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PENETAPAN KADAR FENOLIK TOTAL EKSTRAK METANOL KELOPAK BUNGA ROSELLA MERAH (Hibiscus sabdariffa Linn) DENGAN VARIASI TEMPAT TUMBUH SECARA SPEKTROFOTOMETRI Alfian, Riza; Susanti, Hari
PHARMACIANA Vol 2, No 1: Mei 2012
Publisher : PHARMACIANA

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Abstract

Penelitian ini bertujuan untuk menetapkan kadar fenolik total pada kelopak bunga rosella merah (Hibiscus sabdariffa Linn) dengan variasi tempat tumbuh. Kelopak bunga Rosella merah diambil dari daerah Glagah, Kediri dan Samigaluh. Senyawa fenolik dalam kelopak bunga rosella diekstraksi menggunakan metode maserasi dengan pelarut metanol. Kadar fenolik total ditetapkan menggunakan metode Spektrofotometri visibel dengan pereaksi Folin Ciocalteau. Prinsip dari metode ini adalah terbentuknya senyawa kompleks berwarna biru dari fosfomolibdatfosfotungstat yang direduksi senyawa fenolik dalam suasana basa yang dapat diukur secara spektrofotometri. Sebagai pembanding digunakan asam galat. Kadar fenolik total pada kelopak bunga Rosella merah Glagah, Kediri dan Samigaluh berturut-turut yaitu 1,40 g GAE/100 g ekstrak, SD 0,06 dengan n=12; 1,41 g GAE/100 g ekstrak, SD 0,07 dengan n=12 dan 2,12 g GAE/100 g ekstrak, SD 0,05 dengan n=15. Dapat disimpulkan bahwa tempat tumbuh berpengaruh terhadap kadar fenolik total dalam ekstrak metanol kelopak bunga Rosella merah.
PENGHAMBATAN AKTIVITAS XANTHINE OXIDASE OLEH EKSTRAK ETANOL AKAR SAMBILOTO (Andrographis paniculata,Ness) SECARA IN VITRO Septianingsih, Ulfah; Susanti, Hari; Widyaningsih, Wahyu
PHARMACIANA Vol 2, No 2: November 2012
Publisher : PHARMACIANA

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Abstract

flavonoid yang digunakan masyarakat untuk pengobatan tradisional. Dari penelitian terdahulu dilaporkan bahwa senyawa flavonoid dapat berpotensi menurunkan kadar asam urat darah dengan cara menghambat aktivitas xanthine oxidase. Oleh karena adanya flavonoid yang terkandung dalam akar Sambiloto maka dilakukan penelitian apakah ekstrak etanol akar Sambiloto dapat menghambat aktivitas xanthine oxidase. Sebagai pembanding digunakan Allopurinol. Ekstrak etanol dibuat dari serbuk akar Sambiloto diekstraksi dengan etanol menggunakan metode penyarian dengan alat Soxhlet, sebelum penyarian dilakukan pengawalemakan menggunakan petroleum eter. Penghambatan aktivitas xanthine oxidase oleh ekstrak etanol akar Sambiloto ditentukan melalui penurunan produksi asam urat yang dimonitor dengan spektrofotometer pada 295 nm dengan xanthine sebagai substrat. Nilai kecepatan yang diperoleh selanjutnya digunakan untuk menghitung nilai aktivitas. Kemudian ditentukan konsentrasi ekstrak etanol yang mampu menghambat aktivitas xanthine oxidase sebesar 50% (IC50). Hasil dianalisis secara kuantitatif, dengan menggunakan uji Kruskal Wallis dengan taraf kepercayaan 95%, kemudian dilanjutkan dengan uji Mann Withney. Flavonoid dalam ekstrak etanol dipisah dengan cara kromatografi kertas dan perubahan bercak ditentukan dengan UV 366 dengan dan tanpa pemberian uap amoniak. Hasil penelitian menunjukkan bahwa ekstrak etanol akar Sambiloto menghambat aktivitas Xanthine Oxidase dengan IC50 16,82 μg/ml sedangkan IC50 Allopurinol adalah 4,29 μg/ml. Ekstrak etanol akar Sambiloto diduga mengandung flavonoid golongan flavon atau flavonol.
UJI EFEK ANTIHIPERGLIKEMIK EKSTRAK ETANOL DAUN KACAPIRING (Gardenia augusta, Merr) PADA TIKUS PUTIH JANTAN GALUR WISTAR Baroroh, Faridah; Aznam, Nurfina; Susanti, Hari
PHARMACIANA Vol 1, No 1: Mei 2011
Publisher : PHARMACIANA

