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THE HISTOLOGICAL, EXTRACTION AND CHARACTERIZATION COLLAGENS YELLOW-PIKE CONGER MUARENESOX TALABON Gadi, Dewi Setiyowati; Trilaksani, Wini; Nurhayati, Tati
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v9i2.19300

Abstract

By product Muarenesox talabon swim bladders can be used as a raw material for desperately needed in the food, biomedical, pharmaceutical, and cosmeceuticals industries. The aims of the research were to observed the histological and determine chemical characteristics of swim bladder including proximate and amino acids; extraction of acid soluble collagen and determine the characteristics of collagen including proximate, pH, heavy metals, microbial, amino acids, functional groups, molecular weights, and thermal stability. The morphology of cunang swim bladder consists of outer, middle, and inner layers containing collagen fibers; 33.67±0.71%wb and protein whichwere dominated by three amino acids that were glycine, proline, and alanine. Pretreatment by 0.1 M NaOH for 8 hours (K1T4) and acid extraction by 0.25 M acetic acid for 72 hours (M1T3) was the best treatment yielding 14.51± 0.43% of collagen; having 12.12±0.04% wb of moisture; 88.54 ± 0.08% wb of protein; 1.31±0.23% wb of fat; 0.17±0.03% wb of ash. Not detected any heavy metals (Pb, Hg, As, Cd). Acidity pH was 4.31 and negative of E. coli and Salmonella. The main amino acids detected were glycine 241.06 mg/g; proline 88.73mg/g; and alanine 86,98 mg/g; FTIR spectra were revealed the presence of triple helix structures; electrophoresis patterns consisted of 136 kDa of mol weight of ?1 and 117 kDa of mol weight of ?2 were characterisedto be type I collagen; which had Tmax of 195.59ºC and ?H 7.8113 J/g. Keywords: acid extraction, swim bladder, collagens, thermal stability
CHARACTERIZATION OF NANOHYDROXYAPATITE FROM TUNA’S THUNNUS SP BONE AS BIOMATERIALS SUBSTANCE Hanura, Aditya Bayu; Trilaksani, Wini; Suptijah, Pipih
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v9i2.19296

Abstract

Tuna Thunnus sp is a high-value fisheries products. The abundance of waste from tuna?s processing industry has not been used optimally. The objective of this study was to produce and characterize nanohidroxyapatite (Ca5(PO4)3((OH)0.8F0.2) of tuna?s Thunnus sp bone waste using high temperature sintering of 400-800OC continuing by nanomilling method. The results of the characterization showed that the sintering temperature of 800OC was the best treatment yielding of hidroxyapatite 57.73%, 89.27% white degree level. The result of statistic analysis showed that the sintering temperature variation gave a significant effect on the white degree at the test level (p <0.05). Proximate result after sintering showed moisture content of 0.15%, 99.83% ash, 0.05% fat, 0.73% protein and 0% crude fiber. Results of heavy metal analysis showed the content of lead (Pb) and mercury (Hg) was negative, and cadmium (Cd) 0.018 ppm. The mineral test consist of calcium, phosphor, kalium, magnesium, iron, zinc, manganese, and sodium with the dominant elements were 49.76% calcium and 9.02% phosphor. The result of statistic analysis showed that the sintering temperature variation gave significant effect to the three main minerals at the test level (p<0.05). The Particle Size Analyzer test showed that nanohidroxyapatite particles was measured at 800-900 nm. The X-Ray Diffractometer analysis showed the results of sample identification contains hydroxyapatite and fluorian, with syn 100.00%. The hidroxyapatite was a compound complex and crystals formed has a hexagonal shape with a percentage of 82.9% crystallinity. Keywords : bone, nanohidroxyapatite, particles, sintering, tuna
CHARACTERIZATION OF FUNCTIONAL PROPERTIES FISH PROTEIN CONCENTRATE OF SKIPJACK ROE (KATSUWONUS PELAMIS) Rieuwpassa, Frets Jonas; Santoso, Joko; Santoso, Joko; Trilaksani, Wini; Trilaksani, Wini
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 2 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.962 KB) | DOI: 10.29244/jitkt.v5i2.7559

