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Journal : JFIOnline

PENGARUH EKSTRAK AKAR SENGGANI (Melastoma affine D.Don) TERHADAP MOTILITAS DAN JUMLAH SPERMA TIKUS Bachri, Moch. Saiful; Donatus, Imono Argo; Wahyuono, Subagus
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 4, No 2 (2008)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Spermatogenesis effect of  ethanol extract senggani root (Melastoma affine D. Don) was proved. Furthermore, the extract was fractionated with ethyl acetate and was investigated toward spermatogenesis effect to explore natural product  as male  contraception. The subject of this research were five groups of thirty two fertile male Wistar rat,. Four groups were treated with ethyl acetate fraction of senggani root in gradually 500; 250; 125; and 62,5 mg/kg body weight dose. The fifth group as negative control was treated by CMC Na 0,5 %. Every groups was treated by orally administration for twenty two days period of time, once a day. In twenty third day, the rats were killed then observed toward the motility, sperm count, and testis histopathology. Results of the research indicated that there were influence toward spermatogenesis effect from ethyl acetate fraction of senggani root, especially at sperm motility and sperm count. Decrease of sperm motility after treatment was compared with negative control group. The percentage of the sperm count decreased at 500; 250 mg/kg body weight dose from ethyl acetate fraction was 49,74 % and 48,21.This decreased probably were caused by seminiferous tubules necrotic.   ABSTRAK Pemberian ekstrak etanol akar senggani (Melastoma affine D. Don) telah terbukti dapat menyebabkan berkurangnya jumlah dan motilitas sperma tikus. Berdasarkan hasil penelitian tersebut dilakukan fraksinasi ekstrak etanol akar senggani dengan etil asetat kemudian fraksi itu di uji cobakan pada tikus dengan tujuan untuk eksplorasi bahan alam yang dapat dipakai sebagai bahan kontrasepsi pria. Penelitian ini menggunakan 32 ekor tikus jantan fertil galur Wistar, umur 2,5-4 bulan dengan bobot 145-350 g dan dibagi menjadi 5 kelompok. Empat kelompok merupakan kelompok perlakuan fraksi etil asetat akar senggani dengan dosis 500; 250; 125; dan 62,5 mg/kgBB. Kelompok V merupakan kelompok kontrol negatif dengan perlakuan CMC Na 0,5 %. Semua kelompok diberi perlakuan secara per oral selama 22  hari dengan frekuensi pemberian sekali sehari. Pada hari ke-23 tikus dikorbankan dan diamati motilitas, dan jumlah atau angka sperma. Dilakukan juga pengamatan histopatologi organ testis. Hasil penelitian menunjukkan ada pengaruh pemberian fraksi etil asetat akar senggani terhadap motilitas dan jumlah (angka) sperma. Motilitas sperma menjadi berkurang pada semua dosis perlakuan jika dibandingkan dengan kelompok kontrol. Penurunan motilitas sperma terbesar pada dosis 250 mg/kgBB. Jumlah sperma juga mengalami penurunan untuk semua dosis perlakuan. Persentase penurunan jumlah sperma terbesar pada dosis 500 ; 250 mg/kgBB sebesar 49,74 % , dan 48,21 %. Penurunan ini kemungkinan diakibatkan oleh rusaknya tubulus seminiferus testis yang mengalami nekrosis
Penetapan Kadar Alkaloid Ekstrak dari Etanolik Bunga Kembang Sepatu (Hibiscus rosa-sinensis L.) Murrukmihadi, Mimiek; Wahyuono, Subagus; Marchaban, .; Martono, Sudibyo
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 3 (2013)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.643 KB)

Abstract

Kembang sepatu flower (Hibiscus rosa-sinensis L.) was fractionally used as expectorant. Based on Bioassay Guided fractionation, an active fraction was separated, and the fraction was identified is Alkaloid was the major compound based on TLC analysis. Viscosity value measured by viscometer was used as a Bioassay model of expectorant activity in vitro and acetyl cysteine was used as positive control. Alkaloid content determination of the ethanolic extract was measured by TLC-Densitometric compared with standard curve of isolated alkaloid as the selected marker (Y=12,1360X+2901,4474). The alkaloid content in the ethanolic extract was determined as 2.35±0,67%.Keywords : alkaloid, ethanolic extract, Hibiscus rosa-sinensis L.
PENGARUH EKSTRAK AKAR SENGGANI (Melastoma affine D.Don) TERHADAP MOTILITAS DAN JUMLAH SPERMA TIKUS Bachri, Moch. Saiful; Donatus, Imono Argo; Wahyuono, Subagus
Jurnal Farmasi Indonesia Vol 4, No 2 (2008)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v4i2.15

