Pipih Suptijah
1Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Kampus IPB Dramaga, Jalan Agatis No. 1, Bogor 16680 Jawa Barat Telepon (0251) 8622909-8622906, Faks. (0251) 8622907

Published : 57 Documents
Articles

PEMBERIAN KITOSAN DAN PENGARUHNYA TERHADAP BERAT BADAN DAN KADAR TRIGLISERIDA DARAH TIKUS SPRAGUE-DAWLEY YANG DIBERI PAKAN ASAM LEMAK TRANS Ali, Abdurrahman; Amalia, Leily; Suptijah, Pipih
Jurnal Gizi dan Pangan Vol. 10 No. 1 (2015)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.844 KB) | DOI: 10.25182/jgp.2015.10.1.%p

Abstract

ABSTRACTThis study was aimed to analyze the effects of chitosan intake on body weight and plasma triglyceride level in Sprague-dawley rats fed by trans fatty acid. This study was an experimental on animal using factorial randomized with post-test only control group design. The samples were 28 male Sprague-dawley rats aged two months old. The samples were divided into one control (K) and three treatment groups which received chitosan as much as 0.035 g (P1), 0.045 g (P2), and 0.055 g (P3). The feed was high-fat diet, namely added by melted margarine as a source of trans fatty acid, as much as 1.7 g per day. Intervention was done for 14 and 28 days. Measurement of body weight was done every three days, whereas measurement of triglyceride level was done after 14 and 28 days intervention. The study revealed that feeding of melted margarine in a dose of 1.7 g per day increased body weight of control group as much as 54% after 28 day intervention. The increase was higher than P1, P2, and P3 groups which were elevated by 48.7%, 27.8%, and 38.9% respectively. In addition, after 14 day intervention, the plasma triglyceride levels of control and P1 groups were categorized as hypertriglyceridemia, namely 254.3±49.0 mg/dL and 241.7±58.5 mg/dL respectively. In the meanwhile, the plasma triglyceride levels of P2 (128.3±14.3 mg/dL) and P3 (141.3 ± 25.3) were significantly lower than the control and P1 groups. After 28 day intervention, there is no significant difference on plasma triglyceride level among groups (p>0.05), but the triglyceride level of each group was significantly lower than the levels after 14 day intervention (p<0.05).Keywords: body weight, chitosan, trans fatty acids, triglycerideABSTRAKPenelitian ini bertujuan menganalisis pengaruh pemberian kitosan terhadap berat badan dan kadar trigliserida plasma tikus Sprague-dawley yang diberi pakan asam lemak trans. Penelitian ini merupakan experimental study pada hewan percobaan, menggunakan rancangan kelompok faktorial dengan post test only control group. Sampel penelitian adalah 28 ekor tikus Sprague-dawley jantan berusia dua bulan, dikelompokkan menjadi satu kelompok kontrol (K) dan tiga kelompok perlakuan (P) dengan penambahan kitosan 0,035 g (P1), 0,045 g (P2), dan 0,055 g (P3) dalam diet yang diberikan. Pakan merupakan diet tinggi asam lemak trans, yaitu dengan penambahan margarin yang dipanaskan sebanyak 1,7 g per hari. Intervensi dilakukan selama 14 dan 28 hari. Berat badan ditimbang setiap tiga hari sementara pengukuran kadar trigliserida dilakukan setelah 14 dan 28 hari perlakuan. Penambahan margarin 1,7 g per hari menjadikan berat badan tikus kelompok kontrol (tanpa kitosan) meningkat 54% setelah 28 hari intervensi, lebih tinggi dibandingkan kelompok P1, P2, dan P3 yang masing-masing mengalami kenaikan berat badan 48,7%, 27,8%, dan 38,9%. Setelah 14 hari intervensi, kadar trigliserida plasma tikus kelompok kontrol dan P1 mengalami hipertrigliseridemia, yaitu masing-masing 254,3±49,0 mg/dL dan 241,7±58,5 mg/dL. Sementara itu, kadar trigliserida plasma tikus kelompok P2 (128,3±14,3 mg/dL) dan P3 (141,3 ± 25,3) lebih rendah dan berbeda signifikan dibandingkan kelompok kontrol dan P1. Setelah 28 hari intervensi, kadar trigliserida plasma tikus tidak menunjukkan perbedaan signifikan antar kelompok (p>0,05), tetapi menunjukkan hasil yang lebih rendah dan berbeda signifikan dibandingkan kadar trigliserida setelah 14 hari (p<0,05).Kata kunci: asam lemak trans, berat badan, kitosan, trigliserida
CHARACTERIZATION OF NANOHYDROXYAPATITE FROM TUNA’S THUNNUS SP BONE AS BIOMATERIALS SUBSTANCE Hanura, Aditya Bayu; Trilaksani, Wini; Suptijah, Pipih
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v9i2.19296

