DWI NINGSIH SUSILOWATI
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111

Published : 19 Documents
Articles

Found 19 Documents
Search

SPECIES AND FUNCTIONAL DIVERSITY OF RHIZOBACTERIA OF RICE PLANT IN THE COASTAL SOILS OF INDONESIA Susilowati, Dwi Ningsih; Sudiana, I Made; Mubarik, N.R.; Suwanto, A.
Indonesian Journal of Agricultural Science Vol 16, No 1 (2015): April 2015
Publisher : Indonesian Center for Agricultural Library Technology Dissemination - IAARD

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v16n1.2015.39-50

Abstract

Rhizobacteria are important components of soil and directly or indirectly influence the soils quality and plant growth for maintaining adequate plant nutrition and reducing the negative environmental effects of fertilizers. Applying high dose of chemical fertilizers in most of rice fields in the coastal areas could reduce the quality of the soil in the long time. There are few studies addressed to verify the species and functional diversity of cultivable rhizobacteria associated with rice plant in the coastal soils. The objective of the study was to verify the species and functional diversity of rhizobacteria isolated from the coastal soils of two rice production areas of Subang and Indramayu, West Java. Special focus was given to verify phosphate solubilization, nitrogen fixation, IAA and cellulase production of the selected 78 strains of rice rhizobacteria isolated from the coastal paddy field, as well as taxonomical analyses based on 16S rRNA. The results showed that among 78 bacterial isolates from the coastal paddy field, mostly were belonging to the Firmicutes, most of them affiliated with genera Bacillus, 75 strains produced IAA, 32 strains fixed nitrogen, 37 strains solubilized phosphate and 33 strains produced cellulase. Several strains of the rhizobacteria were capable of producing plant growth promoting substances (PGPR), alone or in combination, such as IAA, fixing nitrogen,  solubilizing phosphate, and producing cellulase. Taking all of these diverse PGPR characteristics into account, it is clear that the 78 identified isolates have great potential for improving saline soils of the coastal paddy fields in Indonesia.
WAKTU INKUBASI PADA DERAJAT DISTILASI KITOSAN ENZIM DAN EFEKTIFITAS PENGHAMBATANNYA TERHADAP PENYAKIT ANTRAKNOSA Suryadi, Yadi; Priyatno, Tri Puji; Samudra, I Made; Susilowati, Dwi Ningsih; Nurzulaika, Hermawati; Syaefudin, Syaefudin
Jurnal Fitopatologi Indonesia Vol 12 No 6 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.716 KB) | DOI: 10.14692/jfi.12.6.209

