Galuh Tresnani
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

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THE USE OF FISH FLOUR AND PALM SUGAR MEDIUM TO GROW MATARAM INDIGENOUS ISOLATE OF ENTOMOPATHOGENIC BACILLUS THURINGIENSIS FOR CONTROLLING AEDES AGYPTI LARVAE Suryadi, Bambang Fajar; Hidayat, Hilal Aisyah; Tresnani, Galuh
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.446 KB) | DOI: 10.11594/jtls.09.02.04

Abstract

To grow Mataram indigenous local isolate of entomopathonic Bacillus thuringiensis using 4 combinations of fish flour and palm sugar natural liquid medium. The culture was then tested against 3rd instar Aedes aegypti larvae to gain lethal concentration (LC) value within 72-hour test. Four combinations of fish flour and palm sugar liquid medium (in 1:1; 1:3; 1:5 and 1:7 weight ratio) were made to grow B. thuringiensis isolate Bt-TP2B. Cell, endospore and protein crystal were recorded every 24 hours within 72-hour incubation. Seventy-two-hour-old B. thuringiensis cultures from those media were serially diluted, then tested against 3rd instar B. thuringiensis larvae. Larval death was recorded every 24 hours within 72-hour observation to obtain lethal concentration (LC) values. Results from all medium were compared to those of NYSM standar medium. B. thuringiensis isolate BT-TP2B could grow in fish flour and palm sugar natural medium. The highest toxicity (the lowest LC) was seen on B. thuringiensis grown on fish flour and palm sugar natural medium in 1:1 weight ratio. The LC50 values were 1.98x103 cell/mL (in 24-hour observation) and 1.49x103 cell/mL (in 48-hour and 72-hour observation), while LC90 values were 1.35x103 cell/mL in 24-hour observation and 1.58x103 cell/mL in 48 and 72-hour observations). These values were lower (more toxic) compared to those of NYSM standard medium. Fish flour and palm sugar natural medium could be used to grow entomopathogenic B. thuringiensis. Moreover, this natural medium could increase toxicity of B. thuringiensis against B. thuringiensis3rd instar larvae. 
PENYULUHAN UPAYA PENANGGULANGAN DAN PEMERIKSAAN CACINGAN SEBAGAI IMPLEMENTASI PROGRAM PESANTREN SEHAT Taisir, Atwazzah; Hapsari, Ria; Pratama, Iman Surya; Aini, Siti Rahmatul; Tresnani, Galuh; Suryadi, Bambang Fajar
Transformasi: Jurnal Pengabdian Masyarakat Vol 15 No 2 (2019): Transformasi Desember
Publisher : LP2M Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.533 KB) | DOI: 10.20414/transformasi.v15i2.1789

