Articles

PROFILE OF 17ß-ESTRADIOL, VITELLOGENIN, AND EGG DIAMETER DURING GONAD MATURATION PROCESS OF STRIPED CATFISH PANGASIANODON HYPOPHTHALMUS Pamungkas, Wahyu; Jusadi, Dedi; Junior, Muhammad Zairin; Setiawati, Mia; Supriyono, Eddy; Imron, Imron
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4039.234 KB) | DOI: 10.19027/jai.18.2.152-161

Abstract

ABSTRACT This study was conducted to evaluate the profile of 17ß-estradiol (E2) and vitellogenin (Vtg) in plasma and egg diameter during gonad maturity process of striped catfish Pangasianodon hypophthalmus. Blood samples were collected from immature striped catfish, male and female with different stage of gonad maturity (stage I, II, III, and IV) to measure the concentrations of E2 and Vtg. Gonad maturity development of striped catfish was observed based on egg diameter. Result showed that E2 concentrations were the highest (843.65 pg/mL) on female with maturity stage III, the lowest on the male (26.34 pg/mL), and immature female fish (29.37 pg/mL). The protein band of Vtg was obtained on the plasma of the mature female (stage I, II, III and IV) with a molecular weight (MW) between 140?180 kDa, but it was not obtained on immature female dan male striped catfish. The highest concentration of Vtg was found in the plasma of the female fish with maturity stage III (87.34 mg/mL), then on the stage II (74.83 mg/mL), I (68.58 mg/mL), and IV (33.45 mg/mL). It showed that egg yolk formation occurred in the female mature. The average egg diameter was 0.107 ± 0.052 mm, 0.318 ± 0.086 mm, 0.864 ± 0.099 mm, and 1.041 ± 0.058 mm on the maturity stage I, II, III, and IV respectively. The increase of egg diameter along with development of gonad maturity stage indicated that egg development occurred due to the process of vitellogenesis and the addition of egg yolk on oocyte. Keywords : egg diameter, gonad maturity, striped catfish , 17ß?estradiol, vitellogenin ABSTRAK Penelitian dilakukan untuk mengevaluasi profil estradiol-17? (E2), vitelogenin (Vtg) dalam plasma dan diameter telur pada proses pematangan gonad ikan patin siam (Pangasianodon hypophthalmus). Sampel darah untuk pengukuran konsentrasi E2 dan Vtg plasma diperoleh dari ikan patin siam betina yang belum matang gonad, ikan jantan, ikan betina dengan tahap kematangan gonad yang berbeda (tahap I, II, III dan IV). Perkembangan kematangan gonad ikan patin siam diamati berdasarkan diameter telur. Hasil penelitian menunjukkan bahwa konsentrasi E2 tertinggi (843,65 pg/mL) pada ikan betina dengan kematangan tahap III, terendah pada ikan jantan (26,34 pg/mL), dan ikan betina tidak matang gonad (29,37 pg/mL). Pita protein Vtg pada sampel plasma diperoleh dari betina matang gonad (tahap I, II, III dan IV) dengan berat molekul antara 140-180 kDa, tetapi tidak diperoleh pada ikan patin siam betina yang belum dewasa dan jantan. Nilai konsentrasi tertinggi Vtg ditemukan dalam plasma darah ikan betina dengan tingkat kematangan III (87,34 mg/mL) kemudian pada tahap II (74,83 mg/mL), I (68,58 mg/mL) dan IV (33,45 mg/mL). Hal ini menunjukkan bahwa pada ikan betina dewasa terjadi proses pembentukan kuning telur (vitelogenesis). Rata-rata diameter telur adalah 0,107 ± 0,052 mm, 0,318 ± 0,086 mm, 0,864 ± 0,099 mm dan 1,041 ± 0,058 mm pada tingkat kematangan I, II, III dan IV secara berurutan. Peningkatan nilai diameter telur seiring dengan perkembangan tahap kematangan gonad menunjukkan bahwa perkembangan telur terjadi karena proses vitelogenesis dan penambahan bahan kuning telur pada oosit. Kata kunci : diameter telur, 17ß-estradiol, kematangan gonad, patin siam, vitelogenin
PRODUCTION PERFORMANCE OF EEL ANGUILLA BICOLOR BICOLOR WITH THE ADDITION OF CALSIUM CARBONAT Saputra, Ardyen; Budiardi, Tatag; Supriyono, Eddy
