Articles

Found 10 Documents
Search

IMOBILISASI ENZIM LIPASE PADA PADATAN PENDUKUNG ZEOLIT ALAM Wardoyo, Fandhi Adi; Kartika, Aprilia Indra
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.878 KB)

Abstract

Lipase is one kind of enzyme that widely use. Lipase has a great catalytic power, but easily influenced by environments and difficult to separate at the ends of the reaction, so it can not be re-used. Based on that, one solution to overcome that problem is through the enzyme immobilization. Pancreaticlipase was immobilized by absorption method in zeolit as solid support. The aim of this reseacrh is to determine the optimum pH of lipase solubility. The activity of immobilized lipase was tested through the hydrolysis reaction of palm oil. The optimum pH for enzyme dissolution was obtained at pH 6 .Pancreatic lipase and immobilized lipase on zeolite can produce free fatty acid as much as17,37% and 7,35% respectively.Keywords: lipase, immobilization, zeolit, absorption
WAKTU PAPARAN GAS OZON TERHADAP PERTUMBUHAN BAKTERI ESCHERICHIA COLI Ma?ruf, Amal; Dewi, Sri Sinto; Wardoyo, Fandhi Adi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.658 KB)

Abstract

Ozone is one of the gases that can be used as a disinfectant to inhibit the growth of Escherichia coli bacteria. Time of exposure to ozone gas is a way used to disinfectEscherichia coli bacteria. The purpose of this study was to observe the number ofEscherichia coli bacteria exposed to ozone gas with exposure time of 5, 10, 15, 20, 25and 30 minutes and ozone gas control. The study sample was Escherichia colibacteria exposed to ozone gas. The results of the number of colonies of Escherichiacoli on CCA media after exposure to 5, 10, 15 minutes of ozone gas were mean 2.5 x106, 1.25 x 106, 0.5 x 106 CFU / ml, and 20, 25, 30 minutes there is no bacterialgrowth. Control or no exposure to ozone gas mean is 9 x106 CFU / ml. The time ofexposure to ozone gas is most effective in killing Escherichia coli bacteria ie at 20minutes.Keywords: ozone gas, Escherichia coli, Exposure time.
PENGARUH DERAJAD DEASETILASI KITOSAN DAN PH PELARUTAN ENZIM TERHADAP KEMAMPUAN IMOBILISASI LIPASE PADA KITOSAN SERBUK Wardoyo, Fandhi Adi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2014: PROSIDING SEMINAR NASIONAL HASIL - HASIL PENELITIAN & PENGABDIAN
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (950.124 KB)

Abstract

Immobilization of lipase on chitosan powder was performed. The aim of this research is todetermine the optimum conditions for the immobilization process. Chitosan was prepared fromcrab shell through continous deacetylation, and crosslinked with glutaraldehyde. Immobilizationof lipase on chitosan powder was studied under the optimum conditions e.i. pH optimum oflipase solubility and deacetylation degree of chitosan. Immobilized lipase on chitosan powderwas tested for the activity through the hydrolysis reaction of palm oil. Continous deacetylationproduced chitosans A, B and C with deacetylation degrees of 81.34% , 90.23%, and 92.80%,respectively. The optimum pH for enzyme dissolution was obtained at pH 6. Optimumefficiency of immobilization on chitosans A, B, and C, were 47.67; 40.74 and 38.76%,respectively. Specific activity of free lipase, immobilized lipase on chitosan A, immobilizedlipase on chitosan B and immobilized lipase on chitosan C were 23.94; 0.0435; 0.0178; and0.0175 U/g, respectively.Keywords: lipase, immobilization, chitosan, cross linking
Peningkatan Stabilitas Penggunaan Berulangd an Stabilitas Termal Enzim Lipase melalui Imobilisasi pada Zeolit Alam Wardoyo, Fandhi Adi; Kartika, Aprilia Indra
Jurnal Labora Medika Vol 2, No 1 (2018): Jurnal Labora Medika
Publisher : Jurnal Labora Medika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Thermal stability and lifetime stability for immobilization lipase on zeolit by absorption technique has been carried out. Immobilization of lipase on zeolit was performed via physical adsorption. This reseacrh aimed to determine the effectiveness of pancreatic lipase immobilization in term of thermal and lifetime stability of the hydrolysis activity. The result of this research proved that pancreatic lipase was able to immobilized to zeolit by using the physical adsorption. Immobilized lipase shows better lifetime stability than the free lipase. Immobilized enzyme catalyzed reaction has a lower decreases free fatty acid than that of the free enzyme. Lower decline of value suggests the enzyme is more stable for repeated use. The immobilized lipase was able to maintain its activity after multiple-uses up to four reaction cycles. Similar trend was also observed for thermal stability study. Immobilized lipase shows a higher thermal stability that that shown by free lipase. Immobilized lipase on zeolit was also able to maintain its activity at 40oC.
PENURUNAN BILANGAN PEROKSIDA PADA MINYAK JELANTAH MENGGUNAKAN SERBUK DAUN PEPAYA Wardoyo, Fandhi Adi
Jurnal Pangan dan Gizi Vol 8, No 2 (2018): Kajian Pangan dan Gizi
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.421 KB) | DOI: 10.26714/jpg.8.2.2018.82-90

