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IDENTIFIKASI PENYAKIT BAKTERIAL PADA BENIH SIDAT (ANGUILLA MARMORATA) DI BALAI BUDIDAYA AIR TAWAR TATELU Kusen, Kevin Octavian; Tumbol, Reiny A; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.1.2015.6935

Abstract

This study aimed to identify the bacterial disease in cultured eel elvers (Anguilla marmorata). The samples used for this study were taken from the Tatelu Freshwater Aquaculture Center. The samples taken were elvers (juvenile life stage of A. marmorata)  which showed clinical symptoms of bacterial infection such as bleeding in the chest, abdomen, and the base of the fin, passive movement, weakness, and loss of balance of the body, loss of appetite, some wounds on back fin, chest, and tail, and the body was not slippery/shiny. Three elvers with the size of 4-6 cm, and weigh about 0.2 g were taken as samples. The organ chosen to be targeted for isolation was head kidney. Each sample was isolated twice as replicates and streaked on Triptic Soy Agar (TSA) medium. First replicates were identified in the Tatelu Health Lab of Freshwater Aquaculture, and the second replicates were identified in North Sulawesi Provincial Health Lab. Bacterial identification was done through a series of observations and morphology of bacterial colonies through gram stain, followed by biochemical tests through oxidase test, catalase test, Triple Sugar Iron Agar (TSIA) test, H2S production test, motility test, O/F test, and Simmons Citrate Agar (SCA) test. Water quality parameters that were measured included temperature, disolve oxygen, pH, and ammonia. Based on identification of bacteria in cultured elvers in Tatelu Freshwater Aquaculture Center, the elver samples had been infected with bacterial  Aeromonas hydrophila. Keywords: elver, identification, Aeromonas hydrophila, Tatelu Freshwater Aquaculture Center
IDENTIFIKASI KOI HERVES VIRUS PADA IKAN MAS CYPRINUS CARPIO DI SULAWESI UTARA TAHUN 2017 DENGAN MENGGUNAKAN TEKNIK PCR DAN QPCR Sultan, Makkulau; Wullur, Stenly; Tumbol, Reiny A
JURNAL PESISIR DAN LAUT TROPIS Vol 6, No 2 (2018): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.6.2.2018.21521

Abstract

This research aimed to detect the distribution of KHV disease in cultured common carps using conventional PCR and Real Time Quantitative PCR methods in North Sulawesi. The samples were taken from 6 aqua culture centres in North Sulawesi. The results of KHV detection by PCR method showed negative KHV infection because visualization does not form a specific band with the KHV gene that is at 409 bp. Detection of KHV of Ct (Quantification cycle) was greater than the LOD with a confidence level of 95% where Ct LOD is 8.71 for the smallest standard of 1.0x102 copies. Ct sample that was read based on qPCR amplification result which was 14,69-18,80 and the value of Ct NTC (Non Template Control) used as a negative control was 17.52.Penelitian ini bertujuan untuk mengidentifikasi keberadaan penyakit Koi Herves Virus pada ikan mas dengan menggunakan metode PCR dan qPCR di Sulawesi Utara. Sampel uji diambil dari 6 sentral budidaya di Provinsi Sulawesi Utara. Hasil penelitian menunjukkan bahwa dengan metode PCR diperoleh hasil deteksi yang negatif, karena visualisasi tidak terbentuk band spesifik dengan KHV, yaitu di 409 bp. Deteksi KHV dengan metode qPCR didapat hasil infeksi KHV yang negatif dilihat dari nilai rata-rata Ct (Quantification cycle) lebih besar dari LOD dengan tingkat kepercayaan (confident level) 95%. Nilai Ct LOD adalah 8,71 untuk standar terkecil 1,0x102 copies, sedangkan Ct sampel hasil amplifikasi qPCR adalah 14,69-18,80 dan nilai Ct NTC (Non Template Control) yang digunakan sebagai kontrol negatif adalah 17,52.
DIAGNOSA PENYAKIT BAKTERIAL PADA IKAN NILA (OREOCRHOMIS NILOTICUS) YANG DI BUDI DAYA PADA JARING TANCAP DI DANAU TONDANO Ashari, Chairanitansyah; Tumbol, Reiny A; Kolopita, Magdalena E.F
e-Journal BUDIDAYA PERAIRAN Vol 2, No 3 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.2.3.2014.5700