Abstract

Tuna Thunnus sp is a high-value fisheries products. The abundance of waste from tuna?s processing industry has not been used optimally. The objective of this study was to produce and characterize nanohidroxyapatite (Ca5(PO4)3((OH)0.8F0.2) of tuna?s Thunnus sp bone waste using high temperature sintering of 400-800OC continuing by nanomilling method. The results of the characterization showed that the sintering temperature of 800OC was the best treatment yielding of hidroxyapatite 57.73%, 89.27% white degree level. The result of statistic analysis showed that the sintering temperature variation gave a significant effect on the white degree at the test level (p <0.05). Proximate result after sintering showed moisture content of 0.15%, 99.83% ash, 0.05% fat, 0.73% protein and 0% crude fiber. Results of heavy metal analysis showed the content of lead (Pb) and mercury (Hg) was negative, and cadmium (Cd) 0.018 ppm. The mineral test consist of calcium, phosphor, kalium, magnesium, iron, zinc, manganese, and sodium with the dominant elements were 49.76% calcium and 9.02% phosphor. The result of statistic analysis showed that the sintering temperature variation gave significant effect to the three main minerals at the test level (p<0.05). The Particle Size Analyzer test showed that nanohidroxyapatite particles was measured at 800-900 nm. The X-Ray Diffractometer analysis showed the results of sample identification contains hydroxyapatite and fluorian, with syn 100.00%. The hidroxyapatite was a compound complex and crystals formed has a hexagonal shape with a percentage of 82.9% crystallinity. Keywords : bone, nanohidroxyapatite, particles, sintering, tuna
PENENTUAN STRUKTUR MOLEKUL KOLAGEN SISIK IKAN KAKATUA (SCARUS SP) BERDASARKAN SERAPAN MOLEKUL TERHADAP GELOMBANG FTIR (FOURIER-TRANSFORM INFRARED SPECTROSCOPY ANALYSIS) Mberato, Shellyn Prastisia; Rumengan, Inneke F M; Warouw, Veibe; Wullur, Stenly; Rumampuk, Natalie D T; Undap, Suzanne L; Suptijah, Pipih; Luntungan, Aldian Hein
JURNAL PESISIR DAN LAUT TROPIS Vol 8, No 1 (2020): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.8.1.2020.27285

