Sri Wahjuningsih
Laboratorium Reproduksi Fakultas Peternakan Universitas Brawijaya, Malang

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EFFECT OF ETHYLENE GLYCOL CONCENTRATION AND LENGTH OF EXPOSURE ON IN VITRO FERTILITY OF BOVINE OOCYTE Wahjuningsih, Sri; Hardjopranjoto, Suhartojo; Sumitro, Sutiman Bambang
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.813 KB) | DOI: 10.21157/j.ked.hewan.v4i2.3097

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The purpose of the study was to determine the influence of the concentration of ethylene glycol (EG) and length of exposure to levels of bovine oocytes in vitro fertility. This research was conducted using a completely randomized design factorial 5x3 with 7 replicates. The first factor was the concentration of cryoprotectants EG 10, 20, 30, 40, and 50%. The second factor was the length of exposure 1, 3, and 5 minutes. The results showed that the concentration of EG and the length of exposure were effect on fertilized oocytes (P 0.05). The level of oocytes in vitro fertility after vitrification in 30% EG and long exposure to 3 minutes did not different (P 0.05) compared to fresh oocytes, while the EG treatment 10, 20, 40, and 50% significantly showed a lower fertility rate than the 30% EG (P 0.05). It was concluded that the concentration of EG and length of exposure were effect on fertilized oocytes. The highest oocytes in vitro fertility level were found in 30% EG concentration with 3 minutes exposure time.
ULTRASTRUKTUR OOSIT KAMBING PASCA KRIOPRESERVASI DENGAN METODE VITRIFIKASI Wahjuningsih, Sri; Djati, Sasmito
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.079 KB) | DOI: 10.21157/j.ked.hewan.v7i2.915

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Tujuan penelitian adalah mengetahui ultrastruktur oosit pasca vitrifikasi menggunakan transmisi elektron mikroskop. Ovarium dikumpulkan dari rumah potong hewan dan oosit dikoleksi dengan cara aspirasi dari folikel menggunakan jarum ukuran 18 G. Setelah dilakukan klasifikasi kualitas  oosit,  maka  oosit  yang  berkualitas  A  dimaturasi  secara  in  vitro. Oosit   hasil  maturasi  dipaparkan  ke  dalam  larutan  vitrifikasi  yang mengandung 30%  EG  + 0,5 M  sukrosa  dengan  lama  waktu paparan  3 menit,  oosit  dimasukkan  ke dalam  ministraw  0,25 cc  (French  straw), kemudian dipaparkan pada uap nitrogen selama 10 detik dan dimasukkan dalam kontainer nitrogen cair. Setelah 3 minggu ministraw diambil dan dilakukan penghangatan (warming) di udara selama 10 detik, dimasukkan dalam waterbath suhu 35? C selama 30 detik. Isi ministraw dituangkan ke dalam cawan petri dan oosit dibilas dua kali dengan  sukrosa 0,5 M.  Selanjutnya dilakukan pengamatan ultrastruktur oosit dengan transmisi elektron mikroskop. Hasil penelitian menunjukkan oosit hasil vitrifikasi mempunyai zona pelusida yang abnormal (fraktur), mem bran plasma mengalami lisis, beberapa butir korteks mengalami degenerasi dan perpindahan butir korteks di ruang perivitelin. Kesimpulan dari penelitian ini adalah terdapat perubahan ultrastruktur pada oosit setelah vitrifikasi.
CYTOSKELETON OF BOVINE OOCYTES AFTER VITRIFICATION USING ETHYLENE GLYCOL CRYOPROTECTANT Wahjuningsih, Sri
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5023.182 KB) | DOI: 10.21157/j.ked.hewan.v2i2.9780

Abstract

The objective of this research is to know the influence of vitrification method toward thecondition of bovine oocytes cytoskeleton based on microtubules using the immunohistochemistrytechnique. Oocytes classified in two treatments such as fresh oocytes (control) and oocytes withvitrification treatments of 10, 20, 30, 40, and 50% of ethylene glycol (EG). Data were analyzed bydescriptive analyzing. The results of this research indicated that vitrification treatments were affected tomicrotubules organization of oocytes. Concentration of EG 10, 20, 40, and 50% of EG could not repaircytoskeleton after vitrification, but cytoskeleton structure could be protected by EG 30%.
THE EFFECT OF CENTRIFUGATION ON SEMEN QUALITY OF PERANAKAN ETTAWAH GOAT'S POST THAWING Indiah, Indiah; Wahjuningsih, Sri
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.034 KB) | DOI: 10.21157/j.ked.hewan.v4i2.9809

