Articles

PERBANDINGAN SENYAWA FENOLIK DAN AKTIVITAS ANTIOKSIDAN ANTARA SAGU BARUK SEGAR DAN KERING Momuat, Lidya Irma; Suryanto, Edi; Rantung, Olha; Korua, Aneke; Datu, Hasan
CHEMISTRY PROGRESS Vol 8, No 1 (2015)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.8.1.2015.9399

Abstract

Sago Baruk (Arenga microcarpha) is one of endemic crop type of Archipelago of Sangihe Talaud, North Sulawesi and potential as source of alternative food. The objectives of this research were to determine the phenolics composition and antioxidant activity of fresh and dry sago baruk trunks pith sago which was extracted by sequentially with aquades and filtrate. Flour sago baruk extract were analysed phenolic total, flavonoid and tannin condensed content by spectrophotometer. Antioxidant activity of each sago flour are evaluated in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, total antioxidant and reducing power. Result of analysis in phenolic dan flavonoid total content indicate that dry sago baruk higher compared fresh sago flour of baruk wet while tannin condensed content do not show difference. Result of examination of antioxidant activity indicated that fresh sago baruk having higher DPPH free radical scavenging activity compared to dry sago baruk (the average values of 83,08% and 81,49%, respectively). In contrast, total antioxidant and reducing power of dry sago baruk presented higher antioxidant activity compared to fresh sago baruk with average value (the average values of 109,48 and 92,52 ?mole/100 g, respectively). Futhermore, correlations analysis revealed that total phenolic, flavonoid, tannin content with DPPH and FRAP scavenging activities were well positively correlated with each other (R= 0.7526; 0,7467 and 0,8146, respectively). Result of this research concluded that dry and fresh sago baruk trunks pith having phenolic compound and antioxidant activitiesSagu baruk (Arenga microcarpha) merupakan salah satu jenis tanaman endemik Kepulauan Sangihe Talaud, Sulawesi Utara yang potensial sebagai sumber pangan alternatif. Tujuan penelitian ini adalah untuk menentukan komposisi senyawa fenolik dan aktivitas antioksidan dari sagu baruk segar dan kering yang diekstraksi secara sekuensial dengan akuades dan filtrat. Empelur batang sagu segar dan kering diekstraksi secara sekuensial dengan akuades dan filtrat. Ekstrak tepung sagu baruk dianalisis kandung total fenolik, flavonoid dan tannin terkondensasi dengan spektrofotometer. Aktivitas antioksidan dievaluasi dengan penangkalan radikal bebas difenil pikrilhidrazil (DPPH), total antioksidan dan kemampuan mereduksi. Hasil analisis terhadap kandungan total fenolik dan flavonoid menunjukkan bahwa sagu baruk kering lebih tinggi dibandingkan dengan tepung sagu baruk basah sedangkan kandungan tannin terkondensasi tidak menunjukkan perbedaan. Hasil pengujian aktivitas antioksidan menunjukkan bahwa sagu baruk segar memiliki aktivitas penangkal radikal bebas DPPH lebih tinggi dibandingkan dengan sagu baruk kering (dengan persentase rata-rata berturut-turut adalah 83,08 dan 81,49%). Sebaliknya, total antioksidan dan kemampuan mereduksi sagu baruk kering memberikan aktivitas antioksidan tertinggi dibandingkan dengan sagu baruk segar (dengan rata-rata 109,48 dan 92,52 ?mol/100 g). Selanjutnya, analisa korelasi mengungkapkan bahwa kandungan total fenolik, flavonoid, tannin dengan aktivitas penangkalan DPPH dan FRAP secara positif berhubungan dengan satu sama lainnya (R= 0.7526; 0,7467 dan 0,8146). Hasil penelitian ini menyimpulkan bahwa empelur sagu baruk kering dan segar memiliki senyawa fenolik dan aktivitas antioksidan.
ANALISIS FITOKIMIA ENCENG GONDOK (EICHHORNIA CRASSIPES) DAN EFEKNYA SEBAGAI AGEN PHOTOREDUKSI FE3+ Rorong, Johnly A.; Suryanto, Edi
CHEMISTRY PROGRESS Vol 3, No 1 (2010)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.3.1.2010.72

