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PRODUKSI SIRUP GLUKOSE DARI PATI SECARA ENZYMATIK Supriyo, Edy; Kurniawan, Dedy
METANA Vol 6, No 01 (2009): April 2009
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4062.506 KB) | DOI: 10.14710/metana.v6i01.1800

Abstract

  Abstract   In the prodacution of tapioca starch, before the starch being dried, glucose syrup could be produced wih ghigh concentrotion of maltose and glucose using enzymatic processes. Hidrolized tapioca starch using a-amylase enzyme which comes from Aspergilus niger produce concentration of syrup i.e. hanya 42,88 %, while using commercial a-amylase enzymes of NOVO (AMG 3001) the conversuion yiled was 114%.  Beside the ash content is still high make the syrup colour become cloudy, whilst glucose syrup produced with a-amylase enzymee is very clear.   Keywords : syrup glucosa, Aspergilus niger, a-amylase, tapioca starch
BIOKATALIS LIPASE RHIZOPUS ORYZAE PADA REAKSI TRANSESTERIFIKASI LIPID TERSTRUKTUR KAYA ASAM LEMAK OMEGA -3 Wahyuningsih, Wahyuningsih; Supriyo, Edy; Broto, R. TD. Wisnu
METANA Vol 11, No 02 (2015): Desember 2015
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (616.134 KB) | DOI: 10.14710/metana.v11i02.14752

Abstract

Abstrak                Immobilisasi merupakan teknik recovery enzim yang menjadi perhatian dalam beberapa tahun belakangan, dilakukan dengan bantuan support sebagai media yang dapat mencegah terlarutnya enzim. Metode immobilisasi yang digunakan dalam penelitian ini adalah metode adsorpsi, dengan menggunakan lipase rhizopus oryzae, reaksi yang diamati adalah reaksi transesterifukasi Lipid Terstruktur kaya omega-3. Tujuan Penelitian ini adalah, mengkaji teknik  immobilisasi enzim lipase dengan bantuan support dengan melakukan pengamatan  teknik  adsorpsi fisik  dan mengembangkan penggunaan enzim  lipase Rhizopus oryzae immobile sebagai  biokatalisator untuk reaksi transesterifikasi minyak ikan tuna. Optimalisasi kondisi operasi proses terhadap produktifitas lipid terstruktur kaya asam lemak omega-3, dengan kajian kecepatan reaksi. Tujuan utama dilakukan immobilisasi enzim adalah untuk melihat apakah enzim tersebut dapat digunakan kembali dalam reaksi selanjutnya (reuse). Hasil penelitian menunjukkan kondisi optimum reaksi  pada suhu 50oC, pH lipase 8 perbandingan ratio substrat (Minyak ikan tuna : asam laurat )1:10 selama 6 jam. Profil gliserida dari hasil asidolisis enzimatis adalah  78,1 % trigliserida, 32,2 % Digliserida dan 11,9% Monogliserida. Inkoorporasi asam laurat mencapai 62,8 mol %. Pada waktu inkubasi 12 jam, trigliserida menurun seiring dengan meningkatnya waktu inkubasi, sedangkan digliserida meningkat seiring dengan meningkatnya waktu inkubasi. Pada suhu reaksi di atas 50oC, trigliserida menurun seiring dengan meningkatnya suhu reaksi. Metode interesterifikasi ini cukup efektif untuk mensintesis lipid terstruktur spesifik.Kata kunci: Lipid terstruktur, immobilisasi lipase , minyak ikan tuna                                                                                                                                                                             Abstract                 Immobilization of an enzyme recovery techniques are a concern in recent years, carried out with the help of support as a medium that can prevent the dissolution of enzim. Method immobilization used in this study is the adsorption method, using rhizopus oryzae lipase, a reaction observed was a reaction transesterifikasi. Structured Lipids rich in omega-3 the aim of this study is, reviewing techniques immobilization of lipase with the assistance of support by observing techniques physical adsorption and develop the use of lipase Rhizopus oryzae immobile as biokatalisator for the transesterification reaction tuna fish oil optimization of operating conditions of the process to the productivity of structured lipids rich in fatty acids omega-3, with an assessment of the speed of the main reaction. Purpose  do immobilisation of enzymes is to see whether the enzyme can be reused in a subsequent reaction (reuse) the results of the research showed the optimum reaction conditions at 50 ° C, pH 8 comparisons ratio lipase substrate (tuna fish oil : lauric acid) 1:10 for 6 hours. Profil gliseride result is 78.1% asidolisis enzymatic triglycerides, 32.2% and 11.9% diglycerides Monogliserida. Inkoorporasi lauric acid reached 62.8 mol%. %. At the 12 hour incubation period, triglyceride decreases with increasing time of incubation, whereas diglycerides increased with increasing incubation time. At reaction temperatures above 50° C, triglycerides decreased with increasing reaction temperature. Interesterification method is quite effective to synthesize specific structured lipids.Keywords: structured lipids, immobilization of lipases, tuna fish oil 
OPTIMASI PRODUKSI PROTEIN SEL TUNGGAL DARI BAGASE TERHIDROLISA DENGAN FERMENTASI OLEH SACCAROMYCES CEREVICEAE Pudjihastuti, Isti; S, Margaretha T; Wahyuningsih, Wahyuningsih; Supriyo, Edy
METANA Vol 8, No 02 (2012): Desember 2012
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.631 KB) | DOI: 10.14710/metana.v8i02.6810

