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Journal : Jurnal Pengolahan Hasil Perikanan Indonesia

POTENSI KOLAGEN TERIPANG EMAS SEBAGAI INHIBITOR TIROSINASE Safithri, Mega; Setyaningsih, Iriani; Tarman, Kustiariyah; Yuhendri, Vitriyanna Mutiara; Meydia, Meydia
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 21 No. 2 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.598 KB) | DOI: 10.17844/jphpi.v21i2.23085

Abstract

Kolagen memiliki aktivitas antioksidan dan mampu menghambat aktivitas tirosinase pada prosesmelanogenesis, salah satu biota laut yang dapat dijadikan sumber kolagen alternatif adalah teripang.Penelitian ini bertujuan menentukan karakteristik kolagen dan aktivitas penghambatan enzim tirosinaseoleh kolagen teripang emas (Stichopus hermanii). Penghilangan protein non kolagen dilakukan dengancara perendaman daging teripang menggunakan NaOH 0,1 M selama 48 jam. Isolasi kolagen dilakukanmenggunakan asam asetat 0.5 M selama 48 jam. Analisis gugus fungsi kolagen dilakukan menggunakanFTIR dan analisis aktivitas tirosinase dilakukan menggunakan spektrofotometer. Kadar protein nonkolagen yang diperoleh yaitu 0,090 mg/mL dan kolagen teripang emas memiliki rendemen 0,66%. Kolagenteripang memiliki nilai pH 6,91, gugus fungsi khas amida A (3379,29 cm-1), amida B (2924,09 cm-1), amidaI (1681,93 cm-1), amida II (1568,13 cm-1), dan amida III (1269,16 cm-1). Aktivitas penghambatan tirosinasemenunjukkan bahwa kolagen teripang emas memiliki nilai IC50 5610 ppm. Kolagen teripang emas belummampu menghambat aktivitas tirosinase.
PROFILE OF AMINO ACID, FATTY ACID, AND MINERAL CONTENT OF TAMBELO (BACTRONOPHORUS SP.) FROM KENDARI, SOUTHEAST SULAWESI Riviani, Riviani; Purwaningsih, Sri; Tarman, Kustiariyah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 19 No. 1 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.295 KB) | DOI: 10.17844/jphpi.v19i1.11696

Abstract

Public coastal had trust natural material as medicine became one of the most important things inthe discovery of medicine latest. Papua, Belitung, and Kendari coastal communities believed that tambelo(Bactronophorus sp.) can treat various kinds of diseases like lumbago, rheumatism, cough, flu, malaria, andimprove production of the breast milk, increase appetite, and vitality of man. It was important to know whatcourse content of tambelo. Tambelo could be examined amino acids, fatty acids, and mineral contents. Thehighest essential amino acids in tambelo were leucyne, lysine, and valin of 0.57%, 0.39%, 0.36%. The highestnon essential amino acid was alanin, glutamic acid, and aspartic acid of 1.24%, 1.09%, and 0.78%. Barrierof amino acid was histidine. Fatty acid total of tambelo was 29.52%, whereas the fatty acid compositionsconsist of 10.09% saturated fatty acid (SAFA) was 14.37% monounsaturated fatty acid (MUFA) was 5.06%polyunsaturated fatty acids (PUFAs). Among them, those occuring in the highest proportions were palmaticacid (4.49%), oleic acid (5.73%), palmitoleic acid (4.96%), and Aracidic acid (1.88%). Tambelo had n6/n3ratio of 1.84. Tambelo had mineral contents as Natrium of 1144000 mg/kg, calcium of 17000 mg/kg, Kaliumof 21000 mg/kg, magnesium of 13000 mg/kg, phosphor of 1900 mg/kg, cadmium < 0.24 mg/kg, and lead<1.25 mg/kg.Keyword : amino acids, Bactronophorus sp., fatty acids, mineral contents, tambelo
ANTICANCER ACTIVITY OF SPIRULINA CULTIVATED IN WALNE AND ORGANIC MEDIA Sirait, Putriana Sari; Setyaningsih, Iriani; Tarman, Kustiariyah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 1 (2019): Jurnal Pengolahan Hasil Perikanan
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.088 KB) | DOI: 10.17844/jphpi.v22i1.25876