Abstract

This research aimed to diagnose bacterial diseases on nile tilapia cultured in stick net cage in Lake Tondano. Fish samples were taken from three locations of net cages in Lake Tondano namely Paleloan, Eris and Remboken villages. Three samples of fish were taken from each location/village. The samples taken showed clinical symptoms of bacterial infection such as hemorrhagic on the abdomen, the base of the fin, anal and chest, irregular swimming fish, hemorrhagic on the gills, fish refuse food, remain silent on the outskirts of net cage. Bacteria were isolated by using a sterile loop from head kidney (anterior kidney), which is located on the front, below the spine near the head. Bacterial cultures were performed on TSA media by making a zigzag scratches. The petri dish containing bacterial isolates were subsequently incubated in an incubator at a temperature of 35oC for 24 hours. The water quality parameter data measured consisted of temperature, Dissolved Oxygen, pH, ammonia, brightness. The results of the study showed that farmed tilapia in Lake Tondano has been infected with Aeromonas hydrophila that causes MAS (Motile Aeromonad Septicemia). The water quality, both chemical and physics around the net cages in the Lake Tondano was still in good condition and suitable for fish cultivation.   Keywords: Oreocrhomis niloticus, Bacterial disease, septicemia aeromonad motile, fixed net cage, Lake Tondano
KELAYAKAN LOKASI BUDIDAYA IKAN DI DANAU TONDANO DITINJAU DARI PARAMETER FISIKA KIMIA AIR Kamsuri, Agus I; Pangemanan, Penky N.L; Tumbol, Reiny A
e-Journal BUDIDAYA PERAIRAN Vol 1, No 3 (2013)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.1.3.2013.2732

Abstract

The purpose of this study was to determine the current condition of the water quality of Lake Tondano in terms of physical and chemical parameters in a fish farming locations on Lake Tondano waters. Determination of sampling points at each station is placed vertically at three predetermined points from the guard house toward the front of the net, the distance between one point to the next point was ± 10 m; whereas for the analysis of water quality parameters was done in Clinical Pathology of Fish Diseases Laboratory, Faculty of Fisheries and Marine Science, Sam Ratulangi Manado and Manado Industrial Research and Standards Laboratory. Determination points were done by purposive sampling which refers to the physiographic location wherever possible in order to represent or describe these waters. The research was carried out for 6 weeks and was done in 2 stages, morning and afternoon. For direct measurement (in situ) was performed once a week which included parameters DO, pH, temperature, and brightness, while the laboratory tests were conducted for 2 weeks which included parameters Nitrite, Nitrate, Iron, Chlorine, Manganese, Chloride, Sulfate, Aluminum, ammonia and Phosphate. The results show that the locations of the four parameters of chlorine (CL2) with range (0.1 to 0.28) and the parameters of Ammonia with the range (0.0125 to 0.15) over the limit indicated on water quality standards. The parameters of temperature, DO, pH, brightness, nitrate, nitrite, sulfate, manganese, chloride, aluminum, iron and phosphate were still in the water quality standard PP No.82 of 2001.
EVALUATION OF BAKER’S YEAST (SACCHAROMYCES CEREVICIAE) IN ENHANCING NON SPECIFIC IMMUNE RESPONSE AND GROWTH OF NILE TILAPIA Manurung, Usy N; Manoppo, Henky; Tumbol, Reiny A
e-Journal BUDIDAYA PERAIRAN Vol 1, No 1 (2013): Vol.1 No. 1 Januari 2013
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.1.1.2013.720

Abstract

This research was conducted to evaluate the efficacy of baker?s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker?s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker?s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker?s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker?s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker?s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker?s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus
DETERMINASI MOLEKULER KOI HERPES VIRUS (KHV) YANG DIISOLASI DARI IKAN KOI (CYPRINUS CARPIO KOI) Saselah, Jetti Treslah; Tumbol, Reiny A; Manoppo, Henky
JURNAL PERIKANAN DAN KELAUTAN TROPIS Vol 8, No 2 (2012)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jpkt.8.2.2012.408

