Niken Ulupi
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Published : 15 Documents
Articles

Found 15 Documents
Search

IDENTIFIKASI KERAGAMAN GEN KALPASTATIN (CAST) PADA AYAM LOKAL INDONESIA (IDENTIFICATION OF POLYMORPHISM CALPASTATINE GENE IN LOCAL CHICKEN) Harahap, Ahmad Saleh; Sumantri, Cece; Ulupi, Niken; Darwati, Sri; Sartika, Tike
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.2.192

Abstract

Calpastatin (CAST) gene is one of the genes that play a role in the process of tenderization of meat. CAST gene serves as an inhibitor of meat tenderness. The purpose of this study was to identify Single Nucleotide Polymorphisms (SNP) in the CAST gene of kampong chickens. A total of 61 kampong chickens used were used in the study including strain cobb, F1 of strain cobb-kampong, merawang, sentul, nunukan, and pelung, respectively. The method used is extracted DNA from blood samples, then amplified by Polymerase Chain Reaction (PCR) and then genotyping by DNA sequencing. The results showed that SNP was identified at position g.42988G> T in the intron 11 CAST gene of merawang chicken with genotype GG and GT. SNP was not identified in kampong chicken, strain cobb, F1 of strain cobb-kampong, sentul, nunukan, and pelung chicken, respectively. In conclusion, the intron 11 CAST gene region with 482 of product length of merawang chicken is polymorphic, whereas the other chicken species is monomorphic.   ABSTRAK Gen Calpastatin (CAST) merupakan salah satu gen yang berperan dalam proses keempukan pada daging. Gen CAST berfungsi sebagai inhibitor (penghambat) keempukan daging. Tujuan dari penelitian ini adalah mengidentifikasi Single Nucleotide Polymorphisms (SNP) gen CAST pada ayam kampung. Jumlah ayam yang digunakan 61 ekor ayam kampung, ayam strain cobb, F1 ayam strain cobb-kampung, ayam merawang, ayam sentul, ayam nunukan, dan ayam pelung. Metode yang dilakukan adalah ekstrasi DNA dari sampel darah, kemudian dilakukan amplifikasi menggunakan mesin Polymerase Chain Reaction (PCR) dan selanjutnya dilakukan genotiping melalui DNA sequencing. Hasil penelitian menunjukkan bahwa gen CAST intron 11 pada ayam pengamatan ditemukan SNP posisi g.42988G>T pada ayam merawang dengan genotip GG dan GT, sedangkan pada ayam kampung, ayam strain cobb, F1 ayam kampung dengan strain cobb, ayam sentul, ayam nunukan, ayam merawang dan ayam pelung tidak ditemukan SNP. Simpulan dari penelitian ini memperlihatkan gen CAST daerah intron 11 dengan panjang produk 482 pada ayam merawang bersifat polimorfik, sedangkan jenis ayam yang lain bersifat monomorfik.  
PENGARUH PENABURAN ZEOLIT PADA LANTAI LITTER TERHADAPPERSENTASE KARKAS DAN KOMPQN(EN NON KARKAS AYAM PEDAGING PADA KEPADATAN KANDANG YANG BERBEDA Kardaya, Dede; Ulupi, Niken
JURNAL PETERNAKAN Vol 2, No 2 (2005): September 2005
Publisher : JURNAL PETERNAKAN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Spreading zeolites on litter floor of broiler house at rate of 5.0 kg/m2 increased broiler carcass percentages up to 5.41 percents. Effect of zeolites-spread litters on broiler carcass and non-carcaSs components under different floor spaces had been performed in five weeks. As many as 324 chicks of a day-old Hubbard strain were fed with rations contained 3,000 -3,100 Kcal/I<g ME and 21 %crude protein during starter period and fed with the ration contained 20% crude protein duringfinisher period. The chicks were allocated to three different floor spaces (10, 12, and 14 heads/m2) and three different zeolites-spread litters (0.0, 2.5, and 5.0 kg zeolites/m2) under completely randomized design with factor 3 x 3 and 3 replications. Parameters concerned of present research including percentages of both carcasses and non-carcass components were analyzed with analysis of variance (ANOV A) and least .significant different.Results of the preseqt research showed that there was no interaction effect between floor spaces and zeolites-spread litters on all parameters concerned. Floor spaces affected the percentages of gizzard, kidney, and spleen significantly (p<O.05) but neither carcass nor abdominal fat percentage was af.fectedby the floor space. The zeolites-spread litters only affected significantly (P<O.05) on carcass percentage. Spreading zeolites at rates of5.0 kg/m2 on litter floor resulted in the highest carcass percentage (p<O.05) while rates of 25 kg/m2 resulted the same carcass pen:entage as the rates of 0.0 kg/m2. It was concluded that spreading zeolites at rates of 5.0 kg/m2 on litter floor under different floor spaces increased can:ass percentages up to 5.41 percents.
The Role of Triglyceride Lipase, Fatty Acid Synthase and Fatty Acid Binding Protein Family Genes on Fat Metabolism of Broiler Chickens Ulupi, Niken; Sumantri, C
WARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences Vol 25, No 1 (2015): MARCH 2015
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (285.385 KB) | DOI: 10.14334/wartazoa.v25i1.1124

