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KANDUNGAN LIPID BEBERAPA JENIS SIANOBAKTERIA LAUT SEBAGAI BAHAN SUMBER PENGHASIL BIODIESEL Sobari, Rifana; Susanto, Antonius Budi; Susilaningsih, Dwi; Rahma, Delicia Yunita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v2i1.2063

Abstract

Microalgae are an organism that contains of chlorophyll, so it can make the process of photosynthesis. The process of photosynthesis is the content of lipid producing microalgae as a potential feedstock biodiesel producer. Microalgae have the ability to generate huge natural oils (lipids). The aim of this study is to investigate the characteristic of morfologi and growth of microalgae marine cyanobacteria of Indonesian water that have the most lipid content, so it can be used as raw material for biodiesel. The research was carried out in September 2011 - February 2012. The implementation of the research carried out at the Laboratory of Bioprocess, Biotechnology Research Center Indonesian Institute of Sciences (LIPI) Cibinong, Bogor. Result microalga identification done using Nikon SE light microskop with identification book whareas lipid content ekstraction microalga done with Bligh and Dyer method (1959). The results showed that the microalgae sianobacteria had base of morfologi characteristic is the type of Oscillatoria sp.1, Oscillatoria sp.2, Nostoc sp., Synecococcus sp. and Lyngbya sp.. The highges growth OD marine cyanobacteria is type of Nostoc sp. and the lowest growth is type of Oscillatoria sp.2. Lipid content on Oscillatoria sp.1 4,2 mg/25 ml, Oscillatoria sp.2 3,7 mg/ 25 ml, Nostoc sp. 3,3 mg/25 ml, Synecococcus sp. 4 mg/25 ml and Lyngbya sp. 7,1 mg/25 ml.
PENGARUH SUBSTITUSI LIMBAH CAIR TAHU UNTUK MENSTIMULASI PEMBENTUKAN LIPIDA PADA CHLORELLA SP. Harahap, Puspita Sari; Susanto, Antonius Budi; Susilaningsih, Dwi; Rahma, Delicia Yunita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v2i1.2070

Abstract

Chlorella sp. is one of microalgae which has potential lipid content. Chlorella sp. has a rapid growth rate and also easy to maintain. Therefore, chlorella has potency as source in biodiesel production.This aim of study is to determine substitution of carbon source as regular medium with tofu liquid waste to enhanced lipid production of Chlorella sp. This research was conducted by laboratory experimental method using Completely Randomized Design (CRD) with six treatments (three replicates) and one control. Material in this research is Chlorella sp. that obtained from Puslit Bioteknologi- Bioproses division, Lembaga Ilmu Pengetahuan Indonesia, Cibinong. Research?s data is analyzed using analysis variant (for the normal and homogenous data) or using non-parametric (for the abnormal and in homogenous data). The result of this study indicates treatment which has higher lipid is Chlorella sp.?s cultivation with tofu liquid waste addition 15 % in day 42nd (0,5160 g/ L), and treatment with tofu liquid waste addition 20 % in day 45th has the highest oil content (0,4040 g/ L).Culture of Chlorella sp. with tofu liquid waste as medium resulted decrese lipid production.
OBSERVATION, ISOLATION AND CHARACTERIZATION OF MICROALGAL RED TIDE AGENT DINOFLAGELLATES PROROCENTRUM SP. (PENGAMATAN, ISOLASI DAN KARAKTERISASI MIKROALGA RED TIDE DARI DINOFLAGELLATA PROROCENTRUM SP) Susilaningsih, Dwi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 19, No 3 (2014): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.656 KB) | DOI: 10.14710/ik.ijms.19.3.149-158