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Eichhornia crassipes is a weeds to people. The objectives of this research were to analyze phytochemicalcontent and photoreduction activity. Phytochemical content that analyzed were total phenolic, total flavonoidand condenced tannin. Photoreduction activity were done using reduction of Fe3+ to Fe2+. The highest totalphenolic, flavonoid and condenced tannin were extracted with water in leaves, 26.327, 3.191, 25.300 mg/kgrespectively. Photoreduction activity of enceng gondok decreases Fe3+ concentration from 9,111 to 1,500 ppm.Enceng gondok posses photoreduction activity because it has phenolic, flavonoid and condenced tannincontent.
AKTIVITAS ANTIFOTOOKSIDASI DAN FOTOPROTEKSI FRAKSI PELARUT DARI DAUN GEDI (ABELMUSCHUS MANIHOT L) Abigael, Reliana; Suryanto, Edi; Katja, Dewa G.
CHEMISTRY PROGRESS Vol 12, No 1 (2019)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.12.1.2019.27914

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ABSTRAK Penelitian ini bertujuan untuk menentukan aktivitas antifotoksidasi mengukur nilai SPF dari ekstrak metanol daun Gedi (Abelmoschus manihot L). Daun gedi diekstraksi dengan cara sokletasi.  Dalam penelitian ini sampel diekstraksi dengan pelarut metanol, difraksinasi dengan pelarut petroleum eter, etil asetat, butanol dan akuades. Dari hasil fraksinasi tersebut kemudian diuji aktivitas antifotooksidasi dan fotoprotektif. Kandungan fenolik menggunakan asam galat sebagai larutan standart. Uji Aktivitas antifotooksidasi ditentukan dengan menghitung nilai diena terkojugasi yang merupakan pembentukan awal oksidasi pada lipid. Semakin rendah nila diena terkonjugasi, maka semakin baik sebagai antifotooksidasi. Untuk penentuan fotoprotektif, dilakukan dengan mneggunakan spektrofotometer UV-Vis pada panjang gelombang 290-320 nm dengan konsentrasi 100 ?g/mL. Hasil penelitian ini menunjukkan bahwa fraksi etil asetat memiliki kandungan fenolik, aktivitas antioksidan, antifotooksidasi, dan fotoprotektif yang paling tinggi. Hasil yang diperoleh menunjukkan total fenol paling tinggi pada fraksi eti asetat yaitu 110.204 µg/mL, sedangkan untuk aktivitas antioksidan 57.72%. Hasil pada aktivitas antifotooksidasi menunjukkan bahwa fraksi Etil asetat menunjukkan kemampuan yang paling baik dan untuk fotoprotektik memiliki nilai SPF yang tinggi adalah etil asetat yaitu 9.03.  ABSTRACT This study aimed to determine the antifotoxidation activity measuring the SPF value of the solvent fraction of the leaves of Gedi (Abelmoschus manihot L). Gedi leaves extracted by socletation. In this study the samples were extracted with methanol solvent, fractionated with petroleum ether, ethyl acetate, butanol and aquades. The results of the fractionation were then tested for antifotooxidation and photoprotective activity. Phenolic content uses gallic acid as a standard solution. The antifotoxidation activity test is determined by calculating the conjugated diene value which is the initial formation of oxidation in the lipid. The lower the conjugated diene tilapia, the better it is antifotoxidation. For photoprotective determination, a UV-Vis spectrophotometer was carried out at a wavelength of 290-320 nm with a concentration of 100 ?g / mL. The results of this study indicate that the highest ethyl acetate fraction has the highest phenolic content, antioxidant activity, antifotoxidation and photoprotective. The results obtained showed the highest total phenol in the eti acetate fraction of 110,204 µg / mL, while for antioxidant activity 57.72%. The results of the anti photoxidation activity showed that the ethyl acetate fraction showed the best ability and for the photoprotectic the high SPF value was ethyl acetate namely 9.03.  
AKTIVITAS ANTIFOTOOKSIDAN DAN KOMPOSISI FENOLIK DARI DAUN CENGKEH (EUGENIA AROMATIC L.) Lumingkewas, Meiske; Manarisip, Jeanette; Indriaty, Fetty; Walangitan, Amelia; Mandei, Judith; Suryanto, Edi
CHEMISTRY PROGRESS Vol 7, No 2 (2014)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.7.2.2014.7473

