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REGENERASI BERBAGAI JENIS EKSPLAN NILAM KLON SIDIKALANG DAN APLIKASI AZOTOBACTER PADA TAHAP AKLIMATISASI Erni, Erni; Sobarna, Denny Sobardini; Nuraini, Anne; Mubarok, Syariful; Suryatmana, Pujawati; Sihombing, Yudhistari; Angel, Christine
Agrikultura Vol 27, No 2 (2016): Agustus, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (636.998 KB) | DOI: 10.24198/agrikultura.v27i2.9992

Abstract

AbstrakKondisi produktivitas nilam di Indonesia saat ini masih rendah, hal ini diduga disebabkan oleh rendahnya penggunaan benih unggul serta kendala ketersediaan benih yang tepat waktu, jumlah bibit yang seragam dan bebas penyakit, serta masih rendahnya mutu genetik tanaman. Sehingga perlu dilakukan upaya perbanyakan maupun peningkatan mutu genetik dengan aplikasi teknologiin vitro. Percobaan I: Pertumbuhan eksplan nilam pada komposisi sitokinin yang berbeda secara in Vitro (Rancangan Acak Lengkap pola faktorial), faktor pertama (jenis ekplan : mata tunas, pucuk, dan daun), sedangkan faktor kedua (0 mg/l sitokinin; 0,5 mg/l BAP; 1,0 mg/l BAP; 0,5 mg/l Zeatin; dan 1,0 mg/l Zeatin). Percobaan II: Multiplikasi stek mikro nilam secara in vitro (Rancangan Acak Lengkap). Percobaan III: Respon stekmikro terhadap inokulasi Azotobacter sp. pada tahap aklimatisasi (Rancangan Acak Kelompok) terdiri dari lima perlakuan (tanpa inokulasi, 1 ml, 2 ml dan 3 ml Azotobacter sp.). Hasil Percobaan I menunjukkan tidak terdapat interaksi antara jenis eksplan dengan jenis dan konsentrasi sitokinin. Penggunaan eksplan pucuk menghasilkan rata-rata jumlah tunas, pertambahan jumlah daun serta rata-rata bobot segar eksplan lebih baik serta penambahan 0,5 mg/l BAP pertambahan jumlah daun dan rata-rata bobot segar eksplan nilam lebih baik pada 8 MST. Percobaan II menunjukkan bahwa penggunaan 0,01 mg/l NAA dan 1 mg/l BAP merupakan perlakuan yang terbaik untuk multiplikasi tunas nilam pada peubah jumlah tunas dan jumlah daun. Percobaan III menunjukkan inokulasi Azotobacter sp. dengan dosis 3 ml memberikan rata-rata pertambahan jumlah daun, jumlah akar dan panjang tanaman relatif lebih baik.Kata kunci : Aklimatisasi, Azotobacter, Cytokinin, Eksplan, Nilam, Regenerasi
APLIKASI BAKTERI ENDOFITIK PENAMBAT N2 UNTUK MENINGKATKAN POPULASI BAKTERI ENDOFITIK DAN HASIL TANAMAN PADI SAWAH Setiawati, Mieke Rochimi; Arief, Dedeh H.; Suryatmana, Pujawati; Hudaya, Ridha
Agrikultura Vol 19, No 3 (2008): Desember, 2008
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.21 KB) | DOI: 10.24198/agrikultura.v19i3.1009

