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INTERACTION OF DIETARY PROTEIN AND ENERGY-PROTEIN RATIO ON GROWTH PERFORMANCE OF SNAKEHEAD CHANNA STRIATA Wijianti, Hani; Suprayudi, Muhammad Agus; Utomo, Nur Bambang Priyo; Jusadi, Dedi
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3451.682 KB) | DOI: 10.19027/jai.18.1.70-79

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ABSTRACTThe study proposed to discover the optimum protein content and energy-protein ratio in practical diet for snakehead, Channa striata. The experiment applied complete randomized design, consisted of six treatments with four replications on each treatment. The treatments were: A (32.5%; 11.82 kcal GE/g), B (32.5%; 13.32 kcal GE/g), C (37.17%; 10.64 kcal GE/g), D (37.33%; 12.07 kcal GE/g), E (43.08%; 10.02 kcal GE/g), and F (42.88%; 10.66 kcal GE/g). Snakehead with average weight of 6.21 ± 0.03 g were reared for 8 weeks. The treatments showed significant results. Increased protein dietary level had increased the growth rate and treatment with high energy-protein ratio at the same protein dietary level provided better growth performance. However, increased energy-protein ratio at E and F treatments caused a decline in growth performance. The improvement of energy-protein dietary ratio had positive correlation with the level of lipid and protein content in fish body. Similar finding was found in liver and muscle glycogen. The results also showed that the increament of energy-protein dietary ratio was significantly and positively correlated with cholesterol, triglyceride, HDL, glucose, and albumin, yet it had no significant effect on LDL. It can be concluded that excellent growth performance was achieved by protein dietary level of 37.33% and energy-protein ratio of 12.07 kcal GE/g.Keywords: energy-protein ratio, growth, snakehead ABSTRAKPenelitian ini bertujuan untuk memperoleh kadar protein dan rasio energi-protein pakan buatan yang optimum bagi performa pertumbuhan ikan gabus. Penelitian ini mengaplikasikan rancangan acak lengkap dengan 6 perlakuan, yaitu: A (32,78%;11,82  kkal GE/g), B (32,56%;13,32 kkal GE/g), C (37,17%:10,64 kkal GE/g), D (37,33%:12,07 kkal GE/g), E (43,08%:10,02 kkal GE/g), dan F (42,88%:10,66 kkal GE/g) dan masing-masing terdiri 4 ulangan. Bobot rata-rata ikan gabus yang digunakan dalam penelitian ini yaitu 6,21 ± 0,03 g dan dipelihara selama 8 minggu. Hasil penelitian menunjukkan bahwa peningkatan kadar protein pakan meningkatkan laju pertumbuhan, dan perlakuan dengan rasio energi-protein tinggi pada level protein pakan yang sama memberikan kinerja pertumbuhan yang lebih baik. Namun demikian, pada perlakuan E dan F, peningkatan rasio energi-protein menyebabkan penurunan kinerja pertumbuhan. Peningkatan rasio energi-protein pakan berkorelasi positif terhadap kadar lemak dan protein tubuh ikan. Hal yang sama juga terjadi pada glikogen hati dan otot. Hasil penelitian juga menunjukkan bahwa peningkatan rasio energi-protein pakan berpengaruh nyata (P<0,05) dan  berkorelasi positif terhadap kolesterol, trigliserida, HDL, dan albumin, tetapi tidak berpengaruh nyata terhadap LDL  (P>0.05). Kesimpulan yang didapat adalah kinerja pertumbuhan optimal dihasilkan oleh kadar protein pakan 37,33% dan rasio energi protein pakan 12,07 kkal GE/g.Kata kunci: albumin, energi, ikan gabus, pertumbuhan, protein 