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The objectives of the present research were evaluated effect of solvent extraction on antiphotooxidative from cloveleave (Eugenia aromatic (L). A certain quantity of powdered sample was extracted with methanol 80%, ethanol 80%and aseton 80%. After evaporation the extracts were dissolved in methanol and assayed for phenolic, flavonoid andcondensed tannin content. Singlet oxygen quenching and free radical activity of each extracts of spices at 500 ppmlevel were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. Anti-photooxidative activity test wasconducted using linoleic acid as substrates each containing 5 ppm erythrosine as a photosensitizer. The resultshowed that solvent was significantly having effect on total phenolic, flavonoid and condensed tannin content.Methanol extract possessed highest total flavonoid and condensed tannin content than aseton and etanol. Methanolextract of clove leave was found to have the highest anti-photooxidative effect, followed by aseton and ethanol. Inaddition, ekstrak methanol showed any difference in it free radical scavenger effect than that of ethanol and asetonextracts. This results show that clove methanol extract has the best activity on photooxidation of linoleic acid andscavenger of free radical DPPH. These results suggest that the methanolic extract of clove leave contains somecompounds having singlet oxygen quenching and free radical scavenging activities on linoleic acid and free radicalDPPH.Penelitian ini bertujuan untuk mempelajari pengaruh pelarut ekstraksi terhadap aktivitas antifotooksidan dankomposisi fenolik yang terdapat pada ekstrak daun cengkeh. Serbuk daun cengkeh diekstraksi dengan metanol 80%,etanol 80% dan aseton 80%. Setelah evaporasi, ekstrak daun cengkeh dilakukan pengujian untuk kandungan fenolik,flavonoid dan tannin terkondensasi. Aktivitas penstabilan oksigen singlet dan radikal bebas dari ekstrak dievaluasidengan menggunakan asam linoleat sebagai substrat yang mengandung 5 ppm eritrosin sebagai fotosensitiser danradikal bebas 1,1-diphenyl-2-picrylhydrazyl (DPPH). Hasil penelitian ini menunjukkan bahwa pelarut ekstraksimemiliki efek sangat signifikan terhadap kandungan fenolik, flavonoid dan tannin terkondensasi. Pelarut ekstrakmetanol memiliki kandung flavonoid dan tannin terkondensasi paling tinggi daripada aseton dan etanol. Ekstrakmetanol daun cengkeh ditemukan mempunyai efek antifotooksidatif diikuti ekstrak etanol etanol dan aseton. Selainitu, pelarut methanol juga menunjukkan suatu perbedaan dalam penangkalan radikal bebas DPPH. Berdasarkananalisis komposisi fenolik dalam daun cengkeh dengan HPLC teridentifikasi senyawa eugenol, asam galat, kuersetin,rutin dan kaempferol. Hasil ini menyimpulkan bahwa ekstrak metanol daun cengkeh mengandung beberapasenyawa fenolik yang mempunyai aktivitas sebagai penstabilan oksigen singlet dan penangkalan radikal bebas.
POTENSI ANTIOKSIDAN DAN FOTOPROTEKTIF TEPUNG KOMPOSIT DARI PISANG GOROHO, JAGUNG MANADO KUNING DAN SAGU BARUK Suryanto, Edi; Momuat, Lidya
CHEMISTRY PROGRESS Vol 10, No 2 (2017)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.10.2.2017.27749