Abstract

Spirulina is Cyanobacteria containing active components which is potentially showing anticancer activity. The purposes of this study were to determine anticancer activity and selectivity of crude extractsof Spirulina cultured using Walne and organic media, and to detect the apoptosis. The stages of this study included cultivation and harvesting of Spirulina, active components extraction, anticancer test and apoptosisdetection. Anticancer activity was determined using MTT assay. The crude extracts of Spirulina from Walne and organic cultures contained active components of alkaloids, flavonoids, steroids, and saponins. Theseextracts were not toxic to normal breast cells (MCF-12a), but showed cytotoxic activity in breast cancer cells (MCF-7). The crude extract of Spirulina from Walne culture had IC50 value of 36.23 ppm and selectivity index 30.07, while the IC50 of organic culture was 117.78 ppm and selectivity index 7.17. Detection of apoptosis with Hoechst dye 33342 showed the apoptotic activity of Spirulina crude extract against MCF-7 cells.
KARAKTERISTIK FISIKOKIMIA KOLAGEN LARUT ASAM DARI KULIT IKAN PARANG-PARANG (CHIROCENTRUS DORAB) Safithri, Mega; Tarman, Kustiariyah; Suptijah, Pipih; Widowati, Neni
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 22 No. 3 (2019): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.307 KB) | DOI: 10.17844/jphpi.v22i3.28924

Abstract

Waste of parang parang fish (Chirocentrus dorab) skin can be used as a source of collagen. Collagen isolation can be done chemically by the Acid Soluble Collagen (ASC) method. The objective of this research was to isolate collagen with ASC method and characterize their physicochemical. Collagen isolation consisted of pretreatment and hydrolysis with acids. The pretreatment used NaOH 0.1 M for 12 hours, while hydrolysis used acetic acid 0.5 M. Pretreatment results indicated that the concentration of non-collagen protein was 0.1243 mg/mL, while the yield collagen was 2.61%. The collagen had the viscosity of 6.50 cP, the denaturation temperature of 4°C, the transition temperature of 77.30°C, and the melting temperature of 153.90°C. The obtained collagen also had pH of 6.25. The fourier transform infrared (FTIR) spectra analysis showed the collagen contained amide A (3425.58), B (2924.09), I (1647.21), II (1543.05), and III (1246.02) (cm-1). The collagen also contained glycine (26.69%), proline (12.24%) and alanine (9.51%).
CHARACTERIZATION AND PHOTOPROTECTOR ACTIVITY OF ENDOPHYTIC FUNGAL PIGMENTS FROM COASTAL PLANT SARANG SEMUT (HYDNOPHYTUM FORMICARUM) Sibero, Mada Triandala; Tarman, Kustiariyah; Hanif, Novriyandi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 19 No. 1 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.119 KB) | DOI: 10.17844/jphpi.v19i1.12512

Abstract

Endophytic fungus RS3 isolated from coastal plant sarang semut (Hydnophytum formicarum) produced extracellular black pigment. The aims of this research were to obtain the pigment, to characterize and to determine the photoprotector activity. This research was conducted into several steps, that were determination of the best precipitating agent, characterization using instrument and solubility analysis, and analysis of Sun Protection Factor (SPF). Results showed the pigment was precipitated using acid solvent with pH ? 2.5. Functional groups of pigment were hydroxyl, aromatic ring, phenol and amine. According to its characteristics, black pigment produced by RS3 isolate was proposed as melanin. The photoprotector analysis showed the SPF value was 11.33.
AKTIVITAS ANTIBAKTERI EKSTRAK KAPANG LAUT NODULISPORIUM SP. KT29 TERHADAP VIBRIO HARVEYI Hariati, Sri; Wahjuningrum, Dinamella; Yuhana, Munti; Tarman, Kustiariyah; Effendi, Irzal; Saputra, Fazril
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 21 No. 2 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (363.422 KB) | DOI: 10.17844/jphpi.v21i2.22855

