Amalia Sutriana
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EFFECT OF STORAGE TIME OF EPIDIDYMIS AT 5 OC ON SPERMATOZOA QUALITY OF ACEH LOCAL GOAT Hamdan, Hamdan; Panjaitan, Budianto; Sutriana, Amalia; Aliza, Dwinna; Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9811

Abstract

The objective of this study was to evaluate the effect of storage time of epididymis at 5 oC on spermatozoa quality of Aceh local goat. This research was conducted at Reproduction Laboratory, FKH Unsyiah from May to June 2009. Nine epididymis from local goat at the age of 1.5-2.0 years were used in this study. The epididymis were collected from abattoir in Banda Aceh. The experimental design used in this study was completely randomized design with 3 treatments and 3 replications. Spermatozoa quality which consist of sperm concentration, motility, amount of life sperm, and sperm abnormality were examined after the collection of epididymis on day 0 (H-0), day 1 (H-1), and day 3 (H-3) post storage at 5 oC. Data were analyzed using one-way analyses of variance (ANOVA). The result showed that no significant difference (P 0.05) seen in the spermatozoa quality after different storage time. The average of spermatozoa concentration on H-0, H-1, and H-3 were 318x107/ml, 282x107/ml, and 241x107/ml respectively. On the average, the percentage of spermatozoa motility on H-0, H-1, and H-3 were 82,27±2,75; 80,25±2,83; and 78,07±0,92%, respectively. Life spermatozoa observed on H-0, H-1, and H-3 were 82,29±2,71; 80,63±1,87; and 80,09±3,31%, respectively. Observation on spermatozoa abnormality showed that the average of spermatozoa abnormality on H-0, H-1, and H-3 were 7,23±0,27; 8,21±0,55; and 10,75±3,14%, respectively. It could be concluded that the spermatozoa quality were not affected by the storage time at temperature of 5 oC.
IN VITRO ANTIMALARIAL ACTIVITY OF JALOH LEAVES EXTRACT ON PLASMODIUM FALCIPARUM Asmilia, Nuzul; Sutriana, Amalia; Rahmi, Erdiansyah; Sugito, .
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Malaria still cause health problem in tropics including Indonesia, since its generate high mortality rate. Medication of Malaria has been performed long time ago without optimum result. One reason is the high rate of P.falciparum resistance on conventional antimalaria drug. This research aim to evaluate the activity of jaloh leaves extract (Willow leaves) on the inhibition of P.falciparum in vitro.This was a laboratory experimental research that using Indonesian isolates of cloroquin resistance P.falciparum obtained from NAMRU-2, Jakarta. P.falciparum isolate that has been synchronized were divided in to 3 groups of treatment : Jaloh leaves ethanolic extract, jaloh leaves ethyl acetate extract, and jaloh leaves n-hexane extract. Each group were then alloted into 5 dosage levels: 6,25ug/ml; 12,50ug/ml; 25,00ug/ml; 50,00ug/ml; and 100ug/ml. The observation of parasitemia degree were done 48 hours post-incubation in which the thick blood smear were colored with Giemsa and then examined using bioculer microscope. Data were analyzed using one way ANOVA and Newman-Keuls test. The result showed that the percentage of P.falciparum growth inhibition were sinificantly different (P 0.05) among treatments wherein ethanol extract and ethyl acetate extract more inhibit P.falciparum growth than the hexane extract. The administration of different dosage also resulted in significance different (P 0.05) of P.falciparum growth inhibition. This research concludes that jaloh leaves extract could inhibit P.falciparum growth.
THE USE OF CASSAVA LEAVES AS A DIETARY COMPONENT FOR AFRICAN CATFISH FRY Sutriana, Amalia
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3128