ENHANCEMENT OF COLOUR QUALITY, GROWTH, AND HEALTH STATUS OF RAINBOW KURUMOI FISH MELANOTAENIA PARVA THROUGH DIETARY SYNTHETIC CAROTENOIDS SUPPLEMENTATION Meilisza, Nina; Suprayudi, Muhammad Agus; Jusadi, Dedi; Junior, Muhammad Zairin; Artika, I Made; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3529.969 KB) | DOI: 10.19027/jai.18.1.54-69

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ABSTRACT Carotenoids were known as pigment sources, the precursor of vitamin A, potential antioxidant and can improve the health status of fish. Furthermore, there are also studies that reveal the role of carotenoids in bone formation and metabolism. This study was conducted to determine the effect of different carotenoids at two different doses in the feed on growth, pigmentation, vitamin A conversion, blood profile, antioxidant activity, and calcium bone of the rainbow fish. Twenty-one aquariums with a volume of 20 L each stocked with 20 fish (1.08 ± 0.03 g of body weight and 4.56 ± 0.07 cm of body length). The experiment applied seven experimental diets (in triplicates) consisted of three types of carotenoids were astaxanthin (AS), canthaxanthin (CS), and lutein (LS) and two doses (130 and 260 mg/kg), i.e. AS-130, AS-260, CS-130, CS-260, LS-130, LS-260 and basal (without carotenoids) as the control. The fish were fed for 56 days of experimental period. The results showed that carotenoid diets were able to increase growth, total carotenoids, percentages of chromatophores, vitamin A conversion, erythrocyte, leukocytes, packed cell volume (PCV), neutrophils, and hemoglobin (Hb) compared to the control. Fish fed dietary astaxanthin at a level of 260 mg/kg was superior compared to other diets. Dietary carotenoids were also capable of decreasing the endogenous antioxidant activity of superoxide dismutase (SOD) and malonyl dialdehyde (MDA) and increased the calcium level in fish bone than basal diet. Keywords: carotenoids, growth, health status, Melanotaenia parva, pigmentation  ABSTRAK Karotenoid diketahui sebagai sumber pigmen, prekursor vitamin A, antioksidan potensial dan dapat meningkatkan status kesehatan ikan. Selain itu, karotenoid juga memiliki peran dalam formasi dan metabolism tulang. Penelitian ini dilakukan untuk mengevaluasi pengaruh jenis dan dosis karotenoid yang berbeda terhadap pertumbuhan, pigmentasi, konversi vitamin A, gambaran darah, aktifitas antioksidan dan kalsium tulang ikan rainbow Kurumoi. Sebanyak 20 ekor ikan (bobot tubuh rata-rata 1,08 ± 0,03 g dan panjang total rata-rata 4,56 ± 0,07 cm) dan diberi makan pakan yang mengandung karotenoid. Pakan uji terdiri atas tiga jenis karotenoid dengan tiga ulangan yaitu astaksantin (AS), cantaksantin (CS), dan lutein (LS) dan dua dosis (130 dan 260 mg/kg) dikodekan dengan AS-130, AS-260, CS-130, CS-260, LS-130, LS-260 dan basal (tanpa karotenoid) sebagai kontrol. Ikan diberi makan selama 56 hari pemeliharaan. Hasil penelitian menunjukkan bahwa karotenoid dapat meningkatkan pertumbuhan, total karotenoid, persentase kromatofora, konversi vitamin A, eritrosit, leukosit, hematokrit, neutrofil, dan hemoglobin dibandingkan dengan kontrol. Ikan yang diberi pakan mengandung astaksantin 200 mg/kg lebih baik dibandingkan denga pakan uji lainnya. Pakan yang mengandung karotenoid juga mampu menurunkan antioksidan endogenus superoxide dismutase (SOD) dan malonyl dialdehyde (MDA), serta meningkatkan kalsium tulang ikan dibandingkan pakan kontrol. Keywords: Melanotaenia parva, karotenoid, pertumbuhan, pigmentasi, status kesehatan. 
