Nina Tristina
Department of Clinical Pathology

Published : 12 Documents
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Creatine Phosphokinase and Visual Analogue Scale as Indicators for Muscle Injury in Untrained Bodybuilders Shanmugam, Suresh; Farenia, Reni; Tristina, Nina
Althea Medical Journal Vol 2, No 2 (2015)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.344 KB)

Abstract

Background: Skeletal muscle is a vital tissue in the human body to enable breathing, walking and performing several sports activities. However, this muscle is persistently injured throughout every sports session. Some exercises demand a muscle injury occurrence in order to build a stronger muscle through an adaptation process namely bodybuilding exercise. Importantly, every muscle injury should occur within a physiological range which can be identified by several biomarkers as well as pain scale. The aim of this study was toidentify changes on the level of Creatine phosphokinase (CPK) and Visual analogue scale (VAS) between pre and post training sessions and the correlation between these two indicators.Methods: This was an observational analytical cross sectional comparison study which was conducted in October 2012 and the subjects were adult untrained bodybuilders at the Jatinangor fitness center. The data was obtained by measuring serum CPK and marked VAS. The data were analyzed by t-test, Wilcoxon’s test and Spearman’s correlation.Results: Both CPK and VAS increased significantly by 296 U/L and 19.9 mm respectively. There was a strong positive significant correlation between VAS and CPK (p=0.01, r = 0.711).Conclusion: The healthy untrained bodybuilders chosen in this study experienced a mild (<2000 U/L) muscle injury throughout the training sessions with general increased CPK levels and VAS measurement. [AMJ.2015;2(1):147–52]
KADAR N-TERMINAL PRO-BRAIN NATRIURETIC PEPTIDE SEBAGAI PREDIKTOR LUARAN KLINIS SINDROM KORONER AKUT Tandhana, Florencia Idajanti; Noormartany, Noormartany; Aprami, Toni M.; Tristina, Nina
Majalah Kedokteran Bandung Vol 44, No 2 (2012)
Publisher : Faculty of Medicine, Universitas Padjadjaran

