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Pencemaran Bakteri dalam Air Sumur di Sekitar Peternakan Sapi Potong di Yogyakarta Suwito, Widodo; Supriadi, .; Winarti, Erna; Tisnawati, Nyoman Ayu Anggreni
Acta VETERINARIA Indonesiana Vol. 2 No. 2 (2014): Juli 2014
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (717.371 KB) | DOI: 10.29244/avi.2.2.43-48

Abstract

Air sumur merupakan salah satu sumber air untuk keperluan rumah tangga. Tujuan dari penelitian ini adalah untuk mengetahui kualitas mikrobiologi air sumur di sekitar kandang kelompok sapi potong di Yogyakarta. Telah dikumpulkan sebanyak 12 contoh air sumur di sekitar kandang kelompok sapi potong dari Kabupaten Sleman, Kulon Progo, dan Bantul. Contoh air sumur diperiksa terhadap Coliform dan E. coli dengan metode most probable number (MPN), sedangkan Salmonella sp. dengan isolasi dan identifikasi dengan metode Andrews & Hammack. Sebanyak 91,6% dari 12 contoh air sumur, jumlah Coliform dan E. coli melebihi ambang batas baku mutu air rumah tangga. Salmonella sp. berhasil diisolasi dari air sumur di sekitar kandang sapi potong Kabupaten Kulon Progo. Dari hasil penelitian dapat disimpulkan bahwa air sumur di sekitar kandang di Yogyakarta hampir seluruhnya tercemar Coliform dan E. Coli.Kata kunci: pencemaran, air sumur, peternakan, sapi potong. (Contamination of Bacteria in Well Water Around Beef Cattle Farm in Yogyakarta)Well water is one of the sources of water to use in housewifery. The aim of this study was to determine microbiological contamination of well water around beef cattle farms in Yogyakarta. A total of 12 well water samples were collected from around beef cattle farms in Sleman, Kulon Progo, and Bantul district. These samples were analyzed for Coliform and E. coli by using most probable number (MPN), where as Salmonella sp. with isolation and identification by Andrews & Hammack methods. A total 91.6% of 12 well water samples have Coliform and E. coli that exceeds the threshold household water quality standards. Salmonella sp. was isolated from well water around beef cattle farm in Kulon Progo district. In conclusion, the well water samples around beef cattle farms in Yogyakarta contaminated Coliform and E. coli.Keywords: contamination, well water, livestock, beef cattle.
ISOLASI DAN KARAKTERISASI Staphylococcus aureus DARI SUSU KAMBING DAN PRODUK OLAHANNYA Suwito, Widodo; Winarti, Erna; Widyastuti, Ari; Kristiyanti, Felisitas; Andriani, Andriani
Jurnal Teknologi dan Industri Pangan Vol. 28 No. 1 (2017): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.303 KB) | DOI: 10.6066/jtip.2017.28.1.85

Abstract

Presence of Staphylococcus aureus in goat milk and dairy product could lead to human illness. The aim of the present study was to characterize S. aureus isolated from goat milk and its products. The samples used in these studies were taken from goat farm and a goat milk processing facility in Sleman district. Characterization of the S. aureus was based on biochemical reaction, namely haemolysin activity, clumping factor, coagulase activity, and resistance against antibiotics. The haemolysin activity was determined by culturing the isolates on blood agar plates, whereas clumping factor with slide agglutination test. Mixing the rabbit plasma and culture S. aureus was used to determine the coagulase activity, while antibiotics susceptibility was carried out with agar diffusion test. The resulst showed that the number of S. aureus detected in 86% of goat milk samples conformed with SNI No 01-6366-2000. The characteristics of S. aureus from goat milk samples showed that 80% of the S. aureus isolates were non haemolytic, 20% were positive for clumping factor, and 40% were positive for coagulase activity. The antibiotic resistance test for S. aureus isolated from the goat milk samples suggested that 30% was resistant to ampicillin and penicillin while 10% showed resistance to erythromycin, neomycin, sulfonamide, and tetracycline.
Isolasi and Identification of Bacteria from the Urine Fluid Organic Fertilizer (POC)Ettawa Crossbred (PE) in The Sleman Regency Suwito, Widodo; Wahyuni, A.E.T.H.; Nugroho, Widagdo Sri; Sumiarto, Bambang; Bekti, Utomo Bimo
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.569 KB) | DOI: 10.22146/jsv.3781