Abstract

The Came pulp (bagase) were main contained cellulose, through process delignification and hidrolized can be used to growting sacaromyces cereviceae yeast as fermented media, so can produced biomass microbial as known as single cell protein (SCP).Design experiment was random block with treatment nutrient added as long as fermented process. The measured parameter were protein contained in microbial biomass with Kehjdahl method. Optimum result were formed to 8 days fermentation and nutrient added (NH4)2SO4:1 gr,MgSO47H2O : 0,5 gr, molase (tetes) 20% : 2 ml to media 50 gr. Key word: bagase, Saccaromyces Cereviceae,SCP
BIOKATALISATOR LIPASE DEDAK PADI UNTUK PROSES ASIDOLISIS MINYAK TUNA DAN ASAM LAURAT Wahyuningsih, Wahyuningsih; Supriyo, Edy; Broto, R.T.D. Wisnu
METANA Vol 14, No 1 (2018): Juni 2018
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (876.074 KB) | DOI: 10.14710/metana.v14i1.18658

Abstract

Lipid terstruktur dengan medium chain fatty acid (MCFA) pada posisi luar dan polyunsaturated fatty acid (PUFA) pada posisi sn-2 memiliki nilai gizi dan absorbsi yang sangat baik. Dalam penelitian ini lipid terstruktur disintesis secara langsung melalui asidolisis enzimatis antara minyak ikan dan asam laurat. Reaksi dikatalisis oleh lipase dedak padi. Tujuan penelitian ini adalah mempelajari  perilaku dari reaksi asidolisis enzimatik minyak ikan tuna dan asam laurat, dengan kajian pengaruh biokatalis lipase dedak padi terhadap hasil asidolisis. Target yang ingin dicapai berupa data-data teknis laboratorium untuk perancangan, scale-up dan pengoperasian proses yang meliputi kinetika reaksi, studi produktifitas asam lemak, kondisi operasi yang optimum dan analisa tekno-ekonomi. Hasil penelitian menunjukkan bahwa konsentrasi lipase dan suhu reaksi optimum berturut-turut 10% dan 50oC. Rasio mol optimum minyak ikan dan asam laurat adalah 1:10, dihasilkan inkorporasi asam laurat mencapai 62,8 mol%. Pada waktu inkubasi 12 jam, trigliserida menurun seiring dengan meningkatnya waktu inkubasi, sedangkan digliserida meningkat seiring dengan meningkatnya waktu inkubasi. Pada suhu reaksi di atas 50oC, trigliserida menurun seiring dengan meningkatnya suhu reaksi. Metode interesterifikasi ini cukup efektif untuk mensintesis lipid terstruktur spesifik. Lipase dapat digunakan dengan baik untuk sintesa Lipid Terstruktur dari minyak ikan tuna dengan asam laurat. Kondisi optimum reaksi adalah pada suhu 50oC, konsentrasi lipase 10%, perbandingan ratio substrat (Minyak ikan tuna : asam laurat) 1:10 selama 12 jam. Profil gliserida dari hasil asidolisis enzimatis adalah  78,1 % trigliserida, 32,2 % digliserida dan 11,9% monogliserida Lipase Rice Bran Biocatalystator For Asidolysis Process Tuna Oil And Lauric Acid Lipid structured with medium chain fatty acids (MCFA) in the outer position and polyunsaturated fatty acid (PUFA) in sn-2 position has excellent nutritional value and absorption. In this study structured lipids were synthesized directly through enzymatic acididisation between fish oil and lauric acid. The reaction was catalyzed by a specific lipase of 1.3 from the tertiary carotid rugose. The aim of this study was to study the behavior of enzymatic acidic reactions of tuna