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UJI PEMANFAATAN DAUN SIRSAK (ANNONA MURICATA L.) DALAM MENGHAMBAT STRES OKSIDATIF PADA TIKUS WISTAR HIPERKOLESTEROLEMIA MELALUI PENINGKATAN AKTIVITAS SUPEROXIDE DISMUTASE Wahjuni, Sri; Santi, Sri Rahayu; Astuti Wulandari, Ni Nyoman
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Oxidative stress is an imbalance condition between free radicals in the body with the anti-oxidant. Superoxide dismutase (SOD) is one of the endogenous anti-oxidant that can neutralize the excess of Reactive Oxygen Species (ROS). The excess of ROS that caused by stres oksidative can also be neutralized with the help of exogenous anti-oxidants. A soursop plant (Annona muricata L.) has anti-oxidant activity, especially in the leaves. This study aims to determine the activity of the leaves of the soursop (Annona muricata L.) in inhibiting the oxidative stress that conducted on the object hypercholesterolemic rats wistar through increasing SOD activity using variations of the extract dose of 50 mg/kg BW; 100 mg/kg BW and 150 mg/kg BW. In this study the dose gives the best result in inhibiting the oxidative stress through an increase in SOD activity was dose of 150 mg/kg BW with percent inhibition of SOD at (81,42% ± 3.91) where the percent inhibition of SOD is directly proportional to the activity of SOD
PENURUNAN ANGKA PEROKSIDA MINYAK KELAPA TRADISIONAL DENGAN ADSORBEN ARANG SEKAM PADI IR 64 YANG DIAKTIFKAN DENGAN KALIUM HIDROKSIDA Wahjuni, Sri; Kostradiyanti, Betty
Jurnal Kimia (Journal of Chemistry) Vol. 2, No. 1 Januari 2008
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Research on IR64 rice shell ash activation with KOH was carried out. Then, this activated ash known asadsorbent was applied to absorb H2O2 solution. This adsorbent was then applied to absorb peroxide number oftraditional coconut oil.The research includes activation of rice ash with 5%, 10%, and 15% KOH, followed by determination ofrate and isotherm adsorption towards H2O2. Finally, the ash was applied to absorb traditional coconut oil, in order todecrease peroxide number of the oil.Research results indicated that, adsorption rate of the ash with KOH activation is higher than without KOHactivation. Adsorption isotherm patern of the ash with and without activation is following Langmuir adsorption. Riceshell ash activated with 15% KOH can increased adsorption capacity of H2O2 about 297.16 mg/g, whereas withoutactivation the adsortion capacity is about 271.66 mg/g. Activated ash with 15% KOH was the applicated to decreaseperoxide number of traditional coconut oil. In this study, we obtain that the ash can decreased peroxide number until84.4%.
PERENCANAAN STRATEGIS LAYANAN PERPUSTAKAAN BERBASIS TEKNOLOGI INFORMASI STUDI KASUS DI PUSAT PERPUSTAKAAN DAN PENYEBARAN TEKNOLOGI PERTANIAN (STRATEGIC PLANNING OF LIBRARY SERVICES BASED ON INFORMATION TECHNOLOGY AT INDONESIAN CENTER FOR AGRICULTURAL LIBR Setyarini, Listina; Kusuma, Wisnu Ananta; Wahjuni, Sri
Jurnal Pustakawan Indonesia Vol. 16 No. 2 (2017): Jurnal Pustakawan Indonesia
Publisher : Perpustakaan IPB

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AbstractIndonesian Center for Agricultural Library and Technology Dissemination (ICALTD), as an institution library, has a function in providing information for supporting research and development in agriculture. Information technology has been used for supporting many library services at ICALTD. However, the implementation has not referred to a comprehensive and integrated strategic planning. A good strategic planning of information technology has an impact to the successful of utilizing information technology in the library. Therefore, this research aims to develop a strategic plan of IT-based library services at ICALTD based on the IT-IL V3 framework. The strategic assessment was conducted to analyze the external and internal factors through user surveys and interviews. The respondents were selected based on the organizational structure using SWOT analysis. Moreover, the survey results