Abstract

Urine is a by product from Ettawa crossbred (PE) goat farm in Sleman regency. In addition, the manure urine goats can be used for fluid organic fertilizer (POC). Recently, organic farming is more interesting for people because it does not use any chemical fertilizers. Salmonella sp and E. coli O157:H7 are pathogenic bacteria that can contaminate agricultural products and dangerous for public health. One of sources of contamination in agricultural products is due to the organic fertilizer. Therefore, the aim of the present study was to determine pathogenic bacteria from POC urine PE goats in Sleman regency. A total of 8 POC samples were collected from PE goat urine in Sleman regency. These samples were analyzed for Salmonella sp, E. coli O157:H7 and other pathogenic bacteria by using biochemical reactions. All samples are negative for Salmonella sp. and E. coli O157:H7 as well. Whereas, 85% of 8 samples are positive for Pseudomonas sp. The present study showed that POC from PE goats farm in Sleman regency are negative for pathogenic bacteria.  
Determinasi Salmonella sp dan Ektoparasit dalam Pupuk Organik dari Kotoran Sapi Potong di Yogyakarta Suwito, Widodo; -, Supriadi; Winarti, Erna; Bimo Bekti, Utomo
Biota Biota Volume 13 Nomor 2 Tahun 2014
Publisher : PBI Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.19 KB)

Abstract

AbstractOne of by products derived from beef cattle farm is manure. The manure can be processed to make organic fertilizer. Salmonella sp is bacterium of the manure origin and harmful for human. Currently, organic fertilizer is mostly used for vegetables and fruits, but the problem is people consume those vegetables. The aim of this study was to determine Salmonella sp and ectoparasite in organic fertilizer which were made from the manure of beef cattle farm in Yogyakarta. A total of 10 manure samples were collected the farms to make organic fertilizer. The manure was fermented with lactic acid bacteria (BAL) for one month. Before and after fermented, the manure was isolated and identified for Salmonella sp based on biochemical reactions and ectoparasite using native method. The study showed that there were no Salmonella sp and ectoparasite in the organic fertilizer made from the manure fermented for one month.Keywords: Organic fertilizer, manure, farm, Salmonella spAbstrakSalah satu hasil sampingan dari peternakan sapi potong adalah kotoran ternak. Kotoran ternak dapat dimanfaatkan untuk dibuat pupuk organik. Salmonella sp merupakan bakteri yang berasal dari kotoran ternak dan dapat membahayakan kesehatan manusia. Saat ini pupuk organik banyak digunakan untuk tanaman sayuran dan buah-buahan, sedangkan sebagian masyarakat mengonsumsi sayuran dalam keadaan mentah. Tujuan dari penelitian ini adalah untuk mengetahui Salmonella sp dan ektoparasit dalam pupuk organik yang dibuat dari kotoran sapi pada peternakan sapi potong di Yogyakarta. Telah dikumpulkan sebanyak 10 sampel kotoran sapi yang akan dibuat pupuk organik dari peternakan sapi potong di Yogyakarta. Kotoran sapi difermentasi dengan bakteri asam laktat (BAL) selama satu bulan. Kotoran sapi sebelum dan sesudah difermentasi dilakukan isolasi dan identifikasi Salmonella sp berdasarkan reaksi biokimia dan ektoparasit dengan metode natif. Penelitian ini menunjukkan bahwa di dalam kotoran sapi yang dibuat pupuk organik dengan fermentasi selama satu bulan tidak ditemukan Salmonella sp.Kata kunci: Pupuk organik, rabuk, peternakan sapi, Salmonella sp
Impacts of Verotoxigenic and Enterohaemorrhagic Escherichia Coli (Vtec and Ehec) on Animal, Human and Food Suwito, Widodo
Indonesian Bulletin of Animal and Veterinary Sciences Vol 19, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.554 KB) | DOI: 10.14334/wartazoa.v19i2.922

