydrate. However, the same protein level was demonstrated in -tubulin expression. These findings suggest that docetaxel hydrate may have potential for powerful anti-mitotic chemotherapy through induction of maspin regulation.DOI: 10.14693/jdi.v15i1.77
In vivo gene therapy with plasmid DNA-Tuberous sclerosis complex-2 (TSC-2) in an oral squamous cell carcinoma Supriatno, Supriatno
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 37, No 02 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.272 KB)

Abstract

Background: Tuberous sclerosis complex (TSC) is an inherited syndrome in which affected individuals are at increased risk for developing benign tumors. Mutation of the TSC-2 gene encoding tuberin on chromo-some 16p13.3 give rise to the clinical disorder of tuberous sclerosis characterized by the development of hamartomas.Objectives: In the present study, I conducted to examine whether overexpression of TSC-2 can affect the growth of human oral squamous cell carcinoma (OSCC) which have different expression level of p27KP. Methods: I constructed an expression vector containing sense-oriented rat TSC-2 cDNA with pcDNA3.1 (Invitrogen), and transfected to oral squamous cell carcinoma (B88) to regulate the expression of TSC-2 gene in each transfectant. Western blot analysis was carried out to determine the protein level of TSC-2. Therefore, Tumor volume and body weight of nude mice was examined using tumorigenicity assay.Results: Overexpression of TSC-2 exerted the growth inhibitory effect of oral squamous cell carcinoma. Sense-oriented B88-TSC-2 cancer cells, have high expression of p27KP1. Moreover, tumor induced by TSC2 up-regulated transfectans become much smaller than those of control cells.Conclusion: overexpression of TSC-2 may exert the antitumor effect on oral squamous cell carcinoma through p27KP induction. Key word: TSC-2 - transfection - oral squamous cell carcinoma - pcDNA3.1 - mutation. 
EVALUASI KINERJA GURU PROFESIONAL DALAM MENYUSUN PERENCANAAN DAN PELAKSANAAN PEMBELAJARAN Supriatno, Supriatno; Herpratiwi, Herpratiwi; Rosidin, Undang
Jurnal Teknologi Informasi Komunikasi Pendidikan Vol 3, No 1 (2015): Jurnal Teknologi Informasi Komunikasi Pendidikan
Publisher : Jurnal Teknologi Informasi Komunikasi Pendidikan

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Abstract

The purpose of this study was to obtain information about the teachers’ ability to plan learning and implementing the learning. The research population was a teacher of senior high school in Tanggamus who has been certified amounted to 342, while the sample of 40 people who were taken in proportion. The ability of the district high school profesional teacher Tanggamus in planning the learning process, is generally good enough(score 2.91), in particular the teachers’ ability to formulate learning objectives is good average (score of 2.92), the teachers’ ability in selecting and organizing teaching materials is good average (score 2.93), the teacher’ ability in selecting learning resources / learning media category is enough(score 2.89), the teachers’ ability to determine instructional methods are still good enough (score 2.91) and teachers’ ability to plann learning outcomes categories is still good enough(score2.86).Tujuan penelitian ini untuk memperoleh informasi tentang kemampuan guru dalam merencanakan pembelajaran dan melaksanakan pembelajaran. Populasi penelitian ini adalah guru SMA Kabupaten Tanggamus yang telah bersertifikasi berjumlah 342 orang sedangkan sampel berjumlah 40 orang yang diambil secara proporsional. Kemampuan guru profesional SMA Kabupaten Tanggamus dalam merencanakan proses pembelajaran, secara umum cukup baik (skor 2,91), secara khusus kemampuan guru dalam merumuskan tujuan pembelajaran rata-rata cukup baik (skor 2,92), kemampuan guru dalam memilih dan mengorganisasikan materi ajar rata-rata cukup baik (skor 2,93), kemampuan guru dalam memilih sumber belajar/media pembelajaran dalam kategori cukup (skor 2,89), kemampuan guru dalam menentukan metode pembelajaran masih cukup baik (skor 2,91 dan kemampuan guru merencanakan penilaian hasil belajar masih katagori cukup baik (skor 2.86).Kata Kunci: evaluasi, kinerja guru, pembelajaran
ANALISIS LOGAM BERAT PB DAN CD DALAM SAMPEL IKAN DAN KERANG SECARA SPEKTROFOTOMETRI SERAPAN ATOM Supriatno, Supriatno; Lelifajri, Lelifajri
Jurnal Rekayasa Kimia & Lingkungan Vol 7, No 1 (2009): Jurnal Rekayasa Kimia & Lingkungan
Publisher : Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