Abstract

Research on the reduction of peroxide numbers in wasted cooking oil has been done. The aim of this research is to determine the reduction of peroxide numbers in wasted cooking oil after soaking with papaya leaf powder (Carica papaya) concentration 5, 10, 15 and 20 %b/v, then it has been soaked for one to five days. The wasted cooking oil has initial peroxide numbers sebesar 74,29 mg O2/100. The result showed that after wasted cooking oil has been soaked for five days with papaya leaf powder concentration 10%b/v can reduced peroxide number up to 52,16%, and the peroxide number decreased to 35,54 mg O2/100.
PROFIL PROTEIN TIGA JENIS DAGING YANG DILUMURI SERBUK DAUN PEPAYA BERBASIS SDS-PAGE Kustia, Nevi; Darmawati, Sri; Wardoyo, Fandhi Adi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.495 KB)

Abstract

Papaya leaves contain protease enzymes (protein decomposers), namely papain and kimopapain. Both of these enzymes have the ability to break bonds in protein molecules so that proteins break down into polypeptides and dipeptides. If working on the meat can be decomposed so that the meat becomes tender. The purpose of this study was to determine protein profiles in three types of meat before and after smeared with papaya leaf powder with 60 minutes of immersion. The protein profile of three meat types was analyzed using the SDS-PAGE method. The design of this research is descriptive research with the object of research are goat meat, buffalo and cow covered with papaya leaf powder. The result of this research shows that there are meat of buffalo, goat and cow there are many major and minor ribbon, while in buffalo meat, goat and cow which has been smeared with papaya powder there are many minor protein bands. These results indicate that papain enzyme contained in papaya leaves are powdered to break peptide bonds in meat proteins to proteins in the form of minor bands (micromolecules) and show that the higher concentration of papaya leaf powder the more denatured the protein that is on 20% concentration has only 3 protein bands.Keywords: Meat, Papaya Leaf, Protein Profile, SDS-PAGE
PENGARUH PASANG DAN SURUT AIR LAUT TERHADAP KADAR ZAT ORGANIK DAN OKSIGEN TERLARUT PADA AIR SUMUR DI KELURAHAN TANJUNG MAS SEMARANG Puspitasari, Anggraeni; Mukaromah, Ana Hidayati; Wardoyo, Fandhi Adi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.685 KB)

Abstract

Telah dilakukan pemeriksaan kadar zat organik dan kadar oksigen terlarut pada air sumur di kelurahan Tanjung Mas Semarang  pada saat pasang dan surut air laut.  Tujuan dari penelitian untuk mengetahui pengaruh pasang dan surut air laut terhadap kadar zat organik dan oksigen terlarut pada air sumur di kelurahan Tanjung Mas Semarang. Sampel penelitian menggunakan air sumur yang diambil dari lima sumur warna yang terdekat dengan air laut. Penetapan kadar zat organik ditetapkan dengan metode permanganat dan kadar oksigen terlarut ditetapkan dengan metode iodometri. Dari hasil penelitian  diperoleh kadar zat organik pasang berturut-turut 33,26 mg/L; 32,38 mg/L; 26,25 mg/L; 20,17 mg/L dan 18,95 mg/L dan surut 44,99 mg/L; 88,84 mg/L; 33,35 mg/L; 25,63 mg/L dan  22,86 mg/L. Kadar oksigen terlarut pasang dari 5 sumur berturut-turut 4,90 mg O2/L; 5,17 mg O2/L; 5,95 mg O2/L, 6,95 mg O/L dan 7,09 mg O2/L dan surut 3,36 mg O2/L; 4,31 mg O2/L; 4,95 mg O2/L; 5,44 mg O2/L dan 6,41 mgO/L. Ada pengaruh pasang dan surut air laut terhadap kadar zat organik dan oksigen terlarut pada air sumur di kelurahanTanjung Mas Semarang. Kata Kunci : Kelurahan Tanjung Mas, Kadar Zat Organik, Kadar Oksigen Terlarut, Air Laut Pasang, Air Laut Surut.
Penurunan Kadar Timbal dalam Air Menggunakan Bentonit Teraktivasi Wardoyo, Fandhi Adi
Jurnal Biomedika Vol 9 No 2 (2016): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (512.421 KB) | DOI: 10.31001/biomedika.v9i2.216