Abstract

Spesies Dinophyte mempunyai habitat dari kutub, perairan tropis, tetapi semakin berlimpah di perairan tropis atau hangat. Dinophyte diduga sebagai penyebab terjadinya "red tide" sehingga nampak berwarna kuning kemerahan di laut ketikan malam hari disebabkan aktivitas bioluminescence.Penelitian ini menggunakan Dinophyte yang diisolasi dari pantai dan sampel air yang diperoleh dari di Prefektur Iwate, Jepang. Tujuan dari penelitian ini adalah untuk mengetahui taksonomi yang menyebabkan blooming. Spesies ini memiliki karakter yang spesifik bernbentuk oval (panjang 20-30 ?m dan lebar 1-20 ?m), kloroplas berwarna kuning, nukleus yang besar, dua flagel yang berbeda, yang salah satunya disebut flagellum transfer, tidak memiliki selaput tengah yang, ornament sel yang indah "theca"dengan tulang belakang. Berdasarkan hasil squensing pada 18 S rDNA, Dinophyte mempunyai kesamaan dengan strain Prorocentrum MBIC11147 (100%), Di masa yang akan datang penelitian Procentrum sp. bisa menggunakan sebagai model squensing, perilaku pasang mikroalga. Kata kunci: alga, Dinophyte, karakterisasi, isolation, Prorocentrum, red tide Dinophyte species inhabit from polar, temperate to tropical waters, but tend to be more abundant in tropical or warm waters. The Dinophytes is suspected as one of the genera causing red tide in the sea with their yellow-redish colour that make the sea glows in the night because of their bioluminescence activity. In this work, the Dinophyte was isolated from offshore, and water sample collected in Iwate Prefecture, Japan. Purposes of the studies were for understanding the taxonomic features in particular of the dinophytes that usually occur in blooming areas. The species has specific characters, such as oval shape ( 20-30 ?m long and 1-20 ?m wide), yellow chloroplast, large nucleus, possesses two different flagellas which one of them is specific called transfer flagellum, no middle furrow and beautiful ornament cell covering (theca) with spine. Based on the partial sequencing of the 18 S rDNA, the Dinophyte is precisely same as the reference strain Prorocentrum MBIC11147 (100%), which was determined as Prorocentrum sp. In the future, this study could be uses as model of sequel behavior of the microalgal red tide. Keywords: algae, Dinophyte, characterization, isolation, Prorocentrum, red tide
OUTDOOR CLOSED SYSTEM OF ALGAL MASS CULTURE : IN SIGHT OF COMPARISON ON VERTICAL AND HORIZONTAL PHOTOBIOREACTOR FOR CULTIVATING THE SPIRULINA SP. Farida, Hilda; Harahap, Puspita Sari; Sobari, Rifana; Gunawan, Rudyanto; Rahman, Delicia Yunita; Susilaningsih, Dwi
Reaktor Volume 19 No. 2 June 2019
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (505.913 KB) | DOI: 10.14710/reaktor.19.2.54-61

Abstract

Spirulina are multiceluller and filamentous blue-green algae that has gained considerable popularity in the health food industry and increasingly as a protein and vitamin supplement to aquaculture diets. The challenge for economically and fulfill the requirement for food and medical purposes has create many ways for mass-growth production, that possibly cultivated in the open-system (such as a raceway pond) or closed-system photobioreactors (such as tubular, bubble-column, airlift, flat-panel, and vertical). the cultivation of teh Spirulina on the vertical and horizontal photobioreactor has been studied. The photobioreactor, namely BJVP and BJHP, has a design to be less energy consumption using the air bubbling or circular paddle. The observation was conducted in a whole year with parameters of rainfall, temperature, light intensity, pH, and salinity. Result  showed that cultivation of Spirulina on the vertical photobioreactor growth faster than teh horizontal photobioreactor systems and the yield of biomass was about 0.94 gDW/L. Average of temperature ranges of BJHP were 31.0C-35.5C, salinities were 35 per mil level, pH were 8.55-10.86, and light intensity were 427-2001 umol photon s-1m-2. Whereas the BJVP has averages temperature range of 31.4C-33.9C, salinity 33-35 per mil level, pH 8.46-10.75, and light intensity 532-2062 umol photon s-1m-2. The proximate analyses of biomass from BJVP cultivation shows has tendency higher protein content compared to BJHP. The optimization of both reactors has continuing evaluated in order to get the optimum parameters required for economically Spirulina cultivation systems.Keywords: Spirulina, BJVP, BJHP, outdoor mass cultivating system, photobioreactor.
Substrates Preparation from Woody Tropical Waste Biomass for Biohydrogen Production Susilaningsih, Dwi; Harwati, Theresia; Anam, Khairul; Yopi, Yopi
Makara Journal of Technology Vol 12, No 1 (2008)
Publisher : Directorate of Research and Community Services, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.658 KB) | DOI: 10.7454/mst.v12i1.140