EFFECT OF ZINC (ZN) SUPPLEMENTATION ON QUALITY AND QUANTITY OF STRIPED CATFISH PANGASIANODON HYPOPHTHALMUS SPERM Kaliky, Nunun Ainun Putri Sari Banun; Setiawati, Mia; Carman, Odang; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 18 No. 1 (2019): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3415.809 KB) | DOI: 10.19027/jai.18.1.46-53

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ABSTRACT This study aimed to evaluate the effects of Zinc (Zn) supplementation on the quality and quantity of striped catfish sperm. Experimental design for this study was a complete randomized design with five treatments and five replications. Male broods fed with Zn supplementation for eight weeks. The Zn supplemented into the fish diet at different concentrations (0, 50, 100, 150 and 200 mg/kg of feed). The results showed that Zn supplementation could improve the quality and quantity of striped catfish sperm. The treatments also showed significant effects on semen volume, sperm motility, sperm viability, and sperm concentration (P<0.05). Zn supplementation at a dose of 200 mg/kg feed demonstrated the best result has indicated by enhancement of quality and quantity of striped catfish sperm, increasing 51% of the volume, 11.6% of motility, 5.81% of viability, 54.1% of concentrations. The results suggested that Zn played an important role in improving reproductive performances of male striped catfish reproduction. Keywords: quality of sperm, a quantity of sperm, striped catfish, supplementation zinc                                                                                            ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi Zinc (Zn) terhadap kualitas dan kuantitas sperma ikan patinPangasianodon hypophthalmus. Penelitian ini menggunakan rancangan acak lengkap dengan  lima perlakuan danlima ulangan. Induk jantan diberi pakan dengan suplementasi Zn selama 8 minggu. Zn disuplementasikan dengan dosis berbeda (0, 50, 100, 150 dan 200 mg/kg pakan). Hasil penelitian menunjukkan bahwa suplementasi Zn dapat meningkatkan kualitas dan kuantitas sperma ikan patin sehingga berpengaruh signifikan terhadap volume semen, motilitas, viabilitas dan konsentrasi sperma (P<0,05). Suplementasi Zn pada dosis pakan 200 mg/kg menunjukkan hasil terbaik yang ditunjukkan oleh peningkatan kualitas dan kuantitas sperma ikan patin 51% volume; 11,6% motilitas; 5,81% viabilitas; 54,1% konsentrasi sperma. Hasil penelitian ini menunjukkan bahwa Zn memainkan peran penting dalam meningkatkan reproduksi ikan patin. Kata kunci: kualitas dan kuantitas sperma, Ikan patin, suplementasi Zn 
UTILIZATION OF CINNAMON CINNAMOMUM BURMANNII LEAVES AND SHRIMP HEAD IN THE FEED ON GROWTH PERFORMANCE OF CATFISH PANGASIANODON HYPOPTHALMUS Dairun, Suclyadi; Setiawati, Mia; Suprayudi, Muhammad Agus; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 17 No. 1 (2018): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3324.195 KB) | DOI: 10.19027/jai.17.1.87-93

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ABSTRACTCatfish farming has confronted with the problem of slow growth to reach the fillet size and less tender of fish fillets. This study aimed to investigate the effect of cinnamon leaf flour and shrimp head flour in formulated diets on growth of catfish Pangasianodon hypopthalamus. Catfish with an initial weight of 207.04 ± 2.70 g/fish reared in 12 cages with a dimension of  2×1×1.5 m3 (L×W×H)  and its fish density was 15 fishes for 60 days. Fish were fed with following experimental diets: (1) Control; (2) cinnamon leaf (1%) (CL); (3) 45% of protein source from shrimp head (SH); (4) CL+SH mix.  