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Abstract

The analysis of heavy metals Pb and Cd concentrations in fish and oyster have been carried out by atomic absorption spectrophotometer (AAS) method. The wet digestion method was used for preparation sample prior to AAS detection. Sample was collected from three different location rivers at Lamnyong, Pantee Pirak and Lumbago. The result showed that the Pb and Cd concentration in fish and oyster at difference location were obtained not difference significantly. Pb and Cd contents in fish and oyster were found below lethal concentration. The AAS instrument was still valid to use as the instrument which is obtained of accuration of 0.65% and precise of 0,019 ppm still lower than threshold value of 1% and 0.04 ppm.Keywords: atomic absorbance spektrophootometry, fish, kerang, heavy metal
FLAVANOIDS FROMTHE STEMBARK OF CHISOCHETON PENTANDRUS(MELIACEAE) Supriatno, Supriatno; Hidayat, Ace Tatang; Farabi, Kindi; Abdullah, Fajar Fauzi; Nurlelasari, Nurlelasari; Herlina, Tati; Supratman, Unang; Awang, Khadijah
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 3, No. 2, November 2017
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Syarif Hidayatullah State Islamic Uni

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.628 KB) | DOI: 10.15408/jkv.v0i0.6077

Abstract

Two flavanoid compounds, catechin (1) and epicatechin (2), have been isolated from the stembark of Chisocheton pentandrus. The chemical structure of compounds1and2were identified byspectroscopic data including, UV, IR, NMR (1H, 13C, DEPT 135°, HMQC, HMBC, 1H-1H COSY) and MS and by comparing with previously reported spectral data. Compounds 1 and 2, were isolated in this plant for first time and showed no cytotoxic activity against MCF-7 breast cancer cells.
PENGARUH KONSENTRASI EKSTRAK ETANOL BONGGOL NANAS (Ananas comosus (L.) Merr) TERHADAP APOPTOSIS KARSINOMA SEL SKUAMOSA LIDAH MANUSIA Susanto, Hendri; Naritasari, Fimma; Supriatno, Supriatno
Majalah Obat Tradisional Vol 15, No 1 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (571.946 KB) | DOI: 10.14499/mot-TradMedJ15iss1pp%p

Abstract

Karsinoma  sel  skuamosa  lidah  merupakan  salah  satu  keganasan  yang sering terjadi di rongga mulut. Perawatan yang ada saat ini meliputi pembedahan, radioterapi, kemoterapi, maupun kombinasi ketiganya. Salah satu perawatan yang sedang  dikembangkan  antara  lain  adalah pencarian bahan alam/herbal yang dapat menginduksi  apoptosis  sel kanker.  Bromelain  memiliki  potensi  antikanker  salah  satunya  dengan menginduksi apoptosis. Bonggol nanas merupakan salah  satu bahan herbal  yang potensial  dikembangkan  untuk  perawatan  alternatif  karena  adanya  kandungan enzim  bromelain. Tujuan penelitian ini adalah  untuk mengetahui pengaruh konsentrasi ekstrak etanol bonggol nanas terhadap apoptosis biakan sel karsinoma skuamosa lidah manusia. Biakan  sel  karsinoma skuamosa  lidah manusia  diberi  perlakuan ekstrak  etanol  bonggol nanas tiga  konsentrasi  dibawah  nilai  IC50,  yang  diperoleh  dari  uji  sitotoksisitas,  yaitu konsentrasi 5.000, 5.500, dan 6.000 µg/ml. Pada uji apoptosis, setelah diinkubasi selama  24  jam,  sel  diwarnai  dengan  flurokrom  ethidium  bromide  dan  acridine orange. Pengamatan dan perhitungan dilakukan di bawah mikroskop flurescence. Sel  hidup  tercat  berwarna  hijau,  dan  sel  yang  mengalami  apoptosis  berwarna kuning  hingga oranye.  Analisis probit digunakan untuk menentukan nilai IC50 ekstrak etanol bonggol nanas dan Uji korelasi  pearson digunakan untuk mengetahui hubungan konsentrasi ekstrak etanol bonggol nanas dengan prosentase sel apoptosis. Hasil penelitian menunjukkan nilai IC50 esktrak etanol bonggol nanas pada biakan karsinoma sel skuamosa lidah. Penelitian ini juga menunjukkan terdapat hubungan positif antara konsentrasi ekstrak  etanol  bonggol  nanas  dan  apoptosis  (r  =  0,999,  p<0,05).  Kesimpulan  pada  penelitian  ini  adalah  bahwa  bonggol  nanas  mampu menginduksi  apoptosis  dan  terdapat  peningkatan  persentase  apoptosis  sel  yang sebanding dengan peningkatan konsentrasi ekstrak etanol bonggol nanas. 
Pengaruh Ritma Circadian Terhadap Produksi Volatile Sulfur Compounds (VSC) Oral Supriatno, Supriatno
Majalah Kedokteran Gigi Indonesia Vol 20, No 1 (2013)
Publisher : Majalah Kedokteran Gigi Indonesia