Abstract

Spermatogenesis effect of  ethanol extract senggani root (Melastoma affine D. Don) was proved. Furthermore, the extract was fractionated with ethyl acetate and was investigated toward spermatogenesis effect to explore natural product  as male  contraception. The subject of this research were five groups of thirty two fertile male Wistar rat,. Four groups were treated with ethyl acetate fraction of senggani root in gradually 500; 250; 125; and 62,5 mg/kg body weight dose. The fifth group as negative control was treated by CMC Na 0,5 %. Every groups was treated by orally administration for twenty two days period of time, once a day. In twenty third day, the rats were killed then observed toward the motility, sperm count, and testis histopathology. Results of the research indicated that there were influence toward spermatogenesis effect from ethyl acetate fraction of senggani root, especially at sperm motility and sperm count. Decrease of sperm motility after treatment was compared with negative control group. The percentage of the sperm count decreased at 500; 250 mg/kg body weight dose from ethyl acetate fraction was 49,74 % and 48,21.This decreased probably were caused by seminiferous tubules necrotic.   ABSTRAK Pemberian ekstrak etanol akar senggani (Melastoma affine D. Don) telah terbukti dapat menyebabkan berkurangnya jumlah dan motilitas sperma tikus. Berdasarkan hasil penelitian tersebut dilakukan fraksinasi ekstrak etanol akar senggani dengan etil asetat kemudian fraksi itu di uji cobakan pada tikus dengan tujuan untuk eksplorasi bahan alam yang dapat dipakai sebagai bahan kontrasepsi pria. Penelitian ini menggunakan 32 ekor tikus jantan fertil galur Wistar, umur 2,5-4 bulan dengan bobot 145-350 g dan dibagi menjadi 5 kelompok. Empat kelompok merupakan kelompok perlakuan fraksi etil asetat akar senggani dengan dosis 500; 250; 125; dan 62,5 mg/kgBB. Kelompok V merupakan kelompok kontrol negatif dengan perlakuan CMC Na 0,5 %. Semua kelompok diberi perlakuan secara per oral selama 22  hari dengan frekuensi pemberian sekali sehari. Pada hari ke-23 tikus dikorbankan dan diamati motilitas, dan jumlah atau angka sperma. Dilakukan juga pengamatan histopatologi organ testis. Hasil penelitian menunjukkan ada pengaruh pemberian fraksi etil asetat akar senggani terhadap motilitas dan jumlah (angka) sperma. Motilitas sperma menjadi berkurang pada semua dosis perlakuan jika dibandingkan dengan kelompok kontrol. Penurunan motilitas sperma terbesar pada dosis 250 mg/kgBB. Jumlah sperma juga mengalami penurunan untuk semua dosis perlakuan. Persentase penurunan jumlah sperma terbesar pada dosis 500 ; 250 mg/kgBB sebesar 49,74 % , dan 48,21 %. Penurunan ini kemungkinan diakibatkan oleh rusaknya tubulus seminiferus testis yang mengalami nekrosis
Penetapan Kadar Alkaloid Ekstrak dari Etanolik Bunga Kembang Sepatu (Hibiscus rosa-sinensis L.) Murrukmihadi, Mimiek; Wahyuono, Subagus; Marchaban, .; Martono, Sudibyo
Jurnal Farmasi Indonesia Vol 6, No 3 (2013)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.643 KB) | DOI: 10.35617/jfi.v6i3.134