Abstract

Bakteri endofitik penambat nitrogen (N2) yang bersimbiosis dengan tanaman padi dapat digunakan sebagai pupuk hayati karena dapat memberikan nitrogen yang ditambatnya langsung pada tanaman. Penelitian pot ini bertujuan untuk mendapatkan konsentrasi dan teknik aplikasi pupuk hayati cair bakteri endofitik penambat N2 yang tepat dalam meningkatkan populasi bakteri endofitik penambat N2 dan  hasil tanaman padi sawah.  Percobaan dirancang dalam Rancangan Acak Kelompok yang menguji faktor konsentrasi pupuk hayati (25, 50, 75 dan 100 ml l-1 pupuk hayati) dan metode aplikasi pupuk (Perendaman, Penyemprotan dan kombinasinya) . Hasil percobaan memperlihatkan adanya peningkatan populasi bakteri endofitik penambat N2 di daun, batang, dan akar setelah aplikasi pupuk hayati baik melalui teknik perendaman, penyemprotan maupun kombinasinya. Aplikasi  100 ml l-1 pupuk hayati melalui semua teknik menghasilkan gabah kering panen 85,89 g tanaman-1, lebih besar  7,79 %  daripada penggunaan 50 ml l-1 pupuk hayati.
Aplication of Azolla pinnata and N-Fixing Endophytic Bacteria To Enhance Chemical, Plant Properties, and Dry Weight Corn Plant at Inceptisols Jatinangor Setiawati, Mieke Rochimi; Suryatmana, Pujawati; Machfud, Yuliati; Tridendra, Yori
Agrologia Vol 8, No 1 (2019)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v8i1.872

Abstract

The research to determine the effect of Azolla pinnata dry weight and nitrogen-fixing endophytic bacteria on the chemical properties of soil and plants and the growth of corn plants on Inseptisols from Jatinangor. This experiment was carried out in the greenhouse of the Faculty of Agriculture, Padjadjaran University, Jatinangor, West Java. The experiment design was Randomized Block Design (RDB) in factorial pattern consisted of two factors and three replications. The first factor was dosage of Azolla pinnata compost consisted of four levels (0 g pot-1, 12.5 g pot-1, 25 g pot-1, 37.5 g pot-1). The second factor was nitrogen-fixing endophytic bacteriaconsisted three levels: without nitrogen-fixing endophytic bacteria, just used nitrogen-fixing endophytic bacteria Acinetobacter sp., used nitrogen-fixing endophytic bacteria Pseudomonas sp. The results showed that there were interactions between Azolla pinnata and N2-fixing endophytic bacteria on N concentration and N uptake of corn plants. Applicaton Azolla pinnata compost with a dosage of 37.5 g pot-1 equivalent to 6 tons ha-1 can produce the highest dry weight of corn plants. Both nitrogen-fixing endophytic bacteria and azolla compost have not been able to increase the total N of Jatinangor Insepticols.Keywords: Azolla pinnata, nitrogen-fixing endophytic bacteria, Acinetobacter sp.,   Pseudomonas sp., corn plant
Pengaruh Aplikasi Pupuk Hayati, Vermikompos Dan Pupuk Anorganik Terhadap Kandungan N, Populasi Azotobacter sp. Dan Hasil Kedelai Edamame (Glycine max (L.) Merill) Pada Inceptisols Jatinangor. Setiawati, Mieke Rochimi; Sofyan, Emma Trinurani; Nurbaity, Anne; Suryatmana, Pujawati; Marihot, Gordon Pius
Agrologia Vol 6, No 1 (2017)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v6i1.174