Abstract

By product that rich in protein such as fish roes are potential as raw material for protein concentrate. This research aimed to utilize skipjack roes to produce protein concentrate and to characterize its functional properties. The method used to extract protein was defatting method using isopropyl alcohol and ethanol with extraction times of 1, 2, and 3 hours.  The results showed that skipjack roes contained 19.81% of protein, 3.41% of fat, 71.32% of moisture, 2.04% of ash, and 1.53% of carbohydrate (by difference). Defatting method using isopropyl alcohol for 3 hours produced the best roe protein concentrate (RPC).  The product meets to the quality requirements of fish protein concentrate type B, contained protein and fat of 71.79% and 2.78%, respectively. This product also had functional properties as follows: water absorption capacity (1.57 ml/g), oil absorption capacity (1.82 g/g), emulsion capacity (81.65%), bulk density (0.51 g/ml), foaming capacity (1.90 ml), foaming stability (0.22 ml) and protein digestibility (95.86%).  Lysine and leucine became the major essential amino acid of RPC, with values were 70.76 and 64.91 mg/g protein, respectively. The composition of amino acids of RPC skipjack consisted of 8 essentials amino acids, 5 non-essentials amino acids and 2 semi-essentials amino acids. Keywords: extraction, fish roe skipjack, roe protein concentrate
CHARACTERIZATION OF FUNCTIONAL PROPERTIES FISH PROTEIN CONCENTRATE OF SKIPJACK ROE (KATSUWONUS PELAMIS) Rieuwpassa, Frets Jonas; Santoso, Joko; Santoso, Joko; Trilaksani, Wini; Trilaksani, Wini
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 5 No. 2 (2013): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.962 KB) | DOI: 10.29244/jitkt.v5i2.7559

Abstract

By product that rich in protein such as fish roes are potential as raw material for protein concentrate. This research aimed to utilize skipjack roes to produce protein concentrate and to characterize its functional properties. The method used to extract protein was defatting method using isopropyl alcohol and ethanol with extraction times of 1, 2, and 3 hours.  The results showed that skipjack roes contained 19.81% of protein, 3.41% of fat, 71.32% of moisture, 2.04% of ash, and 1.53% of carbohydrate (by difference). Defatting method using isopropyl alcohol for 3 hours produced the best roe protein concentrate (RPC).  The product meets to the quality requirements of fish protein concentrate type B, contained protein and fat of 71.79% and 2.78%, respectively. This product also had functional properties as follows: water absorption capacity (1.57 ml/g), oil absorption capacity (1.82 g/g), emulsion capacity (81.65%), bulk density (0.51 g/ml), foaming capacity (1.90 ml), foaming stability (0.22 ml) and protein digestibility (95.86%).  Lysine and leucine became the major essential amino acid of RPC, with values were 70.76 and 64.91 mg/g protein, respectively. The composition of amino acids of RPC skipjack consisted of 8 essentials amino acids, 5 non-essentials amino acids and 2 semi-essentials amino acids. Keywords: extraction, fish roe skipjack, roe protein concentrate
KAJIAN KERUSAKAN TABLET HISAP SPIRULINA SELAMA PENYIMPANAN Setyaningsih, Iriani; Trilaksani, Wini; Desniar, .; Masruroh, Emma; Fahleny, Ria; Gentini, Vatin Tri
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.62 KB)

Abstract

Spirulina is a microalgae have a high nutrient content and bioactive compound were good for health. This microalgae can be cultivated indoor or outdoor as needed. One of the applications of Spirulina is for lozenges. The purpose of this study is determine the effect of packaging and shelf life of Spirulina lozenges on the growth of bacteria and fungi. This microalgae can be cultivated indoor or outdoor as needed. One of the applications of Spirulina is for lozenges. Bacteria and fungi still grow on the Spirulina lozenge which packaged using alumunium foil and plastic bottle. However bacteria and fungi total on Spirulina lozenge during storage on 8 weeks are not different.
PENGARUH DEFATTING, FREKUENSI PENCUCIAN DAN JENIS DRYOPROTECTANT TERHADAP MUTU TEPUNG SURIMI IKAN LELE KERING BEKU [EFFECT OF DEFATTING, WASHING CYCLE AND DRYOPROTECTANT TYPE ON THE QUALITY OF FREEZE DRIED CATFISH SURIMI POWDER] Ramadhan, Wahyu; Santoso, Joko; Trilaksani, Wini
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (850.569 KB) | DOI: 10.6066/jtip.2014.25.1.47

