Zaituni Udin
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Use of pregnant mare’s sera gonadotropin (PMSG) in media in vitro maturation of cow oocytes Udin, Zaituni; ., Jaswandi; Afriani, Tinda; E, Leonardo
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 1 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.149 KB) | DOI: 10.14334/jitv.v12i1.564

Abstract

It is known that hormone addition in media helps in vitro maturation of oocyte. This research was aimmed to determine the effect of PMSG in media to maturation rate and nucleous developvement of cow oocyte. Ovaries were obtainned from local slaughterhouse. The media used for in vitro maturation of oocyte was TCM- 199 and the treatment was 3 levels of PMSG: 0, 10 and 20 mg/ml. Result of this research showed that the dose of PMSG in maturation media was significantly affected (P<0.05)  nucleolus development of oocytes and maturation rate. The average of germinal vesicle (GV) stage in 3 levels of PMSG 0, 10 and 20 mg/ml were 38.33; 12.64 and 9.64%, respectivelly. There was no germinal vesicle breakdown (GVBD) found in 3 levels of PMSG addition. The nucleous development of metaphase–I (M-I) were 7.64; 20.2 and 22.00%, but the average of maturation rate (M-II)  was 16.32; 48.10 and 35.34% for 3 levels of PMSG: 0, 10 and 20 mg/ml, respectivelly. It is concluded that 10 mg/ml PMSG in media of in vitro maturation resuls in the highest maturation rate of cow oocyte. Key Words: Oocyte, Maturation, In vitro, Hormone, PMSG
WAKTU DAN KEMERAHAN VULVA SAAT INSEMINASI BUATAN MERUPAKAN FAKTOR PENENTU ANGKA KEBUNTINGAN SAPI DI SUMATERA BARAT (TIME AND REDDISH SIGN OF VULVA DURINGARTIFICIAL INSEMINATION AS A DETERMINANT FACTORS ON CONCEPTION RATE OF COW IN WEST SUMATERA) Udin, Zaituni; Rahim, Ferdinal; Hendri, Hendri; Yellita, Yulia
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

An experiment was conducted to evaluate determinant factors on conception rate of local and crossbreedcow at West Sumatera. There were 162 cows of four AI center at parity-0 to 4TH parity (P-4), inseminatedusing frozen semen of four different bulls (simental,brahman, limousine and Bali cattle). All fed similarityat four AI centers and location to observation of sign of estrus in high land and low land in West Sumatera.All data were analyzed using analyzed of variance in Randomized Block Design (RBD) in factorial as afactor A was time of inseminated; parity;sign of vulva,breed and two location as a replication. Resultsofthis study showed that there were no interaction between time of insemination, breed, parity and thereddish sign of vulva (P>0.05) on conception rate. On the contrary,there were significantly different(P>0.05) on conception rate by time of insemination. The highest conception rate wasfound at the end ofestrus 13?18 h (68.91%),local cow (71.82%), P-3 (67.19%) and reddish sign of vulva (74.25%). It wasconcluded that the effects,both inseminationtime and reddish sign of vulva might be the important factorto get the highest conception rate of the cows.
Use of pregnant mare’s sera gonadotropin (PMSG) in media in vitro maturation of cow oocytes Udin, Zaituni; ., Jaswandi; Afriani, Tinda; E, Leonardo
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.149 KB) | DOI: 10.14334/jitv.v12i1.564

Abstract

It is known that hormone addition in media helps in vitro maturation of oocyte. This research was aimmed to determine the effect of PMSG in media to maturation rate and nucleous developvement of cow oocyte. Ovaries were obtainned from local slaughterhouse. The media used for in vitro maturation of oocyte was TCM- 199 and the treatment was 3 levels of PMSG: 0, 10 and 20 mg/ml. Result of this research showed that the dose of PMSG in maturation media was significantly affected (P<0.05)  nucleolus development of oocytes and maturation rate. The average of germinal vesicle (GV) stage in 3 levels of PMSG 0, 10 and 20 mg/ml were 38.33; 12.64 and 9.64%, respectivelly. There was no germinal vesicle breakdown (GVBD) found in 3 levels of PMSG addition. The nucleous development of metaphase–I (M-I) were 7.64; 20.2 and 22.00%, but the average of maturation rate (M-II)  was 16.32; 48.10 and 35.34% for 3 levels of PMSG: 0, 10 and 20 mg/ml, respectivelly. It is concluded that 10 mg/ml PMSG in media of in vitro maturation resuls in the highest maturation rate of cow oocyte. Key Words: Oocyte, Maturation, In vitro, Hormone, PMSG
EFFECT OF EXTENDER AND COOLING RATE ON THE QUALITY OF FROZEN THAWED SEMEN OF BALI BULL (BOS SONDAICUS) Farhana, Anna; Udin, Zaituni; Jaswandi, Jaswandi; Aji, Riyan Nugroho
Buletin Peternakan Vol 42, No 4 (2018): BULETIN PETERNAKAN VOL. 42 (4) NOVEMBER 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.285 KB) | DOI: 10.21059/buletinpeternak.v42i4.35173