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3114.46 KB) | DOI: 10.19027/jai.15.56-62

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ABSTRACT Eel have high enough prospects to cultivated. The sustainability of eel culture and overcome slowly growth arising in this cultivation was needed enough information on their biology at growth. One of the aspects that needs to be examined in an effort to increase the growth is the water quality of culture media. In addition to regulate salinity may also be convened by arranging levels of calcium and alkalinity. Calcium is an essential mineral essential required in considerable amount. The objective of the study was to determining the optimal media levels of calcium and alkalinity that can support the survival and growth of eel Anguilla bicolor bicolor and evaluate the role of calcium and alkalinity. The study was conducted with an experimental method with four treatments, the addition of dose CaCO3 on the culture media were (0, 50, 100, 150 mg/L) and repeated three times. Aquariums for fish maintenance were insulated form in the size of 100×50×40 cm3 and with recirculation system. Insulation serves to separate the filter and maintenance parts. The filter used as a physical, chemical, and biology filters. The parameters measured were the survival rate, growth rate on biomass, feed convertion ratio, oxygen consumption rate, and the employment rate of osmotic. The results of this study showed treatment with 50 mg/L calcium carbonate addition into the culture media was the best treatment for growth rate of 1.77 g/day and optimal feed convertion ratio of 2.27, oxygen comsumption rate of 0.41 mg O2/g/h and osmotic work rate of 0.269 mOsm/L H2O. Keywords: eel Anguilla bicolor bicolor, calcium, growth, resirculation  ABSTRAK Ikan sidat memiliki prospek yang cukup besar untuk dibudidayakan. Untuk menjaga usaha budidaya ikan sidat yang berkelanjutan dan mengatasi pertumbuhan yang lambat, maka diperlukan informasi yang memadai tentang aspek biologinya yakni pertumbuhan. Salah satu aspek yang perlu dikaji dalam upaya meningkatkan pertumbuhan tersebut adalah kualitas air media pemeliharaan. Selain dengan mengatur salinitas dapat juga dilakukan dengan mengatur kadar kalsium. Kalsium merupakan mineral esensial yang dibutuhkan dalam jumlah yang cukup banyak. Tujuan penelitian ini adalah untuk menentukan kadar kalsium karbonat yang dapat mendukung kelangsungan hidup dan pertumbuhan ikan sidat Anguilla bicolor bicolor. Penelitian ini dilakukan dengan metode eksperimental dengan 4 perlakuan yaitu penambahan CaCO3 dengan dosis (0, 50, 100, 150 mg/L) dan diulang sebanyak tiga kali. Wadah yang digunakan berupa akuarium bersekat berukuran 100×50×40 cm3 dengan sistem resirkulasi. Sekat berfungsi untuk memisahkan bagian filter dan bagian pemeliharaan. Filter yang digunakan adalah satu unit filter yang berfungsi sebagai filter fisik, kimia, dan biologi. Parameter yang diamati adalah tingkat kelangsungan hidup, laju pertumbuhan mutlak biomassa, konversi pakan, tingkat konsumsi oksigen dan tingkat kerja osmotik. Hasil penelitian menunjukkan bahwa media pemeliharaan ikan sidat dengan penambahan kalsium karbonat sejumlah 50 mg/L merupakan perlakuan terbaik yang dapat meningkatkan pertumbuhan bobot ikan sidat sebesar 1,77 g/hari serta meminimalkan konversi pakan yaitu 2,27, tingkat konsumsi oksigen sebesar 0,41 mg O2/g/jam dan tingkat kerja osmotik sebesar 0,269 mOsm/L H2O. Kata kunci: ikan sidat Anguilla bicolor bicolor, kalsium, pertumbuhan, resirkulasi 
TOTAL HEMOCYTES, GLUCOSE HEMOLYMPH, AND PRODUCTION PERFORMANCE OF SPINY LOBSTER PANULIRUS HOMARUS CULTURED IN THE INDIVIDUAL COMPARTMENTS SYSTEM Pratiwi, Rifqah; Supriyono, Eddy; Widanarni, .