Abstract

PENGARUH DEFATTING, FREKUENSI PENCUCIAN DAN JENIS DRYOPROTECTANT TERHADAP MUTU TEPUNG SURIMI IKAN LELE KERING BEKU[Effect of Defatting, Washing Cycle and Dryoprotectant Type on the Quality of Freeze Dried Catfish Surimi Powder]Wahyu Ramadhan*, Joko Santoso dan Wini Trilaksani Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor Diterima 05 Agustus 2013 / Disetujui 08 April 2014ABSTRACT  Freeze dried surimi powder is a surimi type processed by freeze drying. To utilize oversized catfish and to reduce surimi handling cost, oversized catfish has been used as raw material of surimi powder. The study aimed to determine the effect of defatting (NaHCO3 concentration and soaking duration), washingcycle,and dryoprotectant type on catfish surimi, as well as quality differences between surimi powder and wet surimi. With regard to defatting step, soaking in NaHCO3 0.75% for 10 minutes was found as the best treatment and resulting in a fat content of 1.52%. Moreover, one time of washing cycle was found as the most appropriate procedure to obtain a superior quality of surimi with whiteness value 57.21%, water holding capacity 73.28%, salts soluble protein 7.17%, pH 6.69, and gel strength 482.3 g/cm2, folding value of 4.84, and teeth cutting value of 8.26. Trehalose 6% was the most suitable dryoprotectant resulting in surimi powder with water holding capacity of 8.01 mL/g, gel strength 826.3 g/cm2, salt soluble protein 18.98%, density 4.06 mL/10 g, rehydration capacity 3.81, emulsion capacity 69.3%, emulsion stability 59.3%, foaming capacity 25.33% and foaming stability 9.40%. The microstructure profile of surimi powder added with trehalose had more compact tissues, without any damage and clots, than that treated with other dryoprotectants. However, surimi powder still had lower protein content than wet surimi, and lower physical and chemical properties, particularly in its teeth cutting and folding characteristics.  
MANFAAT PENERAPAN STANDAR PADA PERUSAHAAN TUNA DI DKI JAKARTA Linthin, Retno Dwiputri; Zakaria, Fransiska R; Trilaksani, Wini
MANAJEMEN IKM: Jurnal Manajemen Pengembangan Industri Kecil Menengah Vol. 13 No. 1 (2018): Manajemen IKM
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.92 KB) | DOI: 10.29244/mikm.13.1.39-45

Abstract

Tuna is one of the most valuable fishery commodities produced in Indonesia. In a great measure of tuna production in Indonesia is exported to several destination countries such as Japan, the European Union and America. The demand for tuna in Japan and the United States from year to year has never decreased. Furthermore, the role of tuna industry from time to time is increasingly important and strategic, especially in sustaining the nation's economy as tuna plays an important role in many people?s health in which Tuna provides high quality protein. In terms of sustainable tuna consumption, valuable creative activities (VCA) is required to be formulated, so all resources (tangible, intangible, human resources) are able to produce prime fishery products that have high quality standard and high value content, competent, safe, traceable, both nationally and internationally. The Prime fishery products are able to be produced if the fishery industry successfully applies several standards both national and international such as ISO, SNI, CODEX, and standards from importers. In terms of applying those standards, it is worth reviewing few of benefits of standard implementation. Associated with the standards applied by the company, there are studies of the benefits of standard implementation in tuna industry in Muara Baru, DKI Jakarta. The purpose of this study was to assess the benefits of standard implementation in tuna companies. The method used was descriptive and it uses several tests including profitability ratio analysis (net profit margin, return on asset, return on equity, profit margin). The results of this study are that both companies had implemented the standard well, standard understanding by both companies still exist different, while from the financial sector the company was able to increase profits
KAJIAN EFEKTIVITAS IMPLEMENTASI PROGRAM DEMONSTRATION FARM DI KECAMATAN BLANAKAN KABUPATEN SUBANG Rahman, Sofyan; Hubeis, Aida Vitayala S; Trilaksani, Wini
MANAJEMEN IKM: Jurnal Manajemen Pengembangan Industri Kecil Menengah Vol. 10 No. 2 (2015): MANAJEMEN IKM
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (554.913 KB) | DOI: 10.29244/mikm.10.2.173-181

