Risa Tiuria
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Bogor Agricultural University (IPB)

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EVALUASI PIRIPROKSIFEN DALAM OVITRAP UNTUK MENGENDALIKAN NYAMUK AEDES SPP. PADA SKALA SEMI LAPANG (SEMIFIELD SCALE EVALUATIONS OF PYRIPROXYFEN IN OVITRAP FOR CONTROL AEDES SPP. MOSQUITOES) Karmila, Mila; Hadi, Upik Kesumawati; Tiuria, Risa
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2019.20.4.471

Abstract

Ovitrap is an alternative mosquito control method that used to reduce the population of Aedes spp, vector of dengue hemorrhagic fever. This study aims to evaluate pyriproxyfen in ovitrap to control Aedes spp through the mechanism of autodissemination by these mosquitoes on a semifield scale. The research was carried out from August to October 2018 in the outdoor environment of Braja Mustika Bogor Hotel. Ovitrap used consist of two types, namely In2Care traps the which contained insecticide of pyriproxyfen, Beauveria bassiana and yeast, (2) the standar ovitraps (without insecticide). In2Care trap (30 pieces) was installed with a distance of 400 m2/trap, while the standard ovitrap (10 pieces) with a distance of 4 m randomly around In2Care trap. Observations were conducted once a week for two months. The parameter observed in In2Care trap was the number of larvae and pupae Aedes spp, which live and dead. The observations on standard ovitrap were the presence of Aedes spp eggs, the number of larvae pupae and adults that  eclosed. The results showed that the In2Care traps effectively attracted Aedes spp to laid eggs and breed (86.7-100.0%). The number of larvae observed in In2Care trap ranged from 10-50 larvae (33.380.0%) and all larvae that developed in In2care trap (100%) died after being kept in the laboratory for two months. The results of observations on standard ovitraps showed that there were dead pupae (22.7-80.8%) and adults that failed eclotion (22.6-83.6%) during 2 months of observation. The results showed that there was the effective mechanism of pyriproxyfen autodissemination by Aedes spp from In2Care traps to standard ovitraps when laying their eggs. 
ENDOPARASIT CACING PADA ORANGUTAN EX-CAPTIVE DI SUAKA MARGASATWA SUNGAI LAMANDAU KALIMANTAN TENGAH INDONESIA Mirsageri, M.; Assidiqi, M. Jamaluddin; Cahyaningsih, Umi; Tiuria, Risa; Z, Zulfiqri
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2796

Abstract

Penelitian ini bertujuan melakukan identifikasi dan mengetahui persentase orangutan yang terinfeksi endoparasit (cacing) pada orangutan excaptive yang berada di Suaka Margasatwa Sungai Lamandau. Sampel feses orangutan yang diperiksa sebanyak 30 individu yang didapatkan dicamp Gemini dan Siswoyo. Pemeriksaan feses dilakukan secara makroskopis dan mikroskopis. Pemeriksaan mikroskopis dilakukan dengan menggunakan dua metode yaitu metode Ridley dengan larutan sodium acetate, acetic acid, dan formaldehyde (SAF) fiksatif dan metode apung. Hasil pengamatan menunjukkan adanya infeksi cacing gastrointestinal sebesar 56,7%. Hasil penelitian menunjukkan bahwa orangutan positif terinfestasi telur cacing tipe ascarid; trichurid; dan strongiloid masing-masing sebesar 43,3; 16,7; dan 6,7%. Orangutan dapat mengalami infeksi lebih dari satu jenis tipe telur cacing.
GOBLET CELLS PROLIFERATION OF DUODENUM, JEJUNUM, AND ILEUM OF LAYING HENS IMMUNIZED WITH PROTEIN OF EXCRETORY-SECRETORY OF ASCARIDIA GALLI Balqis, Ummu; Tiuria, Risa; Priosoeryanto, Bambang Pontjo; D, Darmawi
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3129