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ABSTRAK Pisang, jagung dan sagu merupakan sumber makronutrien, vitamin, mineral, serat pangan dan fitokimia. Tujuan penelitian adalah untuk menentukan aktivitas antioksidan dan fotoprotektif tepung komposit dari pisang goroho, jagung manado kuning dan sagu baruk menggunakan perbedaan pelarut. Tepung komposit diproduksi menggunakan campuran tepung pisang, jagung dan sagu pada perbandingan 60:30:10. Tepung komposit dievaluasi menggunakan kandungan total flavonoid (metode aluminium), aktivitas antioksidan (uji radikal 1,1-diphenyl-2-phycrylhidrazyl) dan aktivitas fotoprotektif (uji SPF menggunakan spektrofotometer). Hasil penelitian ini menunjukkan bahwa kandungan total flavonoid tertinggi ditemukan pada pelarut aseton dibandingkan dengan methanol, ethanol, petroleum eter dan TPTJ (kontrol) pada tepung komposit. Aktivitas penangkal radikal bebas ekstrak aseton tepung komposit menunjukkan aktivitas paling tinggi daripada ekstrak etanol, metanol, petroleum eter dan TPTJ (kontrol). Hasil penelitian ini juga menunjukkan bahwa ekstrak aseton mempunyai aktivitas fotoprotektif paling tinggi daripada ekstrak metanol, etanol, petroleum eter dan TPTJ (kontrol) pada nilai SPF (sun protection factor). Secara keseluruhan, penelitian ini menyarankan bahwa ekstrak aseton tepung komposit mengandung komponen yang memberikan aktivitas antioksidan dan fotoprotekstif serta berpotensi melindungi kulit dari efek radiasi UV-B. Kata kunci: tepung komposit, pelarut, antioksidan dan fotoprotektif ABSTRACT  Banana, corn and sago are source of macronutriens, vitamines, minerals, dietary fiber and phytochemicals. The objective of this study was to determine antioxidant and photoprotective activities of composite flour from goroho banana, yellow Manado corn and baruk sago using different solvent. Composite flour was produced using mixture of banana, corn and sago flour in the ratio of 60:30:10. The composite flour extracted with petroleum ether, acetone, ethanol, and methanol by using a maceration extraction for 24 hours. The composite flour was evaluated using total flavonoid content (aluminium ion method), and free radical scavenging activity (1.1-diphenyl-2-phycrylhidrazyl radical assay). The in vitro photoprotective activity was studied by UV spectrophotometric method in the range of 290-320 nm. This result showed that the highest total flavonoid content was found in acetone solvent compared that of methanol, ethanol, petroleum ether, and TPTJ (control) in composite flour. The radical scavenging activity of acetone extract of flour composite showed the highest activity than ethanol, methanol, petroleum ether, and TPTJ (control). The results also showed that the acetone extract has highest photoprotective activity than methanol, ethanol and petroleum ether in sun protection factor (SPF) value. In overall, the present study suggests that acetone extract of composite flour contains components that give antioxidant and photoprotective activities and potency to protect skin of UV-B radiation effects. Keyword: composite flour, solvents, antioxidant, photoprotective
ULTRASOUND-ASSISTED EXTRACTION ANTIOKSIDAN SERAT PANGAN DARI TONGKOL JAGUNG (ZEA MAYS L.) Suryanto, Edi; Taroreh, Mercy R. I.
CHEMISTRY PROGRESS Vol 12, No 2 (2019)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.12.2.2019.27932

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ABSTRAK Tujuan penelitian ini adalah menentukan pengaruh pelarut dan waktu ekstraksi berbantu ultrasonik dari tepung serat pangan tak larut (SPTL) dari tongkol jagung dan aktivitas antioksidannya. Etanol dan aseton digunakan untuk mengektrak komponen fenolik dri tepung IDF tongkol jagung dengan waktu ekstraksi (30, 60, 90, 120, 150 dan 180 menit) pada dievaluasi berdasarkan kandungan fenolik dan aktivitas antioksidan dari ekstrak tanaman. Hasil penelitian menunjukkan bahwa ekstrak etanol memiliki kandungan fenolik tertinggi dibandingkan dengan aseton ekstrak semua tingkat waktu ekstraksi. Hasil ini juga menunjukkan bahwa ekstrak etanol memiliki aktivitas antioksidan yang lebih kuat dalam aktivitas pemulungan radikal dan mengurangi daya antioksidan. Studi ini menyimpulkan bahwa waktu ekstraksi dan jenis pelarut mempengaruhi total kandungan fenolik dan aktivitas antioksidan. Kata kunci:  ABSTRACT The objective of this study was to determine solvents effect and extraction time of ultrasound-assisted extraction of corncob insoluble dietary fiber (IDF) powder and their antioxidant activities. Ethanol and acetone were used to extract phenolic components from corncob IDF powder with extraction time (30, 60, 90, 120, 150 and 180 min) in an ultrasonic bath (Krisbow 42 kHz, 50 watt). The both extracts were evaluated on the phenolic content and antioxidant activity with spectrophotometry method. The results of the phenolics content expressed in ?g/mL of gallic acid equivalent showed that ethanol extract has highest phenolic content compared with acetone extract of all the extraction time. The radical scavenging activity of extract ethanol exhibited strong than acetone extract of all extraction time tested. The result also showed that the ethanol extract has highest the total antioxidant capacity compared with acetone extract at all extraction time. This result suggest that corncob IDF powder especially the ethanol extract are very rich in phenolic component which is potential as antioxidant dietary fiber.Keyword: corncob, dietary fiber, ultrasonic, extraction time, phenolic, antioxidant
PENGARUH LAMA PERENDAMAN TERHADAP AKTIVITAS ANTIOKSIDAN DARI EMPELUR SAGU BARUK (ARENGA MICROCHARPHA) Momuat, Lidya Irma; Suryanto, Edi
CHEMISTRY PROGRESS Vol 9, No 1 (2016)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.9.1.2016.13909

