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KEANEKARAGAMAN DAN AKTIVITAS ENZIMATIS KAPANG RIZOSFER KACANG MEONGAN (Aeschynomene americana L.) DI DESA SUKOLILO BARAT, KECAMATAN LABANG, KABUPATEN BANGKALAN, MADURA Natassya, Griffin; Suprihadi, Agung; Rukmi, MG Isworo
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Molds are widely distributed in nature, they even present in extreme environment, such as hot and dry soil. Molds which can grow in extreme environment has been adapted to xeric environment by producing enzymes  with  special  characteristics.  The  aim  of  this  study  to  determine  the  diversity  of  molds  from A.americana L. rhizosphere at West Sukolilo Village, Labang district, Bangkalan Regency, Madura and also to examined their cellulolytic and proteolytic activities. Isolation of molds were done by using spread and dilution methods. Molds identification were done by macroscopic and microscopic examined. The molds diversity was calculated using Shannon-Wiener index. Semiquantitative enzyme examination were done using Carboxymethil Cellulose (CMC) agar for cellulolytic ang liquid gelatin 12% for proteolytic. Results of this study showed that mold diversity isolated from   A. americana L. rhizosphere was moderate (1,8-2,7) with total of 43 species, comes from 7 genus i.e. Aspergillus, Penicillium, Fusarium, Aureobasidium, Byssochlamys, Paecilomyces, and Trichoderma.  The  highest  index  of  cellulolytic  produced  by  Aspergillus  sydowii  (2,35),  while  highest proteolytic activity produced by Aspergillus flavus (86%). Keywords : molds, diversity index,xeric, cellulolytic, proteolytic
Biodegradasi Senyawa Hidrokarbon Oleh Strain Bacillus cereus(VIC) Pada Kondisi Salinitas Yang Berbeda Bhaktinagara, Reza Auliarahman; Suprihadi, Agung; Raharjo, Budi
Jurnal Akademika Biologi Vol. 4 No. 3 Juli 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacillus cereus has been noted as hydrocarbonoclastic microbe that has ability to degrade hydrocarbons in non-saline conditions and some often to be found on high salinity environtment conditions. The purpose of this research is to determine the ability of strains Bacillus cereus (VIC) were isolated from non-saline environment to degrade hydrocarbons in crude oil on the different salinity condition. Bacillus cereus (VIC) was inoculated on the medium that has contaminated by crude oil with salinity level of 0,3 , 9,4 , dan 19,6 and incubated for 15 days. Determination of microbial growth is by using Total Plate Count (TPC) method along with determination of Total Petroleum Hydrocarbon (TPH) percentage using gravimetry method for every 5 day. The determination of microbial growth showed that Bacillus cereus (VIC) has the tolerancefor salinity level up to 19,6 because it is able to grow to a density of 6.9 x 106 CFU/ml on the 15th day. Bacillus cereus (VIC) is also able to degrades hydrocarbons on crude oil pollutants that indicated from degradation of TPH percentage in the medium up to 21% during 15 days incubation period on the medium with salinity level of 19,6 . Biodegradation using Bacillus cereus (VIC) can increase TPH degradation on the medium up to 19,8% than TPH degradation because of weathering. Keywords: Biodegradation, Bacillus cereus(VIC), Total Petroleum Hydrocarbon (TPH), Salinity.
UJI AKTIVITAS KITIN DEASETILASE ISOLAT BAKTERI DARI KAWASAN GEOTERMAL DIENG Akhsani, Ghaida Afra; Suprihadi, Agung; pujiyanto, Sri
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Chitinolytic bacteria is a bacterium, which is able to degrade chitin. This ability is obtained from the resulted chitinolytic enzyme. Chitin deacetylase (EC 3.5.1.41) is one of the chitinolytic enzymes, which be able to convert chitin into its derivatives. For this reason, chitin deacetylase has a chance to be an environmentally enzymatic converter of chitin. In addition, chitin derivatives have a wider potential in many fields. The objectives of this study were to obtain bacterial isolates from the mud of Sikidang Crater in Dieng geothermal field that producing chitin deacetylase enzyme, and to determine its activity characteristic of (optimum time production, optimum pH, and effect of 1 mM divalent metal ions) from the resulted chitin deacetylase enzyme. This research used completely randomized design. The data were analyzed using One Way ANOVA and Tukey HSD test. The results showed that KSR HA 24 isolates were able to produce chitin deasetylase with optimum enzyme activity of 0.668 U / ml at 18 hours production time. Optimum activity of chitin deacetylase occurred at pH 5 of 0.75 U / ml. Chitin deacetylase activity with 1 mM addition of divalent metal ions produce activator metal ions, including Mg2+, which increased the activity up to 154.43%, Fe2+ the activity up to 144.63%, and Cu2+ the activity up to 110.41%. Inhibitor metal ions, including Zn2+, which decreased the activity to 93.77%, and Mn2+ the activity to 86.46%.Keywords: Chitinolytic, Chitin Deacetylase, Enzyme Activity, pH, Divalent Metal Ions
