Djoko Wahyono
Department Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta Indonesia

Published : 99 Documents
Articles

IDENTIFIKASI POTENSI INTERAKSI OBAT PADA PASIEN RAWAT INAP PENYAKIT DALAM DI RSUD Prof. Dr. MARGONO SOEKARJO PURWOKERTO DENGAN METODE OBSERVASIONAL RETROSPEKTIF PERIODE NOVEMBER 2009 - JANUARI 2010 Sari, Andriana; Wahyono, Djoko; Raharjo, Budi
PHARMACIANA Vol 2, No 2: November 2012
Publisher : PHARMACIANA

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Abstract

Potensi interaksi obat adalah potensi aksi suatu obat diubah atau dipengaruhi oleh obat lain yang diberikan bersamaan. Interaksi obat didefinisikan sebagai fenomena yang terjadi ketika efek farmakodinamik dan farmakokinetik dari suatu obat berubah karena adanya pemberian obat yang lain. Interaksi obat dapat menyebabkan advers drug reaction apabila potensi terjadinya interaksi tersebut tidak diketahui sebelumnya upaya optimalisasi tidak dapat dilakukan. Tujuan penelitian ini adalah mengidentifikasi potensi interaksi obat pada pasien rawat inap penyakit dalam RSUD Prof. Dr. Margono Soekarjo Purwokerto. Metode penelitian adalah observasional retrospektif (November 2009 - Januari 2010) dengan menggunakan metoda deskriptif untuk analisa data. Hasil penelitian menunjukkan potensi interaksi obat pada pasien rawat inap penyakit dalam RSUD Prof. Dr. Margono Soekarjo Purwokerto sebesar 56,76% (n = 259). Berdasarkan kategori signifikansi yang dikemukakan Tatro (2006), terdapat potensi interaksi obat kategori signifikansi 1 pada pasien rawat inap penyakit dalam RSUD Prof. Dr. Margono Soekarjo Purwokerto sebesar 16,60%. Penggunaan obat berpotensi interaksi yang masuk kategori signifikansi 1 sebaiknya disesuaikan dengan kebutuhan pasien akan obat, risk and benefit serta dilakukan upaya optimalisasi.
EVALUASI PENGGUNAAN ANTIBIOTIK PROFILAKSIS TERHADAP KEJADIAN INFEKSI LUKA OPERASI PADA OPERASI SECTIO CAESAREA Oktaviani, Fifin; Wahyono, Djoko; Yuniarti, Endang
JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Vol 5, No 4
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1396.164 KB) | DOI: 10.22146/jmpf.217

Abstract

Infeksi nosokomial merupakan penyebab utama tingginya angka kesakitan dan kematian di dunia. Pada tahun 2002 CDC memperkirakan angka kejadian infeksi nosokomial di rumah sakit 1,7 juta orang dan sekitar 99.000 orang meninggal karena infeksi ini. Infeksi luka operasi (ILO) merupakan salah satu dari infeksi nosokomial mayoritas yang terjadi di rumah sakit. Penggunaan antibiotik profilaksis yang sesuai dapat mengurangi kejadian infeksi luka operasi. Penelitian ini bertujuan untuk mengetahui besarnya angka kejadian infeksi luka operasi pada operasi sectio caesarea, mengetahui pola dan kesesuaian penggunaan antibiotik profilaksis pada pasien sectio caesare adengan Pedoman Umum Penggunaan Antibiotik (PPAB) 2011, American Society of Health System Pharmacist (ASHP) Therapeutic Guideline 2012, Drug Information Handbook 22ndEdition. Metoda penelitian menggunakan metoda cross sectional melalui penelusuran data secara retrospektif terhadap rekam medik pasien yang menjalani tindakan operasi sectio caesarea di salah satu rumah sakit BUMN Kepulauan Riau periode Maret 2014 – Februari 2015. Analisis data berupa deskripsi angka kejadian infeksi luka operasi, pola dan kesesuaian penggunaan antibiotik profilaksis pada pasien sectio caesarea dengan pedoman. Hasil yang diperoleh dari penelitian ini menunjukkan bahwa besarnya angka kejadian ILO akibat tindakan sectio caesarea sebanyak 9 pasien (6,4%) dari jumlah total 140 pasien. Pola penggunaan antibiotik profilaksis menunjukkan bahwa penggunaan ceftriaxon sebanyak 55,7%, cefuroxim sebanyak 34,3%, kombinasi ceftriaxon dan metronidazol sebanyak 10%. Kesesuaian penggunaan antibiotik profilaksis menunjukkan bahwa rute dan interval pemberian sebesar 100% (140 pasien) sesuai dengan pedoman, sedangkan jenis obat yang digunakan, dosis, dan waktu pemberian masing-masing 34,3%, 65,7%, dan 72,9% yang sesuai dengan pedoman.Kata kunci: antibiotik profilaksis, infeksi luka operasi, sectio caesarea
COST OF ILLNESS PASIEN STROKE Purbaningsih, Sandi; Wahyono, Djoko; Suparniati, Endang
JURNAL MANAJEMEN DAN PELAYANAN FARMASI (Journal of Management and Pharmacy Practice) Vol 5, No 2
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jmpf.134