Abstract

Kembang sepatu flower (Hibiscus rosa-sinensis L.) was fractionally used as expectorant. Based on Bioassay Guided fractionation, an active fraction was separated, and the fraction was identified is Alkaloid was the major compound based on TLC analysis. Viscosity value measured by viscometer was used as a Bioassay model of expectorant activity in vitro and acetyl cysteine was used as positive control. Alkaloid content determination of the ethanolic extract was measured by TLC-Densitometric compared with standard curve of isolated alkaloid as the selected marker (Y=12,1360X+2901,4474). The alkaloid content in the ethanolic extract was determined as 2.35±0,67%.Keywords : alkaloid, ethanolic extract, Hibiscus rosa-sinensis L.
Co-Authors Abdul Karim Zulkarnain Abdul Rohman Aditya Krishar Karim Ag. Yuswanto Agung Endro Nugroho Agustinus Yuswanto, Agustinus Ahmad Hamim Sadewa Ambarwati Ambarwati Ari Sartinah, Ari Ariani Hatmanti Arnida ., Arnida AWM. Boersma, AWM. B.S. Ari Sudarmanto, B.S. Ari Bambang Hariono Citra Prasetyawati, Citra Dinar Sari Djoko Santosa Djoko Santoso Ekowati Chasanah Endang Dwi Wulansari Endang Lukitaningsih Erna Prawita Setyowati Farisya Nurhaeni Fauziati, Vera Galih G, Galih Galih, G. Gemini Alam Hadi, Etik Erna Wati Hari Susanti HERNAYANTI HERNAYANTI Hosea Jaya Edy Ibnu G Gandjar, Ibnu G Ibnu G. Gandjar, Ibnu G. Ibnu G. Ganjar, Ibnu G. Ika Puspita Sari Imono A. Donatus, Imono A. Imono Argo D, Imono Imono Argo Donatus, Imono Argo Indah Purwantini Isnindar Isnindar, Isnindar JAKA WIDADA K. Nooter, K. Kaneko, Takushi Kazutaka maeyama Kumiko Terada KV Rao, KV L. Hartanto Nugroho LANGKAH SEMBIRING Linus Yhani Chrystomo Lukman Hakim Mae S.H. Wahyuningnsih, Mae S.H. Mae S.H. Wahyuningsih Mae Sri Hartati W, Mae Sri Mae Sri Hartati Wahyuningsih, Mae Sri Hartati Mae Sri Hartati, Mae Sri Mahardika, Arfian Bela Marchaban ., Marchaban Marchaban Marchaban, Marchaban Marchaban, . Marchaban, . Mark T Hamann, Mark T Mark T Hamman Mark T. Hamann, Mark T. Mimiek Murrukmihadi Moch. Saiful Bachri Mohd., Datin Maryati mufrod mufrod Muhammad Nursid Murwantoko Murwantoko Mustofa . Nanang Fakhrudin, Nanang Nunung Yuniarti Nur Hidayati Nuringtyas, Tri R. Nurlaila Nurlaila Peni Susilowati Putri, Peni Susilowati Perdana Priya Haresmita, Perdana Priya Pratiwi, Sylvia Puji Astuti Purwantiningsih, Purwantiningsih PUSPITA LISDIYANTI R.L.M. Bolhuis, R.L.M. Rarastoeti Pratiwi Ratna Asmah Susidarti Retno Wahyuningrum Rijai, Hifdzur Rashif Ritmaleni Ritmaleni RR. Endang Lukitaningsih S.M. Widyastuti Sakti, Dika Sotya Samsul Hadi Sebastian Margino Sita Rina Widyarini, Sita Rina Sitarina Widyarini Soesanto Tjokrosonto Sofia Mubarika Sorta Basar Ida Simanjuntak Sri Sudewi, Sri Sri Wahdaningsih, Sri Sudarsono . Sudibyo Martono Sugeng Riyanto Sukarti Moeljopawiro Sutomo Sutomo suwaldi suwaldi Syamsudin . Tamhid, Hady Anshory Tanti Azizah S Tanti Azizah Sujono Tatang Irianti Tatsuo Takeya, Tatsuo Titik Nuryastuti, Titik Tri Lestari Tri R Nuringtyas, Tri R Triana Hertiani Tsutomu Nohno Vairappan, Charles S. Wahyono Wahyono Wahyuningsih, Mae Sri Hartanti Wayan Tunas Artama Widyastuti, S M Widyastuti, SM Widyastuti, SM Widyastuti, SM Yoga Pratama, Yoga Yuli Widiyastuti Yustina Sri Hartini, Yustina Sri Yuswanto Yuswanto Yuwantiningsih, Sri