Abstract

 An experiment was carried out to evaluate the effect of graded levels of cassava leaf meal (CLM) with varying cyanide content on growth performance of African catfish (Clariasgariepinus) fry. There were four groups of fish, each comprising 12 fish per group. One groupwas fed on a control diet (0 mg HCN/kg diet) with fish meal (FM) and soybean meal (SBM) asprotein source (30% CP). Groups two, three and four were fed graded levels of CLM to replace10, 20, and 30% of the total diet at the expense of SBM which contains 4.19, 7.47 and 11.96 mgHCN/ kg diet respectively. After 12 weeks, fish fed with increasing level of CLM showed asignificant growth depression and poor feed utilization compared to those fed the control diet.Apparent dry matter digestibility (ADMD) and apparent protein digestibility (APD) were alsosignificantly (P 0.05) affected by cassava leaf meal inclusion, with the best values were found infish fed the control diet (52.26+0.24% and 83.11+1.17% for ADMD and APD respectively). Bodycomposition of fish fed higher CLM tended to have less protein and fat but more ash. The poormethionine availability as a result of low protein digestibility and the presence cyanide contentlimits the use of CLM in African catfish fry diet.Keywords: African catfish, cassava, growth, cyanide
CHARACTERIZATION OF INHIBIN FROM CULTURE AND NON CULTURE OF GRANULOSE CELLS FOR MONOCLONAL ANTIBODY OF INHIBIN PRODUCTION Amiruddin, Amiruddin; Nizwan Siregar, Tongku; Sutriana, Amalia; Aliza, Dwinna; Armansyah, T.
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9789

Abstract

This study has long-term objectives to obtain immunogenic prototype that can be used to induce multiple ovulation in goats. Working steps of this study were begun with the collection of ovarium from goats, collection of granulose cells, culture of granulose and characterization of molecular weight and isoelectric point (pI) of inhibin protein of granulose cells obtained from culture and non-culture of granulose cells, and followed by preparation of monoclonal antibody toward inhibin. The results showed that inhibin isolated either from culture or non-culture of granulose cells produced a 32 kDa band. Molecular weight of inhibin was measured by Western Blot. The 32 kDa band of SDS PAGE product appeared on Western Blot result was inhibin molecules produced by granulose cells collected fom culture and non-culture of granulose cells that can be identified by Mab-inhibin. Product of IEF gel electrophoresis suggested that inhibin molecule collected from culture of granulose cells has no charge at isoelectric points ranging from 5-6, depends on its total amino acid composition.
UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (PHYLLANTUS EMBLICA) TERHADAP MENCIT (MUS MUSCULUS). (ACUTE TOXICITY TEST OF ETHANOLIC EXTRACT OF MALAKA (PHYLLANTUS EMBLICA) LEAVES ON MICE (MUS MUSCULUS)) TR, T. Armansyah; Indriany, Sudi; Sutriana, Amalia; Rosmaidar, Rosmaidar; Asmilia, Nuzul; Panjaitan, Budianto; Aliza, Dwinna; Hamdan, Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5137

Abstract

ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.
EFFECT OF SALINITY CHANGES ON METHIONINE CONTENT IN TIGER GROUPER JUVENILE (EPINEPHELUS FUSCOGUTTATUS) Abbas, Muhammadar Abdullah; Abidin, Muchlisin Zainal; Putra, Dedi Fazriansyah; Firdus, Firdus; Batubara, Agung Setia; Sail, Asmawati Muhammad; Syafruddin, Syafruddin; Roslizawaty, Roslizawaty; Sutriana, Amalia; Aliza, Dwinna
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.12363

Abstract

This study was conducted to determine the effect of water salinity changes on methionine content in tiger grouper juvenile (Epinephelus fuscoguttatus). A total of 2,560 tiger grouper juveniles were used and divided into five groups consist of 1 control group (without exposed to salinity changes) and 4 treatment groups. The salinity was changed every 2, 3, 4, and 6 hours in treatment A, B, C, and D, respectively. Salinity levels were changed during 24 hours by lowering salinity level from 32 psu to 22 psu. Twenty five of fish were collected from each treatment for methionine content analysis. Data were analysed using one way analysis of variance (ANOVA). The results showed that there was a decrease in methionine content in all treatments. A significant decrease (P 0.05) of methionine content in treatment A, B, and C were observed after 20 hours (1.15%), 18 hours (1.27%), and 16 hours (1.24%), respectively. While at 0 hours (control), the methionine content was 2.02%. Methionine level in treatment D was not significantly different (P 0.05) compared to control group. As conclusion, rearing the tiger grouper juvenile with salinity fluctuations every 6 hours did not lead to methionine deficiency.
EFFECTIVENESS OF THE FERMENTATIVE EXTRACT OF LACTOBACILLUS ACIDOPHILUS AS ANTIMICROBIALS AGAINST AEROMONAS HYDROPHILA Akter, Mst Nahid; Hashim, Roshada; Sutriana, Amalia; Mohd Nor, Siti Azizah
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.11920