Abstract

Timbal merupakan salah satu bahan pencemar yang banyak terdapat di dalam perairan. Timbal di perairan yang masuk ke dalam tubuh dapat mempengaruhi sistem syaraf, hormon dan juga jantung. Untuk mengurangi pencemaran timbal di perairan, maka diperlukan suatu adsorben yang mampu menyerap timbal. Salah satu adsorben yang banyak digunakan adalah bentonit. Tujuan dari penelitian ini adalah untuk mengetahui penurunan kadar timbal dalam perairan menggunakan bentonit yang telah diaktivasi. Bentonit yang digunakan merupakan bentonit alam yang diayak lolos 170 mesh. Bentonit selanjutnya diaktivasi menggunakan H3 PO4 KCl dan NH4 OH dengan konsentrasi 0,05M. Waktu kontak dilakukan selama 1 jam. Dari hasil penelitian didapatkan bahwa kondisi optimum didapat dari bentonit teraktivasi NH4 OH dengan konsentrasi teradsorpsi rata-rata sebesar 86,36 ppm.
UJI STABILITAS ENZIM LIPASE TERIMOBILISASI PADA KITOSAN SERBUK MELALUI TEKNIK TAUT SILANG Wardoyo, Fandhi Adi; Raharjo, Tri Joko; Swasono, Respati Tri
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.718 KB)

Abstract

Lipase has a great catalytic power, but easily influenced by environments and difficult to separate at the ends of the reaction, so it can not be re-used. Based on that, one solution to overcome that problem is through the enzyme immobilization. Immobilization of lipase on chitosan powder was performed via cross linking technique. Chitosan was prepared from crab shell with deacetylation degree 82,34% and crosslinked with glutaraldehide. The aims of this research is to determine the thermal stability and multiple-use stability through the hydrolysis reaction of palm oil. Immobilized lipase on chitosan powder was able to maintain its activity at 50 oC, and still can produce free fatty acid as much as 48,55%. The immobilized lipase was also able to maintain its activity after multiple-uses up to five reaction cycles, and still can produce free fatty acid as much as 40,82%.Keywords: lipase, immobilization, chitosan, cross linking
Peningkatan Stabilitas Penggunaan Berulangd an Stabilitas Termal Enzim Lipase melalui Imobilisasi pada Zeolit Alam Wardoyo, Fandhi Adi; Kartika, Aprilia Indra
Jurnal Labora Medika Vol 2, No 1 (2018): Jurnal Labora Medika
Publisher : Jurnal Labora Medika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Thermal stability and lifetime stability for immobilization lipase on zeolit by absorption technique has been carried out. Immobilization of lipase on zeolit was performed via physical adsorption. This reseacrh aimed to determine the effectiveness of pancreatic lipase immobilization in term of thermal and lifetime stability of the hydrolysis activity. The result of this research proved that pancreatic lipase was able to immobilized to zeolit by using the physical adsorption. Immobilized lipase shows better lifetime stability than the free lipase. Immobilized enzyme catalyzed reaction has a lower decreases free fatty acid than that of the free enzyme. Lower decline of value suggests the enzyme is more stable for repeated use. The immobilized lipase was able to maintain its activity after multiple-uses up to four reaction cycles. Similar trend was also observed for thermal stability study. Immobilized lipase shows a higher thermal stability that that shown by free lipase. Immobilized lipase on zeolit was also able to maintain its activity at 40oC.