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SINTESIS DAN KARAKTERISASI MEMBRAN KOMPLEKS POLIELEKTROLIT (PEC) KITOSAN-PEKTIN Ayuni, Ni Putu Sri; Siswanta, Dwi; Suratman, Adhitasari
Prosiding Seminar Nasional MIPA 2014: PROSIDING SEMINAR NASIONAL MIPA UNDIKSHA 2014
Publisher : Prosiding Seminar Nasional MIPA

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Abstract

Membran PEC kitosan-pektin telah berhasil disintesis. Membran PEC kitosan-pektin dapat di sintesis melalui pencampuran pektin yang dilarutkan dalam air kemudian ditambahkan dengan kitosan dan asam asetat (CH3COOH) 0,4 M. Larutan membran kitosan-pektin diuapkan pada suhu 70 C selama 6 jam kemudian dilepas menggunakan NaOH 1 M. Membran PEC kitosan-pektin dibuat dengan konsentrasi 0,5 %. Variasi membran yang disintesis dengan perbandingan kitosan-pektin (70:30), (80:20), (90:10). Membran PEC kitosan-pektin yang dihasilkan di uji serapan air dan di karakterisasi dengan spektrofotometer inframerah (IR), tarik-regangan, dan Scanning Electron Microscopy (SEM). Hasil spektra IR telah membuktikan terbentuknya membran PEC kitosan-pektin dengan adanya interaksi gugus amina pada kitosan dan karboksil pada pektin bilangan gelombang 1604,77 nm-1. Hasil uji serapan air, tarikan dan regangan untuk membran PEC kitosan-pektin perbandingan 70:30 (255 %; 29 N/mm2; 20 %), 80:20 (182 %, 17 N/mm2, 28 %), 90:10 (142, 24, 10 %). Berdasarkan penelitian ini membran PEC kitosan-pektin dapat digunakan sebagai salah satu alternatif untuk pengolahan limbah cair maupun dalam bidang kesehatan.Kata-kata kunci: kitosan, pektin, membran polielektrolitAbstract: Chitosan-pectin PEC membranes had been synthesized. Chitosan-pectin PEC membrane can be synthesized by mixing pectin is dissolved in water and then added to the chitosan and acetic acid (CH3COOH) 0.4 M. Solution of chitosan-pectin membrane is evaporated at a temperature of 70 °C for 6 hours and then removed using 1 M NaOH . Chitosan-pectin PEC membranes prepared with a concentration of 0.5%. Variations membranes synthesized with chitosan-pectin ratio (70:30), (80:20), (90:10). Chitosan-pectin PEC membrane resulting in water uptake test and characterization by infrared spectrophotometer (IR), tensile-strain, and Scanning Electron Microscopy (SEM). IR spectra results have proved the formation of chitosan-pectin PEC membrane with the amine group on the interaction of chitosan and pectin carboxyl at 1604.77 nm-1. The test results of water absorption, traction and strain for chitosan-pectin PEC membranes 70:30 ratio (255%; 29 N / mm 2; 20%), 80:20 (182%, 17 N / mm 2, 28%), 90:10 (142, 24, 10%). Based on this study of chitosan-pectin PEC membrane can be used as an alternative for wastewater treatment and in health.
ANALITICAL METHOD VALIDATION OF ANIONIC SURFACTANT SODIUM DODECYL BENZENE SULFONATE (SDBS) IN CATFISH BY UV-VIS SPECTROPHOTOMETRY USING ACRIDINE ORANGE Ratri, Monica Cahyaning; Suratman, Adhitasari; Roto, Roto
ALCHEMY Jurnal Penelitian Kimia Vol 13, No 2 (2017): Alchemy Jurnal Penelitian Kimia
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.13.2.8916.145-165

