Supriyatna Sutardjo
Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Padjadjaran, Jatinangor 45643, Sumedang

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AN ISOFLOVONOID, WARANGALONE FROM THE STEM BARK OF DADAP AYAM (ERYTHRINA VARIEGATA) Herlina, Tati; Nasrudin, Nasrudin; Supratman, Unang; Subarnas, Anas; Sutardjo, Supriyatna; Hayashi, Hideo
Jurnal ILMU DASAR Vol 9 No 1 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

In the course of our continuing search for novel paralytic compound from Indonesian plants, the methanol extract of the stem bark of Erythrina variegata (Leguminosae) showed significant paralytic activity against the third instar larvae of silkworm (Bombyx mori). The purposes of this research were isolation and structural elucidation of paralytic compound from the stem bark of E. variegata. Using the paralytic activity following the separation, the methanol extract was separated by combination of column chromatography to yield prenylisoflavone, warangalone. The chemical structure of warangalone was identified based on spectroscopic evidence and comparison with the previous reported. The paralytic activity of warangalone showed weak activity against the third instar larvae of silkworm (B. mori).
In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant Herlina, Tati; Supratman, Unang; Subarnas, Anas; Sutardjo, Supriyatna; Amien, Suseno; Hayashi, Hideo
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro  test  towards  breast  cancer  cell  T47D.  In  the course  of  our  continuing  search  for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of  E. variegata  showed  significant  anti-cancer  activity  against  breast  cancer  cell  T47D  in  vitro  using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure  of  an  anti-cancer  compounds  were  determined  on  the  basis  of  spectroscopic evidence  and  comparison  with  the  previously  reported  and  identified  as  an  erythrina  alkaloid (1)  and  isoflavonoid  (2).  Compounds  (1-2)  showed  anti-cancer  activity  against  breast  cancer cell  T47D  used  with  IC50  of    1.0  and  3.3  µg/mL,  respectively.  This  results  strongly  suggested that E. variegata is promising sources for anti-cancer agents.Keywords: Anti-cancer, Erythrina variegata, Leguminoseae
AKTIVITAS ANTIMALARIA DAUN ERYTHRINA VARIEGATA Herlina, Tati; Supratman, Unang; Subarnas, Anas; Sutardjo, Supriyatna; Abdullah, Noor Rain
Jurnal Natur Indonesia Vol 10, No 1 (2007)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.10.1.36-41

Abstract

The leaves of Erythrina variegata (Leguminosae) used tradisional plant of an antimalarial. In the course of our continuing search for novel an antimalarial compound from Erythrina plants, the methanol extract of the leaves ofE. variegata showed significant antimalarial activity in vitro toward Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) method. The methanol extract of the leaves of E. variegata showed against bothstrains of parasite with IC50of 6.8 ?g/ml against K1 and > 60 ?g/ml against 3D7, respectively. The methanol extract of the leaves of E. variegata was separated by using bioassay-guide fractionation. The n-buthanol fraction yieldedthe most activity, exhibiting equipotency against both strains of parasite with IC50of 5.1 ?g/ml against K1 and 13.5 ?g/ml against 3D7, respectively. Furthermore, by using the antimalarial activity to follow separation, the n-buthanol fraction was separated by combination of column chromatography to yield an active compound. The active compound showed antimalarial activity against both strains of parasite used with IC50 of 4.3 ?g/ml against K1 and 23.5 ?g/ml against 3D7, respectively. Its inhibition of the resistant strain (K1) was also much better compared to its inhibition of the sensitive strain (3D7), indicated that the leaves of E. variegata to be potential as antimalarial agents, but its lower potency compared to artemisinin and chloroquin.
ANTI-MALARIAL COMPOUND FROM THE STEM BARK OF Erythrina variegata Herlina, Tati; Supratman, Unang; Soedjanaatmadja, M. S.; Subarnas, Anas; Sutardjo, Supriyatna; Abdullah, Noor Rain; Hayashi, Hideo
Indonesian Journal of Chemistry Vol 9, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.169 KB) | DOI: 10.22146/ijc.21547