Abstract

This research was conducted in order to examine the goblet cells proliferation in duodenum, jejunum, and ileum of laying hens due to exposured with protein of excretory/secretory (ES) of Ascaridiagalli adult worm. Thirty heads of laying hens were divided in to two groups. The first group was treated with 4,000 infective larva (L2) of A. galli and the second group was immunized with 380µg of ES andfour hours later was challenged with 4000 L2. All treatments were given orally using stainless steelcanule directly to the oesophagus. Data was taken on the 3, 6, 9, 12, and 15 days post immunization(p.i.). The goblet cells were determined by Periodic Acid Schiff (PAS) staining. The result showed that immunization was able to increased goblet cells proliferation significantly at 12 and 15 day p.i. on theduodenum, and at 9, 12, and 15 day p.i. on the jejunum, but goblet cells proliferation did notsignificantly on the ileum. From this result we suggested that ES would beneficial in the strengthen thehost?s defence mechanisms in the intestinal mucosa.Keywords: Ascaridia galli, excretory/secretory, goblet cells
THE EFFECT OF EXCRETORY/SECRETORY PRODUCT RELEASED BY L3 OF A. GALLI ON VILLOUS COMPACT IN INTESTINE OF LAYING HENS Balqis, Ummu; Darmawi, .; Tiuria, Risa; Priosoeryanto, Bambang P.
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

The nematode, Ascaridia galli, caused pathological changes in the gut, while the larval development takes place in the intestine epithelium. The aim of this research was to examine the effect of excretory/secretory product released by L3 of A. galli to prevent intestine destructions based on villous compact in duodenum, jejunum, and ileum of laying hens. Excretory/secretory was prepared from metabolic of L3 released in culture médium in flasks containingrosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Excretory/secretory product was concentrated with vivaspin 30.000 MWCO. The chickens vaccinated with 80 g protein excretory/secretory in crudemixed with Fruend Adjuvant Complete and repeated three times with dose of each 60 g mixed with Freund Adjuvant Incomplete with an interval of one week intra muscularly. The chickens were challenged with 1000 infective larvae A. galli one week later. After two weeks the chickens were operated. Villous compact in duodenum, jejunum, and ileum of laying hens were analyzed. The result showed that immunization was able to sustained significantly intestinevillus compact, particularly in duodenum. This research concluded that the excretory/secretory product could protect villus in duodenum against parasitic diseases caused by A. galli.
THE DEVELOPMENT OF ASCARIDIA GALLI INFECTIVE EGGS BY IN VITRO CULTURE Balqis, Ummu; D, Darmawi; Hambal, Muhammad; Tiuria, Risa
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i2.3104

Abstract

The aim of this study was to determine the survival of embrionated eggs of Ascaridia galli. Adult female worms were obtained from lumen of intestine of native chickens in a slaughter house. Eggs obtained from the uteri of adult female worms were incubated in distilled water at room temperature for 20-31 days in order to develop A. galli infective eggs. The eggs were counted using stereomicroscope. The result showed that the amount of A. galli eggs were 1,045,478 and the amount of embrionated eggs were 935,300 (89.46%).Keywords: Ascaridia galli,  embrionated eggs
POPULASI ASCARIDIA GALLI DALAM USUS HALUS AYAM YANG DIBERIKAN KOMBINASI EKSKRETORI/SEKRETORI L3 DAN IMUNOGLOBULIN YOLK Darmawi, Darmawi; Balqis, Ummu; Tiuria, Risa
Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v11i2.370

Abstract

Ascaridia galli populations in intestine of chickens treated with combination of excretory/secretory L3 and immunoglobulin yolkABSTRACT. The purpose of the present study was to determine the presence of worm populations in intestine of chickens vaccinated and combined with egg yolk to experimental Ascaridia galli infection. Amount of 18 head chickens were devided into six groups (A ? F). Group A, the chickens were not vaccinated. Group B, the chickens were vaccinated with excretory/secretory of A. galli L3. Group C, the chickens were vaccinated with excretory/secretory of A. galli L3, challenged with dose 1000 L2, and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group D, the chickens were vaccinated with excretory/secretory of A. galli L3 and challenged with dose 1000 L2. Group E, the chickens were challenged with dose 1000 L2 and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group E, the chickens were challenged with dose 1000 L2. Intestinal worm burdens of infected groups were recorded. The result showed that excretory/secretory of A. galli L3 combined with egg yolk decreased significantly A. galli survival in intestine of laying hens. Vaccinations were positively correlated with worm burden at 12 weeks after chalanged. The results suggest that A. galli L3 excretory/secretory product contain potential antigen and that antibody-mediated mechanisms contribute to immune protection.
AN ESTIMATE OF MEAT PRODUCTION LOSS IN NATIVE CHICKEN IN BOGOR AND ITS SURROUNDING DISTRICTS DUE TO GASTEROINTESTINAL HELMINTHIASIS He, Simon; Susilowati, Vici E.H.S.; Purwati, Endang; Tiuria, Risa
Hemera Zoa Vol. 74 No. 3 (1991): Jurnal Hemera Zoa
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.264 KB)

