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Journal : Oseanologi dan Limnologi di Indonesia

Diversity of Culturable Actinomycetes from Deepsea Floor of Makassar Strait, Indonesia Hatmanti, Ariani; Lisdiyanti, Puspita; Widada, Jaka; Wahyuono, Subagus
Oseanologi dan Limnologi di Indonesia Vol 3, No 2 (2018)
Publisher : Oseanologi dan Limnologi di Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/oldi.v3i2.205

Abstract

With regard to collaboration research called Widya Nusantara Exploration (EWIN) in May-June 2013 and November 2014, a study on isolation of actinomycetes from sediments of Makassar Strait have been conducted. Actinomycetes is one of microbe which has an excellent track record in producing antimikrob and other active substances. But due to terrestrial actinomycetes has been widely explored, then recently researchers began focusing on wide variety of extreme environments, such as marine environment, to screening aktinomisetes in producing new secondary metabolites. A total of 36 strains of actinomycetes were isolated from 10 samples obtained from deepsea floor in Makassar Strait, Indonesia, Direct Dillution Method were best used to isolate the actinomycetes compare to Sodium Dodecyl Sulfida – Yeast Extract Method (SDS-YE Method) and Rehidration Centrifugation Method (RC Method). NBRC-802 media and Actinomycetes Isolation Agar(AIA)(Himedia)media were used as the isolation media. All the isolates were identified by morphological characteristic and by analysis of 16S rRNA gene sequence. Actinomycetes isolated from deepsea floor of Makassar Strait have been dominated by Micromonospora (58%), Verrucosispora (14%)Streptomyces (8%) and Luteipulveratus (5%), however genus Nocardiopsis, Micrococcus, Gordonia, Kytococcus, and Arthrobacter were not dominant (3%). Station 25 in 1.547 m depth was the most abundant of actinomycetes, 18 strains and dominated by the genus Micromonospora which is isolated using Direct Dillution Method and both NBRC 802 or AIA media.
Co-Authors Abdul Karim Zulkarnain Abdul Rohman Aditya Krishar Karim Ag. Yuswanto Agung Endro Nugroho Agustinus Yuswanto, Agustinus Ahmad Hamim Sadewa Ambarwati Ambarwati Ari Sartinah, Ari Ariani Hatmanti Arnida ., Arnida AWM. Boersma, AWM. B.S. Ari Sudarmanto, B.S. Ari Bambang Hariono Citra Prasetyawati, Citra Dinar Sari Djoko Santosa Djoko Santoso Ekowati Chasanah Endang Dwi Wulansari Endang Lukitaningsih Erna Prawita Setyowati Farisya Nurhaeni Fauziati, Vera Galih G, Galih Galih, G. Gemini Alam Hadi, Etik Erna Wati Hari Susanti HERNAYANTI HERNAYANTI Hosea Jaya Edy Ibnu G Gandjar, Ibnu G Ibnu G. Gandjar, Ibnu G. Ibnu G. Ganjar, Ibnu G. Ika Puspita Sari Imono A. Donatus, Imono A. Imono Argo D, Imono Imono Argo Donatus, Imono Argo Indah Purwantini Isnindar Isnindar, Isnindar JAKA WIDADA K. Nooter, K. Kaneko, Takushi Kazutaka maeyama Kumiko Terada KV Rao, KV L. Hartanto Nugroho LANGKAH SEMBIRING Linus Yhani Chrystomo Lukman Hakim Mae S.H. Wahyuningnsih, Mae S.H. Mae S.H. Wahyuningsih Mae Sri Hartati W, Mae Sri Mae Sri Hartati Wahyuningsih, Mae Sri Hartati Mae Sri Hartati, Mae Sri Mahardika, Arfian Bela Marchaban ., Marchaban Marchaban Marchaban, Marchaban Marchaban, . Marchaban, . Mark T Hamann, Mark T Mark T Hamman Mark T. Hamann, Mark T. Mimiek Murrukmihadi Moch. Saiful Bachri Mohd., Datin Maryati mufrod mufrod Muhammad Nursid Murwantoko Murwantoko Mustofa . Nanang Fakhrudin, Nanang Nunung Yuniarti Nur Hidayati Nuringtyas, Tri R. Nurlaila Nurlaila Peni Susilowati Putri, Peni Susilowati Perdana Priya Haresmita, Perdana Priya Pratiwi, Sylvia Puji Astuti Purwantiningsih, Purwantiningsih PUSPITA LISDIYANTI R.L.M. Bolhuis, R.L.M. Rarastoeti Pratiwi Ratna Asmah Susidarti Retno Wahyuningrum Rijai, Hifdzur Rashif Ritmaleni Ritmaleni RR. Endang Lukitaningsih S.M. Widyastuti Sakti, Dika Sotya Samsul Hadi Sebastian Margino Sita Rina Widyarini, Sita Rina Sitarina Widyarini Soesanto Tjokrosonto Sofia Mubarika Sorta Basar Ida Simanjuntak Sri Sudewi, Sri Sri Wahdaningsih, Sri Sudarsono . Sudibyo Martono Sugeng Riyanto Sukarti Moeljopawiro Sutomo Sutomo suwaldi suwaldi Syamsudin . Tamhid, Hady Anshory Tanti Azizah S Tanti Azizah Sujono Tatang Irianti Tatsuo Takeya, Tatsuo Titik Nuryastuti, Titik Tri Lestari Tri R Nuringtyas, Tri R Triana Hertiani Tsutomu Nohno Vairappan, Charles S. Wahyono Wahyono Wahyuningsih, Mae Sri Hartanti Wayan Tunas Artama Widyastuti, S M Widyastuti, SM Widyastuti, SM Widyastuti, SM Yoga Pratama, Yoga Yuli Widiyastuti Yustina Sri Hartini, Yustina Sri Yuswanto Yuswanto Yuwantiningsih, Sri