I WAYAN TEGUH WIBAWAN
Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan Institut Pertanian Bogor

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RESISTANSI ESCHERICHIA COLI TERHADAP KOLISTIN DAN DETEKSI GEN MOBILIZED COLISTIN RESISTANCE-1 PADA AYAM PEDAGING AKIBAT PEMBERIAN KOLISTIN SULFAT Palupi, Maria Fatima; Maheshwari, Hera; Darusman, Huda Salahuddin; Sudarnika, Etih; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2018.19.2.196

Abstract

Colistin sulphate is the ultimate antimicrobial choice for the treatment of multidrug resistance gram negative bacteria infections with in human. The purposes of this study were to detect the presence of colistin resistant E. coli and mcr-1 gene in broiler and to transfer the mcr-1 gene to Salmonella enteritidis ATCC 13076. A total of 54 one day old broilers were divided into three groups that consists of 18 chicks broiler per group and raised up to 40 days old. The first group was used as control. The first treatment group was given colistin sulphate 5 ìg/g feed for 40 days and broilers in second treatment group was given 80.000 IU/kg body weight for first three days. Swab cloaca samples were taken every 10 days from each broiler. At age 40 days all chickens were slaughtered and meat samples were collected. Samples of cloacal swabs, fresh and cooked meat were examined for the presence of colistin resistant E. coli and mcr-1 gene. Susceptibility to colistin sulfate was conducted by agar dilution method, and detection of mcr-1 gene was conducted using polymerase chain reaction. The results showed that no colistin resistant E. coli was detected in the control group. Colistin resistant E. coli (27.78%) and mcr-1 gene (20.00%) were detected in animals in the first treatment group, respectively. Whilst 11.11% colistin resistant E. coli and 5.56% were carriying mcr-1 gene in the second treatment group. Colistin resistant E. coli were found 5.56% from raw meat samples and 3.70% had mcr-1 gene. Transfer of mcr-1 gene from colistin resistant E. coli to Salmonella enteritidis ATCC 13076 was success. These results showed the necessity of limitation usage of colistin sulphate in food animal. 
PROFIL IMUNOGLOBULIN-G SERUM KAMBING PERANAKAN ETAWAH BUNTING YANG DIBERI IMBUHAN PAKAN MINERAL SENG (SERUM IMUNOGLOBULIN-G LEVEL ON PREGNANT ETTAWAH CROSSBRED WERE GIVEN ZINC MINERAL) Widhyari, Sus Derthi; Esfandiari, Anita; Sutama, I Ketut; Widodo, Setyo; Wibawan, I Wayan Teguh; Ramdhany, Rizal Rahadian
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.1.24