Abstract

In Indonesia, Inceptisols is the largest soil order on which intensive agriculture is done. The fertility of this soil order is low, so that it is necessary to improve the soil fertility by using biofertilizers and organic matter. The objectives of this experiment were to determine the effect of a biofertilizer that contains microbe Azotobacter sp., Vermicompost and N, P, K Fertilizer on N content, the population of Azotobacter sp., and the yield of the Edamame soybeans at Inceptisols Jatinangor. A Field experiment was conducted by using Randomized Blocked Design consisted of 10 treatments and three replication. The combinations of these treatments consisted of: control (no treatment), the dose recommendation Urea 100 kg ha-1 + SP36 150 kg ha-1 + KCl 125 kg ha-1, 5 ton ha-1 vermicompost + ½ recommendation ha-1 N, P, K, 5 ton ha-1 vermicompost + 1 recommendation ha-1 N, P, K, 5 ton ha-1 vermicompost + 1½ recommendation ha-1 N, P, K, 10 ton ha-1 vermicompost + ½ recommendation ha-1 N, P, K, 10 ton ha-1 vermicompost + 1 recommendation ha-1 N, P, K, 10 ton ha-1 vermicompost + 1½ recommendation ha-1 N, P, K, 5 ton ha-1 vermicompost +  ½ recommendation ha-1 N, P, K + liquid biofertilizers 5 L ha-1, 10 ton ha-1 Vermicompost + ½ recommendation ha-1 N, P, K + liquid biofertilizers 5 L ha-1.  The experimental results showed that the combined treatment of liquid biofertilizer, Vermicompost and N, P, K fertilizer enhanced the population of Azotobacter sp. and weight of soybean but did not significantly influence N uptake.
KARAKTERISTIK PERTUMBUHAN DAN WAKTU GENERASI ISOLAT Azotobacter sp. DAN BAKTERI ENDOFITIK ASAL EKOSISTEM LAHAN SAWAH Setiawati, Mieke Rochimi; Suryatmana, Pujawati; Herdiyantoro, Diyan; Ilmiyati, Zahra
Jurnal Agroekoteknologi Vol 6, No 1 (2014)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.49 KB)

Abstract

Azotobacter sp. and N2-fixation endophytic bacteria are microbes that play a role in fixation the N2 from the free air and helps supply of N to plants and can make efficient use of N derived from inorganic fertilizer. N2-fixation microbes utilization in rice fields can reduce the cost of production of rice plants. By studying the characteristics of the two types of beneficial bacteria that is expected to be engineered to enhance the role of these bacteria contribute in supplying N to rice crops. The method used in this study is exploratory and observe the characteristics of growth and generation time of bacteria for 72 hours. The results of this study indicate that the bacterial isolates of Azotobacter sp-1 has the highest population in 48 hours with a total population of 1.58 x107 cfu ml-1 , whereas bacteria Azotobacter sp-2 has the highest population in 60 hours with a total population of 3.12 x106 cfu ml-1 . Both N2-fixation endophytic bacteria populations reached the highest peak in the 60th hour with a total population of N2-fixation endophytic bacteria-1 at 1.12 x 108 cfu ml-1 of N2-fixation endophytic bacteria-2 of 9.4 x 107 cfu ml-1 of both isolates endophytic bacteria showed a similar growth pattern. Azotobacter sp. isolates from rhizosfir of the rice plant have a difference generation time, while the two isolates of N2-fixation endophytic bacteria from tissue of rice plants having the same generation time. Isolates of Azotobacter sp.-1 had the fastest of generation time, capable of generating new cells every 158.66 minutes or every 2.64 hours.
POTENSI Azotobacter sp. ST.04 DAN KOMPOS Azolla pinata UNTUK MENINGKATKAN EFISIENSI BIODEGRADASI HIDROKARBON DALAM PROSES BIOREMEDIASI LIMBAH MINYAK BUMI Suryatmana, Pujawati; Setyawati, Mieke Rochimi
Jurnal Agroekoteknologi Vol 6, No 1 (2014)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.312 KB)

Abstract

The Petroleum hydrocarbon waste is one of Pollutant contributing which cause problem in environmental. Azotobacter is a rizobacteria type which is capable fixating dinitrogen. The result investigation showed that Azotobacter sp strain AV04 is capable producing many extra-cellulary biomolecule. That strain AV04 known is capable producing a biomolecule which acting as strong bioemulsifier. Azolla pinata is a hydro plant that can contributes of N, P dan K nutrition to the Soil. This plant has potentialy as a nutrition organic source. The research was focused on the role of the two agents, i.e. Azotobacter sp starin AV04 as the biosurfactan producing bacteria and Azolla pinata composted in the biodegradation hydrocarbon processes. Results showed that the Total Petroleum Hydrocarbon (TPH) efficiency in the 1st week and 5th week, which was in the treatment of 10% (w/w) of A. pinata and 2% (v/w) of Azotobacter sp starin AV04, was reaching in value 56.15 % and 98.03 % respectively. In the optimum treatment showed that the TPH removal efficiency consistently was significantly rising up as long as 5 week period of bioremediation. The residue compounds of Petroleum after one week period incubation on the optimum treatment were constituting hydrocarbon comprise of 26 species of hydrocarbon compound, while at the same time and in the control treatment founded the hydrocarbon compounds remain were still about 37 species of Hydrocarbon compound.
Populasi Bakteri Dan Jamur Serta Pertumbuhan Tanaman Teh (Camellia sinensis L.) Pada Dua Jenis Media Tanam Setelah Inokulasi Azotobacter Hindersah, Reginawanti; Adityo, Bagus; Suryatmana, Pujawati
Agrologia Vol 5, No 1 (2016)
Publisher : Pattimura University, Ambon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/a.v5i1.191