Abstract

The aim of the research was to determine the optimum of centrifugation on the quality of Peranakan Ettawah goat's spermatozoa prepared for in vitro fertilization preparation.The research of material was frozen semen ofPeranakan Ettawah goat produced by Artificial Insemination Center in Singosari with minimum post thawing motility in 40%. The method of the research was an experiment method with the four treatment, i.e: 1000 (P1), 1500 (P2), 2000 rpm (P3) of different centrifugation. The variable observes were spermatozoa motility, spermatozoa viability, and abnormal morphology of spermatozoa. Data obtain was analyzed statistically using Completely Randomize Design and continued with Tukey test. The result showed that on the upper layer of each treatment (P1, P2, and P3) obtained 55,72±3,55; 69,55±3,35; and 55,19±2,72% for spermatozoa motility, 62,99±5,87; 73,99±4,36; and 57,39±9,22% for spermatozoa viability, and 7,19±3,64; 8,84±2,65; and 6,40±3,00% for abnormal morphology of spermatozoa, descriptively. While on the lower layer the result showed 55,59±3,99; 68,63±3,88; and57,61±2,20% for spermatozoa motility, 70,05±7,47; 77,44±1,08; and 69,93±11,98% for spermatozoa viability, and 10,36±6,20; 9,55±3,27; and 8,09±2,80% for abnormal morphology of spermatozoa, desciptively. It was concluded that centrifugation in 1500 rpm showed the highest motility and viability on the upper layer and lower layer.
HUBUNGAN ANTARA TINGKAH LAKU SEKSUAL DENGAN PRODUKSI SPERMATOZOA SAPI BRAHMAN Sholikah, Nisa'us; Sutomo, Aris; Widiasmoro, Ndaru Pranowo; Wahjuningsih, Sri; Yekti, Aulia Puspita Anugra; Kuswati, Kuswati; Susilawati, Trinil
Jurnal Agripet Vol 18, No 2 (2018): Volume 18, No. 2, Oktober 2018
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.628 KB) | DOI: 10.17969/agripet.v18i2.12036

Abstract

ABSTRAK. Pejantan untuk program inseminasi buatan adalah pejantan unggul yang diseleksi berdasarkan libido dan produksi spermatozoa. Penelitian bertujuan untuk menganalisa hubungan antara tingkah laku seksual dengan produksi spermatozoa sapi Brahman. Materi penelitian adalah 3 pejantan sapi Brahman yang ditampung menggunakan vagina buatan. Metode penelitian adalah observasional dengan mengamati waktu reaksi dan lama ejakulasi. Observasi laboratorium dengan mengamati volume, konsentrasi, dan motilitas individu untuk menghitung total spermatozoa (TS) dan total spermatozoa motil (TSM). Pengamatan dilakukan sebanyak 10 ulangan. Data dianalisa secara deskriptif serta hubungan antara tingkah laku seksual dan produksi spermatozoa dihitung dengan korelasi Pearson. Hasil penelitian menunjukkan terdapat variasi tingkah laku seksual dan produksi spermatozoa pada sapi Brahman. Individu Cipta mempunyai waktu reaksi dan lama ejakulasi paling singkat serta produksi spermatozoa paling rendah. Hubungan signifikan antara waktu reaksi dengan TS serta TSM pada individu Bizzyard dengan koefisien determinasi (R2) sebesar 14 % dan 20%. Hubungan sangat signifikan antara lama ejakulasi dengan TS serta TSM pada individu Cipta dengan R2 sebesar 46% dan 58%. Kesimpulan penelitian adalah terdapat hubungan antara tingkah laku seksual dengan produksi spermatozoa pada sapi Brahman, dengan koefisien korelasi (r) tertinggi sebesar 0,72 dan R2 sebesar 58% antara lama ejakulasi dan total spermatozoa motil pada individu Cipta.  (The relationship between sexual behavior and semen production in Brahman bulls) ABSTRACT. Bulls for artificial insemination must be selected based on libido and sperm production. The study aimed to analyze the relationship between sexual behavior with sperm production on Brahman bulls. The material was 3 Brahman bulls collected using artificial vagina. The method was observational by observing reaction time and ejaculation duration. Laboratory observations by observing volume, concentration, and motility to calculate total sperm (TS) and total motile sperm counts (TMSC). Observations were replicated 10 times. The data were analyzed descriptively, the relationship between behavior and sperm production was examined using Pearson correlation. The results showed that there were variations in sexual behavior and sperm production in each bull. Cipta have the shortest reaction time and ejaculation duration and the lowest sperm production. A significant relationship between reaction time and TS and TMSC in Bizzyard with coefficient of determination (R2) of 14% and 20%. A very significant between ejaculation duration with TS and TMSC in Cipta with R2 of 46% and 58%. In conclusion, there was a relationship between sexual behavior and sperm production in Brahman bulls, with the highest correlation coefficient (r) of 0.72 and R2 of 58% between ejaculation duration and the total motile sperm counts in Cipta.
KONSENTRASI GONADOTROPIN RELEASING HORMONE (GNRH) EKSTRAK OTAK SAPI PERANAKAN FRIESIEN HOLSTEIN BETINA FASE FOLIKULER DAN LUTEAL Isnaini, Nurul; Wahjuningsih, Sri
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.998 KB) | DOI: 10.21157/j.ked.hewan.v8i2.2627