Abstract

Verotoxigenic and enterohaemorrhagic Escherichia coli, VTEC and EHEC are strains that produce a toxin on the Vero cells in vitro, therefore the toxin called verotoxin. Strain VTEC and EHEC have been isolated from human and various animal species, mainly ruminants and pigs. The prevalence of VTEC in cattle is 35%, milk 10%, cheese 1.5%. In beef meat, pork, poultry, goat and sheep is 3.7, 1.5, 1.5, 2 and 2.5%, respectively. In contrast, the human prevalence in United States, Spain and France 5 – 10, 2.5 and 3.4%, respectively. In Indonesia, nine cases of VTEC were reported by Ciptomangunkusumo Hospital and four out of nine cases were died. The verotoxin also named Shiga toxins (Stx), is active in vivo as a pathogen on the endothelial cells of the blood vessels of the gastro intestinal mucosa, kidneys, brain and other tissues of human and piglets. Verotoxin in human, ruminants and piglets causes bloody diarrhea so with ruminants. In the adult ruminants, verotoxin results in clinical signs and they act as asymptomatic carriers of VTEC and EHEC strains but in human, it produced haemorrhagic colitis (HC), haemolytic uremic syndrome (HUS) and thrombocytopenia purpura (TPP). Infection in human by the most famous EHEC strain belongs to the O157:H7 serotype, through faecal contamination or environment of either food of animal origin, or other foodstuffs (fruits and vegetables). Infection from EHEC strains could be prevented by avoiding food of animal origin and unpasteurized milk, and by taking care of food hygiene for comsumption.   Key words: verotoxin, enterohaemorrhagic, VTEC, EHEC, Shiga toxin (stx), ruminant
Mastitis in Ettawa Crossbred Goat (Pe) Caused by Staphylococcus Aureus: Epidemiology, Clinical Signs, Pathogenesis, Diagnosis and Control Suwito, Widodo; S, Indarjulianto
Indonesian Bulletin of Animal and Veterinary Sciences Vol 23, No 1 (2013)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.598 KB) | DOI: 10.14334/wartazoa.v23i1.953

Abstract

Mastitis in Ettawa crossbred goat is commonly found and caused economic loss. Staphylococcus aureus is one of bacteria caused clinical mastitis or subclinical mastitis in Ettawa crossbred goat. The aim of this review was to provide an overview of clinical and subclinical mastitis in the Ettawa crossbred goat caused by S. aureus from epidemiological aspect, clinical symptoms, pathogenesis, diagnosis, treatment, prevention and control. Mastitis should be eliminated because it lead to death for the goats and lambs. In addition, S. aureus has greater risk for contamination in milk because it produces heat-stable toxin. Isolation and identification bacteria with total of somatic cell counts are important as a reference to determine the actions to decrease the occurrence of mastitis. Some preventive measures for mastitis include clean milking, dipping the teats with a disinfectant and antibiotic treatment during dry lactation. Key words: Ettawa crossbred goat, mastitis, S. aureus, diagnosis, preventif
Verotoxigenic Escherichia coli (VTEC) isolated from cow milk Suwito, Widodo
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.713 KB) | DOI: 10.14334/jitv.v14i3.346

Abstract

Verotoxigenic Escherichia coli (VTEC) strains are responsible for serious human illnesses. These strains are commonly found in milk. The aim of this study was to determine the occurrence of verotoxigenic E. coli in milk. A total of 351 milk samples, were collected from dairy farms in Bogor, Sukabumi and Cianjur. These samples were analyzed for VTEC using biochemical, serological and vero cell cytotoxicity assays. VTEC O157:H7 isolates were found in milk collected from dairy herds in Bogor and Sukabumi at rates 0.47% of 214 samples, 1.10% of 91 samples respectively, and none in Cianjur. Hemolytic E. coli isolates were found in 0.94% of 214 milk samples from Bogor, 2.2% of 91 milk samples from Sukabumi and none from Cianjur. From E. coli isolates, 53 isolates (67.95%) were verotoxigenic, consisted of: two E. coli O157:H7 isolates and 51 non O157:H7 isolates.Therefore this study showed the occurrence of VTEC in milk samples from dairy farms in Bogor, Sukabumi and Cianjur. Key words: Milk, E. coli (VTEC) O157:H7, Verotoxigenic
ANALISIS MIKROBIOLOGI SUSU KAMBING PERANAKAN ETTAWA (PE) DARI KABUPATEN SLEMAN YOGYAKARTA Suwito, Widodo; Sri Nugroho, Widagdo; Wahyuni, AETH Wahyuni; Sumiarto, Bambang
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.189 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2625