Abstract

 Parrot fish (Scarus sp) is a commodity which commonly consumed in North Sulawesi. High consumption of this fish has caused the high amount of fish scales as wastes. As parrot fish scales contain protein that could be transformed into commercial products such as collagen. Collagen could be applied in the industrial fields including cosmetics and pharmaceutics.  The purpose of this study was to determine molecular structure of collagen derived from the wet and dry parrot fish (Scarus sp) scales, based on molecular absorption of electromagnetic in the infrared region of the fourier transform infrared spectroscopy.Preparation of collagen of fish scales both in wet and dry forms, was initially performed with pre-treatment of raw materials by maceration in sodium hydroxide (NaOH) solution for 48 hours. Then hydrolysis process was conducted in hydrochloric acid (HCl) solution again for 48 hours to remove mineral contents of the scales.  Collagen yield of fish scales in wet and dry forms was 2.23% and 3.00%, respectively, with pH 7, and the respective  water content was  13% and 12%. For collagen derived from the wet scales, the functional groups of amide A and B absorb the electromagnetic at infrared region of 3429 cm-1 and 2930 cm-1), respectively. Also amide I, II and III absorb the electromagnetic at infrared region of 1657 cm-1, 1452 cm-1 and 1242 cm-1, respectively. It was comparable to that of collagen derived from the dry scales, the functional groups of amide A and B absorb the electromagnetic at infrared region of 3425 cm-1 and 2910 cm-1), respectively. Also amide I, II and III absorb the electromagnetic at infrared region of 1653 cm-1, 1402 cm-1 and 1244 cm-1, respectively.  The amide  III group of  the wet scales derived collagen as well as the dry scales derive collagen absorb the electromagnetics at infrared region in the range of 1309-1229 cm-1 indicating that the fish scale derived collagen has not denatured yet, but still in triple helix structure. Molecular functional groups detected for the parrot fish scales derived collagen are in the range of those for  collagen standard.Keywords : fish scale, Scarus sp, collagen, molecule structure, proximate  AbstrakIkan kakatua (Scarus sp) merupakan salah satu jenis komiditi ikan yang banyak dikonsumsi di Sulawesi Utara. Tingginya konsumsi ikan kakatua berakibat banyaknya limbah kuliner ikan ini berupa sisik ikan. Padahal sisik ikan kakatua mengandung protein yang dapat ditransformasikan menjadi produk samping komersial seperti kolagen. Kolagen dapat diaplikasikan pada bidang industry kosmetik dan farmasika. Tujuan penelitian ini menentukan struktur molekul kolagen dari sisik ikan kakatua (Scarus sp) berdasarkan wilayah serapan gelombang infra red.Preparasi kolagen dari sisik ikan baik dalam bentuk basah maupun kering,  diawali dengan proses pre-treatment bahan baku dengan melakukan perendaman menggunakan larutan NaOH selama 48 jam. Selanjutnya adalah tahap hidrolisis yang dilakukan dengan perendaman sampel menggunakan larutan asam klorida (HCl) selama 48 jam untuk menghilangkan mineral yang ada dalam sisik. Kolagen sisik basah dan sisik kering dari ikan kakatua memiliki nilai rendemen masing-masing sebesar 2.23% dan 3.00%, nilai pH 7 serta kadar air sebesar 13% dan 12%. Pada kolagen sisik basah terdeteksi Amida A mempunyai bilangan gel (3429 cm-1), Amida B (2930 cm-1). Amida I (1657 cm-1), Amida II (1452 cm-1 ) dan Amida III (1242 cm-1), sedangkan pada kolagen sisik kering  terdeteksi Amida A mempunyai bilangan gel (3425 cm -1 ), Amida B (2910 cm-1 ). Amida I (1653 cm-1 ), Amida II (1402 cm-1 ) dan Amida III (1244 cm-1). Amida III pada kolagen sisik basah dan kolagen sisik kering terdeteksi pada wilayah serapan 1309-1229 cm-1 hal menandakan bahwa kolagen sisik  ikan kakatua belum terdenaturasi karena masih terdapat struktur triple helix. Gugus fungsional kolagen sisik kering dan kolagen sisik basah dari ikan kakatua memenuhi standar gugus fungsional kolagen standar.Kata kunci : sisik, Scarus sp, kolagen, gugus fungsi, proksimat
CHARACTERISTICS OF DAGGERTOOTH PIKE CONGER FISH (MURAENESOX CINERUS) AS RAW MATERIAL FOR DIVERSIFICATION PRODUCTS DEVELOPMENT Laksono, Untung Trimo; Nurhayati, Tati; Suptijah, Pipih; Nur?aenah, Nani; Nugroho, Teguh Setyo
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 1 (2019): Jurnal Pengolahan Hasil Perikanan
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1230.342 KB) | DOI: 10.17844/jphpi.v22i1.25878

Abstract

Daggertooth pike conger fish (Muraenesox cinerus) has elongated body shape similar to eels and distribute widely worldwide. The fish has edible portion of 50-60% but there has been less information regarding the chemical characteristics. The aim of this research was to determine chemical composition of daggertooth pike conger fish (proximate, amino acid, fatty acid and mineral) and textural properties from its kamaboko. Purposive sampling method applied in this research with length, freshness and distance from location as criteria. Analytical method performed including proximate, amino acids, fatty acids, and minerals (P, Na, Ca, K, Mg dan Fe). Chemical composition from daggertooth pike conger fish showed that on proximate levels of protein, fat, ash, carbohydrates and moisture content value were 12.273%, 4.96%, 1.17%, 1.12% and 80.49% respectively. Dominant macro minerals, namely potassium (K) 170.20 mg /100 g, Ca 90.75 mg /100 g, and Na 80.15 mg /100 g. Amino acid (AA), namely glutamic acid 2.68%, 1.57% lysine, leusine 1.25%, 1.54% aspartic acid and arginine 1:04%. Moreover, the essential fatty acids namely EPA (eicosa pentanoat acid) 0.6%, DHA (docosa hexanoat acid) 0.9%, 0.2% fatty acids linoleic, oleic and linolenic 1.4% 1.0%. CaCl2 (0.2-0.6%) addition to daggertooth pike conger kamaboko could improve textural quality.
The Effects of Nanochitosan Coating to Phenolic Compounds of Basil Leaf (Ocimum basilicum L) Extracts Luntungan, Aldian H.; Mandey, Lucia C.; Rumengan, Inneke F. M.; Suptijah, Pipih
Jurnal Ilmu dan Teknologi Pangan Vol 5, No 2 (2017)
Publisher : Pascasarjana Unsrat