Abstract

The purpose of this study was to observe the effect of extender and cooling rate on the quality of frozen thawed semen of Bali bull (Bos sondaicus). The experiment was conducted at Laboratory of Animal Biotechnology, Faculty of Animal Husbandry, Andalas University. A completely randomized design of factorial 2 x 3, 2 different extenders and 3 cooling rates with 3 bulls as the replicate. Data were analyzed using analysis of variance (ANOVA). The first factors was extender such as tris citrate egg yolk (TCEY) and tris citrate soy milk (TCSM). The second factors was the cooling rate such as: 15ºC/min, 10ºC/min and 5ºC/min. Variables were: motility, viability, abnormality, and membrane integrity. The results of treatment of TCEY extender on the average of motility 42.67±5.17%, viability of 53.78±3.79%, abnormality of 18.89±1.07%, and membrane integrity of 35.44±3.01%. The effect of cooling rate of 10°C /min has the highest semen motility of 44.35±5.28%, viability of 57.17±1.18%, abnormality of 17.84 ± 0.23 % and membrane integrity of 36.83±2.12%. The interaction between extender and cooling rate was founded significant different (P<0.05). Otherwise of extender and cooling rate on post-thawed of motility, viability and membrane integrity, had no difference (P>0.05) on semen abnormality. It can be concluded that tris-citrate egg yolk (TCEY) extender with cooling rate of 10ºC / min were the best semen motility, viability, and membrane integrity of spermatozoa.
QUALITY DIFFERENCES OF BOER LIQUID SEMEN DURING STORAGE WITH ADDITION SWEETORANGEESSENTIAL OIL IN TRIS YOLK AND GENTAMICIN EXTENDER Sitepu, Sukma Aditya; Udin, Zaituni; Jaswandi, Jaswandi; Hendri, Hendri
Journal of Community Research and Service Vol 1, No 2 (2017)
Publisher : Center of Community Service of State University of Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24111/jcrs.v1i2.9341

Abstract

AbstractThe purpose of this study was to know the quality of Boer liquid semen during storage by adding sweet orange essential oil and gentamicin into the tris yolkextender.The semenlongevity test was carried out by storing semen in a closed tube at room temperature and refrigerator, and evaluated motility and Viability every 3 hours at room temperature and 12 hours in refrigerator. The results showed that the characteristics of the liquid semen, with the addition of 1% of essential oils showed percentage of motility and Viability significantly higher than 0.5% and without the addition of essential oil, either stored at room temperature or in the refrigerator. This may be related to the content of essential oils of sweet orange peel containing flavonoids and antibacterials that are capable of maintaining liquid semen quality of Boer Goat.In addition, gentamicin contains antibacterials capable of suppressing the growth of bacteria which can damage and durabilitythe spermatozoa.Keywords: Boer Goat,Gentamicin, Liquid semen, Sweet orange
Suplementasi Mineral Lokal untuk Perbaikan Nutrisi dan Reproduksi Sapi Peranakan Simmental Dara pada Peternakan Rakyat di Jorong Sibaladuang, Kabupaten Limapuluh Kota Khalil, .; Andri, .; Udin, Zaituni
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 5, No 3 (2019): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.5.3.202-209

Abstract

This community service was aimed to evaluate beneficial effect and constrains of mineral supplementation onfeed of Simmental heifers that be raised by smallholders. Mineral were formulated by using locally available materials (rock flour, limestone, fresh water oyster shell meal) and prepared in loose and lick-block forms. The local mineral formulas were fed to 24 Simmental heifers raised by 17 smallholders for 14 weeks in four treatments: P0 (no supplementation, control), P1 (supplemented with commercial mineral premix), P2 (supplemented with local mineral feed in loose meal), and P3 (supplemented with local mineral feed in form). Each treatment consisted of 6 heifers as replication. Parameters measured included: blood minerals (Ca, P, Mg) and blood hematology (hemoglobin (Hb), mean corpuscular Hb concentration (MCHC), total red blood cell (RBC), white blood cell (WBC), hematocrit concentration (HCT)), total protein, and estrus onset. Results showed that heifers supplemented with local minerals (P2 and P3) were detected earlier estrus onset than the control (P0) and that supplemented with commercial mineral premix (P1). Local mineral supplementation gave also positive effect on blood mineral, protein and hematological profiles, even though there were no statistically difference. It was concluded that mineral supplementation gave positive effect of reproduction performances and nutritional status of Simmental heifers. Local mineral offered in lick-block had better effect than that in loose form.