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Abstract

Daun kacapiring (Gardenia augusta, Merr) sering digunakan secara tradisional untuk pengobatan diabetes mellitus. Penelitian ini dilakukan untuk mengetahui apakah ekstrak etanol daun kacapiring dapat menurunkan kadar glukosa darah pada tikus putih jantan galur Wistar dan seberapa besar efek antihiperglikemiknya jika dibandingkan dengan obat antidiabetes glibenklamid. Penelitian ini menggunakan metode uji toleransi glukosa oral dengan pembebanan glukosa dosis 4,5 g/kgBB. Hewan uji yang digunakan tikus putih jantan galur Wistar umur 2-3 bulan, berat badan 180-250 gram, sebanyak 24 ekor yang dibagi menjadi 4 kelompok masing-masing kelompok terdiri dari 6 ekor tikus. Kelompok I sebagai kelompok kontrol negatif diberi CMC-Na 1%, kelompok II sebagai kelompok kontrol positif diberi glibenklamid dosis 1,35 mg/kgBB, kelompok III dan kelompok IV diberi ekstrak etanol daun kacapiring masing-masing dosis 500 mg/kgBB dan 250 mg/kgBB. Glibenklamid dan ekstrak diberikan secara peroral 60 menit sebelum pemberian glukosa. Pengambilan darah melalui sinus orbitalis secara keseluruhan dilakukan pada menit ke-(-90), (-60), 0, 30, 60, 120, 180, 240, dan 300. Kadar glukosa darah diukur dengan metode enzimatik dengan pereaksi GOD PAP (Glucose Oxidase Phenol 4-Aminoantipirin) yang menghasilkan larutan merah dan absorbansi dibaca dengan spektrofotometer UV-Vis pada panjang gelombang 500 nm. Hasil penelitian menunjukkan bahwa ekstrak etanol daun kacapiring dosis 500 mg/kgBB dan 250 mg/kgBB dapat berefek menurunkan kadar glukosa darah. Ekstrak etanol daun kacapiring dosis 500 mg/kgBB dan 250 mg/kg dapat menurunkan kadar glukosa darah sebesar 58,97% dan 80,60% disbanding glibenklamid dosis 1,35 mg/kgBB yang dapat menurunkan kadar glukosa darah sebesar 73,93%.
PENETAPAN KADAR ß-KAROTEN PADA BEBERAPA JENIS CABE (Genus Capsicum) DENGAN METODE SPEKTROFOTOMETRI TAMPAK Octaviani, Tri; Guntarti, Any; Susanti, Hari
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.451 KB) | DOI: 10.12928/pharmaciana.v4i2.1566

Abstract

Beta carotene is provitamin essential role for vitamin A formation. Most of vitamin Asources are ß-carotene. In the body, ß-carotene will be converted into vitamin A. Chilies areconsidered as one of the essential food. Chillies are known as very good sources of ß-carotene.Therefore, it is important to conduct a research in order to observe the content of ß-carotene inthe chillies using visible spectrophotometry. Fresh chillies were extracted with a mixture ofhexane:acetone:ethanol (2:1:1) v/v using a maceration method. After the extraction process wascomplete, aquabidest was added. The extraction result was non-polar phase. It was separatedand evaporated. The residue was further subjected to qualitative and quantitative analysis102 Pharmaҫiana, Vol. 4, No. 2, 2014: 101-109obtained. Qualitative analysis by using Carr-Price method. Determination of ß-carotene wasconducted using visible spectrophotometry method at 452,4 nm. The qualitative test resultsshowed that the Capsicum annuum L. Var. abreviatum Fingerhuth, Capsicum annuum L. Var.Longum sendt) and Capsicum frutescens L. contain ß-carotene. From the research, it wasobtained that the average levels of ß-carotene on the Capsicum annuum L. Var. abreviatumFingerhuth was (10,54±0,07) mg/100g, Capsicum annuum L. Var. Longum sendt was(5,57±0,13) mg/100g and Capsicum frutescens L. was about (0,36±0,01) mg/100g. Statisticalanalysis using LSD test of ß-carotene levels in the Capsicum annuum L. Var. abreviatumFingerhuth, Capsicum annuum L. Var. Longum sendt) and Capsicum frutescens L. weresignificantly different at P of 0.05.
EFEK ANTIANGIOGENESI EKSTRAK ETANOL GANGGANG HIJAU (Spirogyra sp.) BERDASARKAN EKSPRESI COX-2 PADA SEL T47D Widyaningsih, Wahyu; Salamah, Nina; Susanti, Hari; Fitriani, Dwi
Majalah Obat Tradisional Vol 19, No 3 (2014)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (974.752 KB) | DOI: 10.14499/mot-TradMedJ19iss3pp127-132