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.338 KB) | DOI: 10.29244/jitkt.v8i1.13768

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Cannibalism can cause high mortality in a lobster culture. To prevent cannibalism, an artificial shel-ter is needed like the individual compartments system (ICS) to ensure that there is no contact bet-ween lobsters. This study aimed to evaluate the application of ICS on total hemocytes glucose hemo-lymph, production performance, and determine the shape of more effective ICS to minimize stress level of lobster. The treatments used for various application shape of ICS was tubular ICS, triangle ICS, square ICS, and shelter PVC pipes as control. Lobster maintenance was conducted in outdoor for 60 days. The results indicated that physiological responses with square ICS treatment were more effective to reduce stress level compared with other treatments (p<0.05). Application of ICS was more optimal to improved SR (p<0.05) and growth of lobster (p>0.05). Overall, treatment of square ICS was opti-mal related to production performance i.e. SR 88.89±5.24%, SGR 0.61±0.49%/day, with harvest size of total length 137.31±8.11 mm/ind, weight 58.83±4.78 g/ind, and FCR 22.71±1.72. Application of ICS in lobster culture was effective to reduce stress level as indicated from total hemocytes and gluco-se hemolymph, and support optimal production performance. Lobster culture using a square ICS sha-pe was effective to minimize stress level, compared with tubular ICS and triangle ICS.Keywords:   cannibalism, glucose, hemocytes, individual compartments system, lobster, production
OCEANOGRAPHY AND WATER QUALITY CONDITION IN SEVERAL WATERS OF THOUSAND ISLANDS AND ITS SUITABILITY FOR WHITE SHRIMP LITOPENAEUS VANNAMEI CULTURE Effendi, Irzal; Suprayudi, Muhammad Agus; Nurjaya, I Wayan; Surawidjaja, Enang Harris; Supriyono, Eddy; Junior, Muhammad Zairin; Sukenda, .
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (983.48 KB) | DOI: 10.29244/jitkt.v8i1.13912

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The purposes of this study were to determine oceanographic and water quality parameters and their suitability for white shrimp Litopenaeus vannamei culture. The measurements were carried out on dry season in Semak Daun Island, Karya Island, and Panggang Island waters of Thousand Islands, with areas of 315.0, 12.0, and 102.8 ha, water depth average of 4.6 m (0.5-28.1 m), 14.6 m (0.5-26.7 m), and 5.3 m (0.8-13.6 m), mean current water velocity of  12.9, 12.7, and 13.5 cm/second, respectively.  In the study areas, we found a diurnal tidal pattern with high wave in January and July-August.  Based on temperature, salinity, and water density in Semak Daun Island waters, there seemingly occurred a turn over indicating a good water circulation, while in Panggang Island and Karya Island waters tended to have a stratification. Generaly, water qualities in the study areas were in the op-timum range for white shrimp culture, i.e., temperature of 29.6-30.8oC, turbidity of 0.10-1.05 NTU, transparency of 5.8-9.7 m, total suspended solid of <8 mg/L, total dissolved solid of 20-164 mg/L, pH of 6.89-7.22, salinity of 32.2-32.3, dissolved oxygen of 5.8-10.8 mg/L, ammonia of 0.068-0.145 mg/L, nitrate 1.247-2.589 mg/L, and phosphate  of 1.021-2.352 mg/L. Moreover, in Semak Daun Island wa-ters, we found the highest suitability for white shrimp culture due to its better water circulation.Keywords: mariculture, coral reef waters, strait, water current, turnover, stratification.
