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IN VITRO GROWTH AND ROOTING OF MANGOSTEEN (GARCINIA MANGOSTANA L.) ON MEDIUM WITH DIFFERENT CONCENTRATIONS OF PLANT GROWTH REGULATOR HARAHAP, FAUZIYAH; POERWANTO, ROEDHY; SUHARSONO, .; SURIANI, CICIK; RAHAYU, SUCI
HAYATI Journal of Biosciences Vol. 21 No. 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (943.386 KB) | DOI: 10.4308/hjb.21.4.151

Abstract

Propagation of mangosteen is challenging for many reasons, including limited seed set, slow rate of seedling growth, and difficulty with root formations. The objective of this research was to find the best combination of medium and plant growth regulator for in vitro growth and rooting of mangosteeen seed. Various types of explant (a whole seed; seed divided into 2, 3, and 4 cross sections; seed divided into 2, 3, and 4 longitudinal sections) were treated with five concentrations of benzyl amino purine (BAP; 0, 2.5, 5, 7.5, 10 mg/L) for shoot induction in ½ Nitrogen (N) Murashige and Skoog (MS) medium. The shoots were rooted on MS and woody plant medium (WPM) media with several combinations of indole butyric acid (IBA) and naphtalene acetic acid (NAA). Treatments for root induction were applied as follows: (i) low dose, given during induction of rooting, (ii) soaking the base of the shoots in medium treated with a high dose of auxin for 5 days, and then growing the shoots in MS ½ N with 1 mg/L NAA +  1 mg/L BAP medium. Our result show that BAP positively affected mangosteen bud growth. The best medium for mangosteen shoot regeneration was found to be  MS ½ N  + 5 mg/L BAP. This medium induced  the highest number of shoots from the seed explant cut into four cross sections. We found the best medium to induce in vitro rooting of mangosteen shoot was MS ½ N + 3 mg/L indole butiric acid (IBA) + 4 mg/L NAA medium. Some treatment negatively affected growth. Soaking the mangosteen shoot base in a medium with an overly high dose of auxin seemed to disrupt and inhibit growth of the mangosteen shoot.
PERTUMBUHAN TUNAS MANGGIS (Garcinia Mangostana L) IN VITRO HASIL PERLAKUAN ZAT PENGATUR TUMBUH BENZYL ADENIN DAN UKURAN EKSPLAN YANG BERBEDA Harahap, Fauziyah; ., Hasratuddin; Suriani, Cicik
JURNAL PENELITIAN SAINTIKA Vol 12, No 1 (2012): MARET 2012
Publisher : JURNAL PENELITIAN SAINTIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Manggis adalah salah satu buah asli Indonesia yang memiliki potensiekspor sangat besar, namun pertumbuhan tanaman ini sangat lambat. Alternatifuntuk meningkaikan pertumbuhannya yaitu melalui teknik kultur jaringan denganmemanjaatkan zat pengatur tumbuh (ZPT) Benzyl Adenine (BA). Penelitian ini bertujuan untuk mengetahui: 1) Pengaruh konsentrasi ZPT BA terhadappertumbuhan tunas manggis (Garcinia mangostana L.) in vitro, 2) Pengaruhukuran eksplan terhadap pertumbuhan tunas manggis (Garcinia mangostana L.) invitro, 3) Pengaruh interaksi ZPT BA dan ukuran eksplan terhadap pertumbuhan tunas manggis (Garcinia mangostana L.) in vitro. Bahan penelitian yangdigunakan adalah biji manggis dari lapang. Rancangan penelitian ini adalahRancangan Acak Lengkap (RAL) Jaktorial dengan dua Jaktor perlakuan yaitu DosisZPT BA terdiri dari 4 taraf perlakuan, ukuran eksplan terdiri dari 3 tarafperlakuan, sehingga ada 12 kombinasi perlakuan. Eksplan biji disterilisasi dan ditumbuhkan dalam media MS dengan beberapa dosis ZPT BA (0 ppm, 2 ppm, 4 ppm, 6 ppm), dengan 3 ukuran eksplan (utuh, 1 cm, 2 cm). Parameter pengamatanpenelitian ini adalah 1) Waktu munculnya tunas, 2) Jumlah tunas, 3) Jumlah daun,4) Tinggi tanaman. Data dianalisis secara deskriptif dan inferensial denganANA VA RAL Jaktorial. Berdasarkan hasil uji statistik, perlakuan konsenirasi BA(Benzyladenin) dan ukuran eksplan tidak memberkan pengaruh yang nyataterhadap periumbuhan manggis (Garcinia mangostana L.) secara in vitro, padawaktu munculnya tunas, jumlah daun, tinggi tanaman dan juga jumlah tunas(interaksi AB), namun berdasarkan analisis statistic deskriptif, pertumbuhan tunaspada jumlah tunas umur 12 MST, Jaktor A (konsentrasi BA ) memberikanpengaruh terhadap jumlah tunas. Periumbuhan tunas yang paling cepat pada umur 3 MST pada perlakuan konsentrasi 4 ppm dengan ukuran eksplan 2 cm, jumlahtunas paling tinggi dicapai dengan rata - rata 5,99 (4 ppm ukuran 2 cm), jumlahdaun paling tinggi dicapai dengan rata - rata 6,53 ( 4 ppm ukuran utuh) danjumlah daun paling banyak 9 helai (4 ppm ukuran 2 ), sedangkan tinggi tanaman tertinggi dicapai pada perlakuan ( 4 ppm ukuran utuh) yaitu rata - rata 6,29.
Analisis Pembelajaran Biologi dalam Pespektif Inkuiri pada Pembelajaran Menggunakan Kurikulum Kerangka Kualifikasi Nasional Indonesia (KKNI) Brata, Wasis Wuyung Wisnu; Suriani, Cicik
Jurnal Pendidikan Biologi Vol 8, No 1 (2018): Jurnal Pendidikan Biologi
Publisher : Magister Pendidikan Biologi, Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpb.v8i1.12101

Abstract

The application of the new curriculum directs more scientific learning by applying the six task method. However, curriculum changes often have obstacles in their implementation. This study aims to analyze the learning environment in biology classes from the perspective of inquiry learning, as one of the most recommended science learning approaches. The research uses descriptive methods, with a population of 121 students from five environmental biology classes who have fully implemented the IQF curriculum. Samples taken by cluster random sampling method obtained two classes with a total of 46 students. Data obtained from questionnaires and learning outcomes documents. The results showed that biology learning activities had a level of conformity with inquiry learning in the "sufficient" category. Data shows that among the components of inquiry activity observed, questioning and hypothesizing are the lowest intensity activities. While communication activities have the highest intensity. It can be concluded that the learning of environmental biology still needs to be improved, especially in questioning and hypothesizing activities.