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PRODUKSI ANTIBODI ANTI-DIROFILARIA IMMITIS UNTUK PENGEMBANGAN DIAGNOSIS DIROFILARIASIS PADA ANJING Erawan, I Gusti Made Krisna; Tjahajati, Ida; Nurcahyo, Wisnu; Asmara, Widya
Buletin Veteriner Udayana Vol. 8 No. 2 Agustus 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Dirofilaria immitis (D. immitis) sebagai agen penyebab penyakit cacing jantung tidak hanya menimbulkan masalah pada hewan tetapi juga bersifat zoonosis. Untuk mendiagnosis dirofilariasis (penyakit yang disebabkan oleh D. immitis) secara serologis dibutuhkan antibodi anti-D. immitis.  Penelitian ini bertujuan untuk memproduksi antibodi terhadap antigen ekskretori-sekretori cacing jantan dan cacing betina untuk pengembangan diagnosis berbasis deteksi antigen. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa antigen ekskretori-sekretori jantan (male excretory-secretory antigens/MES), antigen ekskretori-sekretori betina (female excretory-secretory antigens FES) dan antigen ekskretori-sekretori jantan dicampur dengan betina (MES+FES) D. immitis dapat merangsang pembentukan antibodi poliklonal pada mencit BALB/c dengan pola produksi yang sama. Antibodi telah terbentuk pada hari ke-21 dan titernya mencapai puncak pada hari ke-35 setelah imunisasi.
THE EFFICACY OF DORAMECTIN FOR TREATMENT OF ANCYLOSTOMIASIS IN DOGS Tjahajati, Ida
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8241

Abstract

A study was done to evaluate the efficacy of doramectin injection for Ancylostomiasis treatment in 10 dogs. All of the dogs were heavily and naturally infected with Ancylostoma Sp. the heavy infection criterion (25 egg per gram) was based on the flotation method. At the beginning of the study the dogs were injected doramection subcutaneously (200 ,wg/kg of body weight). The pathogenesis of the disease was observd through the clinical sign and feses examination during 2 weeks after treatment. Dogs were considered recovery when they have a good response to the treatment and clinically showed normal of apetite, no sign of diarrhea and no egg was found laboratorically. Results of the study showed that all of the dogs had positive response to the treatment and therefore it can be concluded that doramectin effective against Ancylostoma Sp. in dogs. 
CIMETID1NE ENHIBITS ELIMINAT1ON OF SULPHAMETHOXAZOLE IN RABBIT {ORYCTOLAGUS CUNICULUS) Tjahajati, Ida
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8247

Abstract

Cimetidine was reported to inhibit elimination many other drugs. The research has been done to study the effect of cimetidine on elimination of sulphamethoxazole in rabbits. Twenty healthy male rabbits, 10 months of age were used as experimental animals. The rabbits were allotted into 4 groups of 5 each Group I as a control group were intramuscularly injected with 25 mg sulphamethoxazole. Group 11 were intramuscularly injected 25 sutphamethoxazole and pretreated with 12.5 mg cimetidine 30 minutes before injection. Group 111 were intramuscularly injected 25 mg sulphamethoxazole, pretreatod with 25 mg cimetidine 30 minutes before injection. Group IV were intramuscularly injected 25 mg sulphamethoxazole, pretreated with 50 mg cimetidine 30 minutes before injection. Blood sample were collecled at 0.25, 0 75, 1,75, .1.75, 10 and 24 hours after injection. Plasma sulphamethoxazole levels were determined using the Bratton-Marshall method Elimination kinetic parameters were analyzed statistically using anava. The result of the study showed that cimetidine significantly reduced total clearance of sulphamethoxazole <P<0.l). Based on the result it can be concluded that cimetidine inhibits elimination of sulphamethoxazole.
ISOLATION, IDENTIFICATION AND SENSITIVITY TEST OF BACTERIA FROM DIARRHEA CASES IN DOG AT THE VETERINARY CLINIC, FACULTY OF VETERINARY MEDICINE, GADJAH MADA UNIVERSITY Yuriadi, Yuriadi; Tjahajati, Ida
Jurnal Sain Veteriner Vol 16, No 2 (1999)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8602