PRODUCTION OF WHITE SHRIMP LITOPENAEUS VANNAMEI IN FLOATING CAGE SYSTEM WITH DIFFERENT STOCKING DENSITY AT THOUSAND ISLAND STRAIT Fendjalang, Sophia N.M.; Budiardi, Tatag; Supriyono, Eddy; Effendi, Irzal
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.261 KB) | DOI: 10.29244/jitkt.v8i1.12718

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The aim of this study was to analyze the survival rate, growth, and haemolymp glucose level of white shrimp as a physiological responses at floating cage cultured on different stock den-sity, in Seribu Islands Strait. White shrimp used in this study contained approximate weight of 1.0±0.06 g and length of 5.0±0.03 cm from Nusa Karamba Hatchery in Seribu Island. White shrimp was maintained in 90 days, feed with ±36.28 % protein content 4 times each day with feeding rate (FR) of 7%. Water qualities and shrimp growth measurements was conducted every 10 days. Haemolymph glucose sam-pling was performed in the beginning and at the end of the experiment. Result showed that treatment with stock densituy of 250 shrimp/m2 had a better survival rate of 30.33% and feed conversion ratio of 1.13 than other treatments. Phy-siological response in the form of glucose hemolim also showed a better response in the 250 ind./m2 stock density of 41.87 mg/dl indicating that the white shrimp did not experience a sig-nificant stress. Keywords: Litopenaeus vannamei,  sea floating cages, stock density, strait waters, Seribu Islands.
THE ADDITION OF ZEOLITE, ACTIVATED CARBON, CLOVE OIL, AND DIFFERENT SALINITY FOR GLUCOSE RESPONSE AND OSMOTIC PRESSURE ON JUVENILE GIANT PRAWN IN CLOSED TRANSPORT SIMULATION SYSTEM WITH HIGH DENSITY Humairani, .; Supriyono, Eddy; Nirmala, Kukuh
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.896 KB) | DOI: 10.29244/jitkt.v8i1.12749

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Long distance transportation of prawn juvenile usually use a closed system. Indonesian farmers often face  problem in this juvenile transportation such as a low survival rate due to shrimp stress and changes in water quality during transportation. This study was aimed to determine the effect of salt, cloves oil, zeolite, and activated carbon for maintaining water quality in 24 hours juvenile giant prawn simulation transportation at high density. The study was conducted at laboratory scale with a complete randomized design with 4 treatments and 3 replicates. For each treatments, it was added 4.67 µl/L clove oil, 20 g of zeolite, 10 g of activated carbon within 4 (four) different salt concentration i.e., 0 g/L (A), 4.7 g/L (B), 9.4 g/L(C), 14.1 g/L(D) and K (without salt, clove oil, zeolites and activated carbon). The results showed that the addition of 9.4 g/L of salt, 4.67 µl/L clove oil, 20 g of zeolite, and 10 g of activated carbon produced the best results with the highest survival rate by 88±2% at the end of simulation transportation and 82±2.83% after rearing, water quality and physiological respons of shrimp remains in good condition at the simulation transportation and at rearing. Keywords: juvenile giant prawn, salt, high density, physiological responses
TOTAL HEMOCYTE COUNT AND HEMOLYMPH GLUCOSE CONCENRATION RESPONSE OF SPINY LOBSTER PANULIRUS HOMARUS ON RATIO OF SHELTER Djai, Suhaiba; Supriyono, Eddy; Nirmala, Kukuh; Adiyana, Kukuh
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 1 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.756 KB) | DOI: 10.29244/jitkt.v9i1.17923

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This research was conducted to assess the physiological response of the lobster Panulirus homarus for the ratio of the shelters. The method used completely randomized design with two replicates of each treatments with shelter ratio (A) 1 : 5, (B) 3 : 5, (C) 4 : 5, (D) 5 : 5. Weight average for 184 lobsters with the stocking density of 23 lobsters for each treatment was 32.64 ± 0.58 g. The experiment was conducted for 60 days. The lobster was fed with trash fish and acclimatized for 7 days before the experiment. Observations on the physiologycal of every 10 days. The physiological responses that observed were total hemocyte count (THC) and hemolymph glucose concentration. The results showed that 4:5 was the best lobster shelter ratio because it could reduce stress levels. This is indicated by the stable values of THC and hemolymph glucose level during the experiment and supported by the growth of 57.28 ± 0.15 g and survival rate of 91.31 ± 2.60%. Keywords: lobster, Panulirus homarus, ratio, shelter, THC, glucose
BLOOD GLUKOSE RESPONSE OF GIANT GOURAMY (OSPHRONEMUS GAOURAMY, LAC.) TO THE STRESS OF ENVIRONMENTAL TEMPERATURE CHANGES Hastuti, S.; Supriyono, Eddy; Mokoginta, Ing; Subandiyono, .