Abstract

Sago baruk is one of endemic crop type of Archipelago of Sangihe Talaud, North Sulawesi. The objectives of this research were to study immersion time effect on antioxidant activity of sago baruk pith extract. The fresh sago baruk trunks pith was extracted with destilate water and stored at room temperature for 1, 2 and 3 days. After that, the filtrate was evaporated with heating technique. The pith extract was analysed phenolic total content by spectrophotometer. Antioxidant activity of each pith extract are evaluated in 1.1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging (DPPH method), total antioxidant (FRAP method), antioxidant capacity (Phophomolybdate method) and reducing power (potassium ferricyanide method). The result of analysis in phenolic content showed that pith extract at immersion time of first day higher compared with second and third days. The result is in line with free radical scavenging measuring with immersion time of first day higher than second and third days. Likewise, the total antioxidant, antioxidant capacity and reducing power also showed that pith extract in immersion time of first day has highest antioxidant capacity than second and third days. There is effect that immersion time of sago baruk pith influence on phenolic content and antioxidant activity. This result concluded that sago baruk pith extract having phenolic compound and antioxidant activities. There is effect that immersion time of sago baruk pith influence on phenolic content and antioxidant activity. This result concluded that sago baruk pith extract having phenolic compound and antioxidant activities.
AKTIVITAS ANTIFOTOOKSIDASI NANOPARTIKEL PERAK YANG DISINTESIS MENGGUNAKAN KULIT PISANG KEPOK (MUSA PARADISIACA L.) Colling, Erningsi; Suryanto, Edi; Wuntu, Audy
CHEMISTRY PROGRESS Vol 11, No 2 (2018)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.11.2.2018.27943

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ABSTRAK Penelitian ini bertujuan untuk mengetahui Aktivitas antifotooksidasi nanopartikel perak yang disintesi menggunakan kulit pisang kepok. Larutan perak nitrat (AgNO3) 1mM direduksi menggunakan ekstrak kulit pisang kepok yang didiamka selama 15 menit pada ruang gelap. Karakterisasi menggunakan spektrofotometer UV-Vis dan Scanning Electron Microscope (SEM). Analisis terhadap stpektra UV-Vis menunjukan bahwa nanopartikel relatif stabil pada panjang gelombang 414.50-447.00 nm. Hasil dari karakterisasi SEM menunjukan nanopartikel perak yang disintesis menggunakan ekstrak kulit pisang kepok dengan perak nitrat (AgNO3) 1mM memiliki ukuran terkecil 46 nm dan yang terbesar mencapai 65 nm. Hasil penelitian ini menunjukan bahwa nanopartikel perak yang disintesis menggunakan ekstrak kulit pisang kepok memiliki kandungan fenolik dan aktivitas antiofotooksidasi lebih rendah dibandingkan dengan ekstrak kulit pisang kepok tanpa nanopartikel perak. ABSTRACTThis study aims to determine the antiphotooxidation activity of silver nanoparticles synthesized by kepok banana peel. Silver nitrate (AgNO3) 1mM solution was reduced using kepok banana peel extract which was stored for 15 minutes in a dark room. Characterization used UV-Vis spectrophotometer and Scanning Electron Microscope (SEM). Analysis of UV-Vis spectrum showed that nanoparticles were relatively stable at wavelength 414.50-447.00 nm. The results of SEM characterization showed that silver nanoparticles synthesized by 1mM kepok banana peel extract with silver nitrate (AgNO3) had the smallest size of 46 nm and the largest reached 65 nm. The results of this study showed that silver nanoparticles synthesized by kepok banana peel extracts had a lower phenolic content and antiophotooxidation activity compared to kepok banana peel extract without silver nanoparticles.
AKTIVITAS TABIR SURYA DARI FRAKSI FENOLIK BUAH SIRIH HUTAN (PIPER MINIATUM. BL) Budiarso, Leo Arifsandi; Suryanto, Edi; Sudewi, Sri
CHEMISTRY PROGRESS Vol 8, No 1 (2015)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.8.1.2015.9400