UJI AKTIVITAS ANTIBAKTERI EKSTRAK BIJI KELOR (MORINGA OLEIFERA L.) TERHADAP PERTUMBUHAN BAKTERI STAPHYLOCOCCUS AUREUS DAN BAKTERI ESCHERICHIA COLI Wigunarti, Anggia Hesti; Pujiyanto, Sri; Suprihadi, Agung
Berkala Bioteknologi Volume 2 Nomor 2 November 2019
Publisher : Berkala Bioteknologi

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Abstract

Moringa oleifera L. or moringa is one of the plants that can be used as a traditional herbal medicine because it has antimicrobial, anti-tumor, anti-pyretic, anti-oxidant and anti-inflammatory properties. The purpose of this study was to determine the antimicrobial activity of Moringa seed extract against Staphylococcus aureus and Escherichia coli. Extraction of active compounds from moringa seeds was carried out by maceration method. The maceration process with two solvents, polar solvent using 96% ethanol, and second with non-polar solvents using n-hexane. The macerate results are then evaporated in 60oC temperature over 1 hour . The test is done by diffusion to use a 6 mm paperdisk with two test of bacteria Staphylococcus aureus and Escherichia coli. The concentration of extract solution to be tested are 75%, 50% and 25%. The positive control was using 0,1 mg / mL chloramphenicol and negative control using DMSO. Experimental design using a completely randomized design and analyzed using the Analysis of Variance (ANOVA) and if the data obtained showed significantly different results at the 5% test level then continued with the Duncan test to determine differences between the treatments. From the results of testing the antimicrobial activity of Moringa seed extract in 96% polar ethanol solvent showed the presence of antibacterial activity against both of the test bacteria Staphylococcus aureus and Escherichia coli. The highest antibacterial activity occurs at a concentration of 75%. The diameter of the inhibitory zone of Staphylococcus aureus and Escherichia coli was 14,75 and 3,50. Non-polar n-hexane solvents showed no antibacterial activity in the two test bacteria Staphylococcus aureus and Escherichia coli. Key words:Moringa oleifera L., extracts, antibacterial, S.aureus, E.coli
MIX CULTURE INOCULANT PRODUCTION OF PHOSPHATE SOLUBILYZING AND INDOLE ACETIC ACID (IAA) PRODUCER RHIZOBACTERIA WITH AMBARAWA PEAT SOIL RAWAPENING AS CARRIER Raharjo, Budi; Suprihadi, Agung
JURNAL SAINS DAN MATEMATIKA Volume 18 Issue 2 Year 2010
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

ABSTRACT---Plant production development  is the main goals  that do for increase the farming quality to fulfill the man needed  in  food. One of the ways is intensive farming, by using organic or inorganic  fertilizer. Phosphate is the essential  for plants. IAA is the necessary plant regulator for the root. Both phosphate  and IAA need in  plant  growing  and production.  Biological  fertilizer  is  fertilizer  with  microbial  as the main material. Bacillus sp.DUCC-BR-K1.7, Bacillus sp. DUCC-BR-KI3, Bacillus sp.DUCC-BR-KI.2b  and Pseudomonas fluorescens, Azotobacter chroococcum, Azotobqcter vinelandii, Azospirillum sp. and Azotobacter brazilensis are bacteria that can solubilization  the phosphate  and IAA  synthesize.  Those bacteria can be used as  inoculants or biological  fertilizer  that put on carrier. One way to support  the aim is giving  the altemative carrier with  suitable composition. The carrier should be support bacterial life  during the storage.  The aim ofthis  research  is find  ths right  consortia so can be used to optimized  viabilities  of  culture Bacillus  sp. DUCC-BR-KIJ,  Bacillus sp. DUCC-BR-K1.3, Bacillus sp. DUCC-BR-KI.2b, P. flourescens, A. chroococcum, A. vinelandii, Azospirillum  sp., and  A. brazilensis in mix  culture  on peat soil  as carrier. This research done in Microbiology Laboratory of Biology Department Diponegoro University. Subculture and activated culture in Nutrient  Broth medium, make the growth curve to the biomass production, make the inoculums, prepare the peat soil, biomass production and mixed biomass with  the carrier, enumeration bacterial culture viability  test in carrier during the storage  by TPC method.  The  results  show  that  all  consortia  culture  bacteria viabiliry Bacillus  sp. DUCC-BR-KI.3  with Azospirillum (A) and Bacillus  sp. DUCC-BR-K1.7, with P.  flourescens (B)  still viable and increasing number of population during seven weeks storage with  l0 t-n CFU/g at To and up to  l0 r3-ro CFU/g at  the end storage. The  consortium  C  between Bacillus  sp.  DUCC-BR-KI.2  b  and A-  brssilensis  increasing  the  number  of population  and  still viable during eight weeks  storage  (1013  - 1014  CFU/g).Key words: biofertilizer, peat soil, viability,  mix cultare, consortiumPermalink : http://ejournal.undip.ac.id/index.php/sm/article/view/3138