Jurnal Akuakultur Indonesia Vol. 2 No. 2 (2003): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.367 KB) | DOI: 10.19027/jai.2.73-77

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This experiment was conducted to investigate blood glucose performance of giant gouramy (Osphronemus gouramy, Lac.) to environmental changes. Fish with body weight of about 52,15 g was used in the experiment. A hundred and twenty fish were subjected to stress by moving them to another aquarium containing cooler water for 5 minute before put them back to the origin aquarium. The stress treatments were ? 0°C (A), ?-3°C (B), ?-6°C(C), and ?-9°C(D). Blood glucose was measured at 0, 1, 2, 3, 4 and 5 hours post stress, each for 5 fish. During stress treatment, the survival offish were recorded. To study the role of insulin activation on reducing the stress effects, thirty fish were injected with insulin 2 IU/100 g body weight before subjected them to stressar. Blood glucose level of fish subjected to temperature stress of ?-9°C was the greatest. The blood glucose response to temperature changes was linear, Y = 4,4543 X + 35,553 with R2 = 0,09976. The survival rate of fish was 100% for all treatments. Injected of insulin 2 IU/100 g body weight was able to reduce hyperglycemia that caused by stress. Key words: Blood glucose, giant gouramy, Osphronemus gouramy, stress   ABSTRAK Penelitian ini dilakukan dengan tujuan untuk mengetahui performa glukosa darah ikan gurami (Osphronemus gouramy, Lac.) dalam merespon perubahan suhu lingkungan. Ikan berbobot rata-rata 52,15 g sebanyak 120 ekor diberi stres dengan cara diangkat dan dipindahkan ke suatu wadah yang bersuhu lebih dingin selama 5 menit dan dikembalikan lagi ke wadah mula-mula. Perlakuan stres perubahan suhu dingin tersebut adalah A (? 0°C), B (?- 3°C), C (?-6°C) dan D (?-9°C). Glukosa darah diukur dari 5 ekor ikan pada jam ke 0, 1, 2, 3, 4 dan 5 jam pascastres. Kelangsungan hidup dihitung pada saat perlakuan stres. Untuk melihat peran aktivasi insulin dalam menekan efek stres, ikan sebanyak 30 ekor diinjeksi insulin 2 iu/100 g bobot badan sebelum diberi stres. Kadar glukosa darah ikan gurame yang diberi stres perubahan suhu dingin sebesar ?-9°C mengalami peningkatan paling besar. Respon glukosa darah terhadap stres perubahan suhu tersebut berpola linier Y= 4,4543 X + 35,553, dengan R2 = 0,9976. Perlakuan tersebut menghasilkan kelangsungan hidup yang sama yaitu 100%. Injeksi insulin 2 IU/100 g bobot badan mampu menekan hiperglisemia akibat stres. Kata kunci: Glukosa darah, ikan gurami, Osphronemus gouramy, stres
GROW-OUT OF SPINY LOBSTER PANULIRUS SP. WITH HIGH STOCKING DENSITY IN CONTROLLED TANKS Subhan, Rio Yusufi; Supriyono, Eddy; Widanarni, Widanarni,; Djokosetiyanto, Daniel
Jurnal Akuakultur Indonesia Vol. 17 No. 1 (2018): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3437.352 KB) | DOI: 10.19027/jai.17.1.53-60