Abstract

In recent years, shrimp farming have experienced a variety of problems, both technical and non-technical, causing the shrimp farm do not work properly (idle). In 2012 the Ministry of Maritime Affairs and fisheries formulate revitalization program which focused on the rehabilitation ponds channel through Demonstration Farm (Demfarm) program. The purpose of this study is to analyze the effectiveness of the implementation of the Demfarm program in the Blanakan District in Subang regency. The data has been analyze using qualitative analysis, quantitative analysis, SWOT analysis (Strengths, Weaknesses, Opportunities, and Threats), and AHP (Analytical Hierarchy Process). The result of the study shows that Demfarm implementation in Blanakan District, Subang Regency is quite effective. Blanakan District in Subang Regency is an ideal area for shrimp farm, the application of Demfarm technology can improve the productivity of farms  from 0-17  up to 7-75 tons / ha, the programs also help to establish the partnership system between farmers and the private sector. Shrimp farms have 0.148 IFE and 0.495 EFE, another most important thing for shrimp farming development in the Blanakan District is technology have 0.341. Shrimp farming worth to be developed, the value of Benefit Cost Ratio (B/C ratio) > 1, the Net Present Value (NPV) generated > 0 Break Event Point (BEP) the lowest production 7000 kg and the highest production is 7.500 kg, for BEP the lowest price is Rp60 000 and the highest price is Rp70.000
EVALUASI DAN PENGEMBANGAN SISTEM MANAJEMEN RANTAI PASOK BANDENG SEGAR (CHANOS CHANOS) DI KOTA BEKASI, JAWA BARAT Widria, Yefni; Trilaksani, Wini; Cahyadi, Eko Ruddy
MANAJEMEN IKM: Jurnal Manajemen Pengembangan Industri Kecil Menengah Vol. 11 No. 2 (2016): Manajemen IKM
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (624.118 KB) | DOI: 10.29244/mikm.11.2.129-140

Abstract

Case study is conducted towards 12 SME milkfish processors in Bekasi, at which its raw materials are originated from Karawang Regency through a fresh milkfish supply chain. The potentcy of Karawang Regency as a milkfish producer does not guarantee continuous availability of the raw materials. The objectives of this study were: (1) To evaluate supply chain; and (2) To compile a priority strategy of fresh milkfish supply chain management in Bekasi, West Java. Data collection was conducted by purposive and snowball sampling techniques, starting from SME processors, retailers and collective traders in Bekasi, to milkfish farmers in Ciparage Jaya Village, Tempuran Sub-District, Karawang Regency. The study used descriptive analysis, evaluation by comparing the activities of the members of the supply chain with the existing standards, Strength, Weaknesses, Opportunities and Threats (SWOT) analysis and Analytic Hierarchy Process (AHP). The evaluation result showed there was gap between the activities of each supply chain member with the Indonesian National Standard (SNI) namely (1) implementation of GMP and SSOP by SME processors, (2) means of transport and storage of fresh fish by collective trader and retailer, (3) good aquaculture practices by farmer and (4) supply chain management by all members along the supply chain. Based on the SWOT and AHP matrix analyses, the development strategies of supply chain by actor was (1) the formation of raw material procurement network and marketing network by SME processor (0.326); (2) the quality improvement of human resource by the government (0.249); (3) the formation of organizations by farmer (0.230), and (4) maintaining partnership in business procurement cost by collective trader (0.195).
FORMULATION OF HYDROCOLLOID-AGAR, SUCROSE, AND ACIDULANT ON JAM LEATHER PRODUCT DEVELOPMENT Ramadhan, Wahyu; Trilaksani, Wini
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 20 No. 1 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.934 KB) | DOI: 10.17844/jphpi.v20i1.16495

Abstract

Tallying agar powder as a texturizer in guava single sheet jam instigate the product more convenience to consumed. The aims of this research were to determine the best concentration of sucrose, citric acid and agar powder to form a good quality guava jam slice. The research method are optimization and formulationof sucrose, citric acid and agar-agar on making guava jam single sheets product. Physochemical and sensory tests were performed to reveal the best formulation of guava jam slice and the Bayes method used to determine the optimization of the selected formula. Based on the results of formulation and analysis, itwas obtained that  the guava jam slice with Acidulant concentration (0.02%, 0.04%, 0.06%), sucrose (70%, 80%, 90%, 100%) and agar powder (0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%) had pH 3.63-3.90, sugar content 34.68 g/100 g ? 35.76 g/100 g, color intensity L*, a*, b* with ?E* value was 37,88-53,97, fiber content 1.01%-1.59%, and water activity 0.852-0.893. Rheology properties for texture profile (hardness, cohesiveness, springiness, adhesive force, and gumminess) also showed significant value with agar powder formulation. Based on the Bayes test and hedonic test, it was found that the best formula was for guava jam slices with the addition of 90% sucrose, citric acid 0.04% and agar powder 0.9%. From the best formula, it was found the shelf life prediction model of Arrhenius formula was ln k = 20.222-6660.6(1/T) and the nutrition facts contribute total energy 45 kcal, fat 0%, carbohydrate 9%, protein 2% and dietary fiber 3%.