Abstract

Broiler chicken has high fat content, especially abdominal and subcutan fat which reduced carcass quality and efficiency of feed energy. Genetic approach could be potentially applied to reduce high abdominal and intramuscular fat in broiler chicken through the selection program at gene level related to fat metabolism. This paper describes the metabolism and biosynthesis of body fat and the role of its controlled genes. Fat synthesis is controlled by feed quality and metabolism and biosynthesis process occurred in liver. These processes are controlled by many family genes, but certain genes have dominant role in the process; those are triglyceride lipase genes, fatty acid synthase genes and fatty acid binding protein genes. Expression of fatty acid synthase genes has positive correlation with fat content in liver and intramuscular. Expression of fatty acid binding protein genes was related to the increased abdominal fat deposit. These genes are polymorphic, so that they can be used as a genetic marker in selection to optimize feed efficiency, to minimize abdominal fat and to increase economic value of broiler chicken. Key words: Fat, triglyceride lipase genes, fatty acid synthase genes, fatty acid binding protein genes
IDENTIFIKASI KERAGAMAN GEN TOLL-LIKE RECEPTOR-4 PADA AYAM TOLAKI, AYAM PETELUR KOMERSIAL DAN AYAM BROILER ulupi, Niken
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 1, No 1 (2014)
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.169 KB)

Abstract

Gen Toll-like Receptor-4 (TLR4) adalah salah satu gen yang mengontrol ketahanan ayam terhadap Salmonella sp. melalui respons imun non spesifik. Gen tersebut dapat dijadikan sebagai marka genetik pada ayam apabila diketahui keragamannya. Oleh sebab itu tujuan penelitian ini adalah untuk mengidentifikasi keragaman gen TLR4 pada ayam Tolaki yang dibandingkan dengan ayam ras yang ada di Indonesia. Total sampel ada 126 terdiri dari ayam Tolaki (82 ekor), ayam petelur (20 ekor) dan ayam broiler (24 ekor). Tahapan identifikasi meliputi ekstraksi DNA genom, amplifikasi PCR gen TLR4 (220 pb, di exon 2), dan metode RFLP, menggunakan enzim restriksi MscI. Data yang dianalisis meliputi frekuensi alel dan frekuensi genotipe, nilai heterozigositas, dan Polymorphic Informative Content (PIC). Hasil yang diperoleh : gen TLR4|MscI pada ayam Tolaki, petelur dan broiler bersifat polimorfik, dengan ditemukan dua alel yaitu alel A dan G. Frekuensi alel G (pembawa sifat resisten) pada ayam Tolaki, broiler dan ayam petelur adalah 0.77, 0.87 dan 0.20. Dari uji x2 diketahui bahwa genotipe gen TLR4|MscI ayam Tolaki berada dalam keseimbangan Hardy-Weinberg, dengan nilai Ho dan He sebesar 0.31 dan 0.29. Nilai PIC gen TLR4 ayam Tolaki (0.25) termasuk kategori sedang. Berdasarkan hasil tersebut gen TLR4|MscI dapat digunakan sebagai penciri genetik untuk sifat ketahanan ayam terhadap infeksi Salmonella sp. pada ayam Tolaki.Kata kunci : keragaman genetik, gen TLR4, Salmonella sp, respons imun non spesifik
EFFECTS OF ZEOLITES ON LITTER FLOOR ON PERFORMENT OF BROILER Kardaya, Dede; Ulupi, Niken
Jurnal Zeolit Indonesia Vol 5, No 2 (2006)
Publisher : Jurnal Zeolit Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.556 KB)