The fishes fed twice a day with feeding rate 3.5% of fish biomass. The study found that the use of cinnamon leaf and shrimp head increased the specific growth rate 1.67-1.70%, the feed efficiency 57.55-57.67%, and protein retention 55.61% compared to its control (P<0.05). Triglyceride level, cholesterol, and blood HDL were 416.00-524.05 mg/dL, 139.65-156.68 mg/dL, 73.18-103.70 mg/dL (P>0.05), respectively. HSI value ranged between 0.3-1.9% compared to its control (P<0.05).Keywords: Cinnamomum burmannii, feed, growth, Pangasianodon hypopthalmus, shrimp head.  ABSTRAKBudidaya ikan patin dihadapkan pada permasalahan pertumbuhan dan kualitas daging yaitu pertumbuhan yang lambat untuk mencapai ukuran fillet serta tekstur daging kurang kompak. Tujuan penelitian ini adalah untuk menguji pengaruh penggunaan tepung daun kayu manis dan tepung kepala udang dalam pakan terhadap pertumbuhan ikan patin Pangasianodon hypopthalmus. Ikan patin dengan bobot 207,04 ± 2,70 g/ekordipelihara selama 60 hari di hapa sebanyak 12 buah (berukuran 2×1×1,5 m3)dengan padat tebar 15 ekor/jaring. Ikan diberi pakan perlakuan yang terdiri dari: (1) Kontrol; (2) daun kayu manis 1% (DKM); (3) 45% sumber protein dari kepala udang (TKU); dan (4) campuran DKM+TKU. Pemberian pakan sebanyak 2 kali sehari dengan feeding rate 3,5% dari biomassa ikan. Hasil dari penelitian penggunaan daun kayu manis dan kepala udang meningkatkan nilai laju pertumbuhan harian 1,67-1,70%, efisiensi pakan 57,55-57,67% dan retensi protein 55,61% dibanding kontrol (P<0,05). Kadar trigliserida, kolesterol, dan HDL darah berturut-turut adalah 416,00-524,05 mg/dL, 139,65-156,68 mg/dL, 73,18-103,70 mg/dL (P>0,05). Nilai HSI ikan patin berkisar antara 0,3-1,9% dibanding kontrol (P<0,05).Kata kunci: Cinnamomum burmannii, kepala udang, pakan, Pangasianodon hypopthalmus, pertumbuhan. 
THE GROWTH OF EEL FED WITH DIFFERENT PROTEIN LEVEL AND PROTEIN-ENERGY RATIO Nawir, Fitria; Utomo, Nur Bambang Priyo; Budiardi, Tatag
Jurnal Akuakultur Indonesia Vol. 14 No. 2 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3028.834 KB) | DOI: 10.19027/jai.14.128-134

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ABSTRACT The study was aimed to determine the optimum dietary protein level and energy protein ratio which can optimize growth performance of the eel A. bicolor bicolor on nursery phase.  Four treatments and three replications were applied in this study. The treatments were  P1, containing 37.66% protein with energy protein ratio 14.75 kcal GE/g (37.66%; 14.75 kcal GE/g), treatment P2 (41.30%; 13.51 kcal GE/g), treatment P3 (45.38%; 12.27 kcal GE/g), and treatment P4 (49.60%; 11.31 kcal GE/g). Eels used for this study were 6.5±0.3 g in average body weight. Eels were reared in a series of aquaria with dimension 90×40×40 cm3 and filled with 100 L of fresh water. Total weight of eel stocked in aquarium were 400 g. Eels were fed until satiated  twice a day at 8 am and 4 pm for 60 days. The result showed  that different protein level and energy protein ratio was significantly affected  growth performance (feed consumption, specific growth rate, feed efficiency, protein retention, and lipid retention), protein and fat of whole body eels at confident limit of 5%. In contrary, there was no significant different on the survival rate, hepatosomatic index, ash content, and nitrogen free extract of the body eel. The optimal growth performance was reached by dietary protein level and energy protein ratio of 45.38%; 12.27 kcal GE/g and 49.60%; 11.31 kcal GE/g. Keywords: Anguilla bicolor bicolor, energy