Abstract

Penelitian ini bertujuan mengidentifikasi kandungan gonadotropin releasing hormone (GnRH) ekstrak jaringan otak sapi dewasa pada fase folikuler (ovari berfolikel) dan fase luteal (ovari berkorpus luteum). Pada penelitian ini digunakan 2 buah otak sapi perah peranakan Friesien Holstein (FH) dewasa dengan umur 7-8 tahun yang pada saat disembelih kondisi ovarinya menunjukkan fase folikuler (1 buah otak) dan fase luteal (1 buah otak). Analisis kandungan GnRH dilakukan dengan metode enzyme-linked immunosorbent assay (ELISA) indirect. Hasil penelitian menunjukkan bahwa konsentrasi GnRH bagian otak sapi pada fase folikuler vs fase luteal berturut-turut untuk bagian otak parietal lobe of cerebral hemisphere, corpus callosum, thalamus, hipothalamus, pituitary gland (hipofise), midbrain, cerebellum, pons, dan medulla oblongata masing-masing adalah 260 vs 270, 110 vs 170, 230 vs 320, 1590 vs 1310, 840 vs 1250, 530 vs 810, 200 vs 480, 230 vs 100, dan 220 vs 70 ?g/ml. Dari hasil penelitian disimpulkan bahwa semua bagian otak sapi yang diamati mengandung GnRH dengan konsentrasi yang berbeda antara fase folikuler dengan fase luteal.
Antibody against progesterone in local rabbit following low dose of progesterone injection Suyadi, Suyadi; Wahjuningsih, Sri; Rachmawati, Achadiah; Srianto, P.; Pangestu, M.
Jurnal Ilmu-Ilmu Peternakan Vol 21, No 1 (2011)
Publisher : Fakultas Peternakan Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.117 KB)

Abstract

ABSTRACT: Antibody against progesgerone was produced from serum of local rabbit following low dose of progesterone injection: While a control group (Control; n=5) was injected with Freunds adjuvant solution in aquadest, the treatment groups were either firstly injected with progesterone conjugated to Freunds Adjuvant (P--CFA, 150 p.l : 150 pl) or progesterone conjugated to Freunds Adjuvant and bovine serum albumin (P-CFA-BSA; 135 p;l : 150 tt1 : 15 gl). Twice boostering injections were adminstered using incomplete Freunds Adjuvant on day 14 and 52 after first immunization. Weekly bleeding for serum collection were done from 1 week following first booster immunization to week 10, Using ELISA technique it was shown that the antibody titer to progesterone after first and second booster immunization in the P-CFA groug was higher than Control- and P-CFA-BSA groups. The antibody titer in the P-CFA-BSA remained low similar in the Control group:Key words: antibody; progesterone; rabbit
Analisis litter size, bobot lahir dan bobot sapih hasil perkawinan kawin alami dan inseminasi buatan kambing Boer dan Peranakan Etawah (PE) Kaunang, Dedy; Suyadi, S.; Wahjuningsih, Sri
Jurnal Ilmu-Ilmu Peternakan Vol 23, No 3 (2013)
Publisher : Fakultas Peternakan Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.106 KB)