Abstract

Penelitian ini bertujuan untuk mendeterminasi keberadaan Koi Herpes Virus (KHV) pada ikan koi (Cyprinus carpio koi). Sampel ikan diambil dari Kabupaten Kepulauan Sangihe. Penelitian di lakukan di Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sam Ratulangi dengan menggunakan metode Polymerase Chain Reaction (PCR). Hasil pemeriksaan PCR terhadap sampel ikan koi mengindikasikan bahwa sampel ikan koi telah terinfeksi oleh virus KHV, yang ditandai dengan munculnya pita DNA pada hasil visualisasi elektroforesis agarosa. Hasil pemeriksaan pada morfologi ternyata sampel ikan yang digunakan menunjukkan gejala-gejala klinis terserang KHV seperti mata pucat, insang berwarna pucat serta produksi lendir yang berlebihan. Beberapa sampel lainnya walaupun secara morfologi belum menunjukan gejala-gejala klinis tetapi melalui pemeriksaan PCR telah terindikasikan terinfeksi KHV. Kata kunci: KHV, PCR, ikan koi   The purpose of this study was to determine the presence of Koi Herpes Virus (KHV) in Koi (Cyprinus carpio koi). Fish samples were taken from the Sangihe Archipelago Regency. Laboratory work conducted at the Laboratory of Biology Faculty of Mathematics and Natural Sciences University of Sam Ratulangi using Polymerase Chain Reaction (PCR) method. The samples indicated that the koi fish samples were infected with KHV. It is shown by the appearance of DNA bands on agarose electrophore­sis visualization. The morphological examination of the body indicated clinical symptoms of KHV infection, such as pale eyes, pale gills and excessive mucus production. Several other samples, although without clinical symptoms, have shown to be infected as indicated by PCR test. Keywords: KHV, PCR, Koi.
THE ROLE OF BAKASANG AS IMMUNOSTIMULANT ON NON-SPECIFIC IMMUNE RESPONSE IN NILE TILAPIA (OREOCHROMIS NILOTICUS) Pangaribuan, Rosa D; Tumbol, Reiny A; Manoppo, Hengky; Sampekalo, Julius
AQUATIC SCIENCE & MANAGEMENT Vol 1, No 2 (2013): Oktober
Publisher : Graduate Program of Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.1.2.2013.7280