Abstract

Nodulisporium sp. KT29 merupakan salah satu kapang endofit yang diisolasi dari alga merah Eucheuma edule, tidak bersifat toksik, dan bersifat antibakteri. Penelitian ini bertujuan mengevaluasi aktivitas antibakteri ekstrak kapang laut Nodulisporium sp. KT29 terhadap Vibrio harveyi. Penelitian dilakukan dengan beberapa tahapan yaitu kultivasi kapang, ekstraksi senyawa aktif, pengujian aktivitas antibakteri, kromatografi lapis tipis (KLT), bioautografi, serta pengamatan kerusakan sel bakteri Vibrio harveyi. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etil asetat dan dipekatkan dengan rotary evaporator. Ekstrak Nodulisporium sp. KT29 diuji terhadap bakteri Vibrio harveyi pada konsentrasi 0,125; 0,25; 0,5; 1 dan 2 mg/disc. Ekstrak Nodulisporium sp. KT29 membentuk zona hambat tertinggi pada konsentrasi 2 mg sebesar 45,33±0,71 mm. Hasil uji KLT ekstrak Nodulisporium sp. KT29 menunjukkan adanya 7 spot retardation factor (Rf) dengan tiga komponen warna yaitu cokelat, kuning dan ungu. Uji bioautografi menghasilkan 2 spot zona hambat yaitu 16 mm dan 13 mm pada Rf 0,94 dan 0,14. Pengamatan menggunakan SEM menunjukkan bahwa kerusakan pada morfologi sel Vibrio harveyi dengan pemberian ekstrak Nodulisporium sp. KT29 yang ditandai dengan lisis.
PRODUKSI PIGMEN DAN IDENTIFIKASI KAPANG PENGHASILNYA MENGGUNAKAN PENDEKATAN DNA BARCODING Sibero, Mada Triandala; Tarman, Kustiariyah; Radjasa, Ocky Karna; Sabdono, Agus; Trianto, Agus; Bachtiarini, Tiara Ulfa
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 21 No. 1 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(1)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (531.122 KB) | DOI: 10.17844/jphpi.v21i1.21454

Abstract

Kapang endofit laut diketahui sebagai sumber berbagai senyawa bioaktif yang berguna bagi kesehatan. Penelitian yang telah dilakukan sebelumnya membuktikan bahwa kapang endofit isolat RS3 asal tanaman pesisir Hydnophytum formicarum asal Sorong menghasilkan pigmen hitam dengan potensi sebagai fotoprotektor namun pada penelitian tersebut tidak memiliki data mengenai produksi pigmen serta identifikasi kapang penghasilnya. Penelitian ini bertujuan untuk menentukan produksi pigmen hitam asal kapang RS3 dan identifikasi kapang RS3 melalui pendekatan DNA barcoding. Kapang RS3 dikultivasi selama 30 hari menggunakan sistem statis dan sistem shaking. DNA barcoding pada identifikasi kapangRS3 menggunakan primer universal ITS1 dan ITS4 untuk mengamplifikasi wilayah internal transcribed spacer (ITS) yang menjadi conserve region pada kingdom fungi. Hasil penelitian menunjukkan bahwa kapang RS3 yang dikultivasi menggunakan sistem shaking mulai memproduksi pigmen pada hari-6 dan mencapai produksi tertinggi pada hari ke-18 (2,42±0,03 mg/L) sedangkan pada kultivasi sistem statis rendemen pigmen terbesar dihasilkan pada hari ke-30 (2,88±0,21 mg/L). Berdasarkan hasil analsis ITS rDNA diketahui bahwa kapang endofit RS3 memiliki 99% kemiripan dengan Annulohypoxylon stygium. Kapang ini telah didaftarkan di GeneBank denga nomer akses  MG605083.1.
KANDUNGAN KOMPONEN BIOAKTIF DAN AKTIVITAS ANTIMIKROB EKSTRAK BINTANG LAUT (CULCITA SCHMIDELIANA) Tarman, Kustiariyah; Prestisia, Hana Nurullita; Setyaningsih, Iriani; Meydia, Meydia; Yogiara, Yogiara; Hwang, Jae-Kwan
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 15 No. 3 (2012): Jurnal Pengolahan Hasil Perikanan Indonesia 15 (3)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.925 KB) | DOI: 10.17844/jphpi.v15i3.21418