Abstract

Addressing to the global warming problem, energy crisis and pollution, hydrogen production by micro-organisms using biotechnological approach should be considered, since it fulfils the recent society requirement to safely produce, renewable and environmental friendly energy. Hydrogen is one of the most promising green energy sources, because it is easily converted to electricity and cleanly combustible. There are three types of micro-organisms for hydrogen production, the first is cyanobacteria through the photosynthesis process, the second is anaerobic bacteria, which use organic substances as electron donor and energy and convert them to hydrogen, the third is photosynthetic bacteria, somewhat between photosynthetic and anaerobic bacteria, which are capable of converting the organic substances to hydrogen at a fairly high rate. We propose to use the abundant waste biomasses in Indonesia for hydrogen production by the microbial system. Our focus research is the production of hydrogen from waste biomasses by two-stage fermentation systems, which combine the conversion process of monomer biomasses to lactic acid by Lactobacillus sp. and the conversion process of lactic acid to hydrogen by photosynthetic bacteria. In this research, two kind substrates preparation were apply for woody waste biomass such as chemical hydrolysis and biological methods with several treatments. The results of the substrate preparation state showed that hydrolyses process of biomasses using strong acid are yielded total sugar about 70-90% of previous original content. Moreover, hydrolyses process using weak/diluted acid are yielded total sugar about 4-30% of original sugar. Furthermore, the biological treatments of degradation of woody waste biomasses are yielded total sugar about 0-10% (by single culture) and 10-50% (by consortium). Those hydrolysates substrates will use for fermentation two stages of lactate fermentation and conversion by photosynthetic bacteria in order to produce hydrogen gas.
Identification of Bioactive Compound from Microalga BTM 11 as Hepatitis C Virus RNA Helicase Inhibitor Mustopa, Apon Zaenal; Umami, Rifqiyah Nur; Putri, Prabawati Hyunita; Susilaningsih, Dwi; Farida, Hilda
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1133.514 KB) | DOI: 10.14203/jbi.v11i2.2198

Abstract

ABSTRACTHepatitis C virus (HCV) is the major causative agent of chronic liver disease. Recently, the inhibition of NS3 RNA helicase/ATPase activity is being explored as the specifically targeted antiviral therapy (STAT) against HCV infection. This study was aimed to elucidate potential candidates for anti-HCV therapy derived from Indonesian indigenous microalgae. The microalga designated as BTM 11 was isolated and cultured. Methanol extract of BTM 11 was screened as the opponent of purified HCV NS3 RNA helicase enzyme through colorimetric ATPase assay. Screening of chemical compound and fractionation by using gel filtration chromatography with eluent of methanol : chloroform (1:99) were conducted for identification and isolation of the bioactive compounds. The third fraction of fractionated sample showed a relatively strong ATPase inhibitory effect (81.23 ± 2.25 %) compared to the negative control. Further analysis of third fraction using thin layer chromatography (TLC) with eluent of chloroform : methanol (9:2) gave two spots with the Rf value of 0.8 and 0.37, respectively. In addition, high performance liquid chromatography (HPLC) analysis showed absorption peak with the highest abundance at the retention time of 12.483 and 16.617 minutes which absorbed at 266 and 230 nm wavelenght, respectively. According to those analyses, this study suggests that bioactive compounds derived from BTM 11 were classified as the groups of flavonoids and feasible as potential candidates for anti-HCV therapy through the inhibitory effect of NS3 RNA helicase/ATPase activity. Keywords: Hepatitis C Virus, NS3 RNA helicase, ATPase, Microalga, Flavonoids 
BIODIVERSITY OF HYDROCARBON-PRODUCING MICROALGAE FROM OIL CONTAMINATED IN COASTAL ZONE OF BATAM ISLAND Susilaningsih, Dwi; Siburian, Marlinang Diarta; Murniasih, Tutik
Marine Research in Indonesia Vol 33, No 2 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5544.715 KB) | DOI: 10.14203/mri.v33i2.484

Abstract

Addressing to the environmental and energy crises in Indonesia, the new alternative of green-economic-energy sources is required. In this study marine microalgae were isolated from oil-polluted coastal zone in Batam Island for alternative energy sources. The research was carried out by collecting the sea water samples from several peninsulas of Batam Island. The abundance of microalgal diversity was observed. The isolation was done by capillary micropipette technique. The method of specific screening for hydrocarbon-producing microalgae was done using selected media, CO2 rate and light intensity period treatments. The results showed that the Cyanobacteria and diatom occupied the Batams coastal waters. From the isolated samples, 11 strains positively deposit the hydrocarbon in their cells. Further study for determining the isolates and their properties is still in progress.
Isolation and Screening of Surfactant-producing Bacteria from Indonesian Marine Environments and Its Application on Bioremediation Susilaningsih, Dwi; Okazaki, Fumiyoshi; Yopi, Yopi; Widyastuti, Yantyati; Harayama, Shigeaki
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.383 KB) | DOI: 10.1234/80