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The objective of this study was to find out the effect of zinc supplementation on the profile of immunoglobulin-G (IgG) in the pregnant etawah-cross ewes. Fifteen etawah-cross ewes (3-6 years old and 30-50 kg body weight) were used in this experiment and they were divided into three groups each of which consisted of 5 ewes. The first group (Zn40) received 40 ppm Zn as control, the second group (Zn60) received 60 ppm Zn, and the third group (Zn80) received 80 ppm Zn. Blood samples were collected from jugular vein every two weeks, started at twelve weeks of pregnancy up to eight weeks post partum for immunoglobulin-G analysis. IgG level were analyzed by competitive Enzyme Linked Immunosorbent Assay (ELISA) technique. Results showed that there were no significant difference of immunoglobulin-G level (P>0,05) among the three treatment groups ( Zn40, Zn60, and Zn80). However, IgG level of Zn40 and Zn80 groups tended to decrease during the last staget of pregnancy until two weeks post partum. In conclusion, supplementation of 60 ppm Zn showed higher IgG level than Zn 80 ppm. ABSTRAK Penelitian ini bertujuan untuk mengetahui efek suplementasi seng (Zn) terhadap imunoglobulin-G (IgG) pada kambing peranakan etawah (PE) bunting. Penelitian ini menggunakan 15 ekor kambing PE umur sekitar tiga sampai enam tahun, bobot badan sekitar 30-50 kg dibagi ke dalam tiga kelompok, masing-masing terdiri dari lima ekor. Kelompok Zn40 (kontrol) diberi pakan mengandung mineral Zn 40 ppm, kelompok Zn60 mengandung mineral Zn 60 ppm, dan kelompok Zn80 mengandung mineral Zn 80 ppm. Sampel darah diambil melalui vena jugularis untuk menganalisis konsentrasi IgG. Pengambilan sampel darah dilakukan setiap dua minggu mulai umur kebuntingan 12 minggu sampai delapan minggu setelah melahirkan. Hasil penelitian menunjukkan bahwa konsentrasi IgG serum tidak menunjukkan perbedaan secara nyata antar kelompok (P>0,05) antara kelompok Zn 0, Zn 40 dan Zn 80 ppm. Kelompok Zn 40 ppm dan 80 ppm cendrung memperlihatkan konsentrasi IgG mengalami penurunan pada akhir kebuntingan sampai dua minggu setelah melahirkan. Pada kelompok Zn 60 ppm memperlihatkan konsentrasi IgG lebih tinggi dibanding kelompok Zn 80 ppm.
KONSENTRASI PROTEIN TOTAL, ALBUMIN, DAN GLOBULIN ANAK KAMBING PERANAKAN ETAWAH SETELAH PEMBERIAN BERBAGAI SEDIAAN KOLOSTRUM* (TOTAL PROTEIN, ALBUMIN, AND GLOBULIN CONCENTRATIONS ON ETTAWAH CROSSBREED NEONATES FOLLOWING THE ADMINISTRATION OF VARIOUS FORM O Esfandiari, Anita; Widhyari, Sus Derthi; Widodo, Setyo; Wibawan, I Wayan Teguh; Sajuthi, Dondin; Sutama, I Ketut
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This experiment was conducted to study the profile of total protein, albumin, and globulin concentrationson Ettawah crossbreed neonates after consuming various colostrums. Twenty four healthy neonatal kidswere used in this study. The neonates were divided into four groups. Each group received fresh maternal(goat) colostrum, frozen-thawed bovine colostrum, bovine spray dried colostrum, and bovine powdercommercial colostrum, respectively. Colostrums were given at 10% of body weight directly after birth andfollowed by the same amount every 12 hours, for three days. The blood was taken from jugular vein at 0, 12,24, 48, 72, and 168 hours after birth to determine total protein, albumin, and globulin concentrations.Results of this study indicated that the serum total protein and globulin concentration increased andreached the peak at 24 hours after birth. Compared to the concentration at birth, the increase of totalprotein concentration were 62.77%, 59.26%, 48.05%, and 66.67% in fresh maternal (goat), frozen-thawedbovine, bovine spray dried, and commercial bovine colostrum, respectively. Serum globulin concentrationincreased 4.9, 4.4, 4.8, and 14.6 times in fresh matermnal goat, frozen-thawed bovine, spray dried, andcommercial bovine colostrums respectively, compared to the concentration at birth. In conclusion, theconsumption of various colostrums i.e. fresh maternal goat colostrums, bovine colostrums (frozen-thawed,spray dried and commercial colostrums) would increase the concentration of blood total protein and globulin,which both reached the highest concentration at 24 h after birth.
KINERJA KESEHATAN SAPI NEONATUS YANG DIBERI KOLOSTRUM DARI INDUK SAPI YANG DIVAKSIN ESCHERICHIA COLI Wulansari, Retno; Esfandiari, Anita; Wibawan, I Wayan Teguh; Murtini, Sri
Acta VETERINARIA Indonesiana Vol. 4 No. 1 (2016): Januari 2016
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.496 KB) | DOI: 10.29244/avi.4.1.19-26