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Abstract

Volatile sulfur compounds (VSCs) oral dihasilkan dari produk putrifikasi mikroba gas hidrogen sulfida (H2S), metil merkaptan (CH3SH) dan dimetil sulfida [(CH3)2S] yang merupakan gas utama penyebab halitosis. Ritma circadian mempunyai pengaruh terhadap fungsi beberapa organ tubuh termasuk sekresi saliva, produksi hormon, fungsi sistem tubuh, dan aktivitas mikroorganisma. Penelitian bertujuan menguji pengaruh ritma circadian terhadap produksi VSC oral yang diukur menggunakan OralChroma portable. Penelitian dilakukan dengan mengukur gas VSC individu yang sama pada pagi, siang dan malam hari di laboratorium riset terpadu FKG UGM. Hasil pengukuran H2S, CH3SH dan (CH3)2S diuji menggunakan analisis statistik Anava dua jalur dilanjutkan uji LSD dan uji korelasi Pearson dengan derajat kemaknaan 95%. Hasil penelitian menunjukkan terdapat perbedaan yang sangat bermakna antara produksi gas H2S, CH3SH dan (CH3)2S dengan waktu pengukuran (efek circadian) (p=0,000). Perbedaan sangat bermakna diketahui pula pada pengukuran gas H2S dan (CH3)2S antara pagi, siang dan malam (p=0,01 dan p= 0,00), serta pengukuran gas CH3SH siang dan malam (p=0,006), tetapi tidak pada CH3SH pagi hari (p=0,061). Produksi gas H2S tertinggi diketahui pada pagi hari (mean 1,198 ng/10 ml, CH3SH pada malam hari (mean 0,099 ng/10 ml), dan (CH3)2S pada siang hari (mean 1,216 ng/10 ml). Kekuatan hubungan pengukuran antara ke tiga gas dengan efek circadian diketahui sebesar r=0,738. Disimpulkan bahwa ritma circadian berpengaruh terhadap produksi VSCs oral. Produksi gas H2S dan (CH3)2S berbeda antara pagi, siang dan malam hari, sedangkan produksi gas CH3SH berbeda hanya pengukuran siang dan malam hari. Produksi gas H2S tertinggi diketahui pada pagi hari, gas CH3SH pada malam hari, dan gas (CH3)2S pada siang hari. Maj Ked Gi. Juni 2013; 20(1): 14 - 20.ABSTRACT: The Effect Of Circadian Rhythm To Oral Volatile Sulfur Compounds Production. Oral volatile sulfur compound (VSC) is produced from microbial purification of hydrogen sulfide (H2S), methyl mercaptan (CH3SH) and dimethyl sulfide [(CH32S] gases. They are the main gases that cause halitosis. Circadian rhythm influenced the function of several organs of the human body including salivary secretion, hormone production, the body’s systems function, and activity of microorganisms.The purpose of this research is to examine the influence of circadian rhythm to oral VSC production measured by using a portable Oral Chroma. The research was carried-out by measuring the individual VSC gases in the morning, afternoon and evening at the integrated research laboratory, Faculty of Dentistry, UGM. Gases of H2S, CH3SH and (CH3)2S were tested by two-way ANOVA followed by Post-hoc LSD and Pearson correlation test with 95% significance level. The results showed the positive significant differences among the production of H2S, CH3SH and (CH3)2S with circadian time (p=0.000). Highly significant difference was also detected in amount of H2S and (CH3)2S gases in the morning, afternoon and evening (p=0.01 and p=0.00), as well as the amount of CH3SH gas in the afternoon and night (p=0.006), but not in amount of CH3SH gas in the morning (p=0.061). The highest production of H2S gas was known in the morning (mean 1.198 ng/10 ml), CH3SH gas was detected in the night (mean 0.099 ng/10 ml), and (CH3)2S gas was observed in the afternoon (mean 1.216 ng/10 ml). The strength of relationship among amount of three gases with circadian effects was r = 0.738. It is concluded that circadian rhythm markedly influences the production of oral VSCs. H2S and (CH3)2S gases production were significantly different among in the morning, afternoon and evening. However, amount of CH3SH gas production was significantly different only in the afternoon and the night. The highest gas production of H2S, CH3SH, and (CH3)2S was observed in the morning, in the night, and in the afternoon, respectively.