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Abstract

Basil leaf (O. basilicum L) could be developed as a herbal supplement  due to its phenolic compounds which have antioxidant activity. Extract of this plant would be more effective as a natural antioxidant if it is coated with nanochitosan. Nanochitosan was prepared from the parrot fish (scarus sp) scale and chitosan of crab’s shell by gelatin ionic method. The purpose of this study was to determine  the effects of the nanochitosan coating on phenolic content of basil leaves. The parameters tested were phenolic content of  the basil leaf extracts and nanochitosan coated basil leaf extracts. The results show that the  size’s average of nanochitosan  from fish scales were in 657,4 nm. Phenolic compound was not detected in both the fishscale and crab’s shell derived chitosan. In the other hand, phenolic compound of the basil leaf extract  showed the presence of phenol content of 34.38 mg/kg. In basil leaf extracts coated with nanochitosan from fish scales and nanochitosan of crab’s shell showed a phenolic content of 39.28 mg / kg and 57.44 mg/kg respectively. This suggests that synthesis of phenolic compound was induced when the extract of basil leaves was coated by nanochitosan.Keywords: basil leaves, chitosan, nanoparticles, phenolic compounds
Antioxidants Activity of Avocado (Persea americana Mill.) Seeds Extract Coating by Nanochitosan Anggraeny, Dyta; Rumengan, Inneke F. M.; Djarkasi, Gregoria S. S.; Suptijah, Pipih
Jurnal Ilmu dan Teknologi Pangan Vol 5, No 2 (2017)
Publisher : Pascasarjana Unsrat

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

An increasing production of avocado in Indonesia  has resulted in abundant seeds as wastes. Avocado’s seeds contain potential antioxidant compound, such as phenolic compound, which could be exploited for human consumption. Utilization of seeds as functional food could be more efficient by coating with a certain substance. Chitosan is a bio-compatible and non-toxic compound, and could be used as coating agent in the form of nanochitosan. The purpose of this study was to evaluate the content of  phenolic-compound in extract of avocado’s seed with and without coated by nanochitosan. In this study, the extract of avocado’s seed was coated with  Parrot fish scale (Scarus sp) derived nanochitosan, compared with nanochitosan from crab’s shell. Nanochitosan was prepared from chitosan by gelation ionic method. The results show that avocado’s seed extract has 44.89 mg/Kg of phenolic content,  much lower than the content in the extract coated with nanochitosan of fish and crab shell, which were 84.08  and 113,67 mg/Kg, respectively.   Therefore, coating with nanochitosan was proven increasing the phenolic compound in the extract of avocado’s seed.  Keywords: avocado, nanochitosan, phenol
KANDUNGAN KIMIA DARI SISIK BEBERAPA JENIS IKAN LAUT Talumepa, Anggun C. N.; Suptijah, Pipih; Wullur, Stenly; Rumengan, Inneke F. M.
JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Vol 3, No 1 (2016)
Publisher : JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI

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Abstract

Konsumsi ikan laut sangat diminati oleh masyarakat Indonesia, termasuk di Sulawesi Utara. Berlimpahnya restoran seafood yang menyediakan ikan laut sebagai menu utamanya dan penjualan ikan laut di pasar turut meningkatkan produk samping dari ikan seperti sisik yang kurang dimanfaatkan secara optimal. Untuk meningkatkan nilai guna dari sisik ikan laut sebagai suatu produk sampingan perikanan, maka perlu adanya riset untuk mengetahui komposisi kimia yang terkandung dalam sisik ikan laut sebagai upaya menyelidiki potensi sisik untuk dapat dijadikan sumber biomaterial yang dapat berguna bagi manusia. Penelitian ini menggunakan sisik ikan yang berasal dari perairan laut yaitu ikan kakatua, kakap merah, napoleon, salem, dan sahamia. Sejumlah 50 gram sisik dari setiap jenis ikan laut yang dikumpul dianalisis proksimat untuk mengetahui kadar-kadar kimia di dalamnya seperti air, abu, lemak, protein, dan karbohidrat. Kandungan sisik ikan laut yang sudah dikeringkan secara umum adalah air 11%, abu 39%, lemak 5%, protein 30%, dan karbohidrat 15%.
KARAKTERISTIK FISIKOKIMIA KOLAGEN LARUT ASAM DARI KULIT IKAN PARANG-PARANG (CHIROCENTRUS DORAB) Safithri, Mega; Tarman, Kustiariyah; Suptijah, Pipih; Widowati, Neni
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 3 (2019): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.307 KB) | DOI: 10.17844/jphpi.v22i3.28924

Abstract

Waste of parang parang fish (Chirocentrus dorab) skin can be used as a source of collagen. Collagen isolation can be done chemically by the Acid Soluble Collagen (ASC) method. The objective of this research was to isolate collagen with ASC method and characterize their physicochemical. Collagen isolation consisted of pretreatment and hydrolysis with acids. The pretreatment used NaOH 0.1 M for 12 hours, while hydrolysis used acetic acid 0.5 M. Pretreatment results indicated that the concentration of non-collagen protein was 0.1243 mg/mL, while the yield collagen was 2.61%. The collagen had the viscosity of 6.50 cP, the denaturation temperature of 4°C, the transition temperature of 77.30°C, and the melting temperature of 153.90°C. The obtained collagen also had pH of 6.25. The fourier transform infrared (FTIR) spectra analysis showed the collagen contained amide A (3425.58), B (2924.09), I (1647.21), II (1543.05), and III (1246.02) (cm-1). The collagen also contained glycine (26.69%), proline (12.24%) and alanine (9.51%).
CHITOSAN IS A NATURAL MATERIAL EXTRACTED FROM DEACETYLATED CHITIN OF CRUSTACEAN SHELL USED FOR MANY KINDS OF FUNCTIONS, NOT ONLY FOR FOOD BUT ALSO NON FOOD PRODUCT. THE RESEARCH OBJECTIVE IS TO FIND OUT THE EFFECT OF CHITOSAN USED FOR FILLER WITHIN TRANSPARENCY SOAP TO THE CHARACTERISTIC OF PHYSICAL, CHEMICAL AND BIOEFFECT OF END PRODUCT, AND TO FIND OUT WHICH CONCENTRATE OF CHITOSAN WILL BE USED FOR THE BEST PRODUCT. THE RESEARCH WAS DIVIDED BY TWO STEPS, PRE-RESEARCH AND MAIN RESEARCH.  PRE-R Ibrahim, Bustami; Suptijah, Pipih; Amin, Hijrah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 13 No. 1 (2010): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v13i1.3140

Abstract

Chitosan is a natural material extracted from deacetylated chitin of crustacean shell used for many kinds of functions, not only for food but also non food product. The research objective is to find out the effect of chitosan used for filler within transparency soap to the characteristic of physical, chemical and bioeffect of end product, and to find out which concentrate of chitosan will be used for the best product. The research was divided by two steps, pre-research and main research.  Pre-research resulted that transparency soap with 5% chitosan was the best choice based on organoleptic test which was the best in toughness, and based on chemical also microbiological analyses. The soap with 5% chitosan has TPC (Total Plate Count) amount to  2.0x101, while the one without chitosan contained TPC 6.5x101. The main research showed that transparency soap with 5% chitosan has better quality compare to commercialized one.
HYDROXYAPATITE PRODUCTION FROM CUTTLEBONE AS BONE SCAFFOLD MATERIAL PREPARATIONS Henggu, Krisman Umbu; Ibrahim, Bustami; Suptijah, Pipih
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 1 (2019): Jurnal Pengolahan Hasil Perikanan
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2244.688 KB) | DOI: 10.17844/jphpi.v22i1.25869