PRODUKSI INULINASE OLEH KHAMIR Pichia manshurica DUCC Y-015 PADA TEPUNG UMBI DAHLIA (Dahlia variabilis Willd.) DENGAN VARIASI KONSENTRASI MnSO4.H2O DAN WAKTU INKUBASI Abadianti, Berlian; Suprihadi, Agung; wijanarka, w
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Inulinase (E.C. 3.2.1.7) is an enzyme which able to hydrolyze an inulin into fructose and fructooligosaccharides.  The main application of inulinase enzyme in the food industries is as the ingredient in producing High Fructose Syrup (HFS). Moreover, the other important applications of inulinase enzyme are to produce ethanol, inulooligosacarida (IOS), fruktooligosacarida (FOS), pullulan, sorbitol, etc.  Pichia manshurica DUCC Y-015 is kind of yeast that is capable in producing inulinase in medium containing inulin. Optimization of inulinase enzyme production needs to be done to increase inulinase production, the way that could be conducted is by the addition of metal ion and optimization of incubation time. The purpose of this research is to investigate the effect of adding mangan ion (MnSO4.­H2O) and incubation time. In conducting this study, the researcher applies experimentally research by using Randomized Complete Block Design (RCBD) factorial pattern. The first factor is concentration of MnSO4.H2O, with concentration level 0 mM (M0), 0,1 mM (M1), 0,5 mM (M2). The second factor is the variation of incubation time, i.e. 6 hours (I6), 12 hours (I12), and 18 hours (I18) with three times repetition. The collected data were analyzed using ANOVA 5% signification (α= 0,05) and completed by the Duncan test. The result of analysis shows that variation of MnSO4.H2O concentration and incubation time does not significantly influential on inulinase activity of Pichia manshurica DUCC Y-015.Keywords: Inulinase, Inulin, Pichia manshurica DUCC Y-015, MnSO4.H2O, Incubation time.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK TUMBUHAN Euphorbia hirta L. TERHADAP Ralstonia solanacearum, Escherichia coli, DAN Staphylococcus aureus SECARA IN VITRO Angelika, Genoveva Preta; Suprihadi, Agung; pujiyanto, Sri
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Biocontrol using Patikan kebo (Euphorbia hirta L.) plant is an alternative solution to control pathogenic bacteria. Such wild plant is known to contain active compounds with antibacterial activity such as tannins, alkaloids, flavonoids, saponins, and phenols. This study aims to determine the antibacterial activity of the methanol extract of E. hirta against R. solanacearum, E. coli and S. aureus. The extraction method of E. hirta was maceration with methanol solvent, while antibacterial activity test using the agar diffusion method (Kirby-Bauer) with test bacteria was R. solanacearum, E. coli and S. aureus. E. hirta extract tested was pure extract (100%). Observed response was diameter of inhibitory zone formed around the paper discs that had been smeared with E. hirta extract on the media. Analysis of the data using a Completely Randomized Design (CRD) 1 factor (test bacteria) with three times repetition, followed by a further test of Duncan with 95% confidence level. The results indicated that E. hirta produced extraction yield of 6,45%. Antibacterial activity of E. hirta extract against R. solanacearum, E. coli and S. aureus was indicated by Inhibitory Zone Diameter (HZD), respectively for 21,8 mm, 18,26 mm and 17,06 mm. The results of this study showed that the methanol extract of E. hirta plant had antibacterial activity against R. solanacearum, E. coli and S. aureus, thus can be used as a biocontrol agent of bacterial wilt disease in plants caused by R. solanacearum and human disease caused by E. coli and S. aureus. Keywords: Euphorbia hirta, Ralstonia solanacearum, Escherichia coli, Staphylococcus aureus, antibacterial activity, diffusion method
EKSPLORASI RHIZOBAKTERI INDIGENOUS TANAMAN CABAI RAWIT (Capsicum frustescens Linn.) DARI PERTANIAN SEMI ORGANIK DESA BATUR KABUPATEN SEMARANG SEBAGAI AGEN HAYATI PENGENDALI PERTUMBUHAN JAMUR Fusarium oxysporum f.sp capsici Prastya, M Eka; Suprihadi, Agung; Kusdiyantini, Endang