Abstract

A study was conducted to find out bacteria which probably play an important role in causing diarrhea in dog at the veterinary clinic Gadjah Mada University. Sample were collected from diarrhea cases of dog, during the periode of September to December 1997, by rectal swabbing. Samples were then sent to the laboratory for bacterial isolation and identification.The result indicated that E. coli was found from 50% of the sample, Streptococcus sp 41.9%, Staphylococcus sp 33.3% of sample, Enterobacter sp 16.7%, Bacillus sp 8.3%.Some of E. coli isolates were pathogenic to mice. The pathogenic E. coli were resistant to penicillin, ampicillin, streptomycin, erythromycin, doxycycline, besides sensitive to neomycin, gentamycin, kanamycin, polymyxin B, chloram-phenicol and sulfisoksale.
THE EFFECT OF FEVER ON ACETYLATION RATIO AND SULFADIAZINE ELEMINATION KINETICS OF LOCAL SHEEP (OVIS ARIES) Tjahajati, Ida; Prodjoharjono, Subronto; Santoso, Budiono
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8621

Abstract

A Study has been conducted to identify effect of fever towards acetylafion ratio and eleminatkm kinetics of sulfadiazine in sheep. The siudy consist 10 healthy sheep using cross­over design. Each animal was treated twice with 40 days interval. In the first treatment the sheep were given 2 gram of sulfadiazine and 0.4 gram pyrimethamine. In the second treatment the animals were treated fever induction prior to treatment using 2 gram of sulfadiazin and 0.4 gram pyrimethamine. Blood samples were taken From jugular vein in hour of 0, 0.08.0.25, 0.5, 1,2,4,6,9.12, 18, and 24. Plasma sulfadiazine levels were analyzed using Bratton-Marshall Methode. Sulfadiazin parameter was calculated using Stripe program. Theresults of the study revealed that fever did not significantly (P>0,05) increased the aceiylation ratio and inhibited elemination of sulfadiazine.
IDENTIFIKASI SEROVAR PENYEBAB LEPTOSPIROSIS PADA ANJING DI YOGYAKARTA Mulyani, Guntari Titik; Hartati, Sri; Wuryastuty, Hastari; Tjahajati, Ida; Yuriadi, Yuriadi; Widiyono, Irkham; Yanuartono, Yanuartono; Purnamaningsih, Hary; Indarjulianto, Soedarmanto; Raharjo, Slamet; Nururozi, Alfariza; Ganapragasam, Angeline; Jiao, Yeo Suan
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.014 KB) | DOI: 10.22146/jsv.39201

Abstract

Leptospirosis is a zoonotic disease of global concern, and is caused by pathogenic serovar Leptospira interrogans. Canine Leptospirososis is widespread worldwide, dogs can act as incidental hosts or maintenance hosts for various serovars. The purpose of this research was to identify leptospire serovars that infect healthy and suspected leptospirosis dogs in Yogyakarta. A total of 56 dogs (36 healthy dogs and 20 suspect leptospirosis dogs) sera were taken from cephalica vein as much as 3 ml. Sera were examined for leptospirosis with Microscopic Aglutination Test (MAT) which conducted at the Research Center for Veterinary Science, Bogor. Microscopic Aglutination Test carried out on various Leptospire serovar, namely: Ichterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Australis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, and Tarrasovi. The results showed that Celledoni serovars infected 25% of healthy dogs and 5% of suspect leptospirosis dogs, Javanica serovar infected 19% of healthy dogs, Bataviae serovars infected 15% of suspect leptospirosis dogs, Grippotyphosa serovar infected 11% of healthy dogs, Tarrasovi serovar infected 10% of suspect leptospirosis dogs, serovars Cynopteri infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, serovar Pyrogenes infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, and serovar Rachmati infects 5% of suspect leptospirosis dogs. Seven healthy dogs (19%) and 2 suspect leptospirosis dogs (10%) were infected with more than 2 leptospire serovars. From the results of this study it can be concluded that Celledoni serovar of Leptospira interrogans infection causes subclinical leptospirosis, while Bataviae serovar infection causes clinical leptospirosis in dogs in Yogyakarta.
Reactive Oxygen Intermediate (ROI) in Dog Macrophage Infected with Mycobacterium tuberculosis Tjahajati, Ida
Indonesian Journal of Biotechnology Vol 11, No 2 (2006)
Publisher : Universitas Gadjah Mada

