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VALIDASI METODE FLOODING DOSE UNTUK MENGUKUR LAJU SINTESIS PROTEIN PADA SEL CACO-2 Ulyarti, Ulyarti
Journal of The Indonesian Society of Integrated Chemistry Vol 6 No 1 (2014): Journal of The Indonesian Society of Integrated Chemistry
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.34 KB) | DOI: 10.22437/jisic.v6i1.1937

Abstract

Metode flooding dose adalah salah satu teknik yang digunakan untuk mengukur laju sintesis protein.  Metode ini menggunakan asam amino yang diberi label radioaktif bersamaan dengan asam amino yang tidak berlabel radioaktif dalam jumlah yang besar.  Penelitian ini dilakukan untuk memvalidasi metode flooding dose yang digunakan untuk mengukur laju sintesis protein pada sel Caco-2. Penelitian ini berhasil menunjukkan tercapainya ekuilibrium antara aktifitas spesifik phenylalanine bebas intraseluler pada sel Caco-2 dan aktifitas spesifik phenylalanine bebas ekstraseluler pada media untuk menumbuhkan sel Caco-2.  Pencapaian ekuilibrium ini adalah validasi metode flooding dose untuk mengukur laju sintesis protein pada sel Caco-2. Kata kunci: flooding dose, laju sintesis protein, Caco-2
THE EFFECT OF TEMPERATURE ON THE PERFORMANCE OF ACTIVATED CARBON OVER CATALYTIC CRACKING OF CRUDE PALM OIL Nazarudin, Nazarudin; Ulyarti, Ulyarti; Alfernando, Oky; Galih, Ira; Susilawati, Susilawati; Doyan, Aris
Jurnal Penelitian Pendidikan IPA Vol 5, No 1 (2019): January
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v5i1.175

Abstract

This research was carried out to investigate the effect of temperature in carbon production on its performance in the catalystic cracking of CPO to fuel.  The carbon was produced using palm shell at 2 different temperatures (450 and 550oC).  The cracking of CPO was carried out with and without the active carbon catalyst.  The result showed that the use of catalyst increase the conversion of both gas and liquid conversion.  The use of higher temperature in the production of active carbon catalyst increased the performance of the catalyst, in particular, for the liquid conversion. Keywords :Activated carbon, catalyst, catalytic cracking, crude palm oil
STUDI SINTESIS KATALIS Cr/SiO2 DARI LIMBAH ARANG PABRIK KELAPA SAWIT SERTA UJI AKTIVITASNYA PADA PROSES PERENGKAHAN KATALITIK CRUDE PALM OIL (CPO) Nazarudin, Nazarudin; Bakar, Abu; Marlinda, Leny; Asrial, Asrial; Gusriadi, Dedi; Yani, Zunarta; Panda, Edy; Kanto, Rizky; Ulyarti, Ulyarti
Jurnal Ilmiah Ilmu Terapan Universitas Jambi|JIITUJ| Vol 1 No 2 (2017): Jurnal Ilmiah Ilmu Terapan Universitas Jambi
Publisher : LPPM Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.539 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui karakteristik katalis Cr/SiO2 untuk penggunaannya pada proses perengkahan katalitik CPO serta mengetahui aktivitas katalis Cr/SiO2 pada proses perengkahan katalitik CPO. Jenis penelitian yang dilakukan adalah penelitian eksperimen laboratorium, katalis hasil sintesis dianalisis dengan metode X-ray Difraktion dan SEM-EDS, katalis Cr/SiO2 hasil sintesis digunakan pada proses perengkahan katalitik CPO dengan memvariasikan jumlah CPO, perbandingan katalis:CPO (1:20;1:25;1:30). Bensin diperoleh memisahkan bensin dari Cairan Hasil Perengkahan (CHP) dengan cara destilasi. Hasil persentase konversi CHP yang diperoleh dari perengkahan katalitik Katalis:CPO (1:20;1:25;1:30) yaitu 58.53%;74.08%;58.76%. Sedangkan persentase konversi bensin pada perengkahan katalitik dengan perbandingan katalis:CPO (1:20;1:25;1:30) yaitu 9,91%;11,23%;8,09%. Arang dari limbah pabrik kelapa sawit dapat dijadikan sumber silika dan bisa disintesis menjadi katalis Cr/SiO2. 2. Katalis Cr/SiO2 sebagian besar mengandung SiO2 yang mempunyai sistem Kristal Hexagonal dan Cr/SiO2 mempunyai sistem Kristal Orthohombic. Namun demikian hasil sintesis katalis Cr/SiO2 pada penelitian ini kurang selektif untuk perengkahan katalitik CPO dibuktikan dengan peningkatan persentase konversi bensin yang tidak terlalu signifikan.
THE CHARACTERISTICS OF YAM (Dioscorea Alata) STARCH EDIBLE FILM Ulyarti, Ulyarti; Maryana, E.; Rahmayani, I.; Nazarudin, Nazarudin; Susilawati, Susilawati; Doyan, Aris
Jurnal Penelitian Pendidikan IPA Vol 5, No 1 (2019): January
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v5i1.174