protein ratio, feed, growth performance, protein  ABSTRAK Penelitian ini bertujuan menentukan kadar protein dan rasio energi protein optimum yang dapat meningkatkan kinerja pertumbuhan ikan sidat A. bicolor bicolor fase pendederan. Empat macam perlakuan dan tiga ulangan digunakan dalam penelitian ini. Perlakuan tersebut adalah P1 yang mengandung protein 37,66%; dengan rasio energi protein 14,75 kkal GE/g (37,66%; 14,75 kkal GE/g), perlakuan P2 (41,30%; 13,51 kkal GE/g), perlakuan P3 (45,38%; 12,27 kkal GE/g) dan perlakuan P4 (49,60%; 11,31 kkal GE/g). Bobot rata-rata ikan sidat yang digunakan adalah 6,5±0,3 g. Ikan sidat dipelihara dalam akuarium berukuran 90×40×40 cm3 dengan volume air 100 L. Total bobot ikan yang digunakan dalam setiap akuarium adalah 400 g. Ikan sidat diberi pakan sekenyangnya dengan frekuensi dua kali sehari yaitu pukul 08.00 dan pukul 16.00 WIB selama 60 hari. Hasil menunjukkan bahwa pemberian kadar protein dan rasio energi protein pakan berbeda, memberikan pengaruh nyata (P<0,05) terhadap kinerja pertumbuhan (jumlah konsumsi pakan, laju pertumbuhan spesifik, efisiensi pakan, retensi protein, retensi lemak), protein tubuh dan lemak tubuh, tetapi tidak berpengaruh nyata (P>0,05) terhadap tingkat kelangsungan hidup, indeks hepatosomatik, kadar abu, dan bahan ekstrak tanpa nitrogen (BETN) tubuh ikan sidat. Kinerja pertumbuhan optimal dicapai oleh kadar protein dan rasio energi protein pakan 45,38%; 12,27 kkal GE/g dan 49,60%; 11,31 kkal GE/g.  Kata kunci: Anguilla bicolor bicolor, kinerja pertumbuhan, pakan, protein, rasio energi protein 
THE GROWTH AND MEAT QUALITY OF SIAMESE CATFISH FED DIFFERENT LEVEL OF PROTEIN Purnomo, Novieanto; Utomo, Nur Bambang Priyo; Azwar, Zafril Imran
Jurnal Akuakultur Indonesia Vol. 14 No. 2 (2015): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3045.536 KB) | DOI: 10.19027/jai.14.104-111

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ABSTRACT This study was to evaluate growth performance and meat quality of Siamese catfish Pangasianodon hypophthalmus fed on commercial diets with different protein levels. Fish with average initial body weight of 33,61 g were reared in hapa (2×2×1 m3) at density of 30 ind/hapa. Fish were hand-fed with experimental diet to satiety twice daily for 60 days. Experimental design was set in completely randomized design. Each treatment was done in triplicates. Experimental diets were a commercial diet to contain four differennt levels of protein; i.e. diet A (18%), diet B (23%), diet C (28%), and diet D (32%). Specific growth rate (SGR), feed conversion ratio (FCR), protein (RP) and lipid (RL) retention, hepatosomatic index (HSI), lipid and glycogen content of liver, protein and lipid content of meat, edible portion, and fillet textural were calculated. The results of the experiment showed that the highest weight gain and SGR, lowest FCR and meat protein were obtained in the group of fish fed 23%?32% protein diets. In conclusion, 23% protein diets gave the best growth performance and meat quality of Siamese catfish. Keywords : growth performance, meat quality, commercial diet, Pangasianodon hypophthalmus  ABSTRAK Penelitian ini bertujuan untuk mengevaluasi kinerja pertumbuhan dan kualitas daging ikan patin siam Pangasianodon hypophthalmus yang diberi pakan komersial dengan kandungan protein berbeda. Ikan patin yang digunakan dengan bobot awal 33,61 g ditebar sebanyak 30 ekor/hapa ukuran 2×1×1 m3 dan ikan diberi pakan percobaan dua kali sehari secara at satiation selama 60 hari. Penelitian ini didesain dalam rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Pakan