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ABSTRACTThe aim of this research was to determine optimum stocking density for growing-out of spiny lobster Panulirus sp. in controlled tanks that conducted for 30 days. The experimental spiny lobsters have the initial average weight of 130.39 ± 0.32 g and initial average total length of 140.70 ± 0.06 mm. This study used completely randomized design with three different stocking densities (KT10: 10 ind/m3; KT18: 18 ind/m3; and KT26: 26 ind/m3) and two replications. The parameters observed in this study included water quality (temperature, pH, salinity, dissolved oxygen, and total ammonia nitrogen), physiological responses (total haemocyte count, haemolymph glucose, and frequency of molt), and production performances, such as growth, specific growth rate, feed conversion ratio, and survival rate. The results showed that the spiny lobster could be reared in high stocking density in controlled tanks. Water quality during the study in each treatment was; temperature 26.56?28.65oC, salinity 29.7?33.6 g/L, pH 7.5?8.5, dissolved oxygen 6.15?6.58 mg/L, and total ammonia nitrogen 0.11?0.34 mg/L. The best stocking densities for spiny lobster was 18 ind/m3 (KT18) with 2.5?3.5×106cells/mL total haemocyte counts, 24.6?28.3 mg/dL haemolymph glucose, and 38.37 ± 3.20% frequency of molt. The final average body weight and length were 145.06 ± 0.42 g and 142.77 ± 0.19 mm, respectively. The survival rate reached 86.11 ± 3.92% with a specific growth rate 0.35 ± 0.01%/day, and feed conversion ratio 7.87 ± 0.31.Keywords: high stocking density, Panulirus sp., physiological responses, productivity.  ABSTRAKTujuan dari penelitian ini adalah untuk menentukan kepadatan terbaik dalam pembesaran lobster laut Panulirus sp. yang dipelihara dalam bak terkontrol selama 30 hari. Lobster laut yang digunakan pada awal penelitian memiliki bobot 130,39 ± 0,32 g dan panjang total 140,70 ± 0,06 mm. Penelitian dilakukan menggunakan rancangan acak lengkap dengan tiga perlakuan kepadatan berbeda, yaitu: 10 ekor/m3(KT10), 18 ekor/m3(KT18), dan 26 ekor/m3(KT26) dan dua ulangan. Parameter uji yang diamati dalam penelitian ini meliputi kualitas air (suhu, salinitas, pH, DO, dan TAN), respons fisiologis (total hemosit/THC, glukosa hemolim, dan frekuensi pergantian kulit), dan kinerja produksi meliputi pertumbuhan, laju pertumbuhan spesifik, rasio konversi pakan, dan tingkat kelangsungan hidup. Hasil penelitian menunjukkan bahwa lobster laut dapat dibesarkan dengan kepadatan tinggi dalam bak terkontrol. Pengukuran nilai kualitas air pada setiap perlakuan selama pemeliharaan adalah suhu berkisar 26,56?28,65oC, salinitas 29,7?33,6 g/L, pH 7,5?8,5, DO antara 6,15?6,58 mg/L dan TAN antara 0,11?034 mg/L. Perlakuan terbaik selama penelitian adalah dengan kepadatan 18 ekor/m3 (KT18) dengan nilai THC berkisar antara 2,5?3,5×106  sel/mL, glukosa hemolim 24,6?28,3 mg/dL,dan frekuensi pergantian kulit 38,37±3,20%. Bobot dan panjang lobster akhir rata-rata pada perlakuan tersebut masing-masing mencapai 145,06 ± 0,42 g, dan 142,77 ± 0,19 mm. Tingkat kelangsungan hidup mencapai 86,11 ± 3,92% dengan laju pertumbuhan spesifik 0,35 ± 0,01%/hari dan rasio konversi pakan selama penelitian adalah 7,87 ± 0,31.Kata kunci: padat pemeliharaan, Panulirus sp., produktivitas, respons fisiologis.  
