Febri Walpajri
Jurusan Biologi, FMIPA, Universitas Riau

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EKSPLORASI DAN UJI DAYA HAMBAT BAKTERI ENDOFIT DARI TANAMAN BENALU SAWO (Helixanthera sp.), BENALU COKLAT (Scurulla sp.) DAN BENALU KOPI (Helixanthera sp.) TERHADAP Escherichia coli Walpajri, Febri; Roza, Rodesia Mustika; ', Fitmawati
Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam Vol 1, No 2 (2014): Wisuda Oktober 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Matematika dan Ilmu Pengetahuan Alam

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Abstract

Endophytic bacteria are microscopic microorganisms that live in the tissues of plants such as leaves, roots, fruits and stems. The parasite plants on sapodilla (Helixanthera sp.), cocoa (Scurulla sp.) and coffee (Helixanthera sp.) are medicinal plants. Theseplants have endophytic bacteria that have antibacterial compounds as antibiotics that inhibit the growth of Escherichia coli. The purpose of this study was to get endophytic bacteria isolates from parasite plants on sapodilla, cocoa and coffee, and determine the antibacterial activity against E. coli. Sampling was conducted in Kampar regency and Pekanbaru city. The parasite plants were collected from the field and their endophytic bacteria  were selected using surface sterilization method and purified before their  activity against E. coli being tested. The selected bacteria were then characterized. The results  obtained  34  endophytic  bacterial  isolates  that  had  activity  against  E.  coli. Endophytic bacteria isolates which had the highest activity against E. coli isolates were  Bbs4 from sapodilla parasites with 12,1 mm inhibition zone diameter.
MIKROBA ENDOFIT “SI PEMBUNUH” Escherichia coli Walpajri, Febri; Rohyani, Rohyani; Umayah, Sari
Program Kreativitas Mahasiswa - Penelitian PKM-P 2014
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

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Abstract

Endophytic microbes are microscopic microorganisms (bacteria and fungi) that live in the tissues of plants, leaves, roots, fruits and stems. The parasite plants (Helixanthera sp.) on sapodilla (Manilkara zapota), cocoa (Theobroma cacao) and coffee (Coffea arabica) are medicinal plants. These plants have endophytic microbes that have antibacterial compounds as antibiotics. The purpose of this study was to get endophytic microbial isolates from parasite plants (Helixanthera sp.) on sapodilla, cocoa and coffee, and determine the antibacterial activity against Escherichia coli. Sampling was conducted in Kampar regency and Pekanbaru city. The parasite plants were collected from the field and their endophytic microbes were isolated using surface sterilization method and purified before being tested the activity against E. coli. The selected microbes were then characterized. The results obtained 30 endophytic bacterial isolates and 10 endophytic fungi that had activity against E. coli. Endophytic bacteria isolate which had the highest activity against E. coli  was Bbs4 isolate from sapodilla parasites with 12,1 mm  inhibition zone diameter and endophytic fungi isolate which had the highest activity against E. coli was Jbc2 isolate (genus Penicillium)  from cocoa parasites with 12,1 mm inhibition zone diameter. Keywords:  antibacterial, endophytic microbes, Escherichia coli, parasite plant