Abstract

Tujuan penelitian ini mengetahui kualitas mikrobiologi susu kambing mentah yang diambil langsung dari ambing. Dalam penelitian ini digunakan 50 sampel susu kambing peranakan Ettawa (PE). Sampel dianalisis terhadap total plate count (TPC), jumlah Staphylococcus sp., jumlah koliform, Escherichia coli (E. coli), dan Salmonella sp. berdasarkan reaksi biokimia. Rerata untuk TPC; Staphylococcus sp.; total koliform masing-masing adalah 1,65x10 3 ; 5,75x10 3 ; 1,3x10 cfu/ml, sedangkan E. coli dan Salmonella sp. adalah negatif. Berdasarkan Standar Nasional Indonesia (SNI) No 01-6366-2000 tentang persyaratan susu segar, maka TPC, koliform, E. coli, dan Salmonella sp. memenuhi standar, sedangkan Staphylococcus sp. melebihi ambang batas. Hasil penelitian menunjukkan bahwa susu kambing mentah yang diambil langsung dari ambing masih layak konsumsi.
DETEKSI Escherichia coli 0157:H7 DARI SUSU DAN DAGING MENGGUNAKAN SERUM KEBAL MONOSPESIFIK Suwito, Widodo
Jurnal Sain Veteriner Vol 28, No 2 (2010): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2951.202 KB) | DOI: 10.22146/jsv.295

Abstract

Escherichia coli 0157:H7 merupakan salah satu bakteri patogen penyebab food borne disease pada manusia. Deteksi E.coli 0157:H7 pada serum keba] monospesifik berdasarkan somatik antigen (0) dan flagella(H). Tujuan dari penelitian ini untuk mendeteksi E.coli 0157:H7 menggunakan serum kebal monospesifik dengan cara absorbsi dan uji koaglutinasi. Serum kebal monospesifik diperoleh dengan cara menyuntikkan antigen E.coli 0] 57:H7 pada kelinci jenis New Zealand White. Serum dikoleksi dan diabsorbsi menggunakan antigen somatik E.coli heterolog sampai tidak terjadi reaksi agglutinasi. Hasil penelitian menunjukkan bahwa dengan serum kebal monospesifik dapat digunakan untuk menedeteksi E.coli 0 157:H7 dalam susu dan daging.Kata kunci: serum keba1, monospesifik, E.coli 0157:H7
Mastitis in Ettawa Crossbred Goat (Pe) Caused by Staphylococcus Aureus: Epidemiology, Clinical Signs, Pathogenesis, Diagnosis and Control Suwito, Widodo; S, Indarjulianto
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 23, No 1 (2013): MARCH 2013
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.598 KB) | DOI: 10.14334/wartazoa.v23i1.953

Abstract

Mastitis in Ettawa crossbred goat is commonly found and caused economic loss. Staphylococcus aureus is one of bacteria caused clinical mastitis or subclinical mastitis in Ettawa crossbred goat. The aim of this review was to provide an overview of clinical and subclinical mastitis in the Ettawa crossbred goat caused by S. aureus from epidemiological aspect, clinical symptoms, pathogenesis, diagnosis, treatment, prevention and control. Mastitis should be eliminated because it lead to death for the goats and lambs. In addition, S. aureus has greater risk for contamination in milk because it produces heat-stable toxin. Isolation and identification bacteria with total of somatic cell counts are important as a reference to determine the actions to decrease the occurrence of mastitis. Some preventive measures for mastitis include clean milking, dipping the teats with a disinfectant and antibiotic treatment during dry lactation. Key words: Ettawa crossbred goat, mastitis, S. aureus, diagnosis, preventif