Abstract

Among 78 native chicken from Bogor and its surrounding districts, only  4 (5.1%) with mean dressing weights of 1250 ± 29 g were free from parasiric worms. The other 74 chicken found infected with nematodes [mean nematode numbers (mn) = 24]. Cestodes (mc = 45) and trematodes (mt = 7). Single parasite class infections of nematode (mn = 18) and of trematode (mt = 96) were each one sample (1.3% with mws 1250 and 1180 g, respectively); single parasite class infections of cestode (mc = 47) were 15 (19.2% wit mw = 1145 g). Total single parasite class infections were 17 (21.8%). Mixed nermatode (mn = 29) + castode (mc = 50) were 41 (52,6%; mw = 1056 g), nematode (mn = 4) + trematode (mt = 28) were 2 (2.6% mw = 1225 g) and cestode (mc = 44) + trematode (mt = 6 - were 2 (2,6%; ws = 975 g). Total two parasite class infections were 45 (57.7%). Mixed infections of nematode (mn - 45) + cestode (mc = 44) + trematode (mt = 27) were 12 (15,4%; mw = 1034 g).
L3 POPULATIONS IN LAYING HENS INFECTED WITH 6,000 L2 OF ASCARIDIA GALLI D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedono, Retno D.; Pasaribu, Fachriyan H.
Jurnal Kedokteran Hewan Vol 1, No 2 (2007): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i2.3122

Abstract

The aim of the present study was to determine the survival of L3 populations in intestine ofchickens exposed to experimental Ascaridia galli infection. Nature female adult worm were obtained fromlumen of village chickens in a comercial abattoir in Bogor. The eggs (L1) obtained from uteri female adultworms were incubated in sterile aquadestilata at room temperature for 10-20 days developed embrionatedeggs (L2). Five groups (A-D) of 80 head chickens were infected with, 6000 L2 A. galli respectively. Thechickens of group A were infected six times with dose of each 1,000 L2 with an interval of one hour. Thechickens of group B were infected three times with dose of each 2,000 L2 with an interval of two hours.The chickens of group C were infected six times with dose of each 3,000 L2 with an interval of three hours. The chickens of group D were infected one time with single dose 6,000 L2. A. galli L3 were recovered from intestines of 80 heads chickens seven days after oesophagus inoculation with 6,000 L2.The result showed that total 702,000 L1 and 628,000 L2 collected from 124 A. galli female adult worms.The percentage of L1 developed L2 is 89.46% and L2 developed L3 is 11.27%. Significant survival of L3higher populations in intestine of chickens observed only in the group D. The results indicated thatchickens infected high dose of A. galli caused the decrease of host defence against ascaridiosis. Keywords: Ascaridia galli, embrionated eggs, larvae
RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF ASCARIDIA GALLI D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedoeno, Retno Damayanti; Pasaribu, Fachriyan Hasymi; Hambal, Muhammad; Daud, Razali
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.929

Abstract

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P 0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.
SEROPREVALENSI TRICHINELLOSIS PADA BABI HUTAN DI KABUPATEN BENGKULU TENGAH, PROVINSI BENGKULU Lestari, Milda; Satrija, Fadjar; Tiuria, Risa
Jurnal Ilmu Pertanian Indonesia Vol. 23 No. 3 (2018): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.987 KB) | DOI: 10.18343/jipi.23.3.220

Abstract

Trichinellosis is a zoonotic disease caused by nematode of the genus Trichinella infection. Transmission of this disease in humans occurs because of eating meat, especially pork containing Trichinella spp. larvae in raw or undercooked conditions. Data on trichinellosis cases in humans and animals in Indonesia are still very limited. This study was carried out to identify the seroprevalence of Trichinella spp. in wild boar in Central Bengkulu District and the level of public knowledge about trichinellosis. Sampling method was used cross sectional method by taking blood serum sample from captured wild boar at TPH in Talang Boseng of Bengkulu Province. Result of serological test using indirect ELISA showed seroprevalence of trichinellosis was 68.2% of total 44 samples obtained. Seropositive results based on the sub-district of the wild boar captured were 12 of 18 (66.7%) in Pondok Kubang and 18 of 26 (69.2%) in Pondok Kelapa. All wild boar meat consumer respondents did not understand that consumption of wild boar meat could become the source of trichinellosis transmission.