Abstract

The increasing production of cuttlefish has been associated with the increasing of by-product waste particularly cuttlebone. Cuttlebone is known to contain an inorganic element in form of calcium carbonate(CaCO3) which can be utilized as a source of calcium oxide (CaO) for hydroxyapatite synthesis. This study was aimed to determine the physicochemical characteristics of the cuttlebone and the optimum calcination temperature for CaO extraction and hydroxyapatite synthesis. This study was divided into three steps. Firstly, analysis of the cuttlebone physicochemical properties; secondly, extraction and characterization of the CaO with different calcination temperature (500°C, 600°C, 700°C for 6 hours); and thirdly, hydroxyapatite synthesis using a combination of hydrothermal method at 200°C 6 hours and different calcination treatments (800°C, 900°C, 1,000°C for 1 hour). The results showed that the cuttlebone contained moisture 3.54±0.11%,lipid 0.32±0.19%, protein 4.78±0.23%, carbohydrate 5.29±0.02%, and ash 89.61±0.26. The main element of the ash was CaCO3 aragonite characterized by the high absorption at wavelengths of 1,795; 1,507;1,083; 871; 713 and 700 cm-1. The calcination treatment of 700°C produced the highest amount of CaO. The hydroxyapatite produced with a combination of hydrothermal and calcination temperature 1,000°Chad calcium phosphate ratio (Ca/P) 1.66, crystalline level 90.10%, amorphous level 9.90% and particles morphology of rod-shaped.
Co-Authors . Nurjanah . Prantommy A. Alhana, A. Abdurrahman Ali Aditya Yudha Prawira Sukarno Adjani, Zhalindri Noor Agoes M. Jacoeb Agoes Mardiono Jacoeb Agung, Bagus Sukma Ahmad Zahid Andakke, Jeszy Novianti Anggraeny, Dyta Anjarsari, Anjarsari Asadatun Abdullah Ayu Fitri Izaki Bambang Murdiyanto Basuki Wasis Bustami Ibrahim Dadi Rochnadi Sukarsa Darma Sri Wahyuni Dery Firdaus Desie Rachmania Devi, Hilda Lu?lu?in Nanda Alfira DEWI APRI ASTUTI Didin Komarudin, Didin Dini Indriani, Dini Dini Nurfianti Diniah, Diniah Djoko Poernomo Endang Sri Heruwati Erliza Noor Evy Damayanthi Fajar Kurnia Budiman Gregoria S. S. Djarkasi Gunawan, Febri Hanura, Aditya Bayu Henggu, Krisman Umbu Heni Sri Septiani Hijrah Amin Inneke F. M. Rumengan, Inneke F. M. Irman Febrian JACOB, AGOES M. John Haluan Joko Santoso Kamini, Kamini Kiagus Dahlan Kurniawati Kurniawati Kustiariyah Tarman Laksono, Untung Trimo Leily Amalia Lila Rani LINAWATI HARDJITO lrianto, Hari Eko Lucia C. Mandey Luntungan, Aldian H. Luntungan, Aldian Hein Maggy T. Suhartono Mala Nurilmala MANDELLA, AYU BABY MUTIARA Marina Marina Mberato, Shellyn Prastisia MEGA SAFITHRI Meydia, Meydia Mohammad Saleh Mulyono Baskoro, Mulyono Mursid, Sugara Nainggolan, Hizkia H Y Nani Deviyanti Nisa Nantami Nofri Sandria Novita Sagita, Sri Nugroho, Teguh Setyo Nur?aenah, Nani Pandey, Engel Parapat, Lasma R M E Prinaldi, Wiyan Viyata Pudjiastuti, Sri Ningrum Putri Wening Ratrinia Ratnawulan, Anggun Rezki Kamila Rimbawan Rimbawan Ruddy Suwandi Rumampuk, Natalie D T Rumengan, Inneke F M Saptari Joan Tatra Septembriani, Putri Slamet Hermanto Stenly Wullur Sugeng Heri Suseno Suzanne L Undap SYAHRIAL SYAHRIAL Talumepa, Anggun C. N. TATI NURHAYATI Taufik Hidayat Tetty Kemala Tika Ayu Budiarti Uju Uju Veibe Warouw Wardoyo, Supriyono Eko Widowati, Neni Widyana Ayu Kristantina, Widyana Ayu WIJAYA, MAGGY T Winarti Zahiruddin Wini Trilaksani Wulandari Wulandari Yayandi Gushagia Yugha Subagja Yunisha Aktinidia Zahidah mawardi