Abstract

Bakasang produced from fermented fish?s offals contains some type of Lactic Acid Bacteria (LAB) and have potential as imunostimulant. LAB that can live and grow in the digestive tract of fish serve to suppress the growth of pathogenic bacteria, and produce metabolites that can stimulate the activity of the immune system. The purpose of this study was to examine the effect of bakasang as imunostimulant and to determine the optimal dose of bakasang for increasing non-specific immune response and growth in tilapia (Oreochronomis niloticus). This research was conducted using completely randomized design with four treatments and three replicates: B0 (0 ml/kg feed), B1 (50 ml/kg feed), B2 (100 ml/kg feed), and B3 (150 ml/kg feed). The treatment feed was given for 4 weeks at a dose of 3% /body weight/day with a frequency of twice a day (08:00 and 17:00). The data taken were immune parameters (total leukocytes and phagocytic activity) and growth. To evaluate the effect of bakasang, the observed parameters were subjected to analysis of variance performed to evaluate differences between the treatments. The results show that after 4 weeks of feeding, the total leukocyte of tilapia treated with bakasang B2 (100 ml/kg feed) on week three was significantly different compared to the total leukocytes in the other treatments with total leukocytes of 68% more than the control. Phagocytic activity in treated fish with 100 and 150 ml/kg (Treatment B2 and B3) were significantly different (p<0.05) from the other treatments. Nevertheless, the phagocytic activity in treatment B2 (100 ml/kg) was higher than B3 (150 ml/kg). Bakasang has an influence on growth during 4 weeks treatment in B1 and B2 which were significantly different to other treatments, but the difference between B1 and B2 treatment was not significantly different. The weight gain of tilapia in treatment B1 was 17.06 ± 3.17 g or 34.75% more than the control treatment, while the B2 body weight reached 17.72 ± 2.63 g or 39.96% greater than the control. In conclusion, the inclusion of bakasang in fish feed by using oral technique with a dose of 100 ml/kg could increase the nonspecific immune response and growth of tilapia. Bakasang yang dihasilkan dari fermentasi jeroan ikan mengandung beberapa jenis Bakteri Asam Laktat (BAL) dan mempunyai potensi sebagai immunostimulan. BAL, yang dapat hidup dan tumbuh di dalam saluran pencernaan, berfungsi menekan pertumbuhan bakteri patogen dan menghasilkan produk metabolit yang dapat merangsang aktivitas sistem kekebalan tubuh. Tujuan dari penelitian ini adalah untuk menguji pengaruh bakasang sebagai imunostimulan serta menentukan  dosis yang optimal  dalam meningkatkan respon imun non spesifik dan pertumbuhan pada ikan nila (Oreochronomis niloticus). Penelitian dilaksanakan menggunakan  Rancangan Acak Lengkap dengan empat perlakuan B0 (0 ml/kg pakan), B1 (50 ml/kg pakan), B2 (100 ml/kg pakan), dan B3 (150 ml/kg pakan); masing-masing dengan tiga ulangan.  Pakan perlakuan diberikan selama 4 minggu dengan dosis sebanyak 3%/bb/hari dengan frekwensi pemberian 2x sehari pagi (08.00), dan sore (17.00). Data yang diamati terdiri dari parameter imun (total leukosit dan aktivitas fagositik) dan pertumbuhan. Untuk mengevaluasi pengaruh bakasang terhadap parameter yang diamati dilakukan analisis ragam, sedangkan untuk mengevaluasi perbedaan pengaruh antar perlakuan dilakukan Uji Duncan. Setelah diberikan selama  4 minggu, total leukosit ikan nila yang diberi perlakuan bakasang  B2 (100 ml/kg pakan) minggu ke-3 berbeda sangat nyata dibandingkan dengan total leukosit pada perlakuan lainnya dengan total leukosit mencapai 68% lebih banyak dari kontrol. Aktivitas fagositosis pada ikan yang diberi perlakuan 100 ml/kg dan 150 ml/kg (Perlakuan B2 dan B3 ) berbeda nyata (p< 0.05) dengan perlakuan lainnya. Meskipun demikian aktivitas fagositosis pada perlakuan B2 (100 ml/kg) lebih tinggi dibandingkan pada perlakuan B3 (150 ml/kg). Pengaruh bakasang  terhadap pertumbuhan selama minggu ke 4 perlakuan B1 dan B2 berbeda nyata dibandingkan dengan perlakuan lainnya, namun antar perlakuan B1 dan B2 tidak berbeda nyata. Perolehan berat ikan nila pada perlakuan  B1 sebesar 17,06 ± 3,17 g atau 34,75% lebih berat dari kontrol, sedangkan pada perlakuan B2 berat tubuh mencapai  17,72 ± 2,63 g atau 39,96% lebih besar dari kontrol. Sebagai kesimpulan, pemberian bakasang secara oral pada pakan ikan dapat menjadi imunostimulan dan memberikan pengaruh terhadap pertumbuhan ikan dengan dosis 100 ml/ kg pakan.
THE USE OF BAKER’S YEAST (SACCHAROMYCES CEREVICIAE) AS IMMUNOSTIMULANT TO ENHANCE RESISTANCE OF NILE TILAPIA (OREOCHROMIS NILOTICUS) TO AEROMONAS HYDROPHILA Manurung, Usy N; Manoppo, Henky; Tumbol, Reiny A
AQUATIC SCIENCE & MANAGEMENT Edisi Khusus 2 (2014): Oktober
Publisher : Graduate Program of Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.0.0.2014.7300