Abstract

This study was intended to determine the sunscreen activity from the phenolic fraction of betel fruits. Betel fruits powder extracted with maseration method with ethanol 80%. Furthermore betel fruits extract liquid successively fractioned using petroleum eter, etyl acetate, butanol, ethanol and aquades. The phytochemical analysis of faction determined using total phenolic, flavonoid and condensed tannin content. The sunscreens activity was evaluated in sun protection factor (SPF) of fraction with spectrophotometry UV-vis.The best fractions were characterized functional group using infra red spectrophometer. The result showed that the butanol fraction have the highest total phenolic and condensed tannin content and petroleum eter fraction possessed highest total flavonoid content. Butanol fraction has the highest antioxidant activity than the other fraction. Aquades fraction (16.14) showed highest sun protection factor (SPF) value followed by petroleum eter, ethyl acetate, butanol and ethanol. SPF velue were 8.07; 6.93; 6.70; 5.38 dan 5.29.Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dan potensi tabir surya dari fraksi buah sirih hutan. Serbuk buah sirih hutan diekstraksi dengan metode maserasi menggunakan pelarut etanol 80%. Ekstrak buah sirih hutan difraksinasi berturut turut dengan petroleum eter, etil asetat, butanol, etanol dan akuades. Analisis kandungan fitokimia fraksi-fraksi ditentukan berdasarkan total fenolik, flavonoid dan tannin terkondensasi. Aktivitas tabir surya pada fraksi-fraksi dievaluasi dengan analisis sun protection factor (SPF) menggunakan spektrofotometer UV-vis. Fraksi terbaik dikarakterisasi gugus fungsinya menggunakan spektrofotometer infra merah (FTIR). Hasil penelitian menunjukkan bahwa fraksi butanol memiliki kandungan total fenolik dan tanin terkondensasi tertinggi sedangkan kandungan flavonoid tertinggi terdapat pada fraksi butanol, petroleum eter dan etil asetat. Fraksi akuades memiliki aktivitas tabir surya tertinggi dibandingkan dengan fraksin diikuti fraksi petroleum eter, etil asetat, butanol dan etanol. Nilai SPF berturut-turut adalah 8,07; 6,93; 6,70; 5,38 dan 5,29.
EFEK PEMANASAN TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK EMPELUR BATANG SAGU BARUK (ARENGA MICROCARPHA B.) Landjang, Enrico Yoel; Momuat, Lidya; Suryanto, Edi
CHEMISTRY PROGRESS Vol 10, No 1 (2017)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.10.1.2017.27968

