I GEDE KETUT SUSRAMA
Agricultural Pests and Diseases Department, Agroecotechnology Study Program, Faculty of Agriculture, Udayana University

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Journal : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

ISOLASI DAN KARAKTERISASI CENDAWAN MIKORIZA ARBUSKULAR PADA TANAH DAN AKAR TANAMAN JAGUNG DI DESA SANUR KAJA DIASTAMA, I WAYAN PRASTITA; SUSRAMA, I GEDE KETUT; WIRAWAN, I GEDE PUTU
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Characterization of Mycorrhizae Arbuskular Fungi in the Soil and Roots of Corn (Zea Mays L.) in the Sanur Kaja Village Mycorrhiza is an organism that is derived from the fungus that describes a form of symbiotic relationship between mutualisme functions with a high degree of plant roots. Benefits of mycorrhiza for plant growth and development as its host is to increase the absorption of nutrient elements of soils, as biological barrier against infection of root pathogen, enhancing the resilience of crops to drought and increasing hormone boosters grows.This research aims to identify a Arbuskular Mycorrhiza fungi isolated from corn plants rhizosphere in the village of Sanur Kaja. The results of this research show that the isolation and characterization of spores that develop on the roots of corn in the village of Sanur Kaja indicates a growing spores there is the genus Glomus sp. type of Spore that successfully identified a species of Glomus sp-1, Glomus sp-2, Glomus sp-3. Key words:Glomus sp, rhizosphere, symbiotic mutualisme
ISOLASI DAN IDENTIFIKASI AGROBACTERIUM TUMEFACIENS PADA TANAMAN MAWAR (ROSA SP.). SILITONGA, NADIAH; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens At Plant roses (Rosa sp.). Agrobacterium tumefaciens caused crown gall tumor in many of dicotyledonous plants. The purpose of this study tried to isolate and identify the A. tumefaciens from rose plant. Various techniques were used such as selection media, colonies shape and color, Koch's postulate test, DNA isolation and agarose gel elektroforesis. The results of this study showed that A. tumefaciens can be isolated through culturing in LB medium and selection AB medium that was specific for A. tumefaciens. The isolate caused crown gall tumor on carrot slice 3 weeks after inoculation by using a modified Koch?s postulate test. The characteristics of colony formed in this research are round shaped, cream coloured with pink tint, smooth edge, and convex elevation. DNA isolation and its running in agarose gel electroforesis showed positive result.   Key words : Agrobacterium tumefaciens, AB medium and Agarose gel electroforesis.
ISOLASI DAN IDENTIFIKASI AGROBACTERIUM TUMEFACIENS DARI TANAMAN WORTEL (DAUCUS CAROTA L.) MANALU, YOLANDA HASSIAN; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Isolation and Identification of Agrobacterium tumefaciens from  Carrots (Daucus carota L.) Agrobacterium tumefaciens is a plant pathogenic bacterium that is widely used as a vector for inserting foreign genes into a plant chromosome to produce a transgenic plant. This bacterium cause a disease namely crown gall in dicotyledonous plants. This study is aim to isolate A. tumefaciens from local carrot (Daucus carota L.) by using some method such as selection media, characterization of the bacteria, and confirm by Koch?s postulates. The result of this study determined that carrot?s root taken from Bedugul area which showed crown gall symptom, an A. tumefaciens due to its characteristics e.g. shape of colonies, color, and growth of the bacteria in AB minimal medium that was a specific for A. tumefaciens.  By using the Koch?s postulates test showed that the isolated caused convex gall on the surface of carrot slice. DNA of isolated bacterium successfully electroforeted through agarose gel electrophoresis.  These results showed that the bacteria associated with carrot was A. tumefaciens. Keywords : A. tumefaciens, AB medium, Carrot, LB medium, Crown gall
UJI AKTIVITAS ANTIMIKROBA BEBERAPA EKSTRAK BUMBU DAPUR TERHADAP PERTUMBUHAN JAMUR CURVULARIA LUNATA (WAKK.) BOED. DAN ASPERGILLUS FLAVUS LINK. SITEPU, IRMA SELVYANA Br.; SUADA, I KETUT; SUSRAMA, I GEDE KETUT
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol. 1, No. 2, Oktober 2012
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT The Antimicrobial Activity Test of Some Kitchen Seasoning Extracts on Growth of Fungus Curvularia lunata (Wakk.) Boed. and Aspergillus flavus LINK. This research was aimed to determine the ability of herbs extract in inhibiting the growth of fungus C. lunata and A. flavus and to determine the minimum inhibition concentration of each extracts as well. The results showed that the extracts could inhibit the growth of the two fungus. The most effective extract was turmeric against C. lunata and galangal extract against A. flavus with each inhibition was 38,6% and 26,6% respectively. The minimum inhibition concentration of all extracts were 0,5% on both C. lunata and A. flavus. Keyword : Antimicrobial, Kitchen seasoning, Curvularia lunata, Aspergillus flavus
IDENTIFIKASI FUNGI MIKORIZA ARBUSKULAR SECARA MIKROSKOPIS PADA RHIZOSFER TANAMAN ALANG-ALANG (IMPERATA CYLINDRICA L.) DI DESA SANUR KAJA NAINGGOLAN, ROMAULI THERESIA; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Identification of Mikoriza Arbuskular Fungi in Microscopic the Rhizosphere of Reed (Imperatacylindric L.) in Sanur Kaja Village.   Mycorrhiza is a form of symbiosis between the fungus with a high level of plant (vascular plants, Tracheophyta), especially on the rooting. There is also a fungus with the other symbiotes, but the term mycorrhiza are typically for symbiotic plant roots which infect by fungus. This research aims to know the diversity of the genus or species of  mycorrhiza fungi arbuskular on the rhizosfer plant reeds in the village of Sanur Kaja, and to know whether or not there was colonization of mycorrhiza fungi arbuskular on the net root crops the reeds in the village of Sanur Kaja. The results of this research show that the symbiotes spores in the rhizosfer plant reeds is the genus Glomus multicaule spores, Glomus ambisporum, Acaulospora foveata, Gigaspora gigantae. Analysis on the plant roots mycorrhiza colonization reed showed a spherical structure called vesikular, while arbuskular is a structure on the hypha branches that resemble haustorium. (forming the pattern dikotom).   Key words: Acaulospora sp, Gigaspora sp, Glomus sp.