Abstract

ABSTRACT:  The quality of semen inseminated to the dam might influence on the fer-tilization process and fetal growth during pregnancy. The aim of this research was to analysis the litter size, birth and weaning weight of Boer x PE goat crosses. Forty one parous and multiparous Peranakan Etawah (PE) does were naturally mated with selected Boer buck (NM-group), while the other 24 does were inseminated using artificial tech-nique using semen collected from the same buck as NM-group  and called as AI-group. The result showed no significant differences in litter size, birth weight and weaning weight between kids resulting from the does mated naturally and artificially insemina-tion technique (1.8±0.64 vs. 1.79±0.58; 2.93±0.568 vs. 2.96±0.397 kg; and 11.06 ± 2.001 kg vs. 11.31 ± 1.54 kg, for litter size, birth weight and weaning weight, respec-tively. It was concluded that mating technique in cross mating between PE does x Boer buck did not influence on the litter size and early growth phase of kids. Keywords: PE does, Boer buck, natural mating, artificial insemination
KUALITAS SEMEN SAPI LIMOUSIN PADA PENGENCER YANG BERBEDA SELAMA PENDINGINAN Bunga, Veronica Devita; Susilawati, Trinil; Wahjuningsih, Sri
TERNAK TROPIKA Journal of Tropical Animal Production Vol 15, No 1 (2014): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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Abstract

This study was done to evaluate quality of Limousin’s semen during chilled preservation. The characteristic of spermatozoa were easily damaged, so that they were  necessary to protect viability of spermatozoa. Addition of extender was conducted to determine quality and viability of spermatozoa. Each extender were added Egg Yolk as diluents cryopreservation, the treatment were devided in to three groups, they were ; P0 (CEP-2 + 10% Egg Yolk), P1 (Tris Aminomethane + 20% EggYolk), P2 (Skim + 10% Egg Yolk). The result showed that Tris Aminomethane + 20% Egg Yolk gave the best effect on maintaining quality of spermatozoa (motility, viability and abnormality). The motility in three extender at 24h was high significant different (P<0,01). Furthemore, the Viability and abnormality showed high significant different (P<0,01) since first time evaluation at 0h until 24h. Tris Aminomethane showed the best result among others due to the addition of 20% Egg Yolk, as we know that Egg Yolk was an extracellular cryoprotectan to protect and nourish spermatozoa during chilling preservation.   Keyword : Egg Yolk, CEP-2, Tris Aminomethane, Skim,
PENGARUH BANGSA PEJANTAN TERHADAP PERTUMBUHAN PEDET HASIL IB DI WILAYAH KECAMATAN BANTUR KABUPATEN MALANG Susanti, Indria; Ihsan, M Nur; Wahjuningsih, Sri
TERNAK TROPIKA Journal of Tropical Animal Production Vol 16, No 1 (2015): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.55 KB) | DOI: 10.21776/ub.jtapro.2015.016.01.7

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Penelitian ini dilaksanakan pada bulan Mei sampai Juni. Tujuannya adalah untuk mengetahui pengaruh bangsa pejantan Simmental dan Limousin terhadap pertumbuhan pedet hasil Inseminasi Buatan (IB) di Kecamatan Bantur, Kabupaten Malang. Materi yang digunakan dalam penelitian ini adalah pedet hasil IB dengan indukan Limpo yang disilangkan dengan pejantan Simmental 50 ekor dan Limousin 50 ekor pada umur 3−3,5 bulan. Metode yang digunakan dalam penelitian ini adalah survey secara purposive sampling. Data dianalisis dengan menggunakan analisis deskriptif dan uji beda. Hasil penelitian menunjukkan bahwa bangsa pejantan berpengaruh terhadap pertumbuhan pedet yang dihasilkan. Berbeda nyata pada bobot lahir, bobot badan 3-3,5 bulan, dan lingkar dada dari pejantan Simmental x Limpo berturut-turut adalah 35,46+2,86 kg; 134,42+54,61 kg; 120,74+10,64 cm, dan dari pejantan Limousin x Limpo  34,16+2,10 kg; 116,01+14,52 kg; 115+12,06 cm. Tidak berbeda nyata pada panjang badan dan tinggi gumba dari pejantan Simmental x Limpo berturut-turut adalah 99,02+8,81 cm; 105,38+10,13 cm, dan dari pejantan Limousin x Limpo 97,08+7,41 cm; 105,38+8,31 cm. Pedet hasil persilangan Simmental x Limpo memberikan performans produksi yang lebih baik dari pada Limousin x Limpo. Simmental merupakan salah satu pejantan yang berpengaruh cukup tinggi terhadap pertumbuhan pedet yang dihasilkan. Kata kunci: Pedet, Limousin, Simmental, Limpo, Performans Produksi