Abstract

The use of chitosan as a coating agent of harvested fruits is an alternative method in controlling anthracnose disease (Colletotrichum sp.). This study aimed to obtain an optimal enzymatic chitosan (EC) that hydrolyzed using chitinase from Burkholderia cepacia isolate E76. The optimal incubation condition to produce EC was 2 h with the yield of 3.52 ± 0.38 g. The degree of deacetylation (DD) chitosan and  EC was 66.91% and 80.91%, respectively. Based on in vitro assays, EC 2% was the most effective in inhibiting the growth of Colletotrichum sp. (94.22%)  than chitosan, while the highest inhibition for chitosan 3% was 55.26%. Moreover, the EC 2% showed the highest inhibition of spore germination (74.12%). The in vivo assay revealed that EC 2% showed the highest inhibition on the fungal growth (88.88%), compared to the other concentrations. On the other hand, the EC 2% and 3% gave similar results on inhibition of Colletotrichum sp.of chili (55.55%). 
KOMUNITAS BAKTERI RHIZOSFER TEH MELALUI APLIKASI BIOIMUNIZER (CHRYSEOBACTERIUM SP. DAN ALCALIGENES SP.) DENGAN METODE TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM (T-RFLP) Aviani, Elin Savitri; Susilowati, Dwi Ningsih; Jannah, Siti Nur; Kusumaningrum, Hermin Pancasakti
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Types of bacteria from the rhizosphere communities actually have been developed as bioimunizer but their consistency is still need further experiment about indigenous bacteria associated with rhizosphere of plants. This study aims to identification on the tea plant rhizosphere bacterial communities which in previous studies has been infected with E. vexans Massee and given bioimunizer as well as the checking existence Chryseobacterium sp. and Alcaligenes sp. The result research showed that communities of bacteria on control samples obtained through culturing Bacillus sp. (51.91%), Acidobacteria bacterium (39.42%) and Actinobacteria sp. (8.66%). Control sample through metagenom obtained Gemmatimonas aurantiaca (5.80%), Bacillus sp. (42.55%), Acidobacteria bacterium (23.45%) and Actinobacteria sp. (28.20%). Communities of bacteria in the samples treated by culturing obtained Gemmatimonas aurantiaca (3.58%), Bacillus sp. (30.76%), Pseudomonas sp. (5.55%) Acidobacteria bacterium (13.94%) and Actinobacteria sp. (46.16%). Communities of bacteria in the samples treated by metagenom found Bacillus sp. (10.66%), Acidobacteria bacterium (4.22%), Actinobacteria sp. (5.48%), Uncultured bacterium (1.49%), Alcaligenes sp. (36.95%) and Chryseobacterium sp. (46.82%). The existence of Alcaligenes sp. and Chryseobacterium sp. show consistency bioimunizer composition is applied. Communities of bacteria on metagenom have diversity and evenness level higher than the culturing approach. Keywords: bacterial communities, T-RFLP, Chryseobacterium sp., Alcaligenes sp.
Identifikasi Entomopatogen Bakteri Merah pada Wereng Batang Coklat (Nilaparvata lugens Stål.) Priyatno, Tri Puji; Dahliani, Yohana A; Suryadi, Yadi; Samudra, I Made; Susilowati, Dwi Ningsih; Rusmana, Iman; Wibowo, Baskoro S; Irwan, Cahyadi
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indentification of Entomopathogenic Red Bacterial fromBrown Planthopper (Nilaparvata lugens Stål.). Tri P.Priyatno, Yohana A. Dahliani, Yadi Suryadi, I MadeSamudra, Dwi N. Susilowati, Iman Rusmana, Baskoro S.Wibowo, and Cahyadi Irwan. Red bacteria isolated frombrown planthopper (BPH) has been proven pathogenicagainst BPH and others insects. Application of 106 to 107cells/ml of red bacteria caused 65.6-78.2% mortality of BPH.The 50% effective concentration (EC50) and lethal time of redbacteria against BPH is 2.8 x 105 cells/ml and 6.8 days,respectively. Based on phenotypic characters tested on GNMicroPlateTM Biolog kit and 16S rRNA sequneces analysis,red bacteria was identified as Serratia marcescens with 99%similarity. Red pigmen produced by S. marcescens strainBPH is secondary metabolite determined as prodigiosinshowing bactericidal activities against Xanthomonas oryzaepv. oryzae. We concluded that S. marcescens did not onlypotent as biocontrol agent to BPH, but also it can be used tocontrol plant pathogenic bacteria.
Isolasi dan Karakterisasi Aktinomisetes Penghasil Antibakteri Enteropatogen Escherichia coli K1.1, Pseudomonas pseudomallei 02 05, dan Listeria monocytogenes 5407 Susilowati, Dwi Ningsih; Hastuti, Ratih Dewi; Yuniarti, Erny
Jurnal AgroBiogen Vol 3, No 1 (2007): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Isolation and Characterization of Actinomycetes ProducingAntibacterial Compound into EnteropatogenikEscherichia coli K1.1, Pseudomonas pseudomallei 02 05and Listeria monocytogenes 5407. Dwi N. Susilowati,Ratih D. Hastuti, and Erny Yuniarti. The resistance ofbacterial pathogens to some antibacterial agents and sideeffects of the antibacterial usage demanded discovery ofnew effective, safe, and active antibacterial compounds.Some pathogenic bacteria, such as enteropathogen Escherichiacoli (EPEC) that cause diarrhoea on children andinfants, Pseudomonas pseudomallei that cause melioidosison human and animal, and Listeria monocytogenes thatcause listeriosis on newly born babies mortality and death ofpregnant woman. Actinomycetes is the largest bacterialgroup that produce antibiotics. More than 10,000 antibacterialcompounds had been discovered, two-third ofthem were produced by this bacterial group. A study wasdone to isolate and characterize Actinomycetes producingantibacterial compounds effective against EPEC K1.1 and P.pseudomallei 02 05. Soil samples were taken from 39locations in Indonesia and 115 actinomycetes isolates wereobtained. Two of the isolates, i.e., isolate A3.5 that waseffective against P. pseudomallei 02 05 and isolate F6.1 thatwas effective against EPEC K1.1 evaluated further. Theisolate A3.5 had an optimum time 72 hours to produce antibacterialcompound, while F6.1 took 96 hours. The antibacterialcompounds produced by both isolates were dissolvein the a 70% ethyl acetate solution, but not in a 40oCwarm methanol solution because it is very dissolved. Theantibacterial compound extracted from the isolate A3.5 hada similar effectiveness to antibiotics bacithracyn 10 unit andneomycin 30 g. On the other hand, the antibacterialcompound extracted from isolate F6.1 had a similar effectivenessto antibiotics colistin 10 g and doxyciclin 30 g.Further identification of the isolates suggested that both ofthem belongs to the genera Streptomyces.
AKTIVITAS ANTICENDAWAN BACILLUS CEREUS 11UJ TERHADAP RHIZOCTONIA SOLANI DAN PYRICULARIA ORYZAE Suryadi, Yadi; Samudra, I Made; Priyatno, Tri Puji; Susilowati, Dwi Ningsih; Lestari, Puji; Sutoro, Sutoro
Jurnal Fitopatologi Indonesia Vol 11 No 2 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.705 KB) | DOI: 10.14692/jfi.11.2.35

Abstract

Microbial secondary metabolites is an important resource for antifungal development in disease control strategy. The objective of this study was to screen Bacillus cereus 11UJ, an antagonistic rhizosphere bacteria for potential secondary metabolite production against rice fungal pathogens, i.e. Rhizoctonia solani and Pyricularia oryzae. The antagonistic effect of crude exract was evaluated using sterile filter paper discs on PDA medium. The ethyl acetate extracts of the bacterium showed a better antifungal activity to P. oryzae than those of R. solani. The inhibitory effect of the filtrate proved the potency of the isolates to produce antifungal. Analysis of pyrolysis gas chromatography-mass spectrometry showed that B. cereus 11UJ produces 3 major compounds i.e; 9,19-cyclolanostan-3-ol, acetate, (3.beta.)- (CAS) cycloartanyl acetate (13.14%); 4-(2?,2?-dimethyl-6?-methyliden-1?-cyclohexyliden)-3-methyl-2-butanone (9.72%); and stigmast-5-en-3-ol oleat (9.09%) which suggested to play an important role in the suppression of rice fungal pathogens.  
Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques Susilowati, Dwi Ningsih; Fauziah, Fani; Pranoto, Eko; Hidayat, Ernin; Setyowati, Mamik; Rachmiati, Yati
Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i2.114

Abstract

Bio-imunizer contains an active compound of  Chryseobacterium sp. and Bacillus sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is Terminal Restriction Fragment Length Polymorphism based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielous evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that Chryseobacterium sp. and Bacillus sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.
Potency of tea plant indigenous microbe on plant growth and to against blister blight disease (Exobasidium vexans Massee) Fauziah, Fani; Setiawati, Mieke Rochimi; Susilowati, Dwi Ningsih; Pranoto, Eko; Rachmiati, Yati
Jurnal Penelitian Teh dan Kina Vol 19, No 1 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v19i1.77

Abstract

The chemical control method of blister blight (Exobasidium vexans Massee) on tea could inflict various negative impacts. In order to obtain an environmentally sound control method of blister blight disease, a nursery trial has been conducted to know the effectiveness of bacterial combinations. The trial was carried out at Gambung experimental garden, with seven treatments and four replications. The indigenous microbial codes are Azoto II-1, Endo-5 and Endo-76. The treatments tested comprised: control (without bacteria),  Azoto II-1 25% + Endo-5 75%; Azoto II-1 50% + Endo-5 50%; Azoto II-1 75% + Endo-5 25%;  Azoto II-1 25% + Endo-76 75%; Azoto II-1 50% + Endo-76 50%; and Azoto II-1 75% + Endo-76 25%. All of the treatments was applied as a soil drench, 50 ml/plant with bacterial concentration at 0,5%. The parameter observed was blister blight disease intensity, plant heights, stem diameter, leaves number, root length, and root volume. The results showed that the combination of Azoto II-1 75% + Endo-5 25% could suppress the intensity of blister blight disease with disease intensity 1.27%. The treatments also affected plant heights, stem diameter, leaves number, root length, and root volume, 15.32 cm; 3.38 cm; 8.05 cm; 18.25 cm and 2.37 cm, respectively.
EKSPLORASI DAN KARAKTERISASI ENTOMOPATOGEN ASAL BERBAGAI INANG DAN LOKASI [Exploration and Characterization of Entomopathogenic from Various Host and Location] Priyatno, Tri Puji; Samudra, I Made; Manzila, Ifa; Susilowati, Dwi Ningsih; Suryadi, Yadi
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3631.714 KB) | DOI: 10.14203/beritabiologi.v15i1.2859

Abstract

Microbial groups of entomopathogenic (fungi and bacteria) had been reported causing insect mortality. The aim of the study was to explore and characterized entomopathogenic from various host and locations. Fungal identification at genus and species level was caried out based on conidial morphology, hyphal growth, conidiophore and colony color; whilst for bacterial identification was based on standard Bergey’s manual for determinative bacteria. Sixteen entomopathogenic isolates that consisted of fungal and bacteria have been collected and preserved for further characterization. Of the 16 entomopathogen collected samples, five fungal genera was found i.e. Paecilomyces; Metarhizium, Beauveria, Hirsutella; and Cordyceps. Seven isolates belonging to six fungal isolates, and one bacterial isolate had been identified based upon ITS and 16S rDNA sequences, respectively. We confirmed that 6 fungal isolates belong to species of Paecilomyces reniformis, B. bassiana, M. anisopliae, M. anisopliae var acridum, Hirsutella thomsonii. One isolate of red pigmented bacteria Sm201102 have been identified was belonging to species Seratia marcescence. It was also obtained two fungal isolates from different host (spider and beetle) which confirmed by morphological character belong to Cordyceps sp.
KOMUNITAS RHIZOBAKTERIA TANAMAN TEH DENGAN APLIKASI FORMULA BIOIMUNIZER (CHRYSEOBACTERIUM SP DAN BACILLUS SP) BERDASARKAN GEN 16S RRNA Widaranti, Agnistisya; Jannah, Siti Nur; Kusumaningrum, Hermin Pancasakti; Susilowati, Dwi Ningsih
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Commodity of tea (Camellia sinensis) has an important role in the national economy, especially in the field of agro-industries. Based on data obtained from the Directorate General of Plantation, the tea plant productivity declined over the last few years. This is due to the pest attack which result in decreasing the productivity of the tea plant. Increased crop productivity of tea have been done, such as the use of herbicides and insecticides, but until now there is very little effort to increase the production of tea plants by the use of biological agents. Chryseobacterium sp and Bacillus sp are rhizobacteria in tea rhizosphere that could potentially be used as a biocontrol agent (bioimmunizer). The purpose of this study is to determine the community rhizobacteria in tea soil with the addition of bioimmunizer based on 16S rRNA gene using T-RFLP technique. The method used in this research is T-RFLP technique (Terminal Restriction Fragment Length Polymorphism) using enzymes Msp I and Rsa I. The calculated value are relative abundance, Shannon diversity index (H '), evenness index (E), and dominance index. The results of this study indicate that soil samples with the addition of bioimunizer consisting of Arthrobacter sp, Bacillus sp, Actinobacteria, and Chryseobacterium sp.Keyword: T-RFLP, Chryseobacterium sp, Bacillus sp