Abstract

[Bahasa]: Angka prevalensi cacingan nasional pada tahun 2015 sebesar 28,12%, dengan prevalensi daerah yang bervariasi hingga melebihi 50%. Survey cacingan pada sampel anak SD/MI di Kabupaten Lombok Barat pada tahun 2011 menunjukkan angka prevalensi 29,47%. Cakupan upaya penanggulangan yang masih terbatas mendorong peningkatan koordinasi lintas mitra salah satunya pesantren. Program pengabdian kepada masyarakat ini bertujuan untuk mengimplementasikan upaya penanggulangan cacingan melalui penyuluhan dan demonstrasi indikator Perilaku Hidup Bersih dan Sehat (PHBS) pada salah satu mitra pondok pesantren di daerah Lombok Barat. Metode pengumpulan data yang digunakan yaitu wawancara semi terstruktur dan observasi pengetahuan siswa dengan lembar kuisioner. Berdasarkan pemeriksaan natif terhadap 38 sampel feses terdapat 3 orang cacingan (0.7 %)  terdiri atas infeksi campuran cacing tularan tanah (Ascaris sp dan Trichuris sp) dan infeksi tunggal (Hymenolepis sp, Ascaris sp). Hasil pengabdian secara umum berkontribusi terhadap penguasaan indikator PHBS sederhana yang terdiri atas: Cuci Tangan Pakai Sabun (CTPS), penggunaan alas kaki, serta pengukuran berat, dan tinggi badan secara berkala. Kata Kunci: cacingan; pesantren sehat; PHBS [English]: The national worm prevalence rate in 2015 was 28.12%, with regional prevalence varies over 50%. Worms survey in a sample of elementary school children in West Lombok Regency in 2011 showed a prevalence rate of 29.47%. The limited scope of prevention efforts has led to increased coordination across partners including pesantren. This community service program aimed to implement the prevention of intestinal worms through counseling and demonstration of clean and healthy behaviors (PHBS) indicators at one of the boarding schools in West Lombok. The methods used in this program is semi-structured interview and observation of students? knowledge with questionnaire sheets. Based on the native examination of 38 feces samples, there were three students who have worms (0.7%) consisting of mixed infection of earthworm (Ascaris sp and Trichuris sp) and single infection (Hymenolepis sp, Ascaris sp). Generally, this program contributes to the mastery of simple PHBS indicators consisting of: handwashing with soap (CTPS), footwear use, and periodic weight and height measurements. Keywords: worm infestation; healthy boarding school; personal hygiene
PROFIL PROTEIN STADIUM SPOROZOIT EIMERIA TENELLA ISOLAT YOGYAKARTA MELALUI ANALISIS PROTEIN SDS-PAGE (PROTEIN PROFILE OF THE SPOROZOITE OF EIMERIA TENELLA ISOLATES FROM YOGYAKARTA USING SDS-PAGE PROTEIN ANALYSIS) Tresnani, Galuh; Prastowo, Joko; Nurcahyo, Wisnu; Daryono, Budi Setiadi
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Coccidiosis is one of the important diseases in poultry industry. In Indonesia the morbidity of thedisease is between 80 to 90%. A rapid and prompt diagnosis would be one of the essential steps ineradication and control of the disease. The objective of this study is to determine the protein profile ofsporozoite of Eimeria tenella isolated in Yogyakarta using sodium dodecyl sulphate polyacrilamide gelelectrophoresis (SDS-PAGE) protein analysis. Protein analysis was performed in 12% polyacrilamide geland further electrophoresis at 100 volts and over-staining with Coomasie brilliant blue. The resultsshowed that the sporozoite of E. tenella isolated in Yogyakarta contained five proteins with molecularweights of 15, 26, 32, 80, and 91 kDa, respectively.
PENGEMBANGAN ANTIBODI POLIKLONAL DARI STADIUM OOSISTA, SPOROSISTA, DAN SPOROZOIT EIMERIA TENELLA (THE DEVELOPMENT OF POLYCLONAL ANTOBODY FROM EIMERIA TENELLA OOCYST, SPOROCYST, AND SPOROZOITE STADIUM) Tresnani, Galuh; Prastowo, Joko; Nurcahyo, Wisnu; Daryono, Budi Setiadi
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The research on developing diagnostic method, vaccine, and drugs for coccidiosis has been focused onthe finding of the immunogenic molecule in Eimeria. The identification of this agent will need the antibodywhich can recognize the biomolecule in the antigen. Antibody that has been developed for this purposeshould be analyzed first, and one of the simple methods for analyzing this antibody is through dot blotanalysis. The objective of this research was to analyze the polyclonal antibody which developed from theoocyst, sporocyst, and sporozoite of  E. tenella using dot blot analysis. The antigen for this polyclonalantibody was made from each of the E. tenella stadium by sonication. Fifteen mice, divided into 3 groups,were then injected subcutaneously with each antigen. The sera from these mice were then collected, analyzedby using ELISA and then it will be used for the dot blot analysis. The research result showed that thepolyclonal antibody which has been developed in mice from each antigen can react with the antigen itself.From this result it can be concluded that the developing of this antibody is successful and it can be used forfurther research in immunoproteomic.