Abstract

Penelitian ini dilakukan untuk mempelajari status kesehatan anak sapi baru lahir yang diberi kolostrum berasal dari induk yang telah divaksinasi dangan vaksin E. coli in-aktif. Sebelas ekor anak sapi baru lahir digunakan dalam penelitian ini dibagi menjadi dua kelompok yaitu kelompok kolostrum (diberi kolostrum sapi) dan non-kolostrum (diberi susu sapi). Kolostrum atau susu sapi diberikan sebanyak 10% berat badan secara langsung setelah dilahirkan dan selanjutnya tiap 12 jam selama 3 hari. Uji tantang dilakukan dengan pemberian E. Coli K99 hidup peroral saat anak sapi berumur 14 jam. Pemeriksaan status kesehatan termasuk suhu tubuh, frekuensi pulsus dan napas serta kualitas defikasi pada 0,12, 24, 48, 72 dan 168 jam setelah uji tantang. Hasil penelitian ini memperlihatkan bahwa diarrhea timbul pada 12 sampai 26 jam setelah uji tantang pada semua anak sapi kelompok non-kolostrun dan beberapa anak sapi kelompok kolostrum. Kelompok non-kolostrum memperlihatkan tanda klinis dengan diarrhe parah sebagai diarrhe profus yang berwarna pucat kekuningan. Bahkan 1 ekor mati pada 3 hari setelah uji tantang. Sebaliknya pada kelompok kolostrum hanya memperlihatkan diarrhe sedang pada 5 dari 8 ekor. Kesimpulan dalam penelitian ini adalah konsumsi kolostrum yang berasal dari induk yang telah divaksinasi dengan E. Coli memperlihatkan sifat protektif terhadap infeksi E. coli K99.Kata kunci: Escherichia coli, kolostrum, anak sapi baru lahir. (The Health Performance of Neonatal Calves Received Colostrum from Cows Vaccinated by Escherichia coli)This experiment was conducted to study the health status of neonatal calves received colostrum from cows vaccinated by in-active E. coli. Eleven healthy newborn calves were used in this experiment and divided into two groups, i.e colostrum (received bovine colostrum) and non-colostrum group (received bovine milk). Colostrum or milk were given to the calves at 10% of body weight directly after birth and followed every 12 h, for three days. Challenges were done orally to all newborn calves when they were 14 hours of ages, used live E. coli K-99. Examination of health status included body temperature, pulses and respiration rates, and defecations quality at 0, 12, 24, 48, 72 and 168 hours after challenges. Results of the experiment showed that diarrhea appeared at 12-26 hours after challenges in all calves of non-colostrum group and part of calves in colostrum group. The non-colostrum group showed a severe clinical signs of diarrhea as watery profuse diarrhea with pale yellowish color. One calf even death at three days after challenges. In contrast, the colostrum group showed only a mild diarrhea in 5 out of 8 calves. In conclusion, the consumption of colostrum originated from cows vaccinated by E. coli showed protective properties against E. coli K-99 infection.Keywords: Escherichia coli, colostrum, neonatal calves
KINETIKA IMMUNOGLOBULIN KUNING TELUR ANTIPARVOVIRUS ANJING PADA ANJING (KINETICS OF ANTICANINE PARVOVIRUS YOLK IMMUNOGLOBULIN IN DOGS) Suartini, I Gusti Ayu Agung; Sendow, Indrawati; Agustini, Ni Luh Putu; Suprayogi, Agik; Wibawan, I Wayan Teguh; Mahardika, I Gusti Ngurah Kade
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Kinetic study on Anti CPV IgY has been performed on six dogs aged 5-10 months. The IgY was injectedintravenously at dose of 21.4mg /10kg body weight. IgY levels in the blood were determined by ELISA. Aresearch was conducted to find out the kinetics of Anti CPV IgY in dogs blood. The kinetics of IgY wascalculated by using regression analysis to determine the association on the levels of IgY in serum againsttime at injection. The results showed that kinetic parameters were calculated based on first order kinetics.The constant elimination rate of IgY was at the range between 0.007 to 0.015 / h. IgY concentration in thedogs blood was from 0.746 to 0.992 mg / mL. The half-life of IgY was from 1.65 to 4.01 / d. Volumedistribution of IgY was between 21.47 to 28,55 / mL. Total IgY in the dog bodies (AUC) was from 42,60 to142,00 mg / mL.h. The duration of the IgY in the dog?s body was 3.08 to 8.51 days. Clearance time of IgY was0.15 to 0.50 mL / h. In conclusion the kinetics of anti CPV IgY in dog?s body follow one compartment andfirst order model, which are only distributed in the blood with the half-life at 2.5 days, and IgY has lesspossibility to accumulate in the body compared to the IgG.
PEMANFAATAN TELUR AYAM SEBAGAI PABRIK BIOLOGIS (KAJIAN PUSTAKA) WIBAWAN, I WAYAN TEGUH
Majalah Ilmiah Peternakan Vol 11 No 1 (2008)
Publisher : Fakultas Peternakan Universitas Udayana