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Abstract

Kadar N-terminal pro-brain natriuretic peptide (NT-proBNP) plasma dapat menggambarkan tingkat keparahan iskemia walaupun tidak terjadi nekrosis, iskemia yang transien dapat meningkatkan peregangan dinding jantung yang akan menginduksi sintesis dan pelepasan brain natriuretic peptide (BNP) yang sebanding dengan tingkat keparahan iskemia. Tujuan penelitian untuk mengetahui apakah kadar NT-proBNP pada penderita sindrom koroner akut (SKA) dapat digunakan sebagai parameter prediktor luaran klinis. Penelitian dilakukan sejak bulan Januari hingga Maret 2010. Subjek penelitian penderita SKA yang datang ke Unit Gawat Darurat Rumah Sakit Dr. Hasan Sadikin Bandung dan telah didiagnosis klinis sesuai kriteria World Health Organization. Pada subjek yang memenuhi kriteria inklusi dilakukan pemeriksaan kadar NT-pro BNP dengan metode electrochemiluminescence immunoassay, cardiac troponin T (kuantitatif), dan creatine kinase muscle brain (enzimatik). Analisis data uji normalitas menggunakan one-sample Kolmogorov-Smirnov test, analisis regresi logistik multipel untuk mengetahui parameter prediktor luaran klinis penderita SKA. Dari 83 subjek yang ikut dalam penelitian, didapatkan nilai prediksi kadar NT-proBNP sebesar 1,00 sehingga bukan merupakan prediktor utama luaran klinis, koefisien ? NTproBNP sebesar 0,001 menyatakan bahwa setiap penambahan 1.000 pg/mL variabel NT-proBNP akan menambah lama perawatan 1 hari. Pada subjek SKA dengan luaran (outcome) sembuh nilai prediksi cTnT lebih baik sebagai faktor prediktor dibandingkan dengan konsentrasi NT-proBNP (OR=32,53; 95%IK; 0,58?1.819,26). Simpulan, NT-proBNP bukan merupakan prediktor utama luaran klinis pada SKA. Kadar NT-proBNP lebih dari 826,7 pg/mLterdapat kemungkinan prognosis yang buruk sampai dengan kematian. [MKB. 2012;44(2):106?13].The Role of NT-proBNP as Clinical Outcome Predictor for Acute Coronary SyndromesPlasma levels of N-terminal pro-brain natriuretic peptide (NT-proBNP) levels may reflect the severity of ischemia, although there is no necrosis. A transient ischemia which can increase the heart wall stretch would induces BNP synthesis and release. Synthesis and release of BNP are comparable with the severity of ischemia. The aim of this study was to analyze whether NT-proBNP levels in patients with acute coronary syndrome (ACS) can be used as a predictor for clinical outcome. Studies was held since January to March 2010. Subject were patients with ACS who came to emergency room Dr. Hasan Sadikin Hospital Bandung and were clinically diagnosed according to World Health Organization criteria. Subjects which were suited with the inclusion criteria, stored until assayed. NT-pro BNP concentration was examined by electrochemiluminescence immunoassay method along with creatine kinase muscle brain (enzymatic method) and cardiac troponin T (quantitative method). Statistical analysis was performed using the one-sample Kolmogorov-Smirnov test for verifying normality, normally distributed data were analyzed using parametric analysis and abnormal distributed data was assayed using multiple logistic regression analysis to determine the parameters which can be used as predictor for clinical outcome in patients with ACS. Multiple logistic regression analysis on 83 subjects showed predictive value of NT-proBNP levels with OR=1.00, which mean there was no different likelihood in patients with high and low concentration of NT-proBNP to have longer hospitality duration. NT-proBNP ? coefficient of 0.001 states that every addition of 1,000 pg/mL of NT-proBNP concentration will increase the length of hospitality duration for one day. On convalesce subjects, the most significant predictive value for predicting clinical outcome cTnT was more better than NT-proBNP concentration in patients with ACS (OR=32.53, 95%CI; 0.58?1,819.26). In conclusions, NT-proBNP is not a major predictor of clinical outome in ACS. NT-proBNP levels of >826.7 pg/mL implies a poor prognosis to death. [MKB. 2012;44(2):106?13]. DOI: http://dx.doi.org/10.15395/mkb.v44n2.132 
Correlation between Serum Uric Acid and HbA1c Levels in Patients with Type 2 Diabetes Mellitus Nugraha, Rifan; Tristina, Nina; Miftanurachman, Miftanurachman
Althea Medical Journal Vol 5, No 3 (2018)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.781 KB) | DOI: 10.15850/amj.v5n3.465

Abstract

Background: Indonesia ranks fourth among countries with the highest number of diabetics. Increasing evidence suggests that hyperuricemia is an independent risk factor for impaired fasting glucose (IFG) and type 2 diabetes.  Some observational studies have identified elevated uric acid concentration as a risk factor for diabetes, while others have found an inverse relationship. The present study was conducted to discover the strength and the direction of the correlation. Methods: This study was an analytical cross-sectional study conducted from May to October 2014. The study subjects were 56 medical records of new outpatients diagnosed with T2DM in the Internal Medicine Polyclinic who underwent laboratory examination at the Clinical Pathology Unit of Dr. Hasan Sadikin General Hospital, January-December 2013. Result: The correlation between HbA1c and uric acid levels on the whole subject revealed a weak but significant negative correlation (r=-0.354, p=0.007). There was an insignificant negative correlation in male subjects (r=-0.405, p=0.120); in female subjects (r=-0.319, p=0.05), the correlation was a weak but insignificant negative correlation.Conclusions: There is a weak correlation between HbA1c and uric acid levels in patients with Diabetes Mellitus type 2. 
Correlation between Levels of Serum Amylase, Lipase and Triglyceride in Acute Pancreatitis Patients Govindarajan, Gunalan; Tristina, Nina
Althea Medical Journal Vol 2, No 1 (2015)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.809 KB)