THE EFFECTIVENESS OF LEMNA PERPUSILLA AS PHYTOREMEDIATION AGENT IN GIANT GOURAMI CULTURE MEDIA ON 3 PPT Marda, Alexander Burhani; Nirmala, Kukuh; Harris, Enang; Supriyono, Eddy
Jurnal Akuakultur Indonesia Vol. 14 No. 2 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3077.813 KB) | DOI: 10.19027/jai.14.122-127

Abstract

ABSTRACT The wasted from feed and feces containt nitrogen and phosphorus can decreased fertility and feability water quality. Lemna perpusilla (duckweed) is prospective to use as an agent of phytoremediation of organic waste and can used as animal feed because it has high protein content. Meanwhile water salinity could be accelerate the growth of giant gourami. The aim of this research was to analyze the ability of L. perpusilla in absorbing nutrients nitrogen and phosphorus in water salinity of 3 ppt. The research was conducted four treatments and three replications. The treatments were A (L. perpusilla and 3 ppt salinity), B (L. perpusilla, 3 ppt salinity and filter), C (L. perpusilla, 3 ppt salinity and aeration), and D (L. perpusilla, 3 ppt salinity, filter and aeration). Experiment were carried in aquaria 50×33×50 cm3 in size with density of gourami fish 150/49.5 L for one month. The results showed that the ability of L. perpusilla to absorb N and P decreased from the beginning of the study due to lack of nutrient source of N and P in the aquaculture media, but increased because the impact of the feeding and  metabolism of the gourami. There was no different treatment effect for decreased N and P (P> 0.05). The highest nitrite level was found in D treatment, it means that L. perpusilla not be able to absorb  N and P in the media 3 ppt salinity. However, the addition of 3 ppt salinity gives the best results for the survival rate and feed efficiency ratio. Keywords: phytoremediation, Lemna perpusilla, giant gourami fish, nitrogen and phosphorus  ABSTRAK Limbah pakan dan feses yang mengandung nitrogen dan fosfor dapat menyebabkan penurunan kesuburan dan kelayakan kualitas air. Lemna perpusilla (duckweed) baik digunakan sebagai agen fitoremediasi organik untuk limbah dan dapat digunakan sebagai pakan hewan karena mengandung protein yang tinggi, sementara media bersalinitas mampu mempercepat pertumbuhan ikan gurami. Tujuan dari penelitian ini adalah untuk menganalisis kemampuan L. perpusilla dalam mengabsorbsi nutrisi nitrogen dan fosfor pada air bersalinitas 3 ppt. Penelitian ini terdiri atas lima perlakuan dan tiga ulangan. Perlakuan yang diberikan adalah A (L. perpusilla dan salinitas 3 ppt), B (L. perpusilla, salinitas 3 ppt dan filter), C (L. perpusilla, salinitas 3 ppt dan aerasi), dan D (L. perpusilla, salinitas 3 ppt, aerasi dan filter). Akuarium yang digunakan berukuran 50×33×50 cm3 dengan kepadatan ikan gurami 150 ekor/49,5 L dan waktu pemeliharaan selama satu