Abstract

Substituting a part of soil on tea Camellia Sinensis (L.) O. Kuntze) nurseries by using organic matter could minimize the remove fertile soil from nature. Biofertilizing are a way to improve the biological quality of soil and subsequently tea seedling quality. The aim of the research was to determine the effect of planting media composition and biofertilizer doses on total fungal and bacterial population on planting media; as well as tea seedlings growth. The experiment was conducted using a randomized block design consisting of 9 treatments and 4 replications, each replication consisted of three plants. The treatment consisted of soil without biofertilizer (control), soil + 5% biofertilizer, soil + 10% biofertilizer, cocopeat without biofertilizer, cocopeat + 5% biofertilizer, cocopeat + 10% biofertilizer,  filter cake without biofertilizer, filter cake + 5% biofertilizer, and filter cake + 10% biofertilizer. Biofertilizer used in this experiment were Azotobacter vinelandii and Azotobacter chroococcum that was mixed with growing media before planting. Result of the experiment showed that planting media affect microbial populations differently. The population of total bacteria, total fungi and Azotobacter sp. in the filter cake media with 10% biofertilizer was significantly higher than those of other growing media. However, Azotobacter biofertilizer had no effect on seedling growth of tea (Camellia Sinensis (L.) O. Kuntze) clones Gambung-7
Efek Sterilisasi dan Komposisi Media Produksi Inokulan Fungi Mikoriza Arbuskula terhadap Kolonisasi Akar, Panjang Akar dan Bobot Kering Akar Sorgum Dewi, Tarra Martiana; Nurbaity, Anne; Suryatmana, Pujawati; Sofyan, Emma Trinurani
Jurnal Agro Vol 4, No 1 (2017)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/1205

Abstract

Quality of biofertilizer such as arbuscular myorrhizal fungi could be improved through the selection of appropriate sterilization and composition of the medium. The objective of the experiment was to determine the effect of sterilization and carrier composition on root colonisation, root length and weight of sorghum root. A green house experiment was conducted in randomized block design. The factors were: 1. methods of sterilization (ie. control; autoclave; NaOCl 10%; Co-60 gamma irradiation), and 2. composition of the carrier of mycorrhiza (100% zeolite; 75% zeolite + 25% rice charcoal;  50% zeolite + 50% rice charcoal). Results showed that there was no interaction effect of sterilization and composition of carrier on root colonisation, root length and weight of sorghum root. However, different technique of sterilisation gave differences on root colonisation, root length and weight of sorghum root. Sterilization using NaOCl 10% was not recommended. Gamma Co-60 irradiation sterilization did not have difference with control and autoclave, however there was  tendency  that irradiation technique gave better results to all parameters measured.
Azotobacter population, soil nitrogen and groundnut growth in mercury-contaminated tailing inoculated with Azotobacter Hindersah, Reginawanti; Handyman, Zellya; Indriani, Febby Nur; Suryatmana, Pujawati; Nurlaeny, Nenny
Journal of Degraded and Mining Lands Management Vol 5, No 3 (2018)
Publisher : University of Brawjiaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (707.751 KB) | DOI: 10.15243/jdmlm.2018.053.1269