Abstract

Stroke adalah penyakit gangguan otak yang dapat mengakibatkan kematian dan kecacatan dengan biaya perawatan yang besar. Studi Cost of Illness (COI) bertujuan untuk mengukur beban ekonomi suatu penyakit. Penelitian dimaksudkan untuk melihat gambaran total biaya penyakit stroke, mengetahui perbedaan biaya riil pasien stroke rawat jalan dan rawat inap ditinjau dari faktor usia, jenis kelamin, kelas perawatan, lama perawatan, jumlah komorbid, cara bayar dan tipe stroke. Jenis penelitian adalah penelitian analitik dengan rancangan cross sectional berdasarkan perspektif rumah sakit. Pengambilan data dilakukan secara retrospektif melalui catatan medik pasien, bagian penjaminan, dan dari bagian Unit Teknologi Informatika di RSUP Dr. Sardjito Yogyakarta selama periode bulan Januari sampai Juni 2014. Variabel bebas meliputi usia, jenis kelamin, kelas perawatan, lama perawatan, jumlah komorbid, cara bayar dan tipe stroke meliputi stroke iskemik dan hemoragi, sedangkan variabel terikat adalah biaya riil pasien stroke rawat jalan dan rawat inap. Analisis data menggunakan analisis statistik deskriptif, Mann Whitney, dan Kruskal Wallis. Hasil penelitian menunjukkan bahwa total biaya penyakit stroke untuk 606 pasien adalah sebesar Rp. 5.867.281.315,97. Biaya rawat jalan menunjukkan berbeda signifikan ditinjau dari faktor usia, jenis kelamin dan cara bayar untuk stroke iskemik, sedangkan untuk stroke hemoragi menunjukkan beda signifikan ditinjau dari faktor jenis kelamin dan cara bayar. Biaya rawat inap menunjukkan beda signifikan ditinjau dari faktor usia, kelas perawatan, lama perawatan dan jumlah komorbid untuk stroke iskemik, sedangkan untuk stroke hemoragi menunjukkan beda signifikan ditinjau dari kelas perawatan, lama perawatan dan jumlah komorbid. Tipe stroke memberikan hasil perbedaan signifikan pada biaya stroke rawat jalan.
Profile of sulphacetamide pharmacokinetics on uranyl nitrate-induced renal failure rats Wahyono, Djoko; Hakim, Arief Rahman; Nugroho, Agung Endro
INDONESIAN JOURNAL OF PHARMACY Vol 18 No 3, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.141 KB) | DOI: 10.14499/indonesianjpharm0iss0pp117-123

Abstract

Kidney is a vital organ which has main function to maintain the body homeostasis. The role of kidney is the excretion (elimination) of waste product, and if there is pathologically a renal failure so will change the drug pharmacokinetics and in turn change it’s potency. The present study evaluated the effect of uranyl nitrate-induced renal failure on the pharmacokinetics profiles of sulphacetamide in rats.The study was conducted by employing a completely randomized design in male Wistar in bred rat (aged 2-2.5 months, 150-250 g). The animals in group I were given sulphacetamide sodium with an oral single dose 100.0 mg/kg BW (control group) and in group II were given pretreatment with uranyl nitrate at 3 days before sulphacetamide administration.After collected at some certain times, the drug concentrations on bloods were analyzed by an ultraviolet spectrophometer. The results have shown that uranyl nitrate-induced renal failure decreased the primer pharmacokinetics parameter i.e. total clearance (ClT) and volume of distribution in steady state (Vdss), significantly (P<0.05). The decreasing of these parameter could cause alteration of the secondary pharmacokinetics parameters of sulphacetamide and these derivates i.e. Cmax, tmax, AUC0-240, AUC0-inf, MRT, K and t1/2 elimination significantly (P<0.05). According from the results, it is concluded that uranyl nitrate-induced renal failure affected the pharmacokinetics of sulphacetamide or could increase the sulphacetamide concentration in blood.Key words : sulphacetamide, pharmacokinetics, renal failure, uranyl nitrate
THE USE OF GENERIC DRUGS IN PHARMACY IN THE MUNICIPALITY OF YOGYAKARTA DURING MONETARY CRISIS (OBSERVATION ON MARCH 1997 - MARCH 1998) Andari, Irin Dwi; Wahyono, Djoko
INDONESIAN JOURNAL OF PHARMACY Vol 12 No 4, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.501 KB) | DOI: 10.14499/indonesianjpharm0iss0pp12-20