were analyzed using importance performance analysis (IPA) and user satisfaction index methods. In this research, several objectiveswas determined based on the importance and performance matrix diagram and the internal analysis results. In addition, this research recommended some improvements such as: 1) developing online catalog; 2) improving the library website; 3) developing library collections; 4) conducting user training for improving electronic services and 5) increasing access to internet. Keywords: Importance Performance Analysis, Iformation Technology, IT-IL V3, Library Services, Service Strategy, Strategic Planning, User Satisfaction Index
PENTAMETHYLCARBOXYLATE RUTHENOCENE BASED ANTITUMOUR AGENT Wahjuni, Sri; Puspawati, Ni Made; Williams, Michel
BALI MEDICAL JOURNAL Vol 4 No 2 (2015)
Publisher : BALI MEDICAL JOURNAL

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Background: Unlike iron ruthenium is not an essential element for life. However, the behavior of ruthenium compounds in biological systems and, in particular their use as antitumour agents has attracted much attention recently. This study aims to determine antitumour properties against human tumour cell line HeLa of pentamethylcarboxylate ruthenocene. Methods: This is an in vitro study by applying an experimental within post only control group design. Cisplatin, a clinical used medicine was applied for control. The human tumour cell line Hela was used for the test. The cells were cultured at 370C in 5% CO2/air in Roswell Park Memorial Institute Medium 1640 and seeded overnight in 96 well microtitre plates. The penthamethylcarboxylate ruthenocene was dissolved in 1,2 dimethoxy ethane and diluted with culture media. The plates were assays by measuring optical density in the range of 490-655 nm. The D37 values were then calculated. Results: The pentamethylcarboxylate ruthenocene compound tested was found to be more cytotoxic than cisplatin (with D37 = 422 nM compare to D37 = 705 nM for cisplatin). Conclusions: The compound tested, pentamethylcarboxylate ruthenocene was found more potent as an antitumor compare to cisplatin a clinical used antitumor for curing testicular carcinoma.
PEMBERIAN EKSTRAK ETANOL BUAH BUNCIS (PHASEOLUS VULGARIS L.) UNTUK MENURUNKAN KOLESTEROL TOTAL, LOW DENSITY LIPOPROTEIN (LDL) DAN MENINGKATKAN HIGH DENSITY LIPOPROTEIN (HDL) PADA TIKUS WISTAR DIET TINGGI LEMAK Wahjuni, Sri; Rustini, Ni Luh; Yuliantari, Putu
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 1 Januari 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Excessive fat consumption can increase blood cholesterol level. Phytosterol composition in the plant can decrease blood cholesterol level. One of the plants that contain phytosterol is beans. This study was to prove the effect of antihypercholesterol the ethanol extract of the beans (Phaseolus vulgaris L.) with variety of doses of 50 mg/Kg BW; 100 mg/Kg BW and 150 mg/Kg BW in male Wistar rats with high fat level condition. Subjects of this study were 24 Wistar rats divided into 6 groups randomly with posttest control group study design. First group as negative control, second group as positive control, and the third (high cholesterol diet and ethanol extract in dose of 50 mg/kg,bw), fourth ( high cholesterol diet and ethanol extractin dose of 100 mg/kg,bw), fifth ( high cholesterol diet and ethanol extract in dose of 150 mg/kg,bw),  sixth (high cholesterol diet and simvastatin drug). The data was analyzed with ANOVA Tukey test. The result of this study shows total cholesterol levels decrease 23,88%; 30,14%; 35,82%. Cholesterol LDL levels decrease 38,09%; 52,38%; 61,35%. Cholesterol HDL levels increase 21,86%; 56,56%; 63,84%. Dose variant on this study giving the best result to decrease total cholesterol levels and LDL was 150 mg/Kg BW. Furthermore, 100 mg/Kg BW dose gave the best result to increase HDL cholesterol level. Based on the results it can be suggested that beans ethanol extract is able to decrease total cholesterol level, LDL and increase HDL cholesterol levels.  