Abstract

Gold mine tailing in Buru island, Maluku Province, Indonesia contains high level of mercury but low in carbon and plant nutrients. Revegetation in such condition needs certain soil treatment which is suitable for plant growth. The objective of pot trials was to study the effect of indigenous Plant Growth Promoting Rhizobacteria Azotobacter and organic matter on bacterial survival as well as growth of groundnut grown in mine tailing. The experimental design was a Split Plot Design which tested three types of Azotobacter liquid inoculant and three soil total organic carbon (TOC) contents. Results showed that Azotobacter inoculation increased Azotobacter population in tailing at the end of vegetative growth of groundnut. Total nitrogen content in soil decreased when TOC level increased. However, nitrogen uptake and growth of groundnut did not change after Azotobacter inoculation or manure amendment. These experiments provided information that Azotobacter inoculation on groundnut maintain its proliferation in Hg-contaminated mine tailing.
Peningkatan produktivitas tanaman kedelai kultivar Anjasmoro asal benih terdeteriorasi dengan kompos Trichoderma dan bokashi Sumadi, Sumadi; Sobarna, Denny Sobardini; Suryatmana, Pujawati; Rachmadi, Meddy; Suminar, Erni
Kultivasi Vol 17, No 3 (2018)
Publisher : Fakultas Pertanian UNPAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.751 KB)

Abstract

Sari. Trichoderma spp merupakan golongan fungi yang bersifat antipatogen, khususnya layu kecambah, sedangkan bokashi merupakan kompos yang diperkaya dengan beberapa mikroba yang bermanfaat bagi pertumbuhan tanaman.Tulisan ini merupakan rangkuman penelitian pot menggunakan kompos Trichoderma spp sebagai pelapis benih terdeteriorasi disertai bokashi kotoran hewan yang dilakukan pada tanah lahan kering dan tanah sawah pada tahun 2014 dan 2017. Percobaan pertama membandingkan efektivitas beberapa agen hayati sebagai pelapis benih pengaruhnya terhadap vigor benih dan hasil tanaman. Hasil percobaan menunjukkan penggunaan kompos Trichoderma hasilnya lebih baik dibandingkan agen hayati lainnya. Hasil percobaan selanjutnya menunjukkan  bahwa pelapisan benih dengan 1 sampai 3 g  kompos Trichoderma spp per 100 butir benih pengaruhnya tidak nyata dibandingkan dengan penggunaan pelapis benih berupa pestisida, baik terhadap vigor  maupun hasil biji per tanaman. Pemberian bokashi sebaliknya secara nyata mampu meningkatkan hasil biji per tanaman . Pemberian bokashi 15 t ha-1menghasilkan biji seberat 19.83 g per tanaman  atau setara dengan 2,379 t ha-1 atau 27,3 % lebih tinggi dari kontrol.Kata kunci : Benih terdeteriorasi, pelapisan benih, Trichoderma, Bokashi Abstract. Trichoderma spp is a group of antipatogenic fungi, especially seedling wilted, while bokashi is a compost enriched with some microbes that are beneficial to plant growth. This paper is a compilation of pot experiment using Trichoderma compost for coating deteriorated seed and accompanied by bokashi on dry land soil and paddy soil in 2014 and 2017 respectively. The first experiment compares the effectiveness of several biological agents as a seed coating of its effect on seed vigor and yield. The results of first experiment showed that the use of 2 g Trichoderma compost 100 seeds-1 was better than other biological agents. However between 1.2 and 3 g of Trichoderma compost per 100 seeds was not significant effect on seed vigor and yield. The results of experiment showed that the coating of seeds with 1 – 3 g of Trichoderma spp compost 100 compared with pesticide seed coatings. Increased of yield only affected bokashi application. Application of bokashi 15 t ha-1 abble to produce 19.83 g seed per plant or equivalent to 2.379 t ha-1 or 27.3% higher than the control.Key words: deteriorated seeds, seed coating, Trichoderma, bokashi