and lauric acid oils, with the study of the effect of rice bran biocatalyst on acidic acid yield. The targets to be achieved are technical laboratory data for design, scale-up and operation of processes including reaction kinetics, fatty acid productivity studies, optimum operating conditions and techno-economic analysis. The results showed that the optimum lipase concentrate  and temperature of the reaction were 10% and 50oC, respectively. The mole ratio of fish oil and lauric acid was 1:10 in which the incorporation of lauric acid was 62,80% (mol). Incubation time, 12 h, triglyceride decreased with an increase in incubation time. In contrast, the diglyceride increased with an increase in incubation time. At temperature higher than 50oC, triglyceride decreased with an increase in reaction temperature. The methode of interesterification was proven to be effective in synthezed specific structured lipids. Lipase rice brand, can be used successfully for the synthesis of structured lipids from tuna oil with lauric acid. Optimum reaction temperature is 50oC, lipase concentration of 10%, the ratio of substrate ratio (tuna fish oil: lauric acid) 1:10 for time incubation 12 hours. Profile gliseride from results acidolysis enzymatic triglycerides were 78.1%, 32.2% 11.9% diglycerides and monoglycerides.
PRODUKSI FORMULASI INSEKTISIDA CAIR BENTUK EMULSIFIER CONCENTRATE YANG EFEKTIF GUNA MEMBASMI NYAMUK AEDES AEGYPTI DALAM USAHA MENCEGAH PENYAKIT DEMAM BERDARAH Supriyo, Edy; Abidin, Zainal; Nugraheni, Nugraheni; Wahyuni, Sri
METANA Edisi Khusus Februari 2011
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6779.819 KB) | DOI: 10.14710/metana.v0i0.1662

Abstract

Abstract   Liquid pesticide formulation in the form of emulsifier concentrate (EC) is more preferable because it does not form clogging and blocking in the tools and precipitating that harden. Based on fired capacity in the whole pesticide formulation industry, about 25 % produce emulsifier concentrate. In the emulsion formulation, the important point is unite insoluble matter become a homogeny and stable formulation for long period. The selection of emulsifier type is very important because it is influenced by quality and EC formulation stability. Result formula very good is 20 % propoxure; 5 % Malathion; 5 %; agrisol ; 20 % DBS; 45 % Xyline dan 5 % additive (%w/w), concentration affection test 0.025% v/v for thinner solvent in 20 strain aedes aegypti mosquitoes at Semarang killed on 2 hours = 4 mosquitoes, 4 hours = l0 mosquitoes, 6 hours = 16 mosquitoes and on 8 hours all mosquitoes had been died. From emulsion stability test, in oil phase and water phase was found terpentine as stable solvent in the emulsion form, with separated oil percentage at concentration 3mg/l about 42%. In water phase emulsion forms micro emulsion with white fog colors in whole concentration 1, 2 and 3 ppm   Keyword: Emulsifier Concentrate
TEKNOLOGI PRODUKSI PUPUK ORGANIK CAIR DARI LIMBAH SAMPAH RUMAH TANGGA DI KELURAHAN LEMPONGSARI, KODYA SEMARANG DENGAN KOMPOSER EM-4 Wahyuningsih, Wahyuningsih; Supriyo, Edy
METANA Vol 9, No 01 (2013): Juli 2013
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.528 KB) | DOI: 10.14710/metana.v9i01.7205