Abstract

The purpose of this study was to determine the physical, mechanical and barrier properties of yam starch edible film. This research used Randomized Block Design (RAK).grouping the cultivar into 2, white and purple water yam. The starch concentration used were 1,33%, 2%, 2,67%, dan 3,33%.The results showed that the two cultivars produced similar properties of edible film.  The concentration of water yam starch affected transparency, thickness, water vapour transmision rate (WVTR) and compressive streght of edible film, but did not affect its solubility in water. The starch concentration of 2.67% was found to be the most appropriate concentration which produce edible film with thickness 0.12 to 0.13 mm, transparency 12.42 to 13.24%/mm, solubility 84.36 to 86.60%, water vapor transmission rate 1.16 x10-2 to 1.08 x10-2 g/sec/m2, and compressive strenght 48.10 to 49.35 N/m2Keywords:Edible Film, Water Yam, Cultivars, Starch concentration
Measuring Protein Synthesis Rate In Living Object Using Flooding Dose And Constant Infusion Methods Ulyarti, Ulyarti
Indonesian Food Science & Technology Journal Vol 1 No 1 (2017): VOLUME 1, Number (Issue) 1, December 2017
Publisher : Department of Food Science and Technology (THP), Faculty of Agricultural Technology, Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.406 KB) | DOI: 10.22437/ifstj.v1i1.4268

Abstract

Constant infusion is a method used for measuring protein synthesis rate in living object which uses low concentration of amino acid tracers. Flooding dose method is another technique used to measure the rate of protein synthesis which uses labelled amino acid together with large amount of unlabelled amino acid.  The latter method was firstly developed to solve the problem in determination of precursor pool arise from constant infusion method.  The objective of this writing is to compare the results from several researches on measuring protein synthesis rate using flooding dose and constant infusion methods and to obtain the criteria or prerequisite on the type of sample and tracer for each method. The measurements of protein synthesis rate using both methods are presented and results from both methods are compared.  Both methods are eligible for measuring protein synthesis rate as long as the true precursor can be measured.  The uncertainties in determining the precursor in constant infusion method and the effect of flooding on the stimulation of protein are the limitation on using the two methods. Bolus injection which imitates the flooding dose method but using smaller dose of tracer can be a good alternative in measuring protein synthesis rate.
Optimization of Anthocyanin Content in Uwi Flour (Dioscorea alata) Using Response Surface Methodology ulyarti, ulyarti
Indonesian Food Science & Technology Journal Vol 1 No 2 (2018): Vol 1 no 2, July 2018
Publisher : Department of Food Science and Technology (THP), Faculty of Agricultural Technology, Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1029.881 KB) | DOI: 10.22437/ifstj.v1i2.6006

Abstract

Abstract?Main problem in the processing of Dioscorea alata?s tuber into flour is the changing of colour.  One factor in color changing is attributed to antocyanin reaction in the tuber.  Several methods can be applied to minimise the browning and hence maintain the natural colour preferred by the consumers.  This research was conducted to obtain the combination of age of harvest, blanching temperature and blanching length which produce the highest content of antocyanin in Dioscorea alata?s flour. The responses measured was anthocyanin content. The result showed that age of harvest was the main factor for anthocyanin content of Dioscorea alata flour. The optimum anthocyanin content is predicted to be achieved by using tuber harvested at 9.6 months, temperature of blanching 86.12oC and blanching time for 0.56 menit.