Abstract

The objective of this research was to acknowledge the effect of heating on the total phenolic compound of sago baruk trunk pith extract and the antioxidant activity of sago baruk trunk pith extract on preventing the oxidation of linoleic acid. Sago baruk trunk pith extract obtained from blending the sago baruk sample with the water solvent and was given a variety of temperature that are room temperature, 50 oC, 75 oC, and 100 oC. Total phenolic compound measured with Folin-ciocalteu method and the antioxidant activity of sago baruk trunk pith was tested to linoleic acid with Ferric Thiocyanate method to measure the peroxide inhibition percentage and Thiobarbituric Acid Reactive Substance method to measure the malonaldehyde inhibiton percentage. The result of total phenolic compound of sago baruk trunk pith extract when given the heating process with a total of 69,286-72,449 mg/kg and was higher compared to before given heating process. The best antioxidant activity of sago baruk trunk pith extract on preventing the peroxide and malonaldehyde was when given the 75 oC heating process with a value of 57,374% for peroxide inhibition and 69,527% for malonaldehyde inhibition. This research concluded that the sago baruk trunk pith extract has the best total phenolic compound and the antioxidant activity when given the 75 oC heating process.ABSTRAKPenelitian ini bertujuan untuk mempelajari efek pemanasan terhadap kandungan total fenolik dari ekstrak empulur sagu baruk serta aktivitas antioksidan ekstrak empulur sagu baruk dalam menghambat oksidasi asam linoleat. Ekstrak empulur sagu baruk diperoleh dari hasil blender sampel sagu baruk dengan pelarut air kemudian diberi perlakuan pada beberapa variasi suhu yaitu suhu kamar, 50 oC, 75 oC, dan 100 oC. Kandungan total fenolik diukur menggunakan metode Folin-ciocalteu dan aktivitas antioksidan dari ekstrak empulur sagu baruk diuji pada asam linoleat menggunakan metode Ferric Thiocyanate untuk menghitung persen penghambatan peroksida dan metode Thiobarbituric Acid Reactive Substance untuk mengukur persen penghambatan pembentukan malonaldehida. Hasil kandungan total fenolik ekstrak empulur sagu baruk ketika diberi perlakuan pemanasan dengan jumlah 69,286 ? 72,449 mg/kg dan lebih tinggi dibandingkan sebelum dipanaskan. Aktivitas antioksidan dari ekstrak empulur sagu baruk dalam menghambat peroksida dan malonaldehida yang terbaik ketika diberi perlakuan suhu 75 oC dengan nilai masing-masing 57,374% untuk penghambatan peroksida dan 69,527% untuk penghambatan malonaldehida. Penelitian ini menyimpulkan bahwa ekstrak empulur sagu baruk memiliki kandungan total fenolik serta aktivitas antioksidan terbaik ketika diberi perlakuan suhu 75 oC
Co-Authors Abigael, Reliana Adisti A. Rumayar, Adisti A. Aliwu, Indriwati Alvin Febryanto Ginting, Alvin Febryanto Amelia Walangitan Aneke Korua, Aneke Arizona, Rizki Audy Wuntu Bayu Etti Tri Adiyastiti Budi Prasetya Budi Sutedjo Dharma Oetomo, Budi Sutedjo Dharma Bulkaini Bulkaini, Bulkaini Chairil Anwar Chaleb P. Maanari Christine F. Mamuaja Christine Mamuaja Chusnul Hanim Colling, Erningsi Dewa G. Katja Dewa Gede Katja Djarkasi, G.S. Suhartati Djarkasi, Gregoria Djasibani, Hemy R. Dokri Gumolung Edam, Mariati Edam, Mariati Elisa Putri Tarigan, Elisa Putri Ering, Aprily A. P. Fajar Lestari Fetty Indriaty Frenly Wehantouw Gregoria S. S. Djarkasi Hardjono Sastrohamidjojo Harjono Sastrohamidjojo, Harjono Harris, M Ichsan Hasan Datu, Hasan Henry Aritonang Hindang S. Kaempe I Wayan Karda, I Wayan Inneke F.M Rumengan Iqra U. Kanopa Irma Momuat, Lidya Jamhari Jamhari Jeanette Manarisip Jeany Polii-Mandang Jemmy Abidjulu Jessy Paendong Johnly A Rorong Johnly A. Rorong Johnly Alfreds Rorong Judith Mandei Julius Pontoh Jumari Jumari, Jumari Kabalmay, Julista Alfa Kaemba, Almawaty Kaemba, Almawaty Laksmiwati, Diah Ayu Landeng, Patrisia Jaklin Landjang, Enrico Yoel Leo Arifsandi Budiarso, Leo Arifsandi Lexie Mamahit Lidya I. Momuat Lidya Irma Momuat Liemey Iviane Lumempouw Liemey Lumempouw Mamuaja, Christin Mastur Mastur Mawardi, Rizky H. Max R. J. Runtuwene Meiske Lumingkewas Meiske S. Sangi Mercy Taroreh Momuat, Lidya Mongan, Jemsi Nafly Comilo Tiven Nancy Kiay, Nancy NI LUH PUTU SRIYANI Nova . Olha Rantung, Olha Pangemanan, David Albert Pasanda, Imanuel Medy Pasanda, Immanuel M. Paulina V. Y. Yamlean Permadi, Edy Raganata, Tirza C. RETNO PENI SANCAYANINGSIH Riny Modaso Rompas, Veckie Frankie Rondonuwu, Samuel D. J. Rorong, Johnly Alfrets Rumengan, Inneke Rusman (Rusman) Rusman Rusman Ryan Vanly Saryana Sastrohamidjojo, H Sastrohamidjojo, H. Sembiring, Elia Sersermudy, Chesya Hana Shirley E. S. Kawengian Soeparno Soeparno Sri Febriani Hatam Sri Raharjo Sri Sudewi, Sri Sudiarso Sudiarso Sundari Sundari Tapa, Fitriyanti La Taroreh, Mercy R. I. Tranggono . Tranggono Tranggono Trina Tallei Umi Proboyekti Waworuntu, Mutiara G. Winny Swastike Yenny Tambunan Yuny Erwanto Zaenal Bachruddin