Abstract

The purpose of this study was to evaluate the in vitro and in vivo antimicrobial activity of the commercial Lactobacillus acidophilus (L. acidophilus) cells and cell free extract against Aeromonas hydrophila (A. hydrophila). The in vitro method was carried out using well diffusion method. For in vivo evaluation, the effect of L. acidophilus on the survival rate of Pangasianodon hypophthalmus (P. hypophthalmus) infected with A. hydrophila was evaluated. The well diffusion method showed a significant inhibition ability of L. acidophilus cells against A. hydrophila compared to the cell free extract. The inhibition diameters obtained with cells and cell free extract were 17.23 mm and 15.17 mm, respectively. P. hypophthalmus injected with L. acidophilus cells and cell free extract following challenged with A. hydrophila cells showed survival rate of 70% and 60% respectively, at 2-week post challenged. The gas chromatography-mass spectrophotometry (GC-MS) result revealed that a diverse of compounds was detected in both the L. acidophilus cells and cell free extract, among them the most abundant component was pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl), which showed a promising anticancerous activity and might be played a significant role in the recovery of the infectious P. hypophthalmus. The current study revealed that both cells and cell free extract of L. acidophilus have antimicrobial activity against A. hydrophila.
UJI PREKLINIS FRAKSI ETIL ASETAT DAUN JALOH (SALIX TETRASPERMA ROXB) SEBAGAI ANTIPLASMADIUM SECARA IN VITRO Asmilia, Nuzul; Sugito, Sugito; Sutriana, Amalia
Jurnal Sain Veteriner Vol 29, No 2 (2011): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1094.727 KB) | DOI: 10.22146/jsv.39532

Abstract

The aim of the present study was to find out the in vitro activity of ethyl acetate fraction of willow leaves (Salix tetrasperma Roxb) on Plasmodium folcifarum (P. falciparum) growth.
UJI TUBERCULIN PADA KULIT MONYET EKOR PANJANG (MACACA FASCICULARIS): DALAM UPAYA PENCEGAHAN PENYAKIT ZOONOSIS TUBERKULOSIS (TBC) DI KAWASAN WISATA PULAU WEH SABANG Rahmi, Erdiansyah; Sutriana, Amalia; Aliza, Dwina
Jurnal Primatologi Indonesia Vol. 7 No. 1 (2010)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7643.294 KB)

Abstract

Abstrak: Penelitian ini bertujuan untuk memperoleh informasi tentang penularan penyakit tuberkulosis (TBC)  pada monyet ekor panjang (Macaca fascicularis) di kawasan wisata Pulau Weh, Sabang dan untuk mendukung program pemerintah dalam menghasilkan Indonesia bebas TBC. Penelitian ini dilakukan dalam beberapa tahap setelah memperoleh persetujuan dan izin dari Balai Besar Konservasi dan Sumberdaya Alam Aceh dan instansi terkait di Sabang. Tahapan berikutnya menentukan lokasi, populasi, dan identifikasi monyet ekor panjang serta menentukan jumlah monyet ekor panjang per kelompok. Pemeriksaan hewan dengan melakukan sedasi menggunakan ketamin (10mg/kg) dan xylazine (2 mg/kg) secara intramuskuler. Skrining menggunakan tes tuberkulin metode (mammalian old tuberculin) dilakukan terhadap semua hewan 0,1 mL (135000 Tuberkulin Unit) secara intradermal pada palpebra. Hasil uji tuberculin dibaca pada 24, 48, dan 72 jam pasca suntik. Pengujian diulang pada minggu kedua. Data ditabulasi dan dianalisis secara deskriptif. Dari identifikasi populasi monyet di dua wilayah itu ditemukan satu kelompok monyet pada kilometer nol (N 05 ° 54 '19,1 "E 95 ° 12' 57,4") dengan populasi 14 ekor, sedangkan di wilayah Cot Murong ( N 05 ° 54 '19,1 "E 95 ° 12' 57,4") ditemukan dua kelompok dengan populasi 12 dan 14 ekor. Skrining tuberkulosis menggunakan metode ini menunjukkan bahwa tidak ada insiden TBC pada monyet ekor panjang di kawasan wisata Sabang.Abstract: This research was purposed to obtain the information about the evidence of Tuberculosis (TB) infectious disease on long tail macaque (Macaca fascicularis) in tourism area Weh Island, Sabang and to support the government?s program in generating Indonesia free TB, as well. This research was conducted on many stages, those were permits arrangement with BKSDA Aceh and Forestry Officer Sabang, location identification of long tail macaque populations, and identification of the amount of long tail macaque population in each groups. The monkeys chosen were restrained and anaesthetized using Ketamin (10mg/kg) and xylazine (2mg/kg) intramuscularly. Screening test using tuberculin skin test (TST) method (old mammalian tuberculin kit) was carried out to all samples, 0.1 mL (135000 Tuberculin Unit) on palpebrae intradermally. The effect of TST was examined at 24, 48, and 72 hours post treatment. The test was repeated on the second week. The data were tabulated and analyzed descriptively. From the identification of macaca populations in two areas was found one group of  macaca  in Kilometer Nol (N 05° 54? 19,1" E 95° 12? 57,4")  with the population of 14 macacas whereas in Cot Murong area (N 05° 54? 19,1" E 95° 12? 57,4") found two groups with the population of 12 and 14 macacas. TB screening test using TST method showed that there were no incidents of TB in long tail macaque in Sabang tourism area.Keywords : Tuberculin Skin Test, Sabang, Macaca fascicularis  
UJI DAYA HAMBAT EKSTRAK BIJI BUAH PALA (Myristica fragrans Houtt) TERHADAP PERTUMBUHAN BAKTERI Escherichia coli (TEST OF INHUBITORY POWER OF NUTMEG SEEDS (Myristica fragrans Houtt) EXTRCT ON GROWTH OF BACTERIA Escherichia coli) Syarifah, Raysa; Fakhrurrazi, Fakhrurrazi; Harris, Abdul; Sutriana, Amalia; Erina, Erina; Winaruddin, Winaruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.16 KB) | DOI: 10.21157/jim vet..v2i3.8203