Abstract

Monetary crisis that strike Indonesia had also impacts on health services either private or public services, including pharmaceutical industries. The industries had difficulty to find raw materials for production which in turn resulting in scarce and the rise of drug price. The situation could urged the patients to shift of using the patented medicines into generic ones which are relatively cheaper. The present study has therefore aimed to observe the usage of generic products before (March 1997 - August 1997) and during (September 1997 - March 1998) the monetary crisis periods in five pharmacies of Yogyakarta Municipality. The data were taken from the stocks units and from doctor’s prescriptions, which were then analyzed by ANAVA (p=0,5). Other data were also collected and analyzed descriptively from questionnaires requested to the a hundred of pharmacy’s visitors at the same periods as above.The results have shown that the number of generic product usage was not different between the two periods, except amoxycillin 500 mg, antalgin, dextrometorphan, captopril 25 mg, and glibenclamide tablets. The respondents have revealed that they have information on generic products (91%) from mostly mass media (44%). As many as 39 % of them assume that the generic products are cheaper than of patented products and it has the same effect.Key words : monetary crisis, generic product, pharmacies
Effect of the curcuma plus® syrup on the pharmacokinetics of rifampicin in rats Wahyono, Djoko; Hakim, Arief Rahman; ., Purwantiningsih
INDONESIAN JOURNAL OF PHARMACY Vol 18 No 4, 2007
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.119 KB) | DOI: 10.14499/indonesianjpharm0iss0pp163-168

Abstract

The research was aimed to observe the effect of Curcuma plus® syrup on rifampicin pharmacokinetics in rats.The study of interaction Curcuma plus® syrup with rifampicin pharmacokinetics was conducted employing a completely randomized design using male Sprague Dawley rats which were divided into 3 groups (5 rats for each group). The groups were given a single oral rifampicin dose of 50 mg/kg BW as a control group and were confered single oral Curcuma plus syrup 2.7 mL/kg BW one hour before rifampicin and daily dose for 7 days, then is given rifampicin after that. Serial blood samples (0,2 mL) were withdrawn at various interval via the vein for HPLC analysis of unchanged rifampicin in blood. The concentration of rifampicin was determined based on a standard curve, and from the concentration to time data was determined rifampicin pharmacokinetic parameters (Cmaks, tmaks, AUC0-∼, Vd/F, t1/2, ClT dan K).The results indicated that Curcuma plus® syrup single dose 2.7 mL/kg BW one hour before rifampicin could increased rifampicin volume of distribution by 225.80% (P<0.05) and caused decreased Cmaks 72.81% and AUC0-inf 63.93% (P<0.05), while daily dose Curcuma plus® syrup for 7 days could rise rifampicin total clearance 225.60% (P<0.05) and caused decrease by 76.94% of AUC0-inf (P<0.05).Key words : pharmacokinetics, Curcuma plus® syrup, rifampicin
The effect of pentagamavunon-0 pretreatment to the pharmacokinetic of paracetamol profile on wistar male rats Wahyono, Djoko; Hakim, Arief Rahman
INDONESIAN JOURNAL OF PHARMACY Vol 17 No 4, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.142 KB) | DOI: 10.14499/indonesianjpharm0iss0pp194-198