IMPLEMENTING MOBILE AGENT FOR LOCATION-BASED PRINTER SERVICES Saputra, Rendy Eka; Wahjuni, Sri
Jurnal Ilmu Komputer dan Informasi Vol 5, No 2 (2012): Jurnal Ilmu Komputer dan Informasi (Journal of Computer Science and Information)
Publisher : Faculty of Computer Science - Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1042.931 KB) | DOI: 10.21609/jiki.v5i2.192

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Most devices in ubiquitous network environment have limited memory and processing power, thus they canâ??t provide all services even when in suitable locations. Mobile agent technology could be implemented to improve resource efficiency. The goal of this paper is to implement mobile agent technology for location-based printer service. The system consists of four parts: (1) mobile agent server, (2) location-based service server, (3) printer service, (4) client. It is observed that mobile agent offers many advantages for ubiquitous and mobile computing settings. Perangkat dalam lingkungan ubiquitous memiliki memori dan daya proses yang terbatas, sehingga mereka tidak dapat menyediakan layanan secara utuh bahkan dalam lokasi yang tepat sekalipun.Teknologi mobile agent dapat diterapkan untuk meningkatkan efisiensi sumber daya. Tujuan dari paper ini adalah untuk melihat penerapan teknologi mobile agent pada lokasi berbasis layanan printer. Sistem ini terdiri dari empat bagian: (1) mobile agent server, (2) lokasi yang berbasiskan layanan server, (3) layanan printer, (4) klien. Hal ini menunjukkan bahwa mobile agent menawarkan banyak keuntungan untuk pengaturan komputasi ubiquitous dan mobile.
PEMANFAATAN MIKROORGANISME LOKAL BONGGOL PISANG DALAM PROSES FERMENTASI LIMBAH MAKANAN MENJADI PAKAN TERNAK Vikana Suari, Putu Primantari; Suyasa, I Wayan Budiarsa; Wahjuni, Sri
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 7 No 2 (2019): volume 7, Nomor 2, 2019
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: Penelitian ini bertujuan untuk mengetahui pengaruh mikroorganisme lokal (MOL) bonggol pisang terhadap proses dan hasil fermentasi limbah makanan menjadi pakan ternak, mengetahui dosis optimum MOL bonggol pisang dan lama waktu optimum MOL bonggol pisang pada fermentasi limbah makanan menjadi pakan ternak. Dalam penelitian ini digunakan  MOL bonggol pisang  sebagai starter dalam proses pengolahan limbah makanan menjadi pakan ternak. Pada tahap pertama, limbah makanan difermentasi dengan MOL pada dosis yang bervariasi, yaitu K5 (5mL), K10 (10 mL) dan K15 (15 mL). Fermentasi dilakukan selama 7 hari. Selama proses fermentasi dilakukan pengamatan  pH, suhu, warna, bau dan uji proksimat. Tahap kedua dilakukan penentuan waktu optimum  fermentasi dengan variasi waktu yaitu 3 hari, 7 hari, 10 hari dan 14 hari. Selanjutnya dilakukan fermentasi pada kondisi optimum yaitu dosis MOL 10 ml dan waktu fermentasi 7 hari dan hasil pengamatan  diolah dengan uji ANOVA. Hasil penelitian menunjukkan bahwa MOL bonggol pisang berpengaruh nyata (P<0.05) terhadap proses fermentasi dan kualitas pakan yang dihasilkan.   Kata kunci: Limbah makanan, Mikroorganisme lokal bonggol pisang,  fermentasi, pakan ternak   ABSTRACT: The aims of the research are to determine the effect of local microorganism (LMO) from banana hump on the process of fermentation of food waste to be able to use as animal food and to find out the optimum dose of banana hump LMO and the optimum time for the fermentation process. In this research, MOL of banana weevil was used as a starter in the process of processing food into feed. At the first time, food was fermented with MOL at varying doses, namely K5 (5mL), K10 (10 mL) and K15 (15 mL). Fermentation was carried out for 7 days. During the fermentation process the pH, temperature, color, odor and proximate tests were tested. The second step was determining the optimal time of fermentation with time variations of 3 days, 7 days, 10 days and 14 days. Furthermore, fermentation was carried out under optimal conditions, namely a 10 ml MOL dose and a fermentation time of 7 days and the observations were processed by ANOVA test. The results showed that the MOL of banana weevil proved significantly (P <0.05) on the fermentation process and the quality of the feed produced.