Abstract

During the course of our continuing search for novel anti-malarial compounds from Indonesian plants, the methanol extract of the bark of E. variegata showed significant anti-malarial activity toward Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) assay. The methanol extract of the bark of E. variegata  was separated by using bioassay-guide fractionation. The ethyl acetate fraction showed the most activity, exhibiting equipotency against both strains of parasite with IC50 of 23.8 µg/mL against 3D7 and 9.3 µg/mL against K1. Furthermore, by using the anti-malarial activity to follow separation, the ethyl acetate fraction was separated by combination of column chromatography to yield an active compound. The chemical structure of active compound was determined on the basis of spectroscopic evidences and comparison with those previously reported and identified as an isoflavonoid, warangalone. The warangalone showed anti-malarial activity against both strains of parasite used with IC50 of 4.8 µg/mL against 3D7 and 3.7 µg/mL against K1.   Keywords: Antimalarial, Erythrina variegata, warangalone
A TRITERPENOID SAPONIN FROM SEEDS OF KOLOWE (Chydenanthus excelsus) Rijai, Laode; Sutardjo, Supriyatna; Bahti, Husein Hernandi; Supratman, Unang; Rumampuk, Rymond J.; Taylor, W.C.
Indonesian Journal of Chemistry Vol 4, No 3 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.279 KB) | DOI: 10.22146/ijc.21854

Abstract

A triterpenoid saponin have been isolated from n-butanol fraction of the seeds of kolowe (Chydenanthus excelsus). The structure was determined as 3-O-[β-D-glucopyranosyl(1→3)-β-D-xylopyranosyl(1→3)- β - D-glucuronopyranosyloxy]- 22α -O-(2-methylbutiroyloxyolean-12-en-15α,16α,28-tri-hydroxy. Structure elucidation was accomplished by NMR (HMBC, HMQC/HSQC, ROE, ROESY, TOCSY) methods, ESIMS, and IR spectroscopic.   Keywords: Chydenanthus excelsus, Lecythidaceae, Triterpenoid saponin
In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant Herlina, Tati; Supratman, Unang; Subarnas, Anas; Sutardjo, Supriyatna; Amien, Suseno; Hayashi, Hideo
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp286-290

Abstract

Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro test towards breast cancer cell T47D. In the course of our continuing search for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of E. variegata showed significant anti-cancer activity against breast cancer cell T47D in vitro using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure of an anti-cancer compounds were determined on the basis of spectroscopic evidence and comparison with the previously reported and identified as an erythrina alkaloid (1) and isoflavonoid (2). Compounds (1-2) showed anti-cancer activity against breast cancer cell T47D used with IC50 of  1.0 and 3.3 µg/mL, respectively. This results strongly suggested that E. variegata is promising sources for anti-cancer agents. Keywords: Anti-cancer, Erythrina variegata, Leguminoseae
TRITERPENOID PENTACYCLIC ANTIMALARIAL ACTIVITY FROM THE LEAVES OF ERYTHRINA VARIEGATA Herlina, Tati; Supratman, Unang; Urbanas, Anas; Sutardjo, Supriyatna; Abdullah, Noor Rain; Hayashi, Hideo
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.786 KB)

Abstract

In the course of our continuing research for novel antimalarial agent from Indonesian plants, the methanol extract of the Erythrina variegata leaves showed significant antimalarial activity against Plasmodium falciparum strain K1 in vitro with IC50 6.8 ?g/mL. The methanol extract was separated by using variety of chromatography techniques. The chemical structure of an antimalarial compound was determined on the basis of spectroscopic evidence and compared to previous data then this compound is identified as a pentacyclic triterpenoid oleane derivative, namely 3,22,23-trihydroxy-oleane-12-ene. The pentacyclic triterpenoid, 3,22,23-trihydroxy-oleane- 12-ene showed antimalarial activity against 3D7 and K1 strains with IC50 4.3 ?g/mL and 24 ?g/mL, respectively. These results strongly suggested that E. variegata is a promising sources of antimalarial agents.