Abstract

Effect of zeolites-spread litters on broiler performances under different floor spaces had been performed in five weeks. As many as 324 chicks of a day-old Hubbard strain were fed rations contained 3,000 – 3,100 Kcal/Kg ME and 21% crude protein during starter period and fed the ratio contained 20% crude protein with similar energy during finisher period. The chicks were randomly allocated to three different floor spaces (10, 12, and 14 heads/m2) and three different zeolites spread litters (0.0, 2.5, and 5.0 kg zeolites/m2) under completely randomized design with factor 3 x 3 and 3 replications. Parameters concerned of present research including feed intake, live weight gain, and mortality rate were analyzed with analysis of variance and least significant different. Results of the present research showed that there was no interaction effect between floor spaces and zeolites spread litters on all parameters concerned. Floor spaces affected both feed intake and feed conversion significantly (P
EFFECT OF ZEOLITES SPEREADING ON LITTERS TO BROILER PERFORMANCES REARED UNDER DIFFERENT FLOOR SPACES Kardaya, Dede; Ulupi, Niken
Jurnal Zeolit Indonesia Vol 5, No 1 (2006)
Publisher : Jurnal Zeolit Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.626 KB)

Abstract

Effect of zeolites spreading on litters to broiler performances under different floor spaces had been performed in five weeks. As many as 324 chicks of a day-old Hubbard strain were fed rations contained 3,000 – 3,100 Kcal/Kg ME and 21% crude protein during starter period and fed the ration contained 20% crude protein with similar energy during finisher period. The chicks were randomly allocated to three different floor spaces (10, 12, and 14 heads/m2) and three different zeolites spread-litters (0.0, 2.5, and 5.0 kg zeolites/m2) under completely randomized design with factor 3 x 3 and 3 replications. Parameters concerned of present research including feed intake, live weight gain, and mortality rate were analyzed with analysis of variance (ANOVA) and least significant different (LSD). Results of the present research showed that there was no interaction effect between floor spaces and zeolites spread litters on all parameters concerned. Floor spaces affected both feed intake (P
SEMEN QUALITY OF SENTUL COCK WITH DIFFERENT IMMUNOGLOBULIN YOLK CONCENTRATIONS Ariyanti, Ria; Ulupi, Niken; Suryati, Tuti; Arifiantini, Raden Iis
Buletin Peternakan Vol 43, No 4 (2019): BULETIN PETERNAKAN VOL. 43 (4) NOVEMBER 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.341 KB) | DOI: 10.21059/buletinpeternak.v43i4.37051

Abstract

The aim of this study was to evaluate the effect of different IgY concentrations on the quality of semen (macroscopic and microscopic) and testicular morphometry (weight, length and width of the testes) of Sentul cock testis and its ability to fertilize hens. This study used 4 months of 20 Sentul cocks consisting 10 high IgY concentration cocks and low IgY concentration cocks. This study used a complete randomized design (RAL) with 2 treatments namely low and high IgY concentration and10 times repetitions. Data were analyzed using t-test minitab program versions 16. Based on the result, different IgY concentrations did not affect the quality of semen and testicular morphometrics, but high IgY concentration cocks were able to fertilize more hens than the low IgY concentrations one by using dilution methods. This proves that high IgY concentration cocks are more effective and efficient cocks or stud.
A POLYMORPHISM OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 2 GENE ASSOCIATED WITH GROWTH AND BODY COMPOSITION TRAITS IN KAMPONG CHICKENS Furqon, Ahmad; Gunawan, Asep; Ulupi, Niken; Suryati, Tuti; Sumantri, Cece
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2018.19.2.183