Abstract

The analytical method development on an anionic surfactant of sodium dodecylbenzenesulfonate (SDBS) in a catfish using spectrophotometer UV-Vis using acridine orange (AO) has been conducted. This research aims to determine the optimum conditions of analysis and to determine validation parameters of sodium dodecylbenzenesulfonate (SDBS) analysis in a catfish. This study was divided into two steps, isolation of sodium dodecyl benzene sulfonate (SDBS) in the catfish with soxhlet extraction and the analysis of SDBS. The analysis of sodium dodecylbenzenesulfonate (SDBS) is based on the formation of ion pair between dodecylbenzenesulfonate (DBS) and acridine orange (AO). The results showed that the analysis can be performed at 499 nm, using ethanol as acridine orange (AO) solvent, the mole ratio of dodecylbenzenesulfonate (DBS) acridine orange (AO) 2:1, and pH 2.97. The parameters of validation had good acceptability as linearity (r) 0.998, limit of detection 0.0343 mg/L   and limit of quantification 0.104 mg/L, precision 0.382 - 1.78 %, sensitivity 4.64 x 104 L mol-1cm-1, and accuracy (82.11 - 100.3 %).
STUDY OF LYSINE AND ALANINE DELIVERANCE THROUGH POLYPYRROLE MEMBRANE Suratman, Adhitasari; Buchari, Buchari; Noviandri, Indra; Gandasasmita, Suryo
Indonesian Journal of Chemistry Vol 4, No 3 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (767.154 KB) | DOI: 10.22146/ijc.21851

Abstract

Electropolymerization processes of pyrrole and the usage of polypyrrole membrane as lysine and alanine deliverance have been studied by cyclic voltammetry technique. Polypyrrole membrane was prepared by electropolymerization processes of pyrrole in water based solvent containing sodium perchlorate as supporting electrolyte. Electropolymerization processes were carried out within potential range of 0-1100 mV vs Ag/AgCl reference electrode and at the scanning rate of 100 mV/s. In this study, lysine and alanine have been used as molecules which could easily be loaded on and released from polypyrrole membrane. The presence of lysine or alanine during electropolymerization process reduced the rate of electropolymerization of polypyrrole. In lysine or alanine transfer processes into polypyrrole membrane, the interaction between polypyrrole and lysine or alanine showed by the curve of E½ oxidation in respect of - log C. It proved that the E½ oxidation shifted to more positive potential showed by the increasing of concentration of lysine or alanine. Beside that, voltammetric responses of lysine and alanine transfered into polypyrrole membrane were found to be Nernstian. The results indicated that polypyrrole could be used as a sensor of lysine and alanine.
PREVENTION OF PROTEIN ADSORPTION ON BARE FUSED-SILICA CAPILLARY BY PEG IN CAPILLARY ZONE ELECTROPHORESIS Suratman, Adhitasari; Waetzig, Hermann
Indonesian Journal of Chemistry Vol 9, No 3 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.169 KB) | DOI: 10.22146/ijc.21507

Abstract

The protein separation was studied in capillary zone electrophoresis for preventing protein adsorption on the capillary wall. ß-lactoglobulin (pI: 4.83-5.4, Mr: 18.4 kDa), cytochrome c (pI: 9.59, Mr: 11.7 kDa) and ß-casein (pI: 4.6, Mr: 24 kDa) were used as protein models. Strong adsorption of the proteins occurred onto the capillary at a pH around their pIs. In order to prevent protein adsorption, PEG (Poly(ethylene glycol)) was investigated as an effective substance to stabilize the proteins native state and coat the bare fused-silica capillary surface. The presence of 32 mg/mL PEG in buffer solution in a pH range of 6.0 to 4.0 was successful to suppress protein adsorption during the separation. It can also be confirmed with the reproducibility of apparent EOF mobility with percentile RSD (Relative Standard Deviation) less than 2% in long-term measurement.   Keywords: PEG, protein adsorption, CZE
The Fermentation of Green Algae (Spirogyra majuscule Kuetz) using Immobilitation Technique of Ca-Alginate for Saccharomyces cerevisiae Entrapment Wibowo, Atmanto Heru; Mubarokah, Lailatul; Suratman, Adhitasari
Indonesian Journal of Chemistry Vol 13, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2584.791 KB) | DOI: 10.22146/ijc.21319