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Abstract

experiment used 24 healthy dogs, aged between 1 and 2 years, both male and female which were divided into twodifferent groups consisting of 12 dogs each. The first group was the treatment group, that is they were infected with Mtuberculosis and the second one was the control group. The activity of macrophages ROI secretion were measured at1st, 2nd, 12th, and 24th after infection using nitroblue tetrazolium (NBT) reduction assay. Three cats were used to measure themacrophage activity in each period, using triplicate sample for each cat. The results of the experiment showed thatROI secretion increased in infected group compared with the control group, and this activity reached to the plateaulevel at 2 weeks after infection. Although these enhanced activities were gradually diminished thereafter, higherlevels of these activities were consistently observed until the end of experiment compared with control group. Theresults of the experiment indicated that ROI played an important role to against M.tuberculosis infection in dogs.Keyword: macrophage, ROI, M.tuberculosis, dogs
SA-2 REPRESENTATION OF VARIOUS SYSTEMATIC DISRUPTIONS WITHIN THE PATIENTS HANDLED AT “KLINIK HEWAN JOGJA” BETWEEN JANUARY AND JUNE 2018 Tjahajati, Ida; Chrissandra, Adellyna; Prayitno, Anggi D; Aditya, Begum F R; Rrarindah, E; Rifiyanta, L; Candra, M A; Widiastuti, T A; Astuti, S I
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Various diseases or disruption could affect all of the patients handled at any clinic or hospital. The health problems would be varied, depending on the disease or systemic disruption that ailed the patient. This research aims to figure out the spread of said health problems in the patients handled at Klinik Hewan Jogja between January and June 2018.A numerical representation of afflictions that ails the patients in a clinic or a hospital during a period of time would provide the information needed to anticipate several diseases, facilitate easier treatment and follow-up actions for certain ailments, offer prevention methods, and lastly to yield data for clients' education. The data gathered is also vital to give a general picture the problems that frequent a certain area, thus imparting important information to all other clinics or hospitals in the said region.
AKTIVITAS SEKRESI REACTIVE OXYGEN INTERMEDIATE (ROI) PADA MAKROFAG PERITONEUM KUCING YANG DIINFEKSI DENGAN M.TUBERCULOSIS Tjahajati, Ida; Prodjoharjono, Subronto; Subono, Hardyanto; Asmara, Widya; Harada, Nobuyuki
Jurnal Sain Veteriner Vol 22, No 1 (2004): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2796.721 KB) | DOI: 10.22146/jsv.444

Abstract

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VAKSINASI BCG MENINGKATKAN AKTIVITAS FAGOSITOSIS DAN SEKRESI REACTIVE OXYGEN INTERMEDIATE (ROI) PADA MAKROFAG PERITONEUM KUCING YANG DIINFEKSI DENGAN MYCOBACTERIUM TUBERCULOSIS Tjahajati, Ida
Jurnal Kedokteran Brawijaya Vol 21, No 2 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (971.59 KB) | DOI: 10.21776/ub.jkb.2005.021.02.3

Abstract

ABSTRACT The aim of this experiment was to study the effect of BCG vaccination on phagocyte activity and ROI secretion in cat peritoneum macrophages which infected with M.tuberculosis. The experiment used twenty four healthy cats. The animals were divided in 2 groups, 12 cats in each group. Group I were vaccinated with BCG, group II were control group which unvaccinated. BCG vaccination was done twice in two weeks interval. Two days after vaccination each cat was infected by 105 cfu M.tuberculosis intraperitoneally. The activity of macrophages were measured  at 1st, 2nd, 12th, and 24th, after infection using in vitro latex bead phagocyte and NBT reduction assay. Three cats were used to measure the macrophage activity in each period, using triplicate sample for each cat. The results of the experiment showed that the phagocyte activity and ROI secretion increased significantly in vaccination group (P&lt;0.01) compared with the control group, and these activities reached to the plateau level at 2 weeks after infection. Although these enhanced activities were gradually diminished thereafter, higher levels of these activities were consistently observed until the end of experiment compared with control group. BCG vaccination increased the cellular immunity especially phagocyte and ROI secretion activities of peritoneual macrophages in cat infected with M.tuberculosis Key words: BCG, phagocyte, ROI, M.tuberculosis, cat