Abstract

Background: Acute pancreatitis is an inflammation of pancreas associated with reversible pancreatic parenchymal injury. Studies in several countries indicate that the levels of amylase and lipase are usually elevated among patients with acute pancreatitis. Furthermore, hyperlipidemia, mainly high levels of triglycerides, may present in acute pancreatitis. The aim of this study was to determine the levels of serum amylase and lipase as well as their correlation with serum triglyceride level in acute pancreatitis patients.Methods: A retrospective study was conducted on medical records of 48 acute pancreatitis patients in Dr. Hasan Sadikin General Hospital Bandung, Indonesia from 2007to 2011. Data collected from the medical records were age, sex, levels of serum amylase, lipase and triglyceride. The distribution of data was determined using Shapiro-Wilk test. The correlation between serum pancreatic enzyme and triglyceride was analyzed using Spearman-rank test.Results: Most patients had increased levels of serum amylase and lipase in this study. However, no correlation between serum amylase and triglyceride (p-value = 0.312) was found. Furthermore, there was no correlation between serum lipase and triglyceride (p-value = 0.241).Conclusions: The levels of serum amylase and lipase increase in most patients with acute pancreatitis with no significant correlation between serum pancreatic enzymes (amylase and lipase) and triglyceride.Keywords: Acute pancreatitis, amylase, lipase, triglyceride DOI: 10.15850/amj.v2n1.420    
Pathogen Profile of Patients with Sepsis in Internal Medicine, Dr. Hasan Sadikin General Hospital, Bandung 2013 Fauzi, Afiq Syazwan; Sumardi, Uun; Tristina, Nina
Althea Medical Journal Vol 3, No 2 (2016)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.74 KB)

Abstract

Background: Sepsis is a continuous disease which begins with systemic inflammatory response syndrome (SIRS), seen in association with a large number of clinical conditions. These include infectious insults that produce SIRS, such as pancreatitis, ischemia, multiple traumas and tissue injury, hemorrhagic shock, immunemediated organ injury, and the exogenous administration of such putative mediators of the inflammatory process as tumor necrosis factor and other cytokines. A frequent complication of SIRS is the development of organ system dysfunction, including such well-defined clinical conditions as acute lung injury, shock, renal failure, and multiple organ dysfunction syndrome (MODS). Hence, this study was conducted to identify the pathogen profile that often causes sepsis.Methods: A retrospective study was performed to 152 medical records of patients diagnosed as sepsis from Internal Medicine Department Dr. Hasan Sadikin General Hospital from January 2013 to December 2013. The variables observed from the medical records were age, sex, comorbidity, main infection, culture sample, type of gram bacteria, resistant bacteria, and antibiotic susceptibility test. After data collection was completed, the data were analyzed using computer. The data were presented in percentage.Results: Sepsis in male was higher than female. Highest comorbid was chronic kidney disease (CKD). The main infection was health care acquired pneumonia (HCAP). Highest pathogen that caused sepsis was Escherichia coli and highest multidrug-resistant organism (MDRO) was extended spectrum beta-lactamase(ESBL) Escherichia coli.Conclusions: The most common pathogen that causes sepsis is Escherichia coli. [AMJ.2016;3(2):200–5]DOI: 10.15850/amj.v3n2.785
Correlation between Serum Uric Acid and HbA1c Levels in Patients with Type 2 Diabetes Mellitus Nugraha, Rifan; Tristina, Nina; Miftanurachman, Miftanurachman
Althea Medical Journal Vol 5, No 3 (2018)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.781 KB) | DOI: 10.15850/amj.v5n3.465