yang digunakan adalah pakan komersial dengan empat kandungan protein yang berbeda: pakan A (18%), pakan B (23%), pakan C (28%), dan pakan D (32%). Parameter uji pada penelitian ini adalah laju pertumbuhan spesifik (SGR), rasio konversi pakan (FCR), retensi protein (RP), retensi lemak (RL), indeks hepatosomatik (HSI), lemak dan glikogen hati, protein dan lemak daging, serta tekstur daging. Hasil penelitian menunjukan bahwa penambahan bobot tubuh, SGR tertinggi, FCR terendah, dan protein daging diperoleh pada perlakuan protein pakan 23%?32%. Hasil penelitian ini menunjukkan bahwa perlakuan protein pakan 23% memberikan kinerja pertumbuhan dan kualitas daging ikan terbaik. Kata kunci: kinerja pertumbuhan, kualitas daging, pakan komersial, Pangasianodon hypophthalmus
PEMBERIAN FIKOSIANIN SPIRULINA MENINGKATKAN JUMLAH SEL DARAH, AKTIVITAS FAGOSITOSIS, DAN PERTUMBUHAN IKAN KERAPU BEBEK JUVENIL (ADMINISTRATION OF SPIRULINA PHYCOCYANIN ENHANCES BLOOD CELLS, PHAGOCYTIC ACTIVITY AND GROWTH IN HUMPBACK GROUPER JUVENILE) Satyantini, Woro Hastuti; ., Sukenda; Harris, Enang; Utomo, Nur Bambang Priyo
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of this study was to investigate effects of Spirulina phycocyanin on the total  blood cell count,phagocytic activity, and growth of humpback grouper fish, Cromileptes altivelis juvenil.  Fishes were fedwith a diet containing   0, 150, 250, 350 dan 450 mg  phycocyanin per kg diet for four weeks and eachtreatment was triplicates.  Initial body weight  of  grouper was  8.46 ± 0.22 g with a density of 10 fish per56 litre volume. The total count of  erythrocytes and leucocytes increased until the fourth week of rearingperiod. The highest of total erythrocyte and leucocytes were observed in fish treated with 150 mg phycocyaninper kg diet ( 13.17 x  105 cells/mm3 and 8.93 x 105 cells/mm3 respectively) which were not significantlydifferent (P>0.05) to those treated with 250 mg phycocyanin per kg diet. The total leucocytes and phagocyticactivity of fish fed diet containing  250 mg phycocyanin  per kg diet (8.49 x 105 cells/mm3 and 59.67%respectively) were significantly higher  (P <0.05) to those of control group. The highest of final weight(Wt=14.32 g) and weight growth (G=5.89g) and lowest of feed conversion ratio (FCR=1.13) were obtainedin fish treated with  250 mg phycocyanin per kg diet which were  significantly  higher  (P <0.05) than thosefed control diet. The data showed that  the addition of  phycocyanin 250 mg/kg diet enhances the totalleukocyte count, phagocytic activity and the growth of humpback grouper juvenil.
EVALUATION OF INCUBATED DEFATTED RUBBER SEED MEAL WITH SHEEP RUMEN LIQUOR FOR PANGASIUS DIET Suprayudi, Muhammad Agus; Irawan, Winda Styani; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2822.981 KB) | DOI: 10.19027/jai.13.146-151

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ABSTRACT The research evaluated the use of rubber seed meal (Hevea brasiliensis; RBS) incubated with sheep rumen liquor as a subtitution of soybean meal in catfish Pangasionodon sp. diet. The fish was cultured for 40 days and fed with the experimental diet containing RBS at five different diet compositions regarding to soybean meal substitution level, i.e. 0% (control), 12%, 23%, 34%, and 44%. Feeding was done three times a day to satiation. No significant different was found on fish-protein retention and survival rate in all treatments. Based on the study result, the use of rubber-seed meal (Hevea brasiliensis; RBS) incubated with sheep rumen liquor could substitute soybean meal in catfish