bulan. Hasil penelitian ini menunjukkan bahwa kemampuan L. perpusilla menyerap limbah N dan P berkurang dari awal penelitian karena kurangnya sumber nutrisi N dan P pada media pemeliharaan, namun beranjak meningkat yang berdampak dari adanya pemberian pakan dan sisa metabolisme dari ikan gurame. Tidak ada perlakuan yang berpengaruh terhadap pengurangan N dan P (P>0,05). Nilai nitrit tertinggi terdapat pada perlakuan D, hal ini berarti bahwa L. perpusilla tidak mampu untuk menyerap limbah N dan P pada media bersalinitas 3 ppt. Namun penambahan salinitas 3 ppt memberikan hasil yang terbaik bagi derajat kelangsungan hidup ikan gurami dan efisiensi pakan. Kata kunci: fitoremediasi, Lemna perpusilla, ikan gurami, nitrogen dan fosfor 
Co-Authors . Subandiyono . SUKENDA . Sulistiono . WIDANARNI ., Berlianti Ahmad Ghufron Mustofa Alexander Burhani Marda, Alexander Burhani Ardi, Idil Ardyen Saputra, Ardyen Bambang Gunadi Budiyanti Budiyanti CECEP KUSMANA Daniel Djokosetianto Daniel Djokosetiyanto Darmansah, Aris Dedi Jusadi Dedi Pardiansyah Diana Sriwisuda Putri Diana Sriwisuda Putri, Diana Sriwisuda Dinamella Wahjuningrum Djai, Suhaiba Eka Rosyida Enang Harris Enang Harris Surawidjaja, Enang Harris Ernik Yuliana Failu, Ismail Fauziyah, Nur Fendjalang, Sophia N.M. Ferdinand Hukama Taqwa Firman, Sri Wahyuni Ghozali, M. Faisol Riza Haris, Enang HARTON ARFAH Hasan, O.D. Subakti Humairani, . I Wayan Nurjaya Iman Rusmana Imron Imron, Imron Ing Mokoginta Irzal Effendi Jr., Muhammad Zairin JULIE EKASARI Koesoemadinata, Santosa Kristanto, Anang Hari Kukuh Adiyana, Kukuh Kukuh Nirmala Lukmini, Aisyah M. Agus Suprayudi M. Toelihere M. Yusuf Arifin, M. Yusuf M. Zairin Junior Mariam, Susanti Mariam, Susanti Mas Tri Djoko Sunarno, Mas Tri Djoko Mia Setiawati Muhamad Yamin MUHAMMAD AGUS SUPRAYUDI Muhammad Hanif Azhar, Muhammad Hanif Muhammad Nabil MUHAMMAD ZAIRIN JUNIOR Munti Yuhana Murie Dwiyaniti, Murie N. Potalangi Ngaddi, Agustinus Novita, Ris Dewi Nurul Taufiqu Rochman ODANG CARMAN Palinggi, Neltje Nobertine Pamungkas, Amin Petrus Rani Pong-Masak Prama, Ega Aditya Pratiwi, Rifqah Puspaningsih, Dewi Rahma Vida Anandasari, Rahma Vida Rahmawati, Riani Riandini, Riandini Ridwan Affandi Rio Yusufi Subhan Rochman, Nurul Taufiqu Taufiqu S. Hastuti Saputra, Adang Saputra, Adang Saputra, Henry Kasmanhadi Setijaningsih, Lies Siburian, Arif Faisal Sukenda Sukenda Sukenda, nFN Surawidjaja, Enang H. Suseno, Sugeng H. Suseno, Sugeng Hari Sutrisno sutrisno Syahputra, Ruspindo Tatag Budiardi Thesiana, Lolita Thomas Nugroho Tri Heru Prihadi Usman Usman Utomo, Bambang Priyo Wahyu Pamungkas Wahyu, nFN Warlina, Lina Warlina, Lina Wasjan, Wasjan Widanarni . Widanarni, Widanarni, Widiyati, Ani Widiyati, Ani Wildan Nurussalam, Wildan Wiramiharja, Yoyo Wiramiharja, Yoyo Yani Hadiroseyani Yosmaniar, nFN Yosmaniar, Yosmaniar Yuni Puji Hastuti