Abstract

Kanker adalah sekelompok penyakit yang timbul apabila sebuah sel atau sekelompok sel lepas dari kontrol yang mengatur pertumbuhan. Ganggang hijau (Spirogyra sp.) merupakan salah satu tanaman obat yang digunakan sebagai obat tradisional untuk pengobatan kanker. Ganggang hijau (Spirogyra sp.) mempunyai kandungan zat aktif berupa melatonin dimana melatonin merupakan senyawa yang sudah diteliti para peneliti dunia sebagai obat antikanker dan antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol ganggang hijau (Spirogyra sp.) terhadap ekspresi COX-2 pada sel T47D. Ganggang hijau diekstraksi dengan menggunakan alat Soxhlet dengan pelarut etanol 96%. Penelitian ini menggunakan 3 kelompok yaitu kontrol sel, pemberian ekstrak etanol ganggang hijau konsentrasi 247,668 μg/ml dan 123,834 μg/ml. Untuk memastikan adanya ekspresi COX-2 dilakukan uji imunositokimia secara tidak langsung. Pengamatan dilakukan menggunakan mikroskop cahaya untuk melihat dan menghitung persen ekspresi COX-2. Hasil yang didapatkan menunjukkan terdapat perbedaan yang bermakna penurunan ekspresi COX-2 pada tiap kelompok.. Jadi dapat disimpulkan bahwa ekstrak etanol ganggang hijau (Spirogyra sp.) mampu menurunkan ekspresi COX-2 pada sel T47D.
Subchronic Toxicity of Green Algae (Spyrogyra sp.) Ethanolic Extract on Hematologic Parameters Salamah, Nina; Widyaningsih, Wahyu; Susanti, Hari; Devi, Anggita; Sejati, Anita Wening; Putri, Zahra Alya
International Journal of Public Health Science (IJPHS) Vol 5, No 4: December 2016
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v5i4.5962

Abstract

Green Algae, an organism with active substance such as phytomelatonin, has potential to be developed as Indonesian traditional medicine. As the long term addition of Green Algae ethanol extract (Ekstrak etanol ganggang hijau, EEGH) influences the hematology system, in this paper, the safety test was done to ensure the safety of its use through subchronic toxicity test of EEGH on the hematology parameters of Wistar rats. The test group consisted of three groups treated with EEGH 100 mg/kg, 200 mg/kg, and 400 mg/kg, while the control group was given by 0.5% CMC-Na, with 8 rats each respectively. By using blood samples taken from orbital sinus on the 29th day, common hematologic parameters (erythrocytes, leukocytes, and hemoglobin level), the parameters of hemostasis (platelets, pT, aPTT, BT) and immune parameters (Differential Leukocytes Counts include neutrophils segment, lymphocytes, monocytes, and eosinophils) were finally observed and showed that the 28 days-addition of EEGH increase the hematological parameters of Wistar rats.
PENETAPAN KADAR ß-KAROTEN PADA BEBERAPA JENIS CABE (Genus Capsicum) DENGAN METODE SPEKTROFOTOMETRI TAMPAK Octaviani, Tri; Guntarti, Any; Susanti, Hari
Pharmaciana Vol 4, No 2 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.451 KB) | DOI: 10.12928/pharmaciana.v4i2.1566

Abstract

Beta carotene is provitamin essential role for vitamin A formation. Most of vitamin Asources are ß-carotene. In the body, ß-carotene will be converted into vitamin A. Chilies areconsidered as one of the essential food. Chillies are known as very good sources of ß-carotene.Therefore, it is important to conduct a research in order to observe the content of ß-carotene inthe chillies using visible spectrophotometry. Fresh chillies were extracted with a mixture ofhexane:acetone:ethanol (2:1:1) v/v using a maceration method. After the extraction process wascomplete, aquabidest was added. The extraction result was non-polar phase. It was separatedand evaporated. The residue was further subjected to qualitative and quantitative analysis102 Pharmaҫiana, Vol. 4, No. 2, 2014: 101-109obtained. Qualitative analysis by using Carr-Price method. Determination of ß-carotene wasconducted using visible spectrophotometry method at 452,4 nm. The qualitative test resultsshowed that the Capsicum annuum L. Var. abreviatum Fingerhuth, Capsicum annuum L. Var.Longum sendt) and Capsicum frutescens L. contain ß-carotene. From the research, it wasobtained that the average levels of ß-carotene on the Capsicum annuum L. Var. abreviatumFingerhuth was (10,54±0,07) mg/100g, Capsicum annuum L. Var. Longum sendt was(5,57±0,13) mg/100g and Capsicum frutescens L. was about (0,36±0,01) mg/100g. Statisticalanalysis using LSD test of ß-carotene levels in the Capsicum annuum L. Var. abreviatumFingerhuth, Capsicum annuum L. Var. Longum sendt) and Capsicum frutescens L. weresignificantly different at P of 0.05.
STABILITAS EPIGALOKATEKIN GALAT DALAM KRIM EKSTRAK TEH HIJAU DENGAN VARIASI KONSENTRASI ANTIOKSIDAN VITAMIN C 1% DAN VITAMIN E 1% Sugihartini, Nining; Susanti, Hari; Zaenab, Zaenab; Hanifah, Hana; Marlina, Siti Ayu
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 13, No 2 (2016)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.558 KB) | DOI: 10.24071/jpsc.132186