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Abstract

ABSTRAK Penelitian tentang pemanfaatan telur sebagai pabrik biologis dilakukan sangat komprehensif di beberapa institusi perguruan tinggi di Inonesia, yakni Fakultas Kedokteran Hewan (FKH) Institut Pertanian Bogor (IPB), FKH Universitas Gadjah Mada (UGM), FKH Universitas Udayana (UNUD), FKH Universitas Syahkuala (Unsyiah), Fakultas Kedokteran Gigi Universitas Indonesia. Jejaring penelitian dibangun dengan baik dan telah menghasilkan beberapa produk, antara lain: telur berkhasiat flu burung H5N1, telur Anti Tetanus Serum, Telur Anti Diare, Telur anti Plaque dan Telur Anti White Spot Syndrome Virus pada udang. Secara ilmiah khasiat IgY spesifik dalam kuning telur sebagai senyawa therapeutic telah diuji dan tinggal memerlukan sentuhan akhir tersendiri untuk dapat disajikan sebagai produk komersial. Peran industri yang relevan sangat dibutuhkan dalam mewujudkan hal ini. THE USE OF EGG AS A BIOLOGICAL PRODUCER FOR SPESIFIC ANTIBODIES ABSTRACTS The use of egg as a biological producer for specific antibodies were done intensively among well known universities in Indonesia, such as Faculty of Veterinary Medicine (FVM) of FVM of IPB, FVM of UGM, FVM of UDAYANA and FVM of UNSYIAH as well as Faculty of Dental Medicine Indonesia University. The collaboration and research networking among those institutions run as expected and succeed to produce some important products such as eggs containing IgY anti avian flu as well as anti tetanus, anti diarrhea, anti plaques and eggs anti WSSV in shrimp. The potency of specific IgY as immunotherapeutic substances had been studied and need the commercial touch from the respective industries until this products in market available.
CROSS REACTION OF SERUM IN SALMONELLA ENTERITIDIS- VACCINATED CHICKEN TO SOME SALMONELLA ENTERICA SEROTYPES (REAKSI SILANG SERUM AYAM YANG DIVAKSIN DENGAN SALMONELLA ENTERITIDIS TERHADAP BEBERAPA SEROTIPE SALMONELLA ENTERICA) Arnafia, Wyanda; Ningrum, Siti Gusti; Puspita, Erfiandini Eka; Lukman, Denny Widaya; Pasaribu, Fachriyan Hasmi; Wibawan, I Wayan Teguh
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Salmonella spp. has been recognized as the major cause of food-borne illness in humans worldwidecausing remain relevant to public health. Poultry vaccination is one promising strategy to mitigateSalmonella infection in poultry and, in turn, in humans as well. The objective of this study was to assessthe potential of cross-reaction of serum in Salmonella enteritidis-vaccinated chicken to some serotype ofSalmonella enterica. Four female, Isa Brown layer chickens (20 