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Journal : Borneo Journal of Pharmacy

Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava) Pratama, Mohammad Rizki Fadhil; Suratno, Suratno; Mulyani, Evi
Borneo Journal of Pharmacy Vol 1 No 2 (2018): Borneo Journal of Pharmacy
Publisher : ?Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.118 KB) | DOI: 10.33084/bjop.v1i2.367

Abstract

This study aims to obtain the profile of Thin-Layer Chromatography (TLC) and Ultraviolet-Visible (UV-Vis) spectrophotometry from the ethanol extract of akar kuning stems (Arcangelisia flava) from Central Kalimantan. The TLC method is used with the orientation phase of the combination of polar-non-polar solvents resulting from orientation, while ethanol is used as the solvent for UV-Vis spectrophotometers. TLC results showed the formation of three stains on a mixture of polar solvents chloroform : methanol : water while in a non-polar solvent combination n-hexane : ethyl acetate did not show any stains. Comparison of retention factor (Rf) values shows the best combination of polar solvents to separate stains at a ratio of 5 : 2 : 1, respectively. Separation in 2-dimensional TLC with polar solvents showed a similar pattern with 1-dimensional separation in the form of three stains. UV-Vis spectrophotometer results showed four main peaks with wavelength 227.2; 267.4; 345.2; and 425.3 nm, respectively. The profile of the peak formed is very similar to that shown by berberine, one of the primary metabolites of akar kuning. TLC and UV-Vis spectrophotometers profiles obtained are expected to support further research using akar kuning stems, especially those from Central Kalimantan.
Co-Authors Abdul Kohar Acep Suherman, Acep Adi, Widi Cahya AGUS AGUS Akbariyah, Fitri Al Ifhan, Donni Al Ifhan, Donni Alwan Abdurahman Amandus, Hieronimus Amandus, Hieronimus Amilin Amilin, Amilin Aminuddin HP Aminuddin Prahatama Putra Annisa, Khoirul Ardhany, Syahrida Dian ardina, rinny Arinda, Silke Arya Mahdi Atikno, Welly Baskoro, Pandu Alvi Darmawang, SE., M.Ak., Gilang Dewi, Jiniari Dewi Dian Kurniati Dieni Mansur Djadang, Syahril Djaddang, Syahril Dwi Purbayanti, Dwi Ernungtyas, Niken Febrina Evi Mulyani Fauziah, Riski Febrianti, Lusiana Fera Sartika, Fera Fifi Swandari Fitriawati, Roza Hasna, Safira Helmus, Helmus Hidayat Teguh Wiyono Hildayanti, Hildayanti Husiano, Dwi Ida Zulaeha Iis Nur Asyiah Irwansyah Irwansyah Jekti Prihatin M. Yamin Marchel, Jarot Maulidia, Oktoreza Mochammad Iqbal Mohamad Muspawi MP, Dr. Ir. M. Syarief, Muhammad Ikhwan Rizki, Muhammad Ikhwan Muhammad Reza Cordova, Muhammad Reza Muhammad Yusuf Mujiman Hendri Wijaya Nurhalina Nurhalina, Nurhalina Nurmala Ahmar Nurmalina, Rina Nur’aeni, Yeni Palupi, Ariani Panggabean, Hotma Parulian Pratama, Mohammad Rizki Fadhil Pratama, Mohammad Rizki Fadhil Pratiwi, Sunti A Prisanto, Guntur Freddy Purnadayanti, Zahriza Rachma Puspitasari Rahayuningrum, Novidha Muji Rianti, Rima Rianti, Rima Ridwan Ridwan Rizky, Maulana Saharudin Saharudin San, Setiawan SANDRA, NOVITA Satya, Arnino Adhy Setyawan, Dodi Yudo Shalih, Yusrijal Slamet Santosa Suciati Suciati Sukarsih Sukarsih, Sukarsih Triyoni Purbonegoro, Triyoni Utomo Utomo Utomo, Ronny Victor Wardhani, Dyah Febria Widarto Rachbini, Widarto Yulianti, Eka Budi