Abstract

Background: Indonesia ranks fourth among countries with the highest number of diabetics. Increasing evidence suggests that hyperuricemia is an independent risk factor for impaired fasting glucose (IFG) and type 2 diabetes.  Some observational studies have identified elevated uric acid concentration as a risk factor for diabetes, while others have found an inverse relationship. The present study was conducted to discover the strength and the direction of the correlation. Methods: This study was an analytical cross-sectional study conducted from May to October 2014. The study subjects were 56 medical records of new outpatients diagnosed with T2DM in the Internal Medicine Polyclinic who underwent laboratory examination at the Clinical Pathology Unit of Dr. Hasan Sadikin General Hospital, January-December 2013. Result: The correlation between HbA1c and uric acid levels on the whole subject revealed a weak but significant negative correlation (r=-0.354, p=0.007). There was an insignificant negative correlation in male subjects (r=-0.405, p=0.120); in female subjects (r=-0.319, p=0.05), the correlation was a weak but insignificant negative correlation.Conclusions: There is a weak correlation between HbA1c and uric acid levels in patients with Diabetes Mellitus type 2. 
Correlation between Levels of Serum Amylase, Lipase and Triglyceride in Acute Pancreatitis Patients Govindarajan, Gunalan; Tristina, Nina
Althea Medical Journal Vol 2, No 1 (2015)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.809 KB)

Abstract

Background: Acute pancreatitis is an inflammation of pancreas associated with reversible pancreatic parenchymal injury. Studies in several countries indicate that the levels of amylase and lipase are usually elevated among patients with acute pancreatitis. Furthermore, hyperlipidemia, mainly high levels of triglycerides, may present in acute pancreatitis. The aim of this study was to determine the levels of serum amylase and lipase as well as their correlation with serum triglyceride level in acute pancreatitis patients.Methods: A retrospective study was conducted on medical records of 48 acute pancreatitis patients in Dr. Hasan Sadikin General Hospital Bandung, Indonesia from 2007to 2011. Data collected from the medical records were age, sex, levels of serum amylase, lipase and triglyceride. The distribution of data was determined using Shapiro-Wilk test. The correlation between serum pancreatic enzyme and triglyceride was analyzed using Spearman-rank test.Results: Most patients had increased levels of serum amylase and lipase in this study. However, no correlation between serum amylase and triglyceride (p-value = 0.312) was found. Furthermore, there was no correlation between serum lipase and triglyceride (p-value = 0.241).Conclusions: The levels of serum amylase and lipase increase in most patients with acute pancreatitis with no significant correlation between serum pancreatic enzymes (amylase and lipase) and triglyceride.Keywords: Acute pancreatitis, amylase, lipase, triglyceride DOI: 10.15850/amj.v2n1.420    
Pathogen Profile of Patients with Sepsis in Internal Medicine, Dr. Hasan Sadikin General Hospital, Bandung 2013 Fauzi, Afiq Syazwan; Sumardi, Uun; Tristina, Nina
Althea Medical Journal Vol 3, No 2 (2016)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.74 KB)

Abstract

Background: Sepsis is a continuous disease which begins with systemic inflammatory response syndrome (SIRS), seen in association with a large number of clinical conditions. These include infectious insults that produce SIRS, such as pancreatitis, ischemia, multiple traumas and tissue injury, hemorrhagic shock, immunemediated organ injury, and the exogenous administration of such putative mediators of the inflammatory process as tumor necrosis factor and other cytokines. A frequent complication of SIRS is the development of organ system dysfunction, including such well-defined clinical conditions as acute lung injury, shock, renal failure, and multiple organ dysfunction syndrome (MODS). Hence, this study was conducted to identify the pathogen profile that often causes sepsis.Methods: A retrospective study was performed to 152 medical records of patients diagnosed as sepsis from Internal Medicine Department Dr. Hasan Sadikin General Hospital from January 2013 to December 2013. The variables observed from the medical records were age, sex, comorbidity, main infection, culture sample, type of gram bacteria, resistant bacteria, and antibiotic susceptibility test. After data collection was completed, the data were analyzed using computer. The data were presented in percentage.Results: Sepsis in male was higher than female. Highest comorbid was chronic kidney disease (CKD). The main infection was health care acquired pneumonia (HCAP). Highest pathogen that caused sepsis was Escherichia coli and highest multidrug-resistant organism (MDRO) was extended spectrum beta-lactamase(ESBL) Escherichia coli.Conclusions: The most common pathogen that causes sepsis is Escherichia coli. [AMJ.2016;3(2):200–5]DOI: 10.15850/amj.v3n2.785
Creatine Phosphokinase and Visual Analogue Scale as Indicators for Muscle Injury in Untrained Bodybuilders Shanmugam, Suresh; Farenia, Reni; Tristina, Nina
Althea Medical Journal Vol 2, No 2 (2015)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.344 KB)