weeks old), were vaccinated with S. enteritidisstrain Sm24/Rif12/Ssq (intra vena) to induced the production of specific antibodies in serum. Crossreactionof serum in S. enteritidis-vaccinated chicken were assess with agar gel immunodiffusion test(AGID) with S. enteritidis, S. pullorum, S. typhimurium, S. typhi, and Escherichia coli antigens. Serumcould react with S. enteritidis and all types of S enterica used in this study (S. pullorum, S. typhimurium,S. typhi), but could not react with E. coli. The potential of cross-reaction of serum in S. enteritidis-vaccinatedchicken to some serotypes of S. enterica may play a role in reducing the infection caused by that serotype.
VAKSIN POLIVALEN UNTUK MENCEGAH PENYAKIT FLU BURUNG (POLIVALEN VACCINE TO PREVENT BIRD FLU DISEASES) Suartha, I Nyoman; Wirata, I Wayan; Putra, I Gusti Ngurah Narendra; Dewi, Ni Made Ritha Krisna; Anthara, I Made Suma; Wibawan, I Wayan Teguh; Mahardika, I Gusti Ngurah Kade
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was carried out to determine the use of bird flu polyvalent vaccines containing two or threeor more virus isolates representating of circulating viruses in the region. Three seed isolates of avianinfluenza H5N1 virus were used in this experiment. The isolates were Chicken/Denpasar/Unud-01/2004,Chicken/Klungkung/Unud-12/2006, and Chicken/Jembrana/Unud-17/2006. The seeds were inactivatedusing 0.01% formaldehide than mixed (AI3G) alumunium hidroxide adjuvant and then injectedintramuscularly to Isa Brown layer chicken at 3 weeks of age and repeated at the age of 5 weeks. The doseof each seed virus was 27 HA units. Sera were collected at one and two weeks after the second vaccination.The result showed that the arithmetic meant titer (AMT) of sera that tested with homologous isolate washigher than the test using a heterologous isolates, in the standard haemaglutination inhibition (HI) assay.The mixed AI3G vaccine produced a uniform AMT against the constituent isolates, while vaccines withindividual isolate yielded a lower and more variation in AMT. Further experiments using a commercialhomologous H5N1 and heterologous H5N2 commercial vaccines has resulted AMT that 1-4 log lower thanAI3G vaccine. It is concluded that polyvalent vaccine with field seed isolates is recommended to be appliedin the poultry farm in Indonesia.
ISOLASI DAN KARAKTERISASI HEMAGLUTININ STAPHYLOCOCCUS AUREUS PENYEBAB MASTITIS SUBKLINIS PADA SAPI PERAH Abrar, Mahdi; Wibawan, I Wayan Teguh; Priosoeryanto, Bambang Pontjo; Soedarwanto, Mirnawati; Pasaribu, Fachriyan Hasymi
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i1.347