Abstract

Bintang laut Culcita schmideliana merupakan salah satu spesies dari kelas Asteroidea yang memiliki potensi sebagai antimikrob. Tujuan penelitian ini adalah untuk menentukan komponen bioaktif dan aktivitas antimikrob dari bintang laut C. schemideliana. Penelitian ini dilakukan melalui beberapa uji, yaitu uji komponen fitokimia, uji aktivitas antimikrob, dan fraksinasi dengan kromatografi lapis tipis, serta bioautografi. Hasil uji fitokimia secara kualitatif menunjukkan bahwa ekstrak metanol mengandung alkaloid, steroid, flavonoid, saponin, dan ninhidrin, sedangkan ekstrak n-heksan dan etil asetat mengandung steroid dan saponin. Aktivitas antimikrob terbesar ditunjukkan oleh ekstrak etil asetat dengan konsentrasi 2 mg yaitu sebesar 16 mm. Fraksinasi KLT diidentifikasi memiliki 7 bercak pada lampu sinar UV ? 254 nm dan UV ? 366 nm menghasilkan 1 bercak. Pengamatan bioautografi terhadap bakteri B. subtilis menghasilkan zona hambat sebesar 7 mm pada Rf = 0,02 dan pada Rf = 0,62 menghasilkan zona hambat sebesar 3 mm.
ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF GREEN ALGAE HALIMEDA GRACILIS FROM SERIBU ISLAND DISTRICT Basir, Abdul; Tarman, Kustiariyah; Desniar, D.
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 20 No. 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (404.455 KB) | DOI: 10.17844/jphpi.v20i2.17507

Abstract

Seaweeds have ecological functions as primary producers in marine waters. It also has an important economic value as a producer of hydrocolloids (alginate, agar and carrageenan) that is used in various industries of food and pharmaceuticals. This study aimed to determine the antibacterial and antioxidant activity of green algae Halimeda gracilis. The study was conducted in several stages, sample collection and preparation, extraction of bioactive compound, fractionation, antibacterial and antioxidant test, and phytochemical. Extraction was done by maceration method using methanol and concentrated by rotary evaporator. The methanol extracts of H. gracilis were tested against Staphylococcus aureus and Escherichia coli. Methanol extract of H. gracilis formed inhibition zone against the test bacteria with diameter of inhibition zone was 10 mm and 6 mm, respectively. After liquid-liquid partition (water: ethyl acetate), inhibition zone was only seen in the ethyl acetate fraction of H. gracilis with diameter of inhibition zone was 6 mm and 7.50±1.71 mm, respectively. Antioxidant test methanol extracts and ethyl acetate fractions of H. gracilis each show IC50 value of 290.49 ppm and 375.50 ppm. Phytochemical test showed methanol extract of H. gracilis contains phenols and steroids.
ANTICANCER ACTIVITY FROM ACTIVE FRACTION OF SEA CUCUMBER Putram, Nurul Mutia; Setyaningsih, Iriani; Tarman, Kustiariyah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 20 No. 1 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1191.863 KB) | DOI: 10.17844/jphpi.v20i1.16399

Abstract

Sea Cucumber Holothuria atra is one of marine organisms has been used as a new source of novel bioactive compounds. Many of them have been used as the lead compounds in discovery of new anticancer drugs. The objective of this study was to determine the active fractions of sea cucumber (H. atra) which have anticancer activity. H. atra was macerated using ethanol and the extract was freezedried using a freeze dryer. The crude extract was partitioned using n-hexane, ethyl acetate, and methanol-water (3:1:1:1). Cytotoxicity test was performed using HeLa (cervic cancer) cell line and MCF-7 (breast cancer) cell line based on the MTT assay. The crude extract of H. atra showed the best cytotoxic activity against HeLa cells (IC50 = 12.48 µg/mL) and MCF-7 cells (IC50 = 17.90 µg/mL). The toxicity tests showed the IC50 value of the n-hexane fraction, ethyl acetate fraction, and methanol-water fraction against HeLa cells HeLa (IC50 = 76.45 µg/mL; 77.95 µg/mL;  14.27 µg/mL) and MCF-7 cells (IC50 = 58.50 µg/mL; 59.59 µg/mL; 14.33 µg/mL).