Abstract

Epigallocatechingallate (EGCG) in green tea extract has activity as an anti-inflammatory agent. On the other hand the stability of EGCG is poor because of the oxidation. The aim of this study was to determine the influence of Vitamine C and Vitamine E in formulation of green tea extract cream to the stabiliy of EGCG. The green tea extract was obtained from the extraction process by infundation followed by fractination with ethyl acetate as the solvent. The three formulas were compiled in similar composition with the concentration of vitamine C 1% (FI), Vitamine E 1% (FII) and there was no Vitamine C and Vitamine E (FIII) as a control. The EGCG level was determinated by TLC-densitometry methode. The stability parameter was determinated by calculated of the Q10 of each formula. The result of this study showed that the parameter of t90 of EGCG with Vitamine C 1%, Vitamine E 1% and control addition were 0.0108 hours, 0.0087 hours, 0.0084 hours, respectively. Stability of EGCG in green tea leaf extract cream with addition of the vitamin C 1% was higher than it stability with the addition of vitamin E 1%. The concentration of Vitamin C 1% was the optimum concentration as antioxidant in formulation of green tea extract cream.  
PERBANDINGAN METODE SOMOGYI-NELSON DAN ANTHRONE-SULFAT PADA PENETAPAN KADAR GULA PEREDUKSI DALAM UMBI CILEMBU (Ipomea batatas L.) Al-kayyis, Hasanul Kiyan; Susanti, Hari
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 13, No 2 (2016)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.425 KB) | DOI: 10.24071/jpsc.132191

Abstract

Sweet potato cultivar Cilembu, kind of ‘rancing’ from Cilembu Village, Sumedang, West Java has really sweet taste due to the high content of reducing sugar. The amount of reducing sugar needs to be proven scientifically. The study aimed to compare Somogyi-Nelson method and Anthrone-Sulfate method in order to determine reducing sugar level in Cilembu sweet potato. Cilembu sweet potato must be stored in freezer at 15°C for 2 weeks before used as the sample. Analytical method validation was performed for the two methods. The determination results between two methods were compared. The amount of reduction sugar (glucose) from Cilembu sweet potato determined by Somogyi-Nelson method was 3.42%, the value of %recovery was 99.8%, LOD = 0.00167 (mg/mL), LOQ = 0.00557 (mg/mL), and value of RSD was 3.7%. While the average amount of reduction sugar determined by Anthrone-Sulfate method was 14.9%, the value of %recovery was 74.7%, LOD = 0.0198 (mg/mL), LOQ = 0.066 (mg/mL), and value of RSD = 8.6%. The Somogyi-Nelson method was more recommended for the analysis of the reducing sugar in Cilembu sweet potato compared with Anthrone-Sulfate method.
KANDUNGAN BETA KAROTEN DAN AKTIVITAS PENANGKAPAN RADIKAL BEBAS TERHADAP DPPH (1,1-difenil 2-pikrilhidrazil) EKSTRAK BUAH BLEWAH (Cucumis melo var. Cantalupensis L) SECARA SPEKTROFOTOMETRI UV-VISIBEL Kusbandari, Aprilia; Susanti, Hari
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 14, No 1 (2017)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (523.041 KB) | DOI: 10.24071/jpsc.141562

Abstract

Cantaloupe (Cucumis melo var. Cantalupensis L.), a fruit containing some compounds, have activity to protect human body from free radical. The beta carotene (provitamin A) was recognized as antioxidant compound. Antioxidants could protect the body from cardiovascular damage caused by free radical.  The aims of this study was to determine the levels of beta carotene and antioxidant activity (IC50) from Cucumis melo var. cantalupensis L. extract using DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Cantaloupe was extracted using n-hexan-acetone-methanol ratio (2: 1: 1). Identification of beta carotene was performed using 25% antimony trichloride reagent followed by TLC and UV-Vis spectrum test compared with beta carotene as standard. The content of beta carotene and free radical scavenging activity was measured spectrophotometrically at 425 nm. The beta carotene level of cantaloupe was 3.171 ± 0.150 %. The antioxidant activity, presented as IC50 value of cantaloupe extract, was 12.137±0.44 µg/mL and the antioxidant activity of standard beta carotene was 2.15 ± 0.172 µg/mL. The cantaloupe extract contained beta carotene compunds but the antioxidant activity of extract was lower than beta carotene standard.