Abstract

Background: Skeletal muscle is a vital tissue in the human body to enable breathing, walking and performing several sports activities. However, this muscle is persistently injured throughout every sports session. Some exercises demand a muscle injury occurrence in order to build a stronger muscle through an adaptation process namely bodybuilding exercise. Importantly, every muscle injury should occur within a physiological range which can be identified by several biomarkers as well as pain scale. The aim of this study was toidentify changes on the level of Creatine phosphokinase (CPK) and Visual analogue scale (VAS) between pre and post training sessions and the correlation between these two indicators.Methods: This was an observational analytical cross sectional comparison study which was conducted in October 2012 and the subjects were adult untrained bodybuilders at the Jatinangor fitness center. The data was obtained by measuring serum CPK and marked VAS. The data were analyzed by t-test, Wilcoxon’s test and Spearman’s correlation.Results: Both CPK and VAS increased significantly by 296 U/L and 19.9 mm respectively. There was a strong positive significant correlation between VAS and CPK (p=0.01, r = 0.711).Conclusion: The healthy untrained bodybuilders chosen in this study experienced a mild (<2000 U/L) muscle injury throughout the training sessions with general increased CPK levels and VAS measurement. [AMJ.2015;2(1):147–52]
HYBRIDIZATION-BASED NUCLEIC ACID AMPLIFICATION TEST TERHADAP CARTRIDGE-BASED NUCLEIC ACID AMPLIFICATION TEST TERKAIT MULTIDRUG-RESISTANT TUBERCULOSIS (Hybridization-Based Nucleic Acid Amplification Test towards Catridge-Based Nucleic Acid Amplification Test in Multidrug-Resistant Tuberculosis) Sulianto, Ivana Agnes; Parwati, Ida; Tristina, Nina; I, Agnes Rengga
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1274

Abstract

Indonesia has high burden of multidrug-resistant tuberculosis (MDR-TB). Cartridge-based nucleic acid amplification test (CB-NAAT),which is recommended as a diagnostic method of MDR-TB by World Health Organization, is faster in achieving the result. This methoddetermines MDR-TB only from the rifampisin resistance, by detecting mutations that occur on the 81 bp hot-spot region of the rpoBgene. The isoniazid resistance is not included in the determination of MDR-TB by this method. Hybridization-based NAAT (HB-NAAT)detects MDR-TB not only from the rifampisin resistance (codon 526 and 531 rpoB gene), but also from the isoniazid resistance (codon315 katG gene). The aim of this study was to know the validity of the HB-NAAT in detecting MDR-TB using sputum with CB-NAATas the gold standard in a diagnostic study. All of 51 sputums were collected during June 2013 from patients suspected pulmonaryMDR-TB at Dr. Hasan Sadikin General Hospital. The result of CB-NAAT were 16 MDR-TB, 12 TB non MDR, and 23 non TB. HB-NAATexamination results were 3 MDR-TB, 25 TB non MDR (3 RMR, 6 IMR, 16 susceptible) and 23 non TB. The sensitivity of HB-NAAT was18.75% and specificity 100%. Low sensitivity values may due to the high mutation variations in the samples. So it could not be detectedonly by codons 526 and 531 for rifampisin resistance. For the detection of isoniazid resistance, HB-NAAT have optimal primer at lowconcentrations and it also need more than katG genes to detect isoniazid resistance. Based on this study, it can be conclued, that HBNAAThas low sensitivity but high specificity in the detecting MDR-TB.