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rhizobacteria is a group of bacteria that live in the around of plant roots. This type of bacteria known have the ability to stimulate plant growth by producing growth hormone, as well as to inhibit the growth of plant pathogens synthesize compounds with antibiotics or extracellular enzymes. The purpose of this study was to obtain and describe the morphological, biochemical and genetic isolates rhizobacteria of semi-organic farmland Semarang District village Batur which has the ability as a biological control agent of fungal pathogens Fusarium oxysporum f.sp capsici. The results obtained fifteen isolates the majority rhizobacteria bacilus shaped gram-positive and classified. Rhizobacteria inhibition test capabilities against pathogenic fungi was performed using dual culture test and test biomass. Dual culture test results showed that the inhibition of isolates E1 has a 3.77%, 1.88% isolates E3 and E15 isolates 22%. The biomass tests show E15 isolates capable of inhibiting the growth of pathogenic fungi best with the smallest weight of fungal biomass 0.0386 grams. The results of the molecular characterization based on 16S rRNA gene sequences to known that E15 isolates has similar with Bacillus cereus strain ATCC 14579 with similarity of 97%. The results of the biochemical characterization of isolates E15 has similarities with B. cereus species that is catalase positive, motile, have endospores, is able to hydrolyze starch and ferment glucose.Keywords: rhizobacteria, Fusarium oxysporum f.sp capsici, gen 16S rRNA, Bacillus cereus
ISOLASI DAN KARAKTERISASI BAKTERI PENDEGRADASI PESTISIDA DICOFOL DARI TANAH SAWAH DI KABUPATEN KARAWANG Pratiwi, Anindita Ayu; Suprihadi, Agung; Raharjo, Budi; Wahyudi, Priyo; Parmiyatmi, Sih
Jurnal Akademika Biologi Vol. 1 No. 1 Oktober 2012
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Karawang was the center of rice production, but rice production in paddy fields in Karawawang often faces obstacle hopper pest that caused crop failure. These condition prompted the farmers to using dicofol pesticides excessively, thus caused pollution of pesticides. The purpose of this research were get the isolates bacteria that could degrade the pesticide dicofol in Karawang paddy fields and discovered characteristics of bacteria that have potential to degrade pesticides. The research was conducted with the isolation and characterisation of bacteria from Karawang paddy soil, the characterisation of isolates bacteria by observation morphological and phenotypic include staining Gram and biochemical tests, test isolates bacteria resistence of dicofol, measuring the growth curve and High Performance Liquid Chromatography (HPLC) for discovering of decreased dicofol concentration during 24, 48, and 72 hours of incubation time. The result is six isolates, that are AA1, AA2, AA3, AA4, AA5, and AA6. Isolates AA1 is suspected as genus Phenylobacterium, isolates AA3 and AA6 are suspected as genus Pseudomonas, AA4 isolates is suspected as genus Bacillus, isolates AA5 is suspected as genus Alcaligenes, but isolates AA2 not yet be identified. The results indicate that isolate AA6 has most resistent to dicofol and most number of cells bacteria, that is 7.9x108. Isolate AA6 is also the most effective in degrading dicofol as much as it can reduce the concentration in amount of 76.0 ppm (84.45%) in MSM medium + 90 ppm dicofol. Keywords : Isolation, Characterisation, Bacteria, Resistention, Degradation, Dicofol Pesticide
ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI “TEMPOYAK” Aisyah, Arina; Kusdiyantini, Endang; Suprihadi, Agung
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Tempoyak is a food made from fermented durian flesh with the addition of salt and brooded for seven days. Tempoyak quality is strongly influenced by the presence of microbes involved during the process of fermentation. This study aimed to isolate and characterize lactic acid bacteria (LAB) that play a role in the tempoyak fermentation process, and to perform proximate analysis of the durian and tempoyak. Isolation was done on 0, 3rd, 5th, and 7th day fermentation with streak methods. The isolates then underwent morphology and motility observation, biochemical tests, and proximate analysis.  The isolation of tempoyak resulted seven isolates of bacteria that had different colony and cell morphology. Gram staining of the bacterial cell produced a purple color with the rod and spherical shape. Motility test resulted non motile bacteria. Catalase test of bacteria isolates produced negative catalase. Isolates showed positive results in test for acid production and carbohydrate fermentation. pH decrease from 6.88 to 5.74 on the last day of brooding. Seven isolates obtained had characteristics that were similar with the characteristic of LAB, which was Gram positive, rod shaped or spherical, non motile, negative catalase, and produce acid. Tempoyak nutrient contained, ie 15.12% moisture content, 27.03% ash content, 2.69% fat content, 6.37% protein content, and 48.79% carbohydrate content. Keywords: tempoyak, LAB, morphology, biochemistry, proximate