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Abstract In Semarang limited landfill waste,new problems.If problems not handled and managed properly,an increase of waste that occur each year could shorten justify the air.In addition,the waste can the quality of natural resources,causing floods,and cause some diseased. Research to be conducted has several specific objectives,as follomposer EM-4.Minimizing the volume of waste in Semarang environment develop a network tool composter for organic fertilizer production of large-scale environmental and examine the addition of decomposers EM-4 the formation of humus to improve the productivity of organic fertilizer in environmentally friendly, operating condition optimization weathering process household waste using waste decomposer EM-4. Composter network of productivity environmentally friendly is expected to replace the function of inorganic fertilizers Research results:the best is on addition EM-4 8 ppm,with the following results:value ratio C/N 14,77;P2O5 1,24%;K2O 0,42%  SNI: ratio C/N 10-20 ;0,1% min P2O5 ;0,2%min K2O Keyword: EM-4 composer,liquid compost organic,organic waste
PEMBERDAYAAN INDUSTRI KECIL KERIPIK PISANG DALAM UPAYA MENUJU INDUSTRI MANDIRI Pudjihastuti, Isti; Supriyo, Edy; Hartati, Retno
METANA Edisi Khusus Februari 2011
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3720.685 KB) | DOI: 10.14710/metana.v0i0.1648

Abstract

Abstract                                                                          The development of micro industries, in banana chip micro industry to the member of group which is culinary enterprise Pemalang speciallized is give suitable solution to consument for giving natural culinary with high nutrition value independently and cheap.The development of micro industries is circular activity that consist of briefing about the use of gelatse system in banana chip production by naturally and independently, the suitable tachnique of banana chip production, production of furnace completely by steack as pilot plan, assitancy when the producer makes snack in gelatse system. Moreover, the taste of snack more delicious, the market bigger.
SISTEM PENGOLAHAN LIMBAH GAS CIANIDA PADA INDUSTRI PESTISIDA STUDI KASUS PT. ALFA ABADI PESTISIDA - JAWA BARAT Supriyo, Edy
METANA Vol 5, No 2 (2007): Desember 2007
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4235.993 KB) | DOI: 10.14710/metana.v5i2.1906

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Abstract   Pesticide industries produce substance to kill pest and weeds, therefore product,side product, raw  material and its wastes are hazardous and poison. The main pollution from pesticides industries are air pollutant. According to property of the wastes, treatment wastes include combination of absorbtion, catalytic and condensation order to match with limiting value by government. After being treated, the component moritored i.e. CN was reduced from 10 mg/lt become 0.01 mg/lt   Keywords : cyanide, pesticide industry.  
EFIKASI FORMULA INSEKTISIDA BERBAHAN AKTIF PROPOXURE DAN MALATHION TERHADAP VEKTOR PENYAKIT DBD Supriyo, Edy; Broto, R.T.D. Wisnu; Pujihastuti, Isti
METANA Vol 14, No 1 (2018): Juni 2018
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (892.068 KB) | DOI: 10.14710/metana.v14i1.19163

Abstract

Beberapa formula insektisida tidak efektif untuk membasmi vektor penyakit Demam berdarah dengue sehingga digunakan formula kombinasi beberapa insektisida golongan organofosfat dan karbamat yang terbukti memenuhi standar Komisi Pestisida Indonesia. Penelitian ini bertujuan untuk  menguji efikasi formulasi campuran golongan organofosfat dan karbamat terhadap vektor DBD Aedes aegypti strain Semarang. Formulasi insektisida yang digunakan mempunyai komposisi propoxure 17 %, Malathion 3 %, agrisol 13 %, DBS 7 %, Xyline 55 %, dan additive 5 % (% berat). Uji efikasi (uji LD 50)dilakukan dengan menggunakan hewan uji nyamuk Aedes aegypti strain kota Semarang. Sebelum digunakan uji efikasi, formula insektisida cair diencerkan  10 kali dengan pelarut adthiner. Uji efikasi menunjukkan bahwa semua hewan uji tersebut 90% mati pada dosis 0,250 cc/L sehingga disimpulkan bahwa dosis yang terbaik untuk digunakan pada foging adalah 0,250  cc/ L.  Effication Test Of Insecticide Formula With Active Ingredient Of Propoxure And Malathion To DBD Disease Vector Several insecticide formulae have not been effective to eridicate the vector of dengue fever disease therefore the combination some insecticide of organophosphate and carbamate has been proved to match with Indonesian Pesticide Commision. The present work was aimed to do the effication test of  insecticide formulation of organophosphate and carbamate on vector of DBD. Insecticide formulation were consisted of 17 % propoxure, 3 % Malathion, 13 % agrisol, 7 % DBS, 55 % Xyline, and 5 % additive (% weight). Effication test (LD 50)use mosquitoes of Aedes aegypti Semarang strain. Prior the test the insecticide formulation was diluted 10 times with adthiner solvent. Effication test showed that 90%  mosquitoes was killed at dose of formulation 0,250 cc/L. Therefore it is conclused that 0,250 cc/L of insecticide formulation was recommed for Aedes aegypti fogging.
EFEKTIFITAS PEMAKAIAN SOLVENT SEBAGAI PELARUT FORMULA INSEKTISIDA DALAM MEMBASMI NYAMUK AEDES AEGYPTI GUNA MENCEGAH PENYAKIT DEMAM BERDARAH DENGUE Supriyo, Edy; Nugraheni, Fransisca Sri; Broto, R.T.D. Wisnu
METANA Vol 14, No 2 (2018): Desember 2018
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.11 KB) | DOI: 10.14710/metana.v14i2.21389

