I GEDE KETUT SUSRAMA
Agricultural Pests and Diseases Department, Agroecotechnology Study Program, Faculty of Agriculture, Udayana University

Published : 15 Documents
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Journal : International Journal of Biosciences and Biotechnology

IDENTIFICATION OF ARBUSCULAR MYCORRHIZAL FUNGI IN RHIZOSPHERE SOIL OF SEVERAL GRASS SPECIES AND CACAO (THEOBROMA CACAO L.) BASED ON ITS SPORE MORPHOLOGICAL CHARACTERISTICS Sintya Dewi, Ni Kadek; Susrama, I Gede Ketut; Sritamin, Made; Adnyana, Made; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Arbuscular mycorrhizal fungi live in a symbiotic mutualism with plant roots and helps plant absorbnutrients and also able to live in various places. The study aimed to identify arbuscular mycorrhizal fungiin rizhosphere of several grass and cacao through microscopic method based on morphologicalcharacteristics and was conducted from December 2013 to March 2014. The methods used in this studywere spore isolation using wet sieving and root staining techniques. The results showed that arbuscularmycorrhizal fungi spores found in Imperata cylindrica L. were spores of Glomus (Glomales: Glomeaceae)with its vesicular and hyphae structure. The presence of spores and structures in Paspalum notatum wereAcaulospora (Glomales: Acaulosporaceae) and Gigaspora with its arbuscules, vesicules and hyphae structure.Spores and structures found in the Pennisetum purpureum were belong to genus of Glomus with hyphaeand arbuscular structure. Spores and structures of mycorrhizal fungi in Cyperus rotundus are spores of thegenus of Gigaspora (Glomales: Gigasporineae) and spores of Glomus with internal hyphae structure. Whilespores and mycorrhizal structures in Cacao were found two types of spore belong to genus of Glomus withhyphae and vesicular structures.
CROP IMPROVEMENT THROUGH INDUCING MUTAGENESIS IN VIVO USING COLCHICINE ON COWPEA (VIGNA UNGUICULATA L. WALP) Susrama, I Gede Ketut; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2017.v04.i02.p02

Abstract

Colchicine treatments of 0.005% and 0.01% either in distilled water or in hydrogen peroxide 3% were caused temporary leaf shape changes on cowpea. Treatment of colchicine in hydrogen peroxide 3% resulted in green pod color change to purple however treatment of just hydrogen peroxide 3% and colchicine in hydrogen peroxide 3% caused 8.8-10.7% dwarf cowpea. As a preliminary data, we also observed that cowpea crops grown from seeds treated with colchicine in distillate water attacked by bean leaf beetle much earlier compare to the treatment with colchicine in hydrogen peroxide 3%.
ISOLATION AND IDENTIFICATION OF ARBUSCULAR MYCORRHIZAL FUNGI (AMF) IN CASHEW PLANTS (ANACARDIUM OCCINDENTALE L.) IN DATAH VILLAGE, ABANG DISTRICT OF KARANGASEM REGENCY Suada, I Ketut; Prima, Evy; Sritamin, Made; Adiartayasa, I Wayan; Susrama, I Gede Ketut; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2018.v05.i02.p10

Abstract

Isolation and identification of arbuscular mycorrhizal fungi (AMF) in cashew plants was carried out in Datah Village, Abang District of Karangasem Regency. This study aims to determine the diversity of mycorrhizal spores in the cashew rhizosphere and AMF infections in the roots of cashew plants. The result in microscopic observations found 5 genera of AMF spores such as Glomus, Racocetra, Entrophospora, Septoglomus, and Acaulospora and the population of the genus Glomus were found most dominant in the rhizosphere of cashew plants. The result of staining root with trypan blue obtained the internal structure of AMF in the form of vesicles, arbuscules and hyphae found in root tissue of cashew plants. The result of AMF DNA amplification with primer NS31 and AML2 showed 560 bp bands in electrophoresis with 1% agarose gel determined AMF infection in root of cashew plants.
IN VIVO MULTISTEP MUTAGENESIS INDUCTION USING COLCHICINE ON COWPEA MUTANT 1 (VIGNA UNGUICULATA L. WALP) Susrama, I Gede Ketut; Wirawan, I Gede Putu
International Journal of Biosciences and Biotechnology Vol 5 No 2 (2018)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2018.v05.i02.p04

Abstract

In the previous research, we obtained three catagories of M1 mutant cowpea namely 1) purple pod mutant cowpea, 2) green pod mutant cowpea which has three pods in a stalk, and 3) green pod mutant cowpea which has two pods in a stalk. All those three catagories of M1 mutant cowpeas were treated again with colchicine in a multistep mutagenesis proses in vivo. Then, we found changes in number of leaflet in a petiole to 4 leaflets (quadrifoliate) and to five leaflets in a petiole (pentafoliate). These changes are indications that beside as a chromosome multipying alkaloid, colchicine causes other genetic changes as well. Through this mutagenesis research, we created gigas mutant cowpea that having longer leaves, longer pods and heavier seeds. The accurrence of two kinds of insect pests attack were found namely cowpea aphid Aphis craccivora and pod sucking bug Riptortus linearis.
AGARWOOD PRODUCING FUNGAL INOCULANT FORMULATION IN KETIMUNAN TREE (GYRINOPS VERSTEEGII DOMKE) Mega, I Made; Suanda, Dewa Ketut; Kasniari, Desak Nyoman; Susrama, I Gede Ketut
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This study aimed to find out the agarwood producing fungal inoculant formulation that infect Ketimunan tree (Gyrinops versteegii DOMKE) effectively and resulted in high quality of agarwood. Randomized Completely Block Design was applied in this study with formulation of Fusarium solani and Rhizopus sp inoculant in liquid and solid form were combined as treatments. Treatments were: solid Fusarium solani inoculant, solid Rhizopus sp inoculant, mixture of solid Fusarium solani inoculant and solid Rhizopus sp inoculant, liquid Fusarium solani inoculant, liquid Rhizopus sp inoculant, mixture of liquid Fusarium solani inoculant and liquid Rhizopus sp inoculant, and mixture of solid and liquid of Fusarium solani and Rhizopus sp inoculants. There are three replicates for each treatment. Parameters measured were level of fragrance, agarwood color, and its resin content. The result showed that all formulations affected resin content significantly compared to control of agarwood. Mixture of liquid Fusarium solani and liquid Rhizopus sp inoculant showed the best quality agarwood with quality characteristics: brownish black or agarwood with black and brownish black color, very strong fragrance, and with 13.58% resin content.