Abstract

A study of batch fermentation of green algae (Spirogyra majuscula Kuetz) from Pengging Lake, Boyolali, Central Java for bioethanol source using immobilization technique of Ca-alginate for Saccaromyces cerevisiae entrapment has been done. The scope of the study emphasized on the best condition for the processes of hydrolysis and fermentation. Concentration of sulfuric acid and hydrolysis time were varied with 0.1, 0.2, 0.3, 0.4 and 0.5 M for 30, 90, 150, 210, 270, 330, 360, 390, 420, and 450 min to obtain the maximum glucose content of UV analysis. Na-alginate : yeast ratio and fermentation time were varied with 1:5, 2:4, 3:3, 4:2 and 5:1 (w/w) for 1, 2, 3, 4, 5, 6 and 7 days. Distillation at 70-80 °C was deployed to purify the fermentation product. The ethanol content in the product was analyzed using gas chromatography-flame ionization detector (GC-FID). The result of study showed that maximum glucose content was obtained 2.1% from 0.2 M sulfuric acid for 6 h of hydrolysis. Maximum ethanol content was obtained 54.1% from 2:4 ratio of Na-alginate : yeast (w/w) for 4 days of fermentation. The study also concludes that immobilization technique of Ca-alginate increase alcohol content compared to without immobilization of green-algae fermentation.
STUDY OF ELECTROPOLIMERIZATION PROCESSES OF PYRROLE BY CYCLIC VOLTAMMETRIC TECHNIQUE Suratman, Adhitasari; Buchari, Buchari; Noviandri, Indra; Gandasasmita, Suryo
Indonesian Journal of Chemistry Vol 4, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (932.1 KB) | DOI: 10.22146/ijc.21864

Abstract

Electropolymerization processes and electrochemical properties of polypyrrole as electroactive polymer have been studied by cyclic voltammetric technique. Pyrrole was electropolymerized to form polypyrrole in water-based solvent containing sodium perchlorate as supporting electrolyte in several pH values. The pH of the solutions were varied by using Britton Robinson buffer. The results showed that oxidation potential limit of electropolymerization processes of pyrrole was 1220 mV vs Ag/AgCl reference electrode. It can be seen that cyclic voltammetric respon of polypyrrole membrane that was prepared by electropolymerization processes of pyrrole at the scanning rate of 100 mV/s was stable. While the processes of pyrrole electropolymerization carried out at the variation of pH showed that the best condition was at the pH range of 2 - 6.   Keywords: polypyrolle, electropolymer, voltammetric technique
Effect of Reducing Agents on Physical and Chemical Properties of Silver Nanoparticles Roto, Roto; Rasydta, Hani Prima; Suratman, Adhitasari; Aprilita, Nurul Hidayat
Indonesian Journal of Chemistry Vol 18, No 4 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.594 KB) | DOI: 10.22146/ijc.26907

Abstract

Silver nanoparticles having uniform size and shape, a diameter range of 10–50 nm, excellent stability, and high zeta potential are always desirable for many applications. The silver nanoparticles were synthesized by chemical reduction method using some reducing agents in a polyvinyl alcohol solution. This study aims at determining the effect of reducing agents on the chemical and physical properties of silver nanoparticles. Ascorbic acid, sodium borohydride, hydrazine, sodium citrate, and glucose were used as reducing agents. Surface Plasmon Resonance (SPR) absorbance, morphology, zeta potential, crystal system, and stability of the products were studied. The results showed that the chemical and physical properties of the colloidal Ag nanoparticles were dependent on the reducing agents. In general, the produced silver nanoparticles have an fcc crystal system with a unit cell of 4.0906–4.0992 Å. The SPR absorbance of the colloids has the peak in the range of 401–433 nm. We found that the colloid of silver nanoparticles prepared by using ascorbic acid has uniform spherical shape, the diameter of about 20 nm, and zeta potential of -10.4 mV. After being stored for one month, the SPR absorbance of the colloid decreased by only 5%. This type of colloidal Ag nanoparticles prepared by using ascorbic acid is expected to be used for chemical sensors, an antibacterial agent, and so on.