Abstract

The objective of this research was to evaluate the efficacy of Baker?s Yeast S. cereviciae in enhancing the resistance of nile tilapia (Oreochromis niloticus) to A. hydrophila. As many as 250 fish with an average weight of 28.78±2.44 g were obtained from Fish Culture Development and Training Center of Tateli. After acclimatization, the fish were fed pellet supplemented with Baker?s Yeast as treatments at five different doses, A=0 gr/kg feed, B=5 gr/kg feed, C=10 gr/kg feed, D=15 gr/kg feed, and E=20 gr/kg feed each of which was with three replications.  They were fed for four weeks at 5%/BW/day, twice a day at 08.00 and 16.00, respectively. After feeding period, the fish were challenged intraperitoneally with A. hydrophila.  Before injection, a pathogenicity test of bacteria A. hydrophila was conducted for LD50. Challenged test was carried out by injecting fish with 0.2 ml of bacterial suspension containing 5 x 106 cfu/ml. The fish resistance was observed for 14 days. Dead fish were taken out and bacterial isolation was performed to confirm the cause of the dead.  Results showed that supplementation of Baker?s Yeast into fish pellet had significant effect  on the fish resistance (p=0.00).  The highest resistance (66.6%) was recorded in fish fed with pellet supplement of 5 g Baker?s Yeast per kg of pellet while control fish was only 50%.  As conclusion, supplementation of Baker,s Yeast into fish pellet could enhance resistance of fish to the pathogen. Penelitian ini bertujuan untuk menguji efikasi ragi roti S. cereviciae dalam meningkatkan resistensi ikan nila (Oreochromis niloticus)terhadap bakteri patogen A. Hydrophila. Sebanyak 250 ekor ikan nila dengan berat awal rata-rata 28,78±2,44 g yang diambil dari Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. Setelah aklimatisasi, ikan diberi ragi roti sebagai perlakuan dengan lima dosis berbeda dan masing-masing perlakuan memiliki tiga ulangan.Perlakuan ragi roti yang digunakan adalah A=0 gr/kg pakan, B=5 gr/kg pakan, C=10 gr/kg pakan, D=15 gr/kg pakan, E=20 gr/kg pakan. Lama pemberian pakan perlakuan empat minggu dengan dosis 5%/bb/hari dan diberikan 2 kali sehari yaitu Pukul 08.00 dan Pukul 16.00. Setelah diberikan ragi roti selama empat minggu ikan diuji tantang dengan bakteri A. Hidrophyla secara Intraperopeneal (ip). Sebelum penyuntikan, dilakukan uji patogenitas bakteri yang memberikan tingkat kematian 50% (LD50). Uji tantang dilakukan dengan cara menyuntikkan 0,2 ml suspensi bakteri pada kepadatan 5 x 106 cfu/ml (sesuai hasil uji patogenitas) pada rongga tubuh ikan. Pengamatan resistensi ikan akan dilakukan setiap hari selama 14 hari. Ikan mati dikeluarkan dan dilakukan isolasi bakteri untuk mengkonfirmasi penyebab kematian ikan. Hasil penelitian mendapatkan bahwa pemberian ragi roti berpengaruh sangat nyata (P=0,00) pada resistensi ikan terhadap bakteri A. Hydrophila. Resistensi tertinggi dicapai pada ikan yang diberi perlakuan B (5 g/kg pakan) dengan tingkat resistensi mencapai 66,6%. Sedangkan ikan yang tidak diberi perlakuan ragi roti (control) memiliki resistensi 50 %. Sebagai kesimpulan penambahan ragi roti dalam pakan dapat meningkakan resistensi ikan terhadap infeksi pathogen.
THE USE OF β-GLUCAN EXTRACTED FROM BAKER’S YEAST (SACCHAROMYCES CEREVISIAE) TO INCREASE NON-SPECIFIC IMMUNE SYSTEM AND RESISTENCE OF TILAPIA (OREOCHROMIS NILOTICUS) TO AEROMONAS HYDROPHILA Jamal, Ida N; Tumbol, Reiny A; Mangindaan, Remy E.P
AQUATIC SCIENCE & MANAGEMENT Edisi Khusus 1 (2013): Mei
Publisher : Graduate Program of Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.0.0.2013.2288

Abstract

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the ?-glucan. As immunostimulants, ?-glucans can also increase resistance in farmed tilapia. Studies on the use of ?-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of ?-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of ?-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of  10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian ?-glukan. Sebagai imunostimulan, ?-glukan juga dapat  meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan ?-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis ?-glukan  yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah ?-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©