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ABSTRAKPenelitian ini bertujuan untuk mengetahui daya hambat ekstrak biji buah pala (Myristicafragrans Houtt) terhadap pertumbuhan bakteri Escherichia coli. Biji pala yang digunakan dalam penelitian ini diperoleh dari pasar tradisional Darussalam, Banda Aceh. Proses ekstrasi biji buah pala menggunakan pelarut Etanol 96% dengan tingkat konsentrasi yang berbeda yaitu 1%, 10%,20%.  Menggunakan kertas cakram kosong (Blank disk) yang telah dicelupkan ke dalam ekstrak biji buah pala masing-masing pengulangan dan antibiotik ampisilin yang diletakkan pada media MHA. Pengujian daya hambat dilakukan dengan metode difusi Kirby-Bauer menggunakan kertas cakram pada media Mueller hinton agar (MHA) dengan 5 perlakuan dan 3 pengulangan. Media agar diinkubasi pada suhu 36-37ºC selama 24 jam. Hasil penelitian ini menunjukkan bahwa bahwa ektsrak  biji  buah  pala  memiliki  daya  hambat  yang  lemah  terhadap  pertumbuhan  bakteri Escherichia coli.  Ekstrak biji buah pala pada konsentrasi 1% memiliki daya hambat sebesar 9,73 mm, konsentrasi 10% sebesar 8,5 mm, konsentrasi 20% sebesar 8 mm dan kontrol positif sebesar20,83 mm.Kata kunci : antibakteri, ekstrak biji buah pala, Escherichia coli.. ABSTRACTThis study aims to determine the inhibitory power of nutmeg extract (Myristica fragransHoutt) on the growth of Escherichia coli bacteria. The nutmeg seed used in this study was obtained from the traditional market of Darussalam, Banda Aceh. Extraction process of nutmeg seed using96% ethanol solvent with different concentration level that is 1%, 10%, 20%. Using blank disc paper (Blank disk) which has been dipped into nutmeg extract of each repeating and ampicillin antibiotic placed on MHA media. Inhibitory testing was performed by the Kirby-Bauer diffusion method using paper discs on Mueller hinton agar medium (MHA) with 5 treatments and 3 repetitions. Media to be incubated at 36-37ºC for 24 hours. The results of this study indicate that the nutmeg nut extraction has a weak inhibitory effect on the growth of Escherichia coli bacteria. The extract  of nutmeg  seed  at concentration  of 1%  has inhibitory power  of 9.73  mm,  10%concentration of 8.5 mm, 20% concentration of 8 mm and positive control of 20.83 mm.Keywords : antibacterial, nutmeg ekstact, Escherichia coli.