Abstract

Dalam penelitian ini isolasi dan karakterisasi hemaglutinin Staphylococcus aureus dilakukan dengan teknik afinitas kromatograf Karakterisasi hemaglutinin yang dihasilkan dilajutkan dengan teknik elektroforesis menggunakan metode sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) dan dilanjutkan untuk melihat pengaruh suhu dan enzim terhadap aktivitas hemaglutinin. Hasil penelitia menunjukkan bahwa komponen hemaglutinin Staphylococcus aureus yang telah diisolasi memiliki berat molekul 46 kDa. Aktivit Staphylococcus aureus dalam meghemaglutinasi hilang pada pemanasan 60° C dan pengaruh enzim proteolitik. Hasil ini mengindikasika bahwa hemaglutinin Staphylococcus aureus adalah protein.
EFEKTIVITAS LARUTAN DESINFEKTAN DALAM MENGINAKTIVASI VIRUS AVIAN INFLUENZA PADA BULU UNGGAS Luwito, Bagus Nanang; Wibawan, I Wayan Teguh; Soejoedono, Retno Damayanti
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8406.954 KB) | DOI: 10.22146/jsv.26934

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Avian Influenza (AI) virus  is pathogenic agent that can spread from one area to another area through the transportation of infected animals or their products such as feathers. This research was aimed to inactivated AI virus with 775 ppm sodium hypochlorite and 0.1% chloroxylenol to examine the difference of treatment by time (day) for inactivation AI virus on the feathers. AI virus isolate A/chicken/sidrap/ 07160336?2/2016 used in this research was obtained from Balai Besar Veteriner Maros. The treatment of disinfectants were performed on the first day (the day of disinfectan solutions were prepared), the third day and the seventh day by soaked the feathers in disinfectant solution for 10 minutes. The effectiveness of disinfectans were evaluated by inactivation index. The result show that the average of inactivation index of 775 ppm of sodium hypochlorit was 4.17 for the first day, 5.17 for the third day, and 4.20 for the seventh day, while the average of inactivation index of 0.1% chloroxylenol was 5.50 for the first day, 6.43 for the third day, and 5.77 for the seventh day. Our result indicated that the sodium hypochlorit and chloroxylenol were effective for inactivation of AI virus. The 0.1% of chloroxylenol was more effective for inactivation AI virus than 775 ppm of sodium hypochlorit, whilst the most effective duration for the treatment is the three days.
Co-Authors Agik Suprayogi Agnesia Endang Tri Hastuti Wahyuni AGUSTIN INDRAWATI Ahmad Sulaeman Anak Agung Sagung Kendran Andrijanto H. Angi Anita Esfandiari Asep Gunawan, Asep BAMBANG PONTJO PRIOSOERYANTO Budi Setiawan Cece Sumantri Christoph Lammler Citra Noviana Darusman, Huda Salahuddin Darusman, Huda Shalahudin Denny Widaya Lukman DEWI APRI ASTUTI Diyantoro Diyantoro, Diyantoro DONDIN SAJUTHI Drajat Martianto Dyah Ayu Hewajuli Eko Sugeng Pribadi Endhie D. Setiawan, Endhie D. Etih Sudarnika Fachriyan H Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasymi Pasaribu Fachriyan Pasaribu, Fachriyan Feri Kusnandar Hadri Latif Harlystiarini, Harlystiarini HERA MAHESHWARI I Gusti Ayu Agung Suartini I Gusti Ngurah Kade Mahardika I Gusti Ngurah Kade Mahardika I Gusti Ngurah Narendra Putra I Ketut Sutama I Made Suma Anthara I Nyoman Suartha I Wayan Wirata Indrawati Sendow Iwan Harjono Utama Kallau, Novalino Harold Geoffrey Kallau, Novallino Kamil Riski Sidik, Kamil Riski Kereh, Veybe Gresje Kurniawati, Anindya Luwito, Bagus Nanang Maggy Thenawidjaja Suhartono Mahdi Abrar Mangaraja Pidoli Tampubolon MARDHIATI, RETNO Maria Fatima Palupi Mayasari, Ni Luh Putu Ika Michael Haryadi Wibowo Mirnawati Soedarwanto MIRNAWATI SUDARWANTO Mohammad Ashraf, Mohammad Muhsinin, Muhammad Muladno . Nahrowi Nahrowi Ni Luh Putu Agustini, Ni Luh Putu NI LUH PUTU INDI DHARMAYANTI Ni Made Ritha Krisna Dewi Niken Ulupi Nurbani Kalsum Puspita, Erfiandini Eka R Susanti Rachmawati, Faidah Rachmawati Ramdhany, Rizal Rahadian Retno Damajanti Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Wulansari Rita Mutia Setyo Widodo Siti Gusti Ningrum, Siti Gusti Siti Madanijah Sri Anna Marliyati Sri Murtini Sudarwanto, Mirnawati Baharudin Sus Derthi Widhyari Sylvia Oscarina, Sylvia Wyanda Arnafia, Wyanda