Abstract

Drug interaction can happen when two or more drugs are given together. This research was aimed to observe the effect of PGV-0 pretreatment to paracetamol pharmacokinetic profile in male rats.The study was conducted employing a one-way randomized completely design, using male Wistar rats weight 150 g (±10%). The animals were divided into three groups (5 rats for each group). Group I (control) was given a single oral paracetamol 150 mg/kg BW. The other groups II dan III were given a single oral PGV-0 20 and 40 mg/kg BW one hour before treatment with paracetamol respectively. After all rats were pretreated, serial blood and urine samples were withdrawn and were analysed using HPLC for unchanged paracetamol. Pharmacokinetic parameters of paracetamol i.e. Cmax, tmax, AUC, K, t1/2, Vdss/F, Cl/F, Aecum, dan %fe were determined based on concentration to time data in the blood and urine. The paracetamol pharmacokinetic parameters were analyzed by one-way analysis of varians (ANOVA) using 95% confidence interval. And the difference between groups were analyzed using Tukey-test method. The results showed that the pharmacokinetic parameters of paracetamol in the animals prefed with PGV-0 20 and 40 mg/kg BW did not change significantly (P > 0.05).Key words : pharmacokinetics, interaction, paracetamol, Pentagamavunon-0
The pattern of acute respiratory infections treatment in children of 0 – 59 month’s old in Puskesmas I Purwareja, Banjarnegara year of 2004 Wahyono, Djoko; Hapsari, Indri; Budi Astuti, Ika Wahyu
INDONESIAN JOURNAL OF PHARMACY Vol 19 No 1, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.403 KB) | DOI: 10.14499/indonesianjpharm0iss0pp20-24

Abstract

The purpose of this research was to describe the pattern of acute of respiratory tract infections in children of 0-59 month’s old in Puskesmas I Purwareja Klampok, Banjarnegara. The data was taken by retrospective method from medical record. The data collected was analysed by nonanalytic descriptive statistic method, and then compared them to standard of therapy provided by Indonesian Health Departement. The result showed that all of 120 patients were pneumonia acute respiratory tract infections, which were 55.8% boys and 44.2% girls. The antimicrobials used were Cotrimoxazol (86.7%) and amoxicillin (13.3%). The major product used in that puskesmas was syrup (91.7%), and tablet that delivered in the form of powder was 8.3%. The treatment using these antimicrobial at Puskesmas I Purwareja, Klampok, Banjarnegara was in accordance to standard of therapy provided by Indonesian Health Departement. Key words: pneumonia acute respiratory tract infection, 0-59 months old children, antimicrobial, puskesmas I Purwareja Klampok Banjarnegara
IDENTIFICATION OF THE VIRUS DENGUE–3 EPITOPE’S IMMUNODOMINAN USING THE SYNTHETIC PEPTIDE Nirwati, Hera; ., Sutaryo; Wahyono, Djoko
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.512 KB) | DOI: 10.14499/indonesianjpharm0iss0pp26-33

Abstract

Dengue virus infection has been known as an important health problem in many tropical countries, because of increasing number of patients, expansion of epidemic areas and emergence of severe clinical manifestations. Dengue virus consists of 3 structural and 7 nonstructural proteins. The major virion surface protein, the envelope protein, is the most important antigen with regards to virus biology and humoral immunity. Synthetic peptide derived from the envelope protein gene sequence can be used to identify region of the envelope protein that elicits antibodies. One hundred and sixty-one synthetic peptides were synthesized based on sequence of protein envelope dengue-3 virus published by Osatomi and Sumiyoshi. Each peptide consists of 15 amino acids with the last 12 amino acids overlapped. Peptide synthesizer, auto-spot robot ASP 222 (Abimed), was used for synthesis using solid spot method developed by Ronald Frank. Synthetic peptides attached to nitrocellulose membrane were used for spot immunoassay of 6 normal human sera, 6 dengue infected human sera and  6 non dengue-infected. Nine peptides were specific for dengue infected sera and these might be candidate immunodominat epitopes of dengue virus-3. They were EGLSGATWVDVVLEH (amino acids number 13-27), VCKHTYVDRGWGNGC (91-105),SIEGKVVQHENLKYT (124-138), TVHTGDQHQVGNETQ (142-156), TLGLECSPPTGLDFN (178-192), KGEDAPCKIPFSTED (325-339), PFSTEDGQGKAHNGR (334-348), GARRMAILGDTAWDF (406-420) and KIGIGVLLTWIGLNS (454-468). Based on spot immunoassay, 11 peptides were synthesized and used them for ELISA of 15 normal sera, 22 non-dengue infected sera and 37 dengue sera. Peptides BTLDDIELQKTEATQLA, BPFSTEDGOGKAHNGR and BKKEEPV NIEAEPPFG showed significantly different in their reactivities to the three groups. Combinations of two peptides were used for ELISA. Only one of them (BKGEDAPOKIPFSPED and BRMAILGD TAWDFGSV) showed significantly different in their reactivi- ties to the three groupsKey words: synthetic peptide, envelope protein dengue-3, spot immunoassay
A BIOEQUIVALENCE STUDY OF AMPICILLIN (GENERIC PRODUCTS) IN RABBITS Wahyono, Djoko; ., Nurlaila
INDONESIAN JOURNAL OF PHARMACY Vol 12 No 4, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.809 KB) | DOI: 10.14499/indonesianjpharm0iss0pp198-204