Abstract

Formula yang ada dipasar bebas hanya dapat membunuh nyamuk saja, maka dalam penelitian ini akan dilakukan  Produksi formulasi insektisida sebagai insektisida alternative pembunuh nyamuk dan jentik, dimana agrisol dan dodecyl benzen sulfonat merupakan emulsifier nonionic dan anionic sert bersifat hydrophilic. Penelitian ini bertujuan untuk menentukan konsentrasi emusifier yang tepat dalam proses formulasi insektisida, sehingga kestabilan emulsi antara minyak dengan air dalam waktu yang cukup lama Pada percobaan pembuatan emulsifier konsentrat kemudian dilakukan uji kestabilan emulsi antara minyak dan air serta uji effikasi Maka dapat diproduksi formula yang digunakan sebagai bahan dasar insektisida dalam membasmi nyamuk dan jentik nyamuk Aedes aigepty. Disamping itu juga dapat menjadi formula obat nyamuk (insektisida) alternatif yang mudah diaplikasikan oleh masyarakat. Hasil penelitian menunjukan bahwa emulsifier concentrat yang terbaik adalah pada percobaan dengan formula komposisi: 17,38% propoxure; Malathion 17,39% agrisol; 13,01%  DBS, 47,83 % Xyline dan 4,35% additive dalam % berat. Dengan total emulsifier yang digunakan 30,5 % w/w  equivalent dengan 30 EC  Dari uji kestabilan antara minyak dan air didapat pelarut yang terbaik adalah thiner, dengan membentuk micro emulsi pada lapisan air sampai 12 minggu lebih, kestabilan emulsi tercapai pada CMC 0,42 % v/v pada lapisan minyak. Dari uji effikasi dengan pengencer thiner sebanyak 10x setelah dianalisa profitabilitas sesuai LD 50, didapat dosis . 0,025 -0,25 % (v/v), baik untuk telur nyamuk maupun jentik Aedes aegypti dengan prosentasi kemation 45-90%  Effectiveness of Solvent Usage as a Solvent for Insecticide Formula in Eradicating Aedes Aegypti Mosquitoes to Prevent Dengue Hemorrhagic Fever Pesticide formulation which are available in market are only killed the mosquitos but not the larvae. Therefore Production formulation of insecticide as inscticide alternative to eridicate mosquitos and its larvae with agrisol and dodecyl benzen sulfonat as emulsifier nonionic and anionic hydrophilic. The reseach work was aimed to determine the best emulcifier concentrate in pesticide formulation  so that the emulsion of oil and water will be stable for a long time.  In reseach work the emulsifier concentrate was made, than emulsion stability test between oil and water as well as its effectivity were condected. This formulation could be used as active engridient for insecticide to kill Aedes aigepty mosquitos and its larvae and could be applied in the community.  The research showed that formulation consist of komposisi: 17,38% propoxure; Malathion 17,39% agrisol; 13,01%  DBS, 47,83 % Xyline dan 4,35% additive dalam % berat. Dengan total emulsifier yang digunakan 30,5 % w/w  equivalent 30% w/w.  Stability test of oil and water showed that the best solvent is thiner,  and formed micro emulsi on water film for more than 12 week.  Emulsion was stable by CMC 0,42 % v/v on oil film. From effication test and than analysis profitability revealed that LD 50, was . 0,025 -0,25 % (v/v), both for larvae and mosquitos presented dead 45-90%.