Abstract

Insuline-like growth factor binding protein 2 (IGFBP2) merupakan protein pengikat utama yang memiliki fungsi biologi terlibat dalam pertumbuhan, perkembangan, dan diferensiasi. Program seleksi untuk sifat pertumbuhan cepat berdasarkan penanda molekuler sangat diperlukan untuk meningkatkan performa produksi. Terkait hal tersebut, penelitian ini didesain untuk menganalisis asosiasi keragaman gen IGFBP2 dengan sifat pertumbuhan dan komposisi tubuh pada ayam kampong. Ayam kampung merupakan ayam asli Indonesia dan tergolong ayam dengan pertumbuhan lambat. Sebanyak 59 ayam kampung jantan digunakan dalam penelitian ini. Pertumbuhan dan komposisi tubuh diukur pada umur 24 minggu. Sepasang primer didesain pada daerah intron 2 berdasarkan sekuen genom ayam. Sebuah SNP  c.1032C>T pada gen IGFBP2 intron 2 dideteksi dan metode PCR-RFLP digunakan dalam menentukan genotype ayam kampung. Hasil menunjukkan bahwa keragaman IGFBP2 signifikan berasosiasi dengan bobot badan, karkas, dada, otot dada, pectoralis minor, paha, dan sayap pada populasi ayam kampung (P<0.05). Penelitian ini menyarankan gen IGFBP2 dapat dijadikan kandidat gen yang mempengaruhi sifat pertumbuhan dan komposisi tubuh pada ayam.   Kata kunci: ayam kampung, pertumbuhan, komposisi tubuh, insuline-like growth factor binding protein 2, keragaman  
DETEKSI GEN Mx AYAM TOLAKI MENGGUNAKAN TEKNIK EKSTRAKSI DNA YANG BERBEDA Pagala, Muhammad Amrullah; Ulupi, Niken
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 1, No 1 (2014): JITRO, September
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2427.512 KB)

Abstract

Penelitian ini bertujuan untuk mencari metode yang efektif dalam teknik ekstraksi DNA dari gen Mx (Myxovirus) ayam Tolaki. Gen Mx adalah gen yang bertanggungjawab terhadap kemampuan ayam untuk mertespon serangan penyakit viral seperti penyakit Flu Burung dan New Castle Disesase. Penelitian ini dilaksanakan mulai bulan April sampai dengan bulan Agustus 2013.  Sampel penelitan terdiri atas 25 ekor ayam Tolaki dewasa, yang diperoleh dari pemeliharaan secara ekstensif oleh masyarakat di Kabupaten Konawe. Penelitian ini menggunakan kedua metode ekstraksi DNA yakni metode ekstraksi DNA secara konvensional dan metode Kit ekstraksi DNA.  Selanjutnya hasil ekstraksi DNA diamplifikasi menggunakan mesin PCR. Produk DNA kedua metode tersebut kemudian dibandngkan dan dianalisis secara kualitatif. Secara umum kedua metode tersebut cukup efektif untuk menghasilkan kualitas DNA gen Mx yang baik. Ekstraksi DNA gen Mx dari kedua metode tersebut menghasilkan ukuran DNA dengan panjang 299 pb.   Kata Kunci : Gen Mx, Ayam Tolaki, Ekstraksi DNA, PCR
IDENTIFIKASI KERAGAMAN GEN TOLL-LIKE RECEPTOR-4 PADA AYAM TOLAKI, AYAM PETELUR KOMERSIAL DAN AYAM BROILER Ulupi, Niken; Pagala, Muhammad Amrullah
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 1, No 1 (2014): JITRO, September
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4223.649 KB)

Abstract

ABSTRAK Gen Toll-like Receptor 4 (TLR4) adalah salah satu gen yang mengontrol ketahanan ayam terhadap serangan Salmonella sp, melalui respon immun non-specific. Gen ini dapat digunakan sebagai marka genetik pada ayam.  Oleh karena itu penelitian ini bertujuan mengevaluasi polimorfisme genetik gen TLR4 pada beberapa jenis ayam (Ayam Tolaki, Ayam Ras pedaging dan petelur). Total sampel sebanyak 126. Penelitian ini melalui 3 tahapan yakni ekstraksi DNA, amplifikasi PCR (dengan ukuran DNA 220 pb pada ekson 2), dan metode RFLP menggunakan  enzim MscI.  Hasil penelitian menunjukkan gen TLR4 | MscI bersifat polimorfik pada semua jenis ayam. Diperoleh 2 alel (A dan G). Frekuensi alel G (membawa sfat resisten) pada ayam Tolaki, Ras pedaging, dan petelur masing-masing 0.77, 0.87 dan 0.20. Nilaix2menunjukkan genTLR4|MscI berada dalan keseimbangan Hardy-Weinberg dengan Ho dan He 0,31 dan 0,29. Nilai PIC 0,25 termasuk kategori medium. Gen TLR4| MscI dapat digunakan sebagai marka genetik sifat ketahanan ayam Tolaki terhadap infeksi Salmonellasp.   Keywords : Polimorfik, Gen TLR4, Salmonella sp., Non-Specific Immune Response