Pangasionodon sp. diet. Keywords: Hevea brasiliensis, Pangasionodon sp., catfish, sheep rumen liquor, rubber seed meal  ABSTRAK Penelitian ini mengevaluasi penggunaan tepung bungkil biji karet (Hevea brasiliensis; TBBK) yang diinkubasi dengan cairan rumen domba sebagai pengganti tepung bungkil kedelai pada pakan ikan patin Pangasionodon sp. Pemeliharaan ikan dilakukan selama 40 hari dengan pemberian lima komposisi pakan berbeda sesuai tingkat substitusi tepung bungkil kedelai oleh tepung bungkil karet. TBBK yang ditambahkan untuk mengganti bungkil kedelai adalah sebesar 0%, 12%, 23%, 34% dan 44%. Pemberian pakan dilakukan selama tiga kali sehari secara at satiation. Tidak ditemukan perbedaan signifikan (P>0,05) pada nilai retensi protein dalam tubuh dan kelangsungan hidup ikan uji pada semua perlakuan. Berdasarkan hasil penelitian, dapat disimpulkan bahwa tepung bungkil biji karet yang diinkubasi dengan cairan rumen domba dapat digunakan sebagai pengganti bungkil kedelai pada pakan ikan patin Pangasionodon sp. Kata kunci: Hevea brasiliensis, Pangasionodon sp., patin, rumen domba, tepung biji karet 
MASCULINIZATION OF NILE TILAPIA (OREOCHROMIS NILOTICUS) BY ADMINISTRATION OF BULL TESTES MEAL Muslim, Muslim; Junior, M. Zairin; Utomo, Nur Bambang Priyo
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.672 KB) | DOI: 10.19027/jai.10.51-58

Abstract

The synthetic steroid 17?-Methyltestosteron (MT) is commonly used as a feed additive to produce male population of Nile tilapia (Oreochromis niloticus). The use of synthetic testosterone hormone is not recommended in Indonesia. This study was conducted to evaluate the effect of natural testosterone hormone in bull testes meal (BTM) on the masculinization of Nile tilapia using validated aceto carmine squash method of gonads of the fish. Experimental design was utilized two factors experiments in completely randomized design. Fry kept in 40-L glass aquaria at a density of 40 fry/aquarium. Fry (7 dph) received the BTM  for 7 days (T1), 14 days (T2) and 21 days (T3) and doses 0% (D1), 3% (D2), 6% (D3), and 9% (D4).  When treatment was these results, indicated that significant (P?0.05) masculinization occurred only in the group treated of BTM and no treated of BTM. In the group treated of BTM, doses and duration treatment is not significant. The percentage of male fish 83.3% (9%-7d, 9%-21d, 6%-21d: doses and duration, respectively), higher than all group. Survival rate of fry (95-99.5%) is not affected by treatment BTM (no significant P?0.05). Fish growth was significantly affected by treatment BTM compare with no treated of BTM. The highest growth performance of fry were obtained with the 9% BTM.Key words: masculinization, nile tilapia, bull testes meal ABSTRAKSteroid sintetik 17?-Methyltestosteron (MT) umumnya digunakan sebagai aditif pakan untuk menghasilkan populasi ikan nila jantan (Oreochromis niloticus). Penggunaan hormon testosteron sintetis tidak dianjurkan di Indonesia. Penelitian ini dilakukan untuk mengevaluasi pengaruh hormon testosteron alami dalam makanan testis banteng (BTM) pada maskulinisasi ikan nila menggunakan metode divalidasi aceto carmine squash, dari gonad ikan. Desain eksperimental dimanfaatkan dua eksperimen faktor dalam desain benar-benar acak. Fry disimpan dalam 40-L akuarium kaca pada kepadatan 40 fry/akuarium. Fry (7 DPH) menerima BTM selama 7 hari (T1), 14 hari (T2) dan 21 hari (T3) dan dosis 0% (D1), 3% (D2), 6% (D3), dan 9% (D4). Ketika pengobatan hasil ini, menunjukkan signifikan (P ? 0,05) hanya terjadi maskulinisasi pada kelompok perlakuan dari BTM dan tidak diperlakukan BTM. Pada kelompok diobati BTM, dosis dan durasi pengobatan tidak signifikan. Persentase ikan jantan 83,3% (9%-7d, 9%-21d, 6%-21d: dosis dan durasi, masing-masing), lebih tinggi dari kelompok semua. Tingkat kelangsungan hidup benih (95-99,5%) tidak dipengaruhi oleh pengobatan BTM (tidak ada P yang signifikan ? 0,05). Pertumbuhan ikan secara signifikan dipengaruhi oleh BTM pengobatan dibandingkan dengan tidak diobati BTM. Kinerja pertumbuhan tertinggi fry diperoleh dengan BTM 9%.Kata kunci: maskulinisasi, ikan nila, tepung testis sapi 
FISH OIL SUPPLEMENTATION IN COMMERCIAL DIET ON GROWTH OF ANGUILLA BICOLOR BICOLOR Mukti, Retno Cahya; Utomo, Nur Bambang Priyo; Affandi, Ridwan
Jurnal Akuakultur Indonesia Vol. 13 No. 1 (2014): Jurnal Akuakultur Indonesia
Publisher : ISSA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2808.23 KB) | DOI: 10.19027/jai.13.54-60

Abstract

ABSTRACT This study was aimed to determine the effect of fish oil addition on growth performance and fatty acid composition of eel Anguilla bicolor bicolor. Freswater eel at an initial body weight of 9.90± 0.05 g were maintained in aquarium with volume of 120 L at stocking density of 1 g/L for 40 days. This research applied complete randomized design with four treatments addition of fish oil, i.e. 0%, 5%, 10%, and 15%. All treatments were carried out in triplicate. Feed given as much as 3% of the fish biomass a day for four times at 06:00, 11:00, 16:00, and 21:00. The results showed that the addition of fish oil in the diet have different effects (P<0.05) on specific growth (0.88?1.36%), feed efficiency (30.18?48.53%), protein retention (14.57?20.24%), fat retention (16.77?52.49%), energy retention (12.38?20.20%), and hepatosomatic index (1.72?2.72%) whereas the survival showed no difference (P>0.05) at 100%. In the fatty acid composition total of unsaturated fatty acid composition was 30.91?40.95%, n-3 fatty acids was 6.10?8.19%, and n-6 fatty acids were 6.18?8.19%. In conclusion, the addition of fish oil in the diet of freshwater eel Anguilla bicolor bicolor can be done up to 5% (13% fat content of diet). Keywords: Anguilla bicolor bicolor, fish oil, growth performance, fatty acid composition  ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh penambahan minyak ikan terhadap kinerja pertumbuhan dan komposisi asam lemak ikan sidat Anguilla bicolor bicolor. Ikan sidat dengan bobot 9,90±0,05 g dipelihara dalam akuarium dengan volume 120 L pada padat tebar 1 g/L selama 40 hari. Penelitian ini menggunakan rancangan acak lengkap terdiri atas empat perlakuan penambahan minyak ikan pada pakan sebesar 0%, 5%, 15%, dan 15%. Semua perlakuan terdiri atas tiga ulangan. Pakan diberikan sebanyak 3% dari biomassa ikan dan diberikan sebanyak empat kali sehari yaitu pukul 06.00, 11.00, 16.00 dan 21.00. Hasil penelitian menunjukkan bahwa penambahan minyak ikan dalam pakan memberikan pengaruh yang berbeda (P<0,05) terhadap spesific growth rate (0,88? 1,36%), efisiensi pakan (30,18?48,53%), retensi protein (14,57?20,24%), retensi lemak (16,77?52,49%), retensi energi (12,38?20,10%), dan indeks hepatosomatik (1,72?2,72%). Sintasan tidak menunjukkan adanya perbedaan (P>0,05) yaitu 100%. Pada komposisi asam lemak dihasilkan total komposisi asam lemak tidak jenuh 30,91? 40,95%, asam lemak n-3 6,10?8,19%, dan asam lemak n-6 6,18?8,19%. Dengan demikian, dapat disimpulkan bahwa penambahan minyak ikan dalam pakan ikan sidat Anguilla bicolor bicolor dapat dilakukan sampai dengan 5% (kadar lemak pakan 13%). Kata kunci: Anguilla bicolor bicolor, minyak ikan, kinerja pertumbuhan, komposisi asam lemak