Abstract

The use of generic medicine in health services has strongly been suggested by government. Production of those type of medicine by government appointed pharmaceutical manufacturers has shown a good progress. However, monitoring to those products in the market, based on the bioequivalence quality, still need to be improved. An example of medicine produced in generic form is Ampicillin, one of the broad spectrum antibiotic effective to treat upper respiratory tract infections. The aim of this research was to evaluate the bioavailability of generic ampicillin capsules (500 mg) compared to the patented product. The research was carried out as follow: six male rabbits weighing 2.0 - 2.5 kg were assigned to a cross - over design to receive both products orally after 24 hour fasting. The generic and patented products were given to the rabbits with a week wash-out period. Following drug administration, the blood samples (11 samples) were drawn from the marginal ear vein at designated time (5-330 minutes) to analyse unmetabolized ampicillin fluorometrically. Detection of the ampicillin was recorded at maximum excitation (350 nm) and maximum emission (420 nm) wavelengths. The result showed that the AUC value of the generic product (3,211.25 ± 635.23 g hour/ml) was significantly higher than that of patented product (2,425.68 ± 895.26 g hour/ml) (p<0.05). The peak level of ampicillin (Cmax) was significantly higher for the generic (17.00 ± 5.10 g/ml) than patented product (11.25 ± 2.75 g/ml), while time to reach Cmax was not significantly different between the two products, ie. 77.34 ± 16.70 minutes (generic) and 82.48 ± 13.57 minutes (patented). The study concluded that the ampicillin capsules in generic form had a better biovailability than patented product. Key words: ampicillin, generic product, bioequivalence
Co-Authors Agung Endro Nugroho Andriana Sari Aningtyas, Tia Arief Rahman Hakim Bambang Sigit Riyanto Budi Raharjo Budiarti, L Endang Dyah, Ria Istamining Edi Prasetyo Nugroho Eka Purnomo Endang Suparniati, Endang Endang Yuniarti Endriastuti, Nialiana Endah Farida Hayati Fauziah Fauziah Fita Rahmawati Geografi, Liniati Harpina, Feranianty Haryati, Noor Hening Pratiwi, Hening Hera Nirwati, Hera Hidayati, Rahmania I Dewa Putu Pramantara Ika Puspita Sari Ika Puspitasari Ika Wahyu Budi Astuti, Ika Wahyu Ilma, Dewi Latifatul Indri Hapsari Irin Dwi Andari, Irin Dwi Kartikarini, Victoria Dian L. Endang Budiarti, L. Endang Laksmi Maharani Manik, Nirmala Megawati, Sefi Mulyasih, Anita Budi Mustofa Mustofa Nanang Munif Yasin Naniek Widyaningrum Ninisita Sri Hadi Norcahyanti, Ika Nova Hasani Furdiyanti, Nova Hasani Nurlaila ., Nurlaila Nutrisia Aquariushinta Sayuti, Nutrisia Aquariushinta Oktaviani, Fifin Oktianti, Dian Pangestuti, Titi Ira Prabandari, Yayi S. Pramantara, Dewa Putu Pratiwi, Daniar Purbaningsih, Sandi Purwaningsih, Avianti Eka Dewi Aditya Purwantiningsih ., Purwantiningsih Putri, Dianita Rifqia Rahmantika, Fitri Retno Wahyuningrum Riyanto, Bambang S. Rizka Humardewayanti Asdie, Rizka Humardewayanti Rukminingsih, Fef S, I Dewa Putu Pramantara Sampurno Sampurno Sandi, Dita Ayulia Dwi Sari, Ika P. Sari, Lana Satibi Satibi Suci Paramitasari Syahlani Sukarno, Ristantio Susi Ari Kristina Sutaryo ., Sutaryo Tambunan, Veny Titik Nuryastuti, Titik Tommy Pratama, Tommy Tri Murti Andayani Umboro, Recta Olivia Wati, Helmina Wulandari, Denia Yuni Yasin, Nanang M. Yasin, Nanang M. Yayi Suryo Prabandari Yayuk Dwi Rahayu, Yayuk Dwi Yuhansyah Nurfauzi Yusmein Uyun, Yusmein