Abstract

Isolation and screening have been undertaken on oil-degrading microbes from Indonesian marine environments. During screening process it has been found many bacterial isolates capable of degrading crude oil. Hence, study has been focused on the biodiversity of biosurfactant-producing bacterial species in Indonesian marine environment and its function for remedial the pollutant in marine and soil areas. A total of 103 out of 463 isolates showed positive surfactant-degrading properties. By means of partial 16S rRNA gene analyses, it has been found that the majority of taxa are related to Alcanivorax, Pseudomonas, Bacillus, Bortetela, Brucella, Acenitobacter, Staphia, Lysobacter, and Talasosophira. Biosurfactant properties assay showed that they were capable of lowering the surface- and interfacial water tension from 74 mN/m to 40-65 mN/m and from 24 mN/m to 6-10 mN/m, respectively. In addition, most of the surfactants were capable of emulsifying hydrocarbon (crude oil) of 0.01 to 0.15 units, comparable to 0.08 units of synthetic surfactant (20% Tween). Further observation showed that the majority of the surfactants were able to degrade a long chain of alkane, but not branched alkane, with a recovering rate of 20-80%. The application of the surfactant towards oil polluted model beach was done in laboratory scale and showing the surfactant obtained from microbial broth cultures capable for recovering the oil pollutant significantly, compared to the control (without addition microbial broth).  
Molecular Identification of Microalgae BTM 11 and its Lectin Isolation, Characterization, and Inhibition Activity Mustopa, Apon Zaenal; Isworo, Rhestu; Nurilmala, Mala; Susilaningsih, Dwi
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v20i2.251

Abstract

BTM 11 is unknown species of microalgae, but has active compounds that can inhibit viruses. One of proteins produced by microalgae is a lectin. Lectin is a carbohydrate-binding protein found in various microalgae that show antiviral and antibacterial activity. The purpose of this study was to perform identification of the species of microalgae BTM 11, isolation, characterization, and assay of lectin inhibitory activity. Microalgae BTM 11 was identified to have homology with Cyanobacterium 99% and Geitlerinema sp 98%. Lectin of microalgae BTM 11 was isolated by ammonium sulfate precipitation of 75% with a molecular weight of 17 kDa. Lectin protein activity of microalgae BTM 11 was able to inhibit the enzyme activity of RNA helicase hepatitis C by 57.90% and 27.55%. In addition, the protein was able to suppress the activity of Staphylococcus aureus ATCC 6538, E. coli EPEC K.1.1. and Salmonella typhii ATCC 25241. Activitiy of lectin was stable at 30 °C and was unaffected by the action of the enzyme. These results indicate that lectin of microalgae BTM 11 could be a alternative to antiviral and antibacterial proteins.
Influence of Light Intensity on Lipid Productivity and Fatty Acids Profile of Choricystis sp. LBB13-AL045 for Biodiesel Production Praharyawan, Swastika; Rahman, Delicia Yunita; Susilaningsih, Dwi
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.5 KB) | DOI: 10.21776/ub.rjls.2018.005.02.7

Abstract

The influence of various light intensity on the lipid productivity, fatty acids profile, and biodiesel properties of Choricystis sp. AL045 were examined. Choricystis sp. LBB13-AL045 was a high lipid content microalgal strain (up to 42.49 ± 0.41% per dry weight basis) with great performance of its growth (specific growth rate and biomass productivity were up to 0.802 ± 0.013 day-1 and 108.57 ± 8.07 mg L-1 day-1, respectively). Such results indicated its high lipid productivity and its potency to be used for biodiesel production. The treatment of various light intensities on the microalgal culture resulted obvious differences in lipid productivity and fatty acids composition. Maximum lipid productivity (46.13 ± 3.43 mg L-1 day-1) was at 405 µmol m-2 s-1 of light intensity. The fatty acids profiles of four light intensities treatments were quite similar; the major components of fatty acid obtained from Choricystis sp. LBB13-AL045 oil were oleic acid (C18:1) and palmitic acid (C16:0), which provided a strong indication of high-quality biodiesel. Biodiesel properties were determined by empirical equations and found to be within the limits of biodiesel standard SNI 7182:2015, ASTMD6751-08 and EN14214.