IDENTIFIKASI SENYAWA AKTIF ANTIMAKAN BIJI SIRSAK (ANNONA MURICATA L.) TERHADAP ULAT KUBIS (PLUTELLA XYLOSTELLA) Tabuni, Lusiana; Wahjuni, Sri; Ratnayani, Oka
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 2 Juli 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Identification of antifeedant active compounds from Soursop seeds (Annona muricata Linn) as natural pesticides against Plutella xylostella has been carried out. 1800 g Soursop seed powder  maceration was applied using methanol and n-hexane resulted in 46.42 g extract of methanol and 94.19 g extract of n-hexane. Viscous extract of methanol was diluted with the mixture of methanol:water (7:3), then evaporated until the entire methanol was removed, and after that it was partitioned with chloroform. The antifeedant activity of viscous extract of n-hexane, chloroform and water was tested which showed that the extract of n-hexane had highest activity compared to the others. Separation of 2 g viscous n-hexane extract with Coloumn Chromatography using Gel Silica 60 and n-hexane:aceton (4:1) as the mobile phase resulted in 5 fractions. The fractions were tested against Plutella xyllostella. The results showed that fraction C had the highest antifeedant activity. The isolate of fraction C was then identified using Gas Chromatography-Mass Spectrometry (GC-MS) based on the data base (Database\NISTO2.L and Database\wiley7n.1) suggested the active compounds were ethyl hexadecanoate ester and 11-octadecanoic acid.
Fabrication of Combined Prosthesis With Castable Extracoronal Attachments (Laboratory Procedure) Wahjuni, Sri; Mandanie, Sefy Ayu
Journal Of Vocational Health Studies Vol 1, No 2 (2017): November 2017
Publisher : Faculty of Vocational Education, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V1.I2.2017.75-81

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Background : Nows a days has developed many kind of dentures to improve the functionality, comfort aesthetics. Stability, retention and its aesthetic can be improved by attachments. With many kind design assortment of dentures so need a different technique with the existing design. Purpose : The aim of this study is to know the danture manufacturing process in combination with Castable protesa Extracoronal Attachments labolatory procedure. Review: Fixed dentures are each bonded teeth protesa, inserted mechanically and support by teeth or dental implant abutment or that provide the main support for the dental protesa. A partial denture is a spin-off protesa that replaces one or more missing teeth, on the maxillary or mandibullary and can be opened by patients without the supervision of a dentist. Extracoronal attachment is an attachment that is part of the male and female part are almost entirely outside the contours of the Crown of the tooth. Conclusion: the initial stages are making of coping use wax pattern and inserted the female, the casting of metal, porcelain applications, making the wax pattern of the metal frame work, the casting of metal, inserted male, making a removable dentures.
PEMBERIAN MINYAK IKAN LEMURU (SARDINELLA LONGICEPS) SEBAGAI ANTI DISLIPIDEMIA MELALUI PENINGKATAN HDL PADA TIKUS WISTAR Wahjuni, Sri
Jurnal Kimia (Journal of Chemistry) Vol. 5, No. 2 Juli 2011
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Changes in consumption behaviour to instant food can cause various health problems, such as obesity, dislipidemia, and atheroschlerosis. A study was conducted to investigate sardinella longiceps oil as anti dislipidemia through increasing HDL-C levels.This is a true experimental with randomized pre and posttest control group designs, emplyoying 50 Wistar rats grouped into five groups, i.e. control group (0% fish oil), treatment 1 group (10% fish oil), treatment 2 group (15% fish oil), treatment 3 group (20% fish oil), and treatment 4 group (25% sardinella longiceps oil). This research observed that intake of 20% sardinella longiceps oil resulted in the higest significant increase of HDL-C of 22.78 % (from 55.97 ±1.25 to 68.72 ± 6.25 mg/dL) for dislipidemia study. Therefore, it can be concluded that intake of sardinella longiceps oil in Wistar rat acts as anti dislipidemia by increasing the HDL-C levels. Further research is required to determine whether the application of sardinella longiceps on human will result in a simillar way, as anti inflammation and anti dislipidemia.