IDENTIFIKASI MIKORIZA VESIKULAR ARBUSKULAR (MVA) DARI RHIZOSFER TANAMAN CABAI (CAPSICUM ANNUUM L.) DAN TOMAT (SOLANUM LYCOPERSICUM L.) SERTA PERBANYAKANNYA MENGGUNAKAN MEDIA ZEOLIT WIRAWAN, I WAYAN EKA ADI; SUADA, I KETUT; SUSRAMA, I GEDE KETUT
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Vesicular Arbuscular Mycorrhizae (VAM) Identification of Chilli (Capsicum annuum L.) and Tomato (Solanum lycopersicum L.) Rhizosphere and Its Spore Multiplication in Zeolite Media Various attempts have been made to increase the production of chilli and tomato plants by farmers, including by using  inorganic fertilizer application on and on going basis. Considering potential problems that may occured due to inorganic fertilizer use, aplication of  biological fertilizer which one of them is containing vesicular arbuscular mycorrhizae (VAM) could be expected to assist the growth of chilli and tomato in more naturally manner. This study was aimed to determine genus and species of VAM in chilli and tomato rhizosphere, its colonization in root tissue, and to examine zeolite media compatibility with corn as a symbiont. Based on the results of the study, it was found four species namely Acaulospora fofeata, A. colombiana, A. Laevis, and Glomus ambisporum in chilli and four species in tomato that were identified as A. fofeata, A. colombiana, Scutellospora calospora, and G. ambisporum. Colonization were found in roots of chilli, tomato, and corn indicated by symbiotic structures arbuscules, vesicles, and inner spores. Zeolite media with corn as symbiotic plant is considered suitable for VAM spore propagation. Keywords: rhizosphere, Acaulospora fofeata, zeolite, inner spore
IDENTIFIKASI MIKORIZA VESIKULAR ARBUSKULAR (MVA) PADA RHIZOSFER TANAMAN UBI JALAR (IPOMOEA BATATAS L.) DAN UBI KAYU (MANIHOT ESCULENTA CRANTZ) SERTA PERBANYAKANNYA DENGAN MEDIA ZEOLIT WIDIATMA, PUTU SENA; WIRAWAN, I GEDE PUTU; SUSRAMA, I GEDE KETUT
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.4, Oktober 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Vesicular Arbucular Mycorrhizae (VAM) Identification of Sweet Potato (Ipomoea batatas L.) and Cassava (Manihot esculenta Crantz) Rhizosphere and Its Spore Multiplication in Zeolite Media Vesicular arbuscular mycorrhizae is an obligate symbiont that live in a symbiotic mutualism with plant roots and grow inside root cortical cells helping absorption of nutrients which necessary for plant growth. This research aimed to determine VAM species in sweet potato and cassava rhizosphere and also to determine effectiveness of zeolite media as a multiplication medium. The research has been conducted from October 2014 through December 2014. Spore isolation was done by conducting wet sieving method. Roots colonization percentages were calculated with gridline section method and spores multiplication through trapping culture method. Results showed that there were four VAM genera consist of 16 species from rhizosphere of both sample plants. Those four genera identified as Acaulospora (2 species), Gigaspora (1 species), Glomus (1 species), and Scutellospora (1 species) from rhizosphere of sweet potato and 3 genera identified as Acaulospora (3 species), Gigaspora (3 species), and Glomus (5 species) from rhizosphere of cassava. Colonization is characterized by special structure of VAM such as arbucule and vesicule in plant roots tissue. Inner spores of VAM were discovered in all three kind of plants used in this research. Spore multiplication with trapping culture method using zeolite media and corn as a symbionts can be considered as an effective method for VAM spore multiplication indicated by density increase of VAM spores. Keywords : Colonization, Cortical Cell, Inner Spore, Symbiont, Trapping Culture
KEBUTUHAN NUTRISI DAN SUBSTANSI DALAM PAKAN BUATAN SERANGGA (ARTIKEL ULASAN) SUSRAMA, I GEDE KETUT
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.3, Juli 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Insect Nutritional Requirements and Substances in its Artificial Diet In its life, insect as like as other creatures on earth need nutrients for the metabolism in their body so that the larval stage can grow to adult insect and then can do copulation to produce fertile eggs for its life cycle and for the existence sustainability of their species. Nutrional requirements among insects are certainly not the same depending on insect classification and actually different from species to species however in general there are major nutritional requirements that must available in an insect artificial diet. The